Abstract:
:Day, L. E. (Chas. Pfizer & Co., Inc., Groton, Conn.). Tetracycline inhibition of cell-free protein synthesis. II. Effect of the binding of tetracycline to the components of the system. J. Bacteriol. 92:197-203. 1966.-When tetracycline, an inhibitor of cell-free protein synthesis, was preincubated with each component of the Escherichia coli cell-free system, i.e., ribosomes, soluble ribonucleic acid (sRNA), polyuridylic acid (poly U), and S-100 (supernatant enzymes), only the ribosomal-bound antibiotic was inhibitory to the cell-free assay. Experiments designed to further localize the site of inhibition to either the 50S (Svedberg) or the 30S ribosomal subunit were not conclusive. Tritiated tetracycline (7-H(3)-tetracycline) was bound to isolated 50S ribosomes, and these were recombined with 30S subunits to form 70S ribosomes. When these ribosomes were dissociated and the subunits reisolated, the antibiotic was found with both the 50S and the 30S particles. The same results were observed when the tetracycline was initially bound to the 30S subunit.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Day LEdoi
10.1128/JB.92.1.197-203.1966subject
Has Abstractpub_date
1966-07-01 00:00:00pages
197-203issue
1eissn
0021-9193issn
1098-5530journal_volume
92pub_type
杂志文章abstract::A gene specifying the enzyme nicotinamide deamidase has been mapped, by the interrupted mating technique, at about 33 min on the chromosome of Escherichia coli K-12. ...
journal_title:Journal of bacteriology
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doi:10.1128/JB.101.3.1090-1091.1970
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更新日期:1969-07-01 00:00:00
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更新日期:1978-04-01 00:00:00
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更新日期:1994-06-01 00:00:00
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更新日期:2003-01-01 00:00:00
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更新日期:1966-08-01 00:00:00
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更新日期:1980-10-01 00:00:00
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更新日期:2019-10-04 00:00:00
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更新日期:2005-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2007-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1974-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:1972-01-01 00:00:00