Tetracycline inhibition of cell-free protein synthesis. II. Effect of the binding of tetracycline to the components of the system.

Abstract:

:Day, L. E. (Chas. Pfizer & Co., Inc., Groton, Conn.). Tetracycline inhibition of cell-free protein synthesis. II. Effect of the binding of tetracycline to the components of the system. J. Bacteriol. 92:197-203. 1966.-When tetracycline, an inhibitor of cell-free protein synthesis, was preincubated with each component of the Escherichia coli cell-free system, i.e., ribosomes, soluble ribonucleic acid (sRNA), polyuridylic acid (poly U), and S-100 (supernatant enzymes), only the ribosomal-bound antibiotic was inhibitory to the cell-free assay. Experiments designed to further localize the site of inhibition to either the 50S (Svedberg) or the 30S ribosomal subunit were not conclusive. Tritiated tetracycline (7-H(3)-tetracycline) was bound to isolated 50S ribosomes, and these were recombined with 30S subunits to form 70S ribosomes. When these ribosomes were dissociated and the subunits reisolated, the antibiotic was found with both the 50S and the 30S particles. The same results were observed when the tetracycline was initially bound to the 30S subunit.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Day LE

doi

10.1128/JB.92.1.197-203.1966

subject

Has Abstract

pub_date

1966-07-01 00:00:00

pages

197-203

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

92

pub_type

杂志文章
  • Chromosomal location of a gene defining nicotinamide deamidase in Escherichia coli.

    abstract::A gene specifying the enzyme nicotinamide deamidase has been mapped, by the interrupted mating technique, at about 33 min on the chromosome of Escherichia coli K-12. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.101.3.1090-1091.1970

    authors: Dickinson ES,Sundaram TK

    更新日期:1970-03-01 00:00:00

  • Biochemical characterization of UDP-Gal:GlcNAc-pyrophosphate-lipid β-1,4-Galactosyltransferase WfeD, a new enzyme from Shigella boydii type 14 that catalyzes the second step in O-antigen repeating-unit synthesis.

    abstract::The O antigen is the outer part of the lipopolysaccharide (LPS) in the outer membrane of Gram-negative bacteria and contains many repeats of an oligosaccharide unit. It contributes to antigenic variability and is essential to the full function and virulence of bacteria. Shigella is a Gram-negative human pathogen that ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00737-10

    authors: Xu C,Liu B,Hu B,Han Y,Feng L,Allingham JS,Szarek WA,Wang L,Brockhausen I

    更新日期:2011-01-01 00:00:00

  • ZipA-induced bundling of FtsZ polymers mediated by an interaction between C-terminal domains.

    abstract::FtsZ and ZipA are essential components of the septal ring apparatus, which mediates cell division in Escherichia coli. FtsZ is a cytoplasmic tubulin-like GTPase that forms protofilament-like homopolymers in vitro. In the cell, the protein assembles into a ring structure at the prospective division site early in the di...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.18.5153-5166.2000

    authors: Hale CA,Rhee AC,de Boer PA

    更新日期:2000-09-01 00:00:00

  • Biosynthesis of wall polymers in Bacillus subtilis.

    abstract::Preparations of membrane plus wall derived from Bacillus subtilis W23 were used to study the in vitro synthesis of peptidoglycan and teichoic acid and their linkage to the preexisting cell wall. The teichoic acid synthesis showed an ordered requirement for the incorporation of N-acetylglucosamine from uridine 5'-dipho...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.130.3.1055-1063.1977

    authors: Wyke AW,Ward JB

    更新日期:1977-06-01 00:00:00

  • Role of the CheW protein in bacterial chemotaxis: overexpression is equivalent to absence.

    abstract::The cheW gene from Escherichia coli has been cloned an inducible promoter, and the effects of the overproduction of the CheW protein on chemotactic behavior and receptor covalent modification have been examined. Plasmids that contain the cheW gene behind a regulatable promoter complement a cheW mutation when the CheW ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.11.6271-6278.1989

    authors: Sanders DA,Mendez B,Koshland DE Jr

    更新日期:1989-11-01 00:00:00

  • Organization of enzymes in the polyaromatic synthetic pathway: separability in bacteria.

    abstract::ULTRACENTRIFUGATION IN SUCROSE DENSITY GRADIENTS WAS EMPLOYED TO ESTIMATE THE MOLECULAR WEIGHTS AND TO DETERMINE POSSIBLE PHYSICAL AGGREGATION OF THE FIVE ENZYMES CATALYZING STEPS TWO TO SIX IN THE PRECHORISMIC ACID PORTION OF THE POLYAROMATIC SYNTHETIC PATHWAY IN SIX SPECIES OF BACTERIA: Escherichia coli, Salmonella ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.99.1.222-230.1969

    authors: Berlyn MB,Giles NH

    更新日期:1969-07-01 00:00:00

  • Glucose dehydrogenation in bacteria: a comparative study.

    abstract::Hauge, Jens G. (National Institute for Public Health, Oslo, Norway). Glucose dehydrogenation in bacteria: a comparative study. J. Bacteriol. 82:609-614. 1961.-Extracts of a series of bacteria reported to convert glucose to gluconic acid via particulate enzyme systems were fractionated by differential centrifugation in...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.82.4.609-614.1961

    authors: HAUGE JG

    更新日期:1961-10-01 00:00:00

  • Assimilatory sulfate reduction in an Escherichia coli mutant lacking thioredoxin activity.

    abstract::An investigation of sulfate reduction in B tsnC*7004, a mutant of Escherichia coli lacking thioredoxin, is reported. Although thioredoxin is indispensable for the adenosine 3'-phosphate 5'-phosphosulfate (PAPS) sulfotransferase reaction under the usual conditions of assay in extracts of wild-type cells, the mutant gre...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.134.1.131-138.1978

    authors: Tsang ML,Schiff JA

    更新日期:1978-04-01 00:00:00

  • Activation of spo0A transcription by sigma H is necessary for sporulation but not for competence in Bacillus subtilis.

    abstract::spo0A and spo0H are needed for the initiation of sporulation and for the development of genetic competence in Bacillus subtilis. Transcription of spo0A initiates from two promoters, Pv and Ps. Pv is active during vegetative growth and is recognized by RNA polymerase containing sigma A. Expression from Ps increases dur...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.12.3812-3815.1994

    authors: Siranosian KJ,Grossman AD

    更新日期:1994-06-01 00:00:00

  • Sequence and transcriptional start site of the Pseudomonas aeruginosa outer membrane porin protein F gene.

    abstract::Porin F is one of the major proteins of the outer membrane of Pseudomonas aeruginosa. It forms water-filled pores of variable size. Porin F is a candidate for a vaccine against P. aeruginosa because it antigenically cross-reacts in all serotype strains of the International Antigenic Typing Scheme. We have isolated the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.1.155-162.1988

    authors: Duchêne M,Schweizer A,Lottspeich F,Krauss G,Marget M,Vogel K,von Specht BU,Domdey H

    更新日期:1988-01-01 00:00:00

  • Growth of mycobacteria on carbon monoxide and methanol.

    abstract::Several mycobacterial strains, such as Mycobacterium flavescens, Mycobacterium gastri, Mycobacterium neoaurum, Mycobacterium parafortuitum, Mycobacterium peregrinum, Mycobacterium phlei, Mycobacterium smegmatis, Mycobacterium tuberculosis, and Mycobacterium vaccae, were found to grow on carbon monoxide (CO) as the sol...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.1.142-147.2003

    authors: Park SW,Hwang EH,Park H,Kim JA,Heo J,Lee KH,Song T,Kim E,Ro YT,Kim SW,Kim YM

    更新日期:2003-01-01 00:00:00

  • Operator sequences of the aerobactin operon of plasmid ColV-K30 binding the ferric uptake regulation (fur) repressor.

    abstract::The promoter region of the pColV-K30-encoded operon specifying biosynthesis and transport of the siderophore aerobactin was subjected to deletion analysis to determine the smallest DNA sequence affording iron regulation of a iucA'-'lacZ gene fusion. A 78-base-pair (bp) region containing the main (P1) promoter retained...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.6.2624-2630.1987

    authors: de Lorenzo V,Wee S,Herrero M,Neilands JB

    更新日期:1987-06-01 00:00:00

  • The C-Terminal Zwitterionic Sequence of CotB1 Is Essential for Biosilicification of the Bacillus cereus Spore Coat.

    abstract:UNLABELLED:Silica is deposited in and around the spore coat layer of Bacillus cereus, and enhances the spore's acid resistance. Several peptides and proteins, including diatom silaffin and silacidin peptides, are involved in eukaryotic silica biomineralization (biosilicification). Homologous sequence search revealed a ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00447-15

    authors: Motomura K,Ikeda T,Matsuyama S,Abdelhamid MA,Tanaka T,Ishida T,Hirota R,Kuroda A

    更新日期:2015-10-26 00:00:00

  • Genetic characterization of a novel rhizobial plasmid conjugation system in Rhizobium leguminosarum bv. viciae strain VF39SM.

    abstract::Rhizobium leguminosarum strain VF39SM contains two plasmids that have previously been shown to be self-transmissible by conjugation. One of these plasmids, pRleVF39b, is shown in this study to carry a set of plasmid transfer genes that differs significantly from conjugation systems previously studied in the rhizobia b...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01234-12

    authors: Ding H,Yip CB,Hynes MF

    更新日期:2013-01-01 00:00:00

  • Sequencing and characterization of a gene cluster encoding the enzymes for L-rhamnose metabolism in Escherichia coli.

    abstract::The sequencing of the EcoRI-HindIII fragment complementing mutations in the structural genes of the L-rhamnose regulon of Escherichia coli has permitted identification of the open reading frames corresponding to rhaB, rhaA, and rhaD. The deduced amino acid sequences gave a 425-amino-acid polypeptide corresponding to r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.17.5585-5594.1993

    authors: Moralejo P,Egan SM,Hidalgo E,Aguilar J

    更新日期:1993-09-01 00:00:00

  • Characterization of a Bacillus subtilis sporulation operon that includes genes for an RNA polymerase sigma factor and for a putative DD-carboxypeptidase.

    abstract::At early stages of sporulation, the spoIIA locus is transcribed as a tricistronic (1.7-kb) operon, coding for sigma F and for two proteins that modulate the activity of sigma F. The locus is transcribed as a longer (2.9-kb) transcript at the late stages of sporulation. We show here that the longer transcript contains ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.15.4885-4892.1992

    authors: Wu JJ,Schuch R,Piggot PJ

    更新日期:1992-08-01 00:00:00

  • Factors Affecting the Survival of Auxotrophs and Prototrophs of Saccharomyces cerevisiae in Mixed Populations.

    abstract::Moat, Albert G. (Hahnemann Medical College, Philadelphia, Pa.), Isabel J. Barnes, and Eleanor H. McCurley. Factors affecting the survival of auxotrophs and prototrophs of Saccharomyces cerevisiae in mixed populations. J. Bacteriol. 92:297-301. 1966.-The conditions under which the number of yeast prototrophs, as well a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.92.2.297-301.1966

    authors: Moat AG,Barnes IJ,McCurley EH

    更新日期:1966-08-01 00:00:00

  • New temperature-sensitive alleles of ftsZ in Escherichia coli.

    abstract::We isolated five new temperature-sensitive alleles of the essential cell division gene ftsZ in Escherichia coli, using P1-mediated, localized mutagenesis. The five resulting single amino acid changes (Gly109-->Ser109 for ftsZ6460, Ala129-->Thr129 for ftsZ972, Val157-->Met157 for ftsZ2066, Pro203-->Leu203 for ftsZ9124,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.1.358-365.2005

    authors: Addinall SG,Small E,Whitaker D,Sturrock S,Donachie WD,Khattar MM

    更新日期:2005-01-01 00:00:00

  • Rok regulates yuaB expression during architecturally complex colony development of Bacillus subtilis 168.

    abstract::Transcriptome analysis of a Bacillus subtilis rok strain that showed reduced complex colony structure formation revealed significant downregulation of the yuaB gene. Overexpression of yuaB restored structure formation in the rok strain. We show that transcription of yuaB is indirectly regulated by Rok, independently f...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01170-10

    authors: Kovács AT,Kuipers OP

    更新日期:2011-02-01 00:00:00

  • The serine protease HhoA from Synechocystis sp. strain PCC 6803: substrate specificity and formation of a hexameric complex are regulated by the PDZ domain.

    abstract::Enzymes of the ATP-independent Deg serine endopeptidase family are very flexible with regard to their substrate specificity. Some family members cleave only one substrate, while others act as general proteases on unfolded substrates. The proteolytic activity of Deg proteases is regulated by PDZ protein interaction dom...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00883-07

    authors: Huesgen PF,Scholz P,Adamska I

    更新日期:2007-09-01 00:00:00

  • Molecular characterization of "plasmid-free" antibiotic-resistant Haemophilus influenzae.

    abstract::We examined 14 multiresistant and 8 ampicillin- or tetracycline-resistant Haemophilus influenzae isolates and 4 ampicillin-resistant H. parainfluenzae isolates for plasmid deoxyribonucleic acid. Sixteen strains carried plasmids. Both "plasmid-free" and plasmid-carrying isolates transferred the antibiotic resistance by...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.144.1.476-479.1980

    authors: Roberts MC,Smith AL

    更新日期:1980-10-01 00:00:00

  • Importance of Individual Germination Receptor Subunits in the Cooperative Function between GerA and Ynd.

    abstract::Germination of Bacillus spores is triggered by the binding of specific nutrients to germinant receptors (GRs) located in the spore's inner membrane. The GRs typically consist of A, B, and C subunits, encoded by tricistronic ger operons. The Bacillus licheniformis genome contains the gerA family operons gerA, ynd, and ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00451-19

    authors: Aspholm M,Borch-Pedersen K,O'Sullivan K,Fjellheim S,Aardal IB,Granum PE,Lindbäck T

    更新日期:2019-10-04 00:00:00

  • Properties of pGC1, a lac plasmid orginating in Yersinia enterocolitica 842.

    abstract::pGC1, a self-transmissible lac plasmid that originated in Yersinia enterocolitica 842, is described. This plasmid is freely transmissible between strains of Y. enterocolitica and from such strains to strains of Escherichia coli. The plasmid can also be transferref among G. coli strains and back to Y. enterocolitica, a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.127.3.1058-1062.1976

    authors: Cornelis G,Bennett PM,Grinsted J

    更新日期:1976-09-01 00:00:00

  • Measurement of the cyanophycin granule polypeptide contained in the blue-green alga Anabaena cylindrica.

    abstract::An assay was developed to determine the amount of cyanophycin granules in blue-green algae. The amount of this polypeptide in cells of Anabaena cylindrica was measured as a function of culture age and was compared with changes in other proteinaceous cellular components. The data presented support the notion that the c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.114.3.1213-1216.1973

    authors: Simon RD

    更新日期:1973-06-01 00:00:00

  • Nucleotide sequence analysis and expression of the minimum REPI replication region and incompatibility determinants of pColV-K30.

    abstract::We sequenced the minimum REPI replication region and the incompatibility determinants of pColV-K30. The minimum replication region contains an open reading frame which corresponds to a 35-kilodalton (kDa) protein. Sodium dodecyl sulfate-polyacrylamide gel electrophoretic analysis with maxicells transformed with a numb...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.4.2195-2201.1989

    authors: Perez-Casal JF,Gammie AE,Crosa JH

    更新日期:1989-04-01 00:00:00

  • Purification, properties, and regulation of glutamic dehydrogenase of Bacillus licheniformis.

    abstract::Cell-free extracts of Bacillus licheniformis and B. cereus were found to contain high specific activities of nicotinamide adenine dinucleotide phosphate (NADP)-dependent-l-glutamate dehydrogenase [EC 1.4.1.4; l-glutamate: NADP oxidoreductase (deaminating)]. Maximum specific activities were found in extracts of cells d...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.106.2.375-385.1971

    authors: Phibbs PV Jr,Bernlohr RW

    更新日期:1971-05-01 00:00:00

  • Menaquinone-specific prenyl reductase from the hyperthermophilic archaeon Archaeoglobus fulgidus.

    abstract::Four genes that encode the homologues of plant geranylgeranyl reductase were isolated from a hyperthermophilic archaeon Archaeoglobus fulgidus, which produces menaquinone with a fully saturated heptaprenyl side chain, menaquinone-7(14H). The recombinant expression of one of the homologues in Escherichia coli led to a ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.6.1937-1944.2005

    authors: Hemmi H,Takahashi Y,Shibuya K,Nakayama T,Nishino T

    更新日期:2005-03-01 00:00:00

  • Interactions between the lipoprotein PilP and the secretin PilQ in Neisseria meningitidis.

    abstract::Neisseria meningitidis can be the causative agent of meningitis or septicemia. This bacterium expresses type IV pili, which mediate a variety of functions, including autoagglutination, twitching motility, biofilm formation, adherence, and DNA uptake during transformation. The secretin PilQ supports type IV pilus extru...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00060-07

    authors: Balasingham SV,Collins RF,Assalkhou R,Homberset H,Frye SA,Derrick JP,Tønjum T

    更新日期:2007-08-01 00:00:00

  • Ribonucleic acid regulation in premeabilized cells of Escherichia coli capable of ribonucleic acid and protein synthesis.

    abstract::A cell permeabilization procedure is described that reduces viability less than 10% and does not significantly reduce the rates of ribonucleic acid and protein synthesis when appropriately supplemented. Permeabilization abolishes the normal stringent coupling of protein and ribonucleic acid synthesis. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.118.3.1186-1189.1974

    authors: Atherly AG

    更新日期:1974-06-01 00:00:00

  • Loss of the penicillinase plasmid after treatment of Staphylococcus aureus with sodium dodecyl sulfate.

    abstract::Two strains of Staphylococcus aureus (PC1 and 196E), when grown in medium containing 0.002% sodium dodecyl sulfate (SDS), showed from 96.1 to 100% loss of the ability to produce penicillinase. Resistance to cadmium and zinc was lost concomitantly with the ability to produce penicillinase. A comparison of the rate of c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.109.1.262-265.1972

    authors: Sonstein SA,Baldwin JN

    更新日期:1972-01-01 00:00:00