Purification, properties, and regulation of glutamic dehydrogenase of Bacillus licheniformis.

Abstract:

:Cell-free extracts of Bacillus licheniformis and B. cereus were found to contain high specific activities of nicotinamide adenine dinucleotide phosphate (NADP)-dependent-l-glutamate dehydrogenase [EC 1.4.1.4; l-glutamate: NADP oxidoreductase (deaminating)]. Maximum specific activities were found in extracts of cells during the late exponential phase of growth when ammonium ion served as the sole source of nitrogen. Extremely low specific activities were detected throughout the growth cycle when l-glutamate or Casamino Acids served as the source of carbon and nitrogen. The enzyme was purified 55-fold from crude extracts of B. licheniformis, and apparent kinetic constants were determined. Sigmoidal saturation kinetics were not observed, and various adenylates had no effect on the enzyme. Repression of enzyme synthesis during growth on l-glutamate or Casamino Acids was partially overcome by additions of glucose or pyruvate, and this apparent derepression was totally abolished by inhibitors of ribonucleic acid and protein synthesis. Similarly, additions of l-glutamate or Casamino Acids to cells growing on glucose-ammonium ion resulted in strong repression of enzyme synthesis. It is suggested that the enzyme serves an anabolic role in metabolism. Nicotinamide adenine dinucleotide-dependent glutamate dehydrogenase activity was not detected in five species of Bacillus, irrespective of nutritional conditions or of the physiological age of cells.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Phibbs PV Jr,Bernlohr RW

doi

10.1128/JB.106.2.375-385.1971

subject

Has Abstract

pub_date

1971-05-01 00:00:00

pages

375-85

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

106

pub_type

杂志文章
  • Colonial heterogeneity of Thiobacillus versutus.

    abstract::In acetate-limited chemostat cultures started with single-colony cultures of Thiobacillus versutus, a mutant appeared after approximately 85 volume changes. The inhomogeneity of the culture was detected by the development of two different types of colonies on agar plates. When a pure culture of the mutant was grown in...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.168.2.791-794.1986

    authors: Claassen PA,Kortstee GJ,Oosterveld-van Vliet WM,van Neerven AR

    更新日期:1986-11-01 00:00:00

  • Stimulation of transport into Escherichia coli membrane vesicles by internally generated reduced nictotinamide adenine dinucleotide.

    abstract::Membrane vesicles containing internal alcohol dehydrogenase and nicotinamide adenine dinucleotide (NAD) were prepared from Escherichia coli ML308-225. Upon the addition of ethanol, these vesicles respired and transported several amino acids. Transport rates driven by internally generated reduced NAD (NADH) were about ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.120.2.861-865.1974

    authors: Futai M

    更新日期:1974-11-01 00:00:00

  • The SPOR Domain, a Widely Conserved Peptidoglycan Binding Domain That Targets Proteins to the Site of Cell Division.

    abstract::Sporulation-related repeat (SPOR) domains are small peptidoglycan (PG) binding domains found in thousands of bacterial proteins. The name "SPOR domain" stems from the fact that several early examples came from proteins involved in sporulation, but SPOR domain proteins are quite diverse and contribute to a variety of p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章,评审

    doi:10.1128/JB.00118-17

    authors: Yahashiri A,Jorgenson MA,Weiss DS

    更新日期:2017-06-27 00:00:00

  • Molecular mechanism of tryptophan-dependent transcriptional regulation in Chlamydia trachomatis.

    abstract::Tryptophan is an essential amino acid that is required for normal development in Chlamydia species, and tryptophan metabolism has been implicated in chlamydial persistence and tissue tropism. The ability to synthesize tryptophan is not universal among the Chlamydiaceae, but species that have a predicted tryptophan bio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01660-05

    authors: Akers JC,Tan M

    更新日期:2006-06-01 00:00:00

  • Isolation of a novel protein involved in the transport of fructose by an inducible phosphoenolpyruvate fructose phosphotransferase system in Streptococcus mutans.

    abstract::Fructose transport in Streptococcus mutans LG-1 is mediated by at least two distinct phosphoenolpyruvate fructose phosphotransferase systems. One system is constitutive and consists of membrane components enzyme II as well as enzyme I and heat-stable protein. The other system is inducible and requires, in addition to ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.160.2.755-763.1984

    authors: Gauthier L,Mayrand D,Vadeboncoeur C

    更新日期:1984-11-01 00:00:00

  • A novel role for Escherichia coli endonuclease VIII in prevention of spontaneous G-->T transversions.

    abstract::In the bacterium Escherichia coli, oxidized pyrimidines are removed by two DNA glycosylases, endonuclease III and endonuclease VIII (endo VIII), encoded by the nth and nei genes, respectively. Double mutants lacking both of these activities exhibit a high spontaneous mutation frequency, and here we show that all of th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.20.6396-6402.1999

    authors: Blaisdell JO,Hatahet Z,Wallace SS

    更新日期:1999-10-01 00:00:00

  • IS1634, a novel insertion element creating long, variable-length direct repeats which is specific for Mycoplasma mycoides subsp. mycoides small-colony type.

    abstract::A new insertion sequence, IS1634, has been identified in Mycoplasma mycoides subsp. mycoides small-colony type (SC). IS1634 shows structural and functional similarities to IS1549 of Mycobacterium smegmatis and with it seems to form a new class or family of insertion sequences. IS1634 has a size of 1,872 bp, including ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.4.1319-1323.1999

    authors: Vilei EM,Nicolet J,Frey J

    更新日期:1999-02-01 00:00:00

  • Increased resistance to chloramphenicol in Rickettsia prowazekii with a note on failure to demonstrate genetic interaction among.

    abstract::Weiss, Emilio (Naval Medical Research Institute, Bethesda, Md.) and Harry R. Dressler. Increased resistance to chloramphenicol in Rickettsia prowazekii with a note on failure to demonstrate genetic interaction among strains. J. Bacteriol. 83:409-414. 1962.-A further effort to produce genetically labeled Rickettsia pro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.2.409-414.1962

    authors: WEISS E,DRESSLER HR

    更新日期:1962-02-01 00:00:00

  • Transcriptional regulation of a promoter in the men gene cluster of Bacillus subtilis.

    abstract::The control of men gene expression during growth and sporulation of Bacillus subtilis was examined at the transcriptional level. Two different approaches were used. (i) Steady-state levels of men-specific mRNA were measured directly. (ii) A men'-lacZ gene fusion was constructed. In both cases, it was observed that men...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.6.2742-2748.1988

    authors: Miller P,Mueller J,Hill K,Taber H

    更新日期:1988-06-01 00:00:00

  • Expression of an alternative nitrogen fixation system in Azotobacter vinelandii.

    abstract::Nitrogenase activities were determined from maximum acetylene reduction rates for mutant strains of Azotobacter vinelandii which are unable to fix N2 in the presence of molybdenum (Nif-) but undergo phenotypic reversal to Nif+ under conditions of Mo deficiency. The system responsible for N2 fixation under these condit...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.150.3.1244-1251.1982

    authors: Bishop PE,Jarlenski DM,Hetherington DR

    更新日期:1982-06-01 00:00:00

  • Archaeal intrinsic transcription termination in vivo.

    abstract::Thermococcus kodakarensis (formerly Thermococcus kodakaraensis) strains have been constructed with synthetic and natural DNA sequences, predicted to function as archaeal transcription terminators, identically positioned between a constitutive promoter and a beta-glycosidase-encoding reporter gene (TK1761). Expression ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00982-09

    authors: Santangelo TJ,Cubonová L,Skinner KM,Reeve JN

    更新日期:2009-11-01 00:00:00

  • Characterization of Key Helicobacter pylori Regulators Identifies a Role for ArsRS in Biofilm Formation.

    abstract:UNLABELLED:Helicobacter pylori must be able to rapidly respond to fluctuating conditions within the stomach. Despite this need for constant adaptation, H. pylori encodes few regulatory proteins. Of the identified regulators, the ferric uptake regulator (Fur), the nickel response regulator (NikR), and the two-component ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00324-16

    authors: Servetas SL,Carpenter BM,Haley KP,Gilbreath JJ,Gaddy JA,Merrell DS

    更新日期:2016-08-25 00:00:00

  • Isolation and expansion of the catabolic potential of a Pseudomonas putida strain able to grow in the presence of high concentrations of aromatic hydrocarbons.

    abstract::Pseudomonas putida DOT-T1 was isolated after enrichment on minimal medium with 1% (vol/vol) toluene as the sole C source. The strain was able to grow in the presence of 90% (vol/vol) toluene and was tolerant to organic solvents whose log P(ow) (octanol/water partition coefficient) was higher than 2.3. Solvent toleranc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.14.3911-3916.1995

    authors: Ramos JL,Duque E,Huertas MJ,Haïdour A

    更新日期:1995-07-01 00:00:00

  • Regulation of arylsulfatase synthesis by sulfur compounds in Klebsiella aerogenes.

    abstract::In Klebsiella aerogenes, arylsulfatase synthesis was repressed by inorganic sulfate, sulfite, sulfide, thiosulfate, and cysteine, but not by methionine under normal growth conditions. We isolated cysteine-requiring mutants (Cys minus), and mutants (AtsS minus, AtsR minus) in which the regulation of arylsulfatase synth...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.121.1.29-35.1975

    authors: Adachi T,Murooka Y,Harada T

    更新日期:1975-01-01 00:00:00

  • The integrase of the conjugative transposon Tn916 directs strand- and sequence-specific cleavage of the origin of conjugal transfer, oriT, by the endonuclease Orf20.

    abstract::Orf20 of the conjugative transposon Tn916 was purified as a chimeric protein fused to maltose binding protein (MBP-Orf20). The chimeric protein possessed endonucleolytic activity, cleaving both strands of the Tn916 origin of conjugal transfer (oriT) at several distinct sites and favoring GT dinucleotides. Incubation o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.6.2207-2213.2006

    authors: Rocco JM,Churchward G

    更新日期:2006-03-01 00:00:00

  • Purification and properties of glycogen isolated from a blue-green alga, Nostoc muscorum.

    abstract::Alpha granules were isolated from a blue-green alga, Nostoc muscorum, in large quantities and high purity by sucrose density gradient centrifugation. Sodium deoxycholate was used to eliminate membrane contamination. Isolated alpha granules from this species have average dimension of 31 nm in width and 65 nm in length....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.105.1.331-338.1971

    authors: Chao L,Bowen CC

    更新日期:1971-01-01 00:00:00

  • Occurrence of glutathione in bacteria.

    abstract::Glutathione and soluble thiol content were examined in a broad spectrum of bacteria. Significant soluble thiol was present in all cases. The thiol compound was glutathione in most of the gram-negative bacteria but not in most of the gram-positive bacteria studied. Glutathione was absent in four anerobes and one microa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.133.3.1126-1129.1978

    authors: Fahey RC,Brown WC,Adams WB,Worsham MB

    更新日期:1978-03-01 00:00:00

  • Return of Streptococcus faecalis DNA cloned in Escherichia coli to its original host via transformation of Streptococcus sanguis followed by conjugative mobilization.

    abstract::Cloning vectors were introduced into Streptococcus faecalis by conjugation. A conjugative plasmid (pVA797) and cloning vector pVA838 recombined in Streptococcus sanguis at homologous sequences, forming a cointegrate. The pVA797::pVA838 cointegrate transferred to S. faecalis by conjugation. Recombination between homolo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.160.3.1109-1114.1984

    authors: Smith MD,Clewell DB

    更新日期:1984-12-01 00:00:00

  • Novel toluene elimination system in a toluene-tolerant microorganism.

    abstract::In studies of Pseudomonas putida IH-2000, a toluene-tolerant microorganism, membrane vesicles (MVs) were found to be released from the outer membrane when toluene was added to the culture. These MVs were found to be composed of phospholipids, lipopolysaccharides (LPS), and very low amounts of outer membrane proteins. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.22.6451-6455.2000

    authors: Kobayashi H,Uematsu K,Hirayama H,Horikoshi K

    更新日期:2000-11-01 00:00:00

  • Conserved cytoplasmic loops are important for both the transport and chemotaxis functions of PcaK, a protein from Pseudomonas putida with 12 membrane-spanning regions.

    abstract::Chemotaxis to the aromatic acid 4-hydroxybenzoate (4-HBA) by Pseudomonas putida is mediated by PcaK, a membrane-bound protein that also functions as a 4-HBA transporter. PcaK belongs to the major facilitator superfamily (MFS) of transport proteins, none of which have so far been implicated in chemotaxis. Work with two...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.16.5068-5074.1999

    authors: Ditty JL,Harwood CS

    更新日期:1999-08-01 00:00:00

  • Genome sequence of a serotype b non-JP2 aggregatibacter actinomycetemcomitans strain, ANH9381, from a periodontally healthy individual.

    abstract::Gram-negative Aggregatibacter actinomycetemcomitans can be distinguished (based on the promoter structure of the leukotoxin operon) into JP2 and non-JP2 genotypes, with the former found to be more pathogenic than the latter. Here we report the first complete genome sequence of a serotype b non-JP2 strain of A. actinom...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06770-11

    authors: Chen C,Kittichotirat W,Chen W,Downey JS,Bumgarner R

    更新日期:2012-04-01 00:00:00

  • Characterization of two glyceraldehyde-3-phosphate dehydrogenase isoenzymes from the pentalenolactone producer Streptomyces arenae.

    abstract::Pentalenolactone (PL) irreversibly inactivates the enzyme glyceraldehyde-3-phosphate dehydrogenase [D-glyceraldehyde-3-phosphate:NAD+ oxidoreductase (phosphorylating)] (EC 1.2.1.12) and thus is a potent inhibitor of glycolysis in both procaryotic and eucaryotic cells. We showed that PL-producing strain Streptomyces ar...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.153.2.930-936.1983

    authors: Maurer KH,Pfeiffer F,Zehender H,Mecke D

    更新日期:1983-02-01 00:00:00

  • Topology of the membrane protein LamB by epitope tagging and a comparison with the X-ray model.

    abstract::We previously developed a genetic approach to study, with a single antibody, the topology of the outer membrane protein LamB, an Escherichia coli porin with specificity towards maltodextrins and a receptor for bacteriophage lambda. Our initial procedure consisted of inserting at random the same reporter epitope (the C...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.12.3447-3456.1996

    authors: Newton SM,Klebba PE,Michel V,Hofnung M,Charbit A

    更新日期:1996-06-01 00:00:00

  • The URE2 protein regulates nitrogen catabolic gene expression through the GATAA-containing UASNTR element in Saccharomyces cerevisiae.

    abstract::Many of the gene products that participate in nitrogen metabolism are sensitive to nitrogen catabolite repression (NCR), i.e., their expression is decreased to low levels when readily used nitrogen sources such as asparagine are provided. Previous work has shown this NCR sensitivity requires the cis-acting UASNTR elem...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.24.7476-7483.1994

    authors: Coffman JA,el Berry HM,Cooper TG

    更新日期:1994-12-01 00:00:00

  • Permeability of bacterial spores. II. Molecular variables affecting solute permeation.

    abstract::Gerhardt, Philipp (University of Michigan, Ann Arbor) and S. H. Black. Permeability of bacterial spores. II. Molecular variables affecting solute permeation. J. Bacteriol. 82:750-760. 1961.-More than 100 compounds were tested for their uptake by dormant spores of a bacillus. The extent of penetration was found to be d...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.82.5.750-760.1961

    authors: GERHARDT P,BLACK SH

    更新日期:1961-11-01 00:00:00

  • ECOLOGY OF NASAL STAPHYLOCOCCI.

    abstract::Davis, Nour A. (University of Lagos Medical School, Lagos, Nigeria), and G. H. G. Davis. Ecology of nasal staphylococci. J. Bacteriol. 89:1163-1168. 1965.-The rate of nasal carriage of Staphylococcus aureus in Nigerian adults (46%) approximates that found in other countries. The rate in infants under 12 months was ca....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.89.5.1163-1168.1965

    authors: DAVIS NA,DAVIS GH

    更新日期:1965-05-01 00:00:00

  • Genome sequence of Reyranella massiliensis, a bacterium associated with amoebae.

    abstract::Reyranella massiliensis is an Alphaproteobacterium member of the class Rhodospirillaceae, growing in amoebae. We sequenced the genome of type strain 521(T). It is composed of a 5,792,218-bp chromosome and encodes 5,675 protein-coding genes and 53 RNA genes, including 3 rRNA genes. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01260-12

    authors: Pagnier I,Croce O,Robert C,Raoult D,La Scola B

    更新日期:2012-10-01 00:00:00

  • Reduce, Induce, Thrive: Bacterial Redox Sensing during Pathogenesis.

    abstract::The abundance of oxidants and reductants must be balanced for an organism to thrive. Bacteria have evolved methods to prevent redox imbalances and to mitigate their deleterious consequences through the expression of detoxification enzymes, antioxidants, and systems to repair or degrade damaged proteins and DNA. Regula...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章,评审

    doi:10.1128/JB.00128-18

    authors: Reniere ML

    更新日期:2018-08-10 00:00:00

  • Role of the Transporter-Like Sensor Kinase CbrA in Histidine Uptake and Signal Transduction.

    abstract:UNLABELLED:CbrA is an atypical sensor kinase found in Pseudomonas. The autokinase domain is connected to a putative transporter of the sodium/solute symporter family (SSSF). CbrA functions together with its cognate response regulator, CbrB, and plays an important role in nutrient acquisition, including regulation of hu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00361-15

    authors: Zhang XX,Gauntlett JC,Oldenburg DG,Cook GM,Rainey PB

    更新日期:2015-09-01 00:00:00

  • Characterization of the genes and proteins of a two-component system from the hyperthermophilic bacterium Thermotoga maritima.

    abstract::As a step towards studying representative members of the two-component family of signal transduction proteins, we have cloned genes encoding a histidine protein kinase and a response regulator from the hyperthermophilic bacterium Thermotoga maritima. The genes have been designated HpkA and drrA, respectively. The dedu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.19.5579-5585.1996

    authors: Lee PJ,Stock AM

    更新日期:1996-10-01 00:00:00