Stimulation of transport into Escherichia coli membrane vesicles by internally generated reduced nictotinamide adenine dinucleotide.

abstract：:The lining of the maltodextrin-specific maltoporin (LamB) channel exhibits a string of aromatic residues, the greasy slide, part of which has been shown previously by crystallography to be involved in substrate binding. To probe the functional role of the greasy slide, alanine scanning mutagenesis has been performed o...

journal_title：Journal of bacteriology

authors： Van Gelder P,Dumas F,Bartoldus I,Saint N,Prilipov A,Winterhalter M,Wang Y,Philippsen A,Rosenbusch JP,Schirmer T

更新日期：2002-06-01 00:00:00

abstract：:A positive selection method for isolation of nitrogenase-derepressed mutant strains of a filamentous cyanobacterium, Anabaena variabilis, is described. Mutant strains that are resistant to a glutamate analog, L-methionine-D,L-sulfoximine, were screened for their ability to produce and excrete NH4+ into medium. Mutant ...

journal_title：Journal of bacteriology

authors： Spiller H,Latorre C,Hassan ME,Shanmugam KT

更新日期：1986-02-01 00:00:00

abstract：:RpoS is a conserved alternative sigma factor that regulates the expression of many stress response genes in Escherichia coli. The RpoS regulon is large but has not yet been completely characterized. In this study, we report the identification of over 100 RpoS-dependent fusions in a genetic screen based on the differen...

journal_title：Journal of bacteriology

authors： Vijayakumar SR,Kirchhof MG,Patten CL,Schellhorn HE

更新日期：2004-12-01 00:00:00

abstract：:Production of Bacillus subtilis exoproteases is positively regulated by the DegS-DegU two-component regulatory system and other regulatory factors including DegR and ProB. It was shown that the expression of degR was virtually abolished in a sigD mutant and that the transcriptional initiation site in vivo is preceded ...

journal_title：Journal of bacteriology

authors： Ogura M,Tanaka T

更新日期：1996-01-01 00:00:00

abstract：:Cultures of a stable L-phase variant of Streptococcus faecium F24 produced and retained peptidoglycan precursors intracellularly over the entire growth cycle in a chemically defined medium. The identity of the most abundant precursor, UDP N-acetylmuramyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine (UDP-MurNAc-penta...

journal_title：Journal of bacteriology

authors： Gregory WW,Gooder H

更新日期：1978-09-01 00:00:00

abstract：:Klebsiella pneumoniae can use nitrate and nitrite as sole nitrogen sources through the nitrate assimilatory pathway. The structural genes for assimilatory nitrate and nitrite reductases together with genes necessary for nitrate transport form an operon, nasFEDCBA. Expression of the nasF operon is regulated both by gen...

journal_title：Journal of bacteriology

authors： Goldman BS,Lin JT,Stewart V

更新日期：1994-08-01 00:00:00

abstract：:One of the phosphatidyl glycerophosphate phosphatase genes of Escherichia coli, pgpA, was cloned, and its DNA sequence was determined. Its 507-base-pair open reading frame was consistent with the 18,000-molecular-weight product identified by a maxicell experiment. Between its possible promoter and methionine initiatio...

journal_title：Journal of bacteriology

authors： Icho T

更新日期：1988-11-01 00:00:00

abstract：:We have constructed a series of broad-host-range plasmids which use "visual screens" to detect promoter activity. These plasmids contain the pMB1 and pRO1600 origins of replication and are capable of replicating in a wide range of gram-negative bacteria. The genes encoding beta-galactosidase and alkaline phosphatase f...

journal_title：Journal of bacteriology

authors： Farinha MA,Kropinski AM

更新日期：1990-06-01 00:00:00

abstract：:We previously described mutations in a gene, secB, which have pleiotropic effects on protein export in Escherichia coli. In this paper, we report the isolation of mutants in which the activity of the secB gene was eliminated. Null mutations in secB affected only a subset of exported proteins. Strains carrying these mu...

journal_title：Journal of bacteriology

authors： Kumamoto CA,Beckwith J

更新日期：1985-07-01 00:00:00

abstract：:Soluble reduced nicotinamide adenine dinucleotide oxidase activity in extracts of Bacillus subtilis spores was stimulated by the addition of not only flavine mononucleotide (FMN) or flavine adenine dinucleotide (FAD) but also alpha-alpha'-dipyridyl or o-phenanthroline. These chelating agents showed stronger effect on ...

journal_title：Journal of bacteriology

authors： Tochikubo K

更新日期：1974-03-01 00:00:00

abstract：:The Alcaligenes eutrophus JMP134 plasmid pJP4 contains genes necessary for the complete degradation of 2,4-dichlorophenoxyacetic acid (2,4-D) and 3-chlorobenzoic acid. tfdA encodes 2,4-D monooxygenase, the initial enzyme in the 2,4-D catabolic pathway. The tfdA locus has recently been localized to a region on pJP4 13 ...

journal_title：Journal of bacteriology

authors： Perkins EJ,Lurquin PF

更新日期：1988-12-01 00:00:00

abstract：:Several characteristics of Mycobacterium tuberculosis (e.g., conserved genome and low growth rate) have severely restricted the study of the microorganism. The discovery of IS6110 raised hopes of overcoming these obstacles. However, our knowledge of this IS element is relatively limited; even its two basic characteris...

journal_title：Journal of bacteriology

authors： Fang Z,Kenna DT,Doig C,Smittipat DN,Palittapongarnpim P,Watt B,Forbes KJ

更新日期：2001-09-01 00:00:00

abstract：:The Dps-like peroxide resistance protein (Dpr) is essential for H2O2 stress tolerance and aerobic growth of the oral pathogen Streptococcus mutans Dpr accumulates during oxidative stress, protecting the cell by sequestering iron ions and thereby preventing the generation of toxic hydroxyl radicals that result from the...

journal_title：Journal of bacteriology

authors： Ganguly T,Kajfasz JK,Miller JH,Rabinowitz E,Galvão LCC,Rosalen PL,Abranches J,Lemos JA

更新日期：2018-06-25 00:00:00

abstract：:The response of Neurospora to changes in the availibility of exogenous arginine was investigated. Upon addition of arginine to the growth medium, catabolism is initiated within minutes. This occurs prior to expansion of the arginine pool or augmentation of catabolic enzyme levels. (Basal levels are approximately 25% o...

journal_title：Journal of bacteriology

authors： Weiss RL,Davis RH

更新日期：1977-02-01 00:00:00

abstract：:The Salmonella plasmid virulence spvABCD genes are growth phase regulated and require RpoS for maximal expression in stationary phase. We identified a growth phase-independent expression of spv which is mediated by short-chain fatty acids. During this fatty acid-mediated expression of spv, RpoS is required for inducti...

journal_title：Journal of bacteriology

authors： El-Gedaily A,Paesold G,Chen CY,Guiney DG,Krause M

更新日期：1997-02-01 00:00:00

abstract：:A liquid chromatography-hybrid linear ion trap-Fourier transform ion cyclotron resonance mass spectrometry approach was used to determine the differential abundance of proteins in acetate-grown cells compared to that of proteins in methanol-grown cells of the marine isolate Methanosarcina acetivorans metabolically lab...

journal_title：Journal of bacteriology

authors： Li Q,Li L,Rejtar T,Lessner DJ,Karger BL,Ferry JG

更新日期：2006-01-01 00:00:00

abstract：:Kondo, Eiko (Gunma University, Maebashi, Japan), and Susumu Mitsuhashi. Drug resistance of enteric bacteria. IV. Active transducing phage P1 CM produced by the combination of R factor with phage P1. J. Bacteriol. 88:1266-1276. 1964.-During an investigation of the transduction of R factors with phage P1, a phage lysate...

journal_title：Journal of bacteriology

authors： KONDO E,MITSUHASHI S

更新日期：1964-11-01 00:00:00

abstract：:Rifkind, David (University of Colorado Medical Center, Denver), and John D. Palmer. Neutralization of endotoxin toxicity in chick embryos by antibiotics. J. Bacteriol. 92:815-819. 1966.-Three cationic cyclic polypeptide antibiotics, polymyxin B sulfate colistin sulfate, and tyrocidine hydrochloride, were shown to neut...

journal_title：Journal of bacteriology

authors： Rifkind D,Palmer JD

更新日期：1966-10-01 00:00:00

abstract：:Carotovoricin Er is a phage-tail-like bacteriocin produced by Erwinia carotovora subsp. carotovora strain Er, a causative agent for soft rot disease in plants. Here we studied binding and killing spectra of carotovoricin Er preparations for various strains of the bacterium (strains 645Ar, EC-2, N786, and P7) and found...

journal_title：Journal of bacteriology

authors： Nguyen HA,Tomita T,Hirota M,Kaneko J,Hayashi T,Kamio Y

更新日期：2001-11-01 00:00:00

abstract：:The uropathogenic Escherichia coli strain 536 carries at least five genetic elements on its chromosome that meet all criteria characteristic of pathogenicity islands (PAIs). One main feature of these distinct DNA regions is their instability. We applied the so-called island-probing approach and individually labeled al...

journal_title：Journal of bacteriology

authors： Middendorf B,Hochhut B,Leipold K,Dobrindt U,Blum-Oehler G,Hacker J

更新日期：2004-05-01 00:00:00

abstract：:In previous work, we cloned a Candida albicans cDNA for the glycolytic enzyme enolase and found a single, abundant enolase transcript on Northern (RNA) blots and a single protein on immunoblots, using antiserum raised against a recombinant enolase fusion protein. Because C. albicans enolase is abundantly produced duri...

journal_title：Journal of bacteriology

authors： Postlethwait P,Sundstrom P

更新日期：1995-04-01 00:00:00

abstract：:NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) of Paracoccus denitrificans has been purified as a tetramer with a relative molecular mass of 150 kDa. The gene encoding GD-FALDH (flhA) has been isolated, sequenced, and mutated by insertion of a kanamycin resistance gene. The mutant strain is not a...

journal_title：Journal of bacteriology

authors： Ras J,Van Ophem PW,Reijnders WN,Van Spanning RJ,Duine JA,Stouthamer AH,Harms N

更新日期：1995-01-01 00:00:00

abstract：:An assay modeled on a known polymorphism in the PE_PGRS9 gene of Mycobacterium tuberculosis was designed to assess the mutability of a sequence containing interspersed PGRS repeats. Application of the assay in Mycobacterium smegmatis revealed sequence plasticity: in addition to recapitulating the mutation on which it ...

journal_title：Journal of bacteriology

authors： Machowski EE,Barichievy S,Springer B,Durbach SI,Mizrahi V

更新日期：2007-03-01 00:00:00

abstract：:A host-vector system was developed for molecular cloning in Bacillus megaterium and used to clone the structural and immunity genes for megacins A-216 and A-19213. Recombinant clones that expressed immunity only or both immunity to and production of each megacin were obtained. Restriction mapping of native megacinogen...

journal_title：Journal of bacteriology

authors： Von Tersch MA,Carlton BC

更新日期：1984-12-01 00:00:00

abstract：:Of 16 restriction endonucleases known to hydrolyze rare 6- or 8-base recognition sequences that were tested, only SpeI, NotI, AscI, and SfiI generated fragments of chromosomal DNA from Yersinia pestis, the causative agent of bubonic plague, of sufficient length to permit physical analysis by pulsed-field gel electroph...

journal_title：Journal of bacteriology

authors： Lucier TS,Brubaker RR

更新日期：1992-04-01 00:00:00

abstract：:Marinobacter hydrocarbonoclasticus DSM 8798 has been reported to synthesize isoprenoid wax ester storage compounds when grown on phytol as the sole carbon source under limiting nitrogen and/or phosphorous conditions. We hypothesized that isoprenoid wax ester synthesis involves (i) activation of an isoprenoid fatty aci...

journal_title：Journal of bacteriology

authors： Holtzapple E,Schmidt-Dannert C

更新日期：2007-05-01 00:00:00

abstract：:A set of Saccharomyces cerevisiae strains with variable expression of only the high-affinity Hxt7 glucose transporter was constructed by partial deletion of the HXT7 promoter in vitro and integration of the gene at various copy numbers into the genome of an hxt1-7 gal2 deletion strain. The glucose transport capacity i...

journal_title：Journal of bacteriology

authors： Ye L,Kruckeberg AL,Berden JA,van Dam K

更新日期：1999-08-01 00:00:00

abstract：:An understanding of the enzymatic reactions catalyzing the degradation of substituted phenols, a major group of environmental pollutants, is required for the development of biological methods for the decontamination of halophenol-polluted sites. We found that a flavomonooxygenase, pentachlorophenol hydroxylase, isolat...

journal_title：Journal of bacteriology

authors： Xun L,Topp E,Orser CS

更新日期：1992-05-01 00:00:00

abstract：:Enterococcus faecalis ATCC 11700 is able to use arginine and the diamine agmatine as a sole energy source. Via the highly homologous deiminase pathways, arginine and agmatine are converted into CO2, NH3, and the end products ornithine and putrescine, respectively. In the arginine deiminase pathway, uptake of arginine ...

journal_title：Journal of bacteriology

authors： Driessen AJ,Smid EJ,Konings WN

更新日期：1988-10-01 00:00:00

abstract：:The genetic marker for sulfadiazine resistance was transferred by means of purified deoxyribonucleic acid from sulfadiazine-resistant Neisseria meningitidis and N. perflava to a sulfadiazine-sensitive strain of N. meningitidis. Over 80% of the isolates from these experiments, selected on the basis of sulfadiazine resi...

journal_title：Journal of bacteriology

authors： Kingsbury DT

更新日期：1967-09-01 00:00:00