Membrane-bound phosphatases in Escherichia coli: sequence of the pgpA gene.


:One of the phosphatidyl glycerophosphate phosphatase genes of Escherichia coli, pgpA, was cloned, and its DNA sequence was determined. Its 507-base-pair open reading frame was consistent with the 18,000-molecular-weight product identified by a maxicell experiment. Between its possible promoter and methionine initiation codon, a repetitive extragenic palindromic sequence was found.


J Bacteriol


Journal of bacteriology


Icho T




Has Abstract


1988-11-01 00:00:00












  • Capsular polysaccharide production in Enterococcus faecalis and contribution of CpsF to capsule serospecificity.

    abstract::Many bacterial species produce capsular polysaccharides that contribute to pathogenesis through evasion of the host innate immune system. The gram-positive pathogen Enterococcus faecalis was previously reported to produce one of four capsule serotypes (A, B, C, or D). Previous studies describing the four capsule serot...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Thurlow LR,Thomas VC,Hancock LE

    更新日期:2009-10-01 00:00:00

  • Dual response regulators (NarL and NarP) interact with dual sensors (NarX and NarQ) to control nitrate- and nitrite-regulated gene expression in Escherichia coli K-12.

    abstract::Two sensor proteins, NarX and NarQ, mediate nitrate regulation of anaerobic respiratory gene expression. Either of these sensors is sufficient to signal the presence of nitrate to the response regulator protein, NarL, a transcriptional activator and repressor. Two observations suggested the existence of a second respo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Rabin RS,Stewart V

    更新日期:1993-06-01 00:00:00

  • Identification of five new essential genes involved in the synthesis of a secreted protein in Escherichia coli.

    abstract::To define additional components of the export machinery of Escherichia coli, I have isolated extragenic suppressors of a mutant [secA(Ts)] that is temperature sensitive for growth and secretion at 37 degrees C. Suppressors that restored growth at 37 degrees C, but that rendered the cell cold sensitive for growth at 28...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Oliver DB

    更新日期:1985-01-01 00:00:00

  • Analysis of the roles of FlgP and FlgQ in flagellar motility of Campylobacter jejuni.

    abstract::Flagellar motility is an important determinant of Campylobacter jejuni that is required for promoting interactions with various hosts to promote gastroenteritis in humans or commensal colonization of many animals. In a previous study, we identified a nonmotile mutant of C. jejuni 81-176 with a transposon insertion in ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Sommerlad SM,Hendrixson DR

    更新日期:2007-01-01 00:00:00

  • Phosphorylation and functional properties of the IIA domain of the lactose transport protein of Streptococcus thermophilus.

    abstract::The lactose-H+ symport protein (LacS) of Streptococcus thermophilus has a carboxyl-terminal regulatory domain (IIALacS) that is homologous to a family of proteins and protein domains of the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) in various organisms, of which IIAGlc of Escherichia coli is the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Gunnewijk MG,Postma PW,Poolman B

    更新日期:1999-01-01 00:00:00

  • Evidence for multiple terminal oxidases, including cytochrome d, in facultatively alkaliphilic Bacillus firmus OF4.

    abstract::The terminal oxidase content of Bacillus firmus OF4, a facultative alkaliphile that grows well over the pH range of 7.5 to 10.5, was studied by difference spectroscopy. Evidence was found for three terminal oxidases under different growth conditions. The growth pH and the stage of growth profoundly affected the expres...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hicks DB,Plass RJ,Quirk PG

    更新日期:1991-08-01 00:00:00

  • DnaD protein of Bacillus subtilis interacts with DnaA, the initiator protein of replication.

    abstract::The yeast two-hybrid assay revealed that Bacillus subtilis DnaD, a possible component of the primosome and required for replication initiation, interacted with DnaA and DnaD itself. The mutant DnaD23 was incapable of interacting with DnaA but retained interaction with the wild-type DnaD. These results suggest that int...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ishigo-Oka D,Ogasawara N,Moriya S

    更新日期:2001-03-01 00:00:00

  • A novel cdsAB operon is involved in the uptake of L-cysteine and participates in the pathogenesis of Yersinia ruckeri.

    abstract::Application of in vivo expression technology (IVET) to Yersinia ruckeri, an important fish pathogen, allowed the identification of two adjacent genes that represent a novel bacterial system involved in the uptake and degradation of l-cysteine. Analysis of the translational products of both genes showed permease domain...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Méndez J,Reimundo P,Pérez-Pascual D,Navais R,Gómez E,Guijarro JA

    更新日期:2011-02-01 00:00:00

  • Growth kinetics of extremely halophilic archaea (family halobacteriaceae) as revealed by arrhenius plots.

    abstract::Members of the family Halobacteriaceae in the domain Archaea are obligate extreme halophiles. They occupy a variety of hypersaline environments, and their cellular biochemistry functions in a nearly saturated salty milieu. Despite extensive study, a detailed analysis of their growth kinetics is missing. To remedy this...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Robinson JL,Pyzyna B,Atrasz RG,Henderson CA,Morrill KL,Burd AM,Desoucy E,Fogleman RE 3rd,Naylor JB,Steele SM,Elliott DR,Leyva KJ,Shand RF

    更新日期:2005-02-01 00:00:00

  • Reproducible and rapid methods for the isolation and assay of a-factor, a yeast mating hormone.

    abstract::The Saccharomyces cerevisiae mating hormone a-factor has been difficult to isolate reproducibly in sufficient yields by methods using ion-exchange chromatography, probably because of its pronounced hydrophobicity. In this work, a hydrophobic adsorbent (Amberlite XAD-2), in an insoluble bead from, was used to isolate l...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Strazdis JR,MacKay VL

    更新日期:1982-09-01 00:00:00

  • Thickness and elasticity of gram-negative murein sacculi measured by atomic force microscopy.

    abstract::Atomic force microscopy was used to measure the thickness of air-dried, collapsed murein sacculi from Escherichia coli K-12 and Pseudomonas aeruginosa PAO1. Air-dried sacculi from E. coli had a thickness of 3.0 nm, whereas those from P. aeruginosa were 1.5 nm thick. When rehydrated, the sacculi of both bacteria swelle...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Yao X,Jericho M,Pink D,Beveridge T

    更新日期:1999-11-01 00:00:00

  • Synthesis of lipopolysaccharide O side chains by Pseudomonas aeruginosa PAO1 requires the enzyme phosphomannomutase.

    abstract::We have cloned a lipopolysaccharide (LPS) biosynthetic gene from Pseudomonas aeruginosa PAO1 that complements the defect in the production and incorporation of LPS O side chains in the LPS-rough strain AK1012. This gene was characterized by pulsed-field gel electrophoresis, deletion and restriction mapping of the clon...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Goldberg JB,Hatano K,Pier GB

    更新日期:1993-03-01 00:00:00

  • N-Acyl-L-homoserine lactone autoinducers control production of an extracellular lipopeptide biosurfactant required for swarming motility of Serratia liquefaciens MG1.

    abstract::A nonswarming Serratia liquefaciens mutant deficient in serrawettin W2 production was constructed by transposon mutagenesis. Sequence homology indicated that insertion had occurred in gene swrA, which encodes a putative peptide synthetase. Expression of swrA is controlled by quorum sensing. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Lindum PW,Anthoni U,Christophersen C,Eberl L,Molin S,Givskov M

    更新日期:1998-12-01 00:00:00

  • Escherichia coli proteins inducible by oxidative stress mediated by the superoxide radical.

    abstract::Two-dimensional gel analyses were made of proteins synthesized in Escherichia coli during various O2- -generating conditions. Nine proteins were constitutively synthesized over wild-type levels in superoxide dismutase (sodA sodB) double mutants. Addition of redox cycling agents such as paraquat and plumbagin at variou...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Walkup LK,Kogoma T

    更新日期:1989-03-01 00:00:00

  • Effects of m-Tyrosine on Growth and Sporulation of Bacillus Species.

    abstract::Aronson, John N. (Arizona State University, Tempe), and Gerald R. Wermus. Effects of m-tyrosine on growth and sporulation of Bacillus species. J. Bacteriol. 90:38-46. 1965.-The aromatic amino acid analogue, dl-beta-(3-hydroxyphenyl)alanine (m-tyrosine), reduced sporulation of a strain of Bacillus subtilis to less than...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Aronson JN,Wermus GR

    更新日期:1965-07-01 00:00:00

  • Use of a mariner-based transposon mutagenesis system to isolate Clostridium perfringens mutants deficient in gliding motility.

    abstract::Clostridium perfringens is an anaerobic Gram-positive pathogen that causes many human and animal diseases, including food poisoning and gas gangrene. C. perfringens lacks flagella but possesses type IV pili (TFP). We have previously shown that C. perfringens can glide across an agar surface in long filaments composed ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Liu H,Bouillaut L,Sonenshein AL,Melville SB

    更新日期:2013-02-01 00:00:00

  • Metabolism of phospholipid 2-linked fatty acids during the release of membrane fragments from Haemophilus parainfluenzae by ethylenediaminetetraacetic acid-tris(hydroxymethyl)-aminomethane.

    abstract::Membrane fragments containing diacyl phospholipids were released from viable cells of Haemophilus parainfluenzae during incubation in ethylenediaminetetraacetic acid (EDTA)- tris(hydroxymethyl)aminomethane (Tris) buffer. The phospholipids located in the part of the membrane that was released during the EDTA-Tris treat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Tucker AN,White DC

    更新日期:1970-08-01 00:00:00

  • Nucleotide sequence of the gene for the vitamin B12 receptor protein in the outer membrane of Escherichia coli.

    abstract::The nucleotide sequence of a 2220-base-pair fragment containing the btuB gene of Escherichia coli was determined. There was a single open reading frame which was translated into a 614-amino-acid polypeptide, the first 20 amino acids of which comprised a typical leader sequence. The putative mature or processed form ha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Heller K,Kadner RJ

    更新日期:1985-03-01 00:00:00

  • Presence of a novel phosphopentomutase and a 2-deoxyribose 5-phosphate aldolase reveals a metabolic link between pentoses and central carbon metabolism in the hyperthermophilic archaeon Thermococcus kodakaraensis.

    abstract::Numerous bacteria and mammalian cells harbor two enzymes, phosphopentomutase (PPM) and 2-deoxyribose 5-phosphate aldolase (DERA), involved in the interconversion between nucleosides and central carbon metabolism. In this study, we have examined the presence of this metabolic link in the hyperthermophilic archaeon, The...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Rashid N,Imanaka H,Fukui T,Atomi H,Imanaka T

    更新日期:2004-07-01 00:00:00

  • Effect of temperature on Pseudomonas fluorescens chemotaxis.

    abstract::The effects of temperature and attractants on chemotaxis in psychrotrophic Pseudomonas fluorescens were examined using the Adler capillary assay technique. Several organic acids, amino acids, and uronic acids were shown to be attractants, whereas glucose and its oxidation products, gluconate and 2-ketogluconate, elici...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Lynch WH

    更新日期:1980-07-01 00:00:00

  • Citrate synthase mutants of Sinorhizobium meliloti are ineffective and have altered cell surface polysaccharides.

    abstract::The gltA gene, encoding Sinorhizobium meliloti 104A14 citrate synthase, was isolated by complementing an Escherichia coli gltA mutant. The S. meliloti gltA gene was mutated by inserting a kanamycin resistance gene and then using homologous recombination to replace the wild-type gltA with the gltA::kan allele. The resu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Mortimer MW,McDermott TR,York GM,Walker GC,Kahn ML

    更新日期:1999-12-01 00:00:00

  • phs Locus of Escherichia coli, a mutation causing pleiotropic lesions in metabolism, is an rpoA allele.

    abstract::The phs mutation, which causes a pleiotropic growth defect, has been mapped and shown to be an allele of rpoA, the gene for the alpha subunit of RNA polymerase. The mutation is shown to cause a transcription defect in the arabinose operon, araBAD. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Rowland GC,Giffard PM,Booth IR

    更新日期:1985-11-01 00:00:00

  • Genetic and physical analyses of Caulobacter crescentus trp genes.

    abstract::Caulobacter crescentus trp mutants were identified from a collection of auxotrophs. Precursor feeding experiments, accumulation studies, and complementation experiments resulted in the identification of six genes corresponding to trpA, trpB, trpC, trpD, trpE, and trpF. Genetic mapping experiments demonstrated that the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Winkler ME,Schoenlein PV,Ross CM,Barrett JT,Ely B

    更新日期:1984-10-01 00:00:00

  • In vitro sulfotransferase activity of NodH, a nodulation protein of Rhizobium meliloti required for host-specific nodulation.

    abstract::Early stages of nodulation involve the exchange of signals between the bacterium and the host plant. Bacterial nodulation (nod) genes are required for Rhizobium spp. to synthesize lipooligosaccharide morphogens, termed Nod factors. The common nod genes encode enzymes that synthesize the factor core structure, which is...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Ehrhardt DW,Atkinson EM,Faull KF,Freedberg DI,Sutherlin DP,Armstrong R,Long SR

    更新日期:1995-11-01 00:00:00

  • Modifiable chromatophore proteins in photosynthetic bacteria.

    abstract::The chromatophores of Chromatium vinosum, as well as six other photosynthetic bacteria, contained two or more proteins which were insoluble when heated in the presence of sodium dodecyl sulfate (SDS) and 2-mercaptoethanol (beta-ME). When the chromatophores were dissolved at room temperature in SDS-beta-ME, these prote...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Hui KM,Hurlbert RE

    更新日期:1979-04-01 00:00:00

  • Regulation of a phage endolysin by disulfide caging.

    abstract::In contrast to canonical phage endolysins, which require holin-mediated disruption of the membrane to gain access to attack the cell wall, signal anchor release (SAR) endolysins are secreted by the host sec system, where they accumulate in an inactive form tethered to the membrane by their N-terminal SAR domains. SAR ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Kuty GF,Xu M,Struck DK,Summer EJ,Young R

    更新日期:2010-11-01 00:00:00

  • Presence of multiple sites containing polar material in spherical Escherichia coli cells that lack MreB.

    abstract::In rod-shaped bacteria, certain proteins are specifically localized to the cell poles. The nature of the positional information that leads to the proper localization of these proteins is unclear. In a screen for factors required for the localization of the Shigella sp. actin assembly protein IcsA to the bacterial pole...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Nilsen T,Yan AW,Gale G,Goldberg MB

    更新日期:2005-09-01 00:00:00

  • Ribonucleic acid and protein synthesis in Rhizophlyctis rosea zoospores.

    abstract::LéJohn, Herbert B. (Purdue University, Lafayette, Ind.), and James S. Lovett. Ribonucleic acid and protein synthesis in Rhizophlyctis rosea zoospores. J. Bacteriol. 91:709-717. 1966.-The uniflagellate zoospores of Rhizophlyctis rosea display active motility and a high endogenous respiratory metabolism, but neither gro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Léjohn HB,Lovett JS

    更新日期:1966-02-01 00:00:00

  • Production of an endo-beta-N-acetylglucosaminidase activity mediates growth of Enterococcus faecalis on a high-mannose-type glycoprotein.

    abstract::Enterococcus faecalis is associated with a high proportion of nosocomial infections; however, little is known of the ability of this organism to proliferate in vivo. The ability of RNase B, a model glycoprotein with a single N-glycosylation site occupied by a family of high-mannose-type glycans (Man(5)- to Man(9)-GlcN...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Roberts G,Tarelli E,Homer KA,Philpott-Howard J,Beighton D

    更新日期:2000-02-01 00:00:00

  • Effect of netropsin on the derepression of enzymes during growth and sporulation of Bacillus subtilis.

    abstract::Netropsin, a polypeptide antibiotic which binds specifically to adenylate-thymidylate-rich regions of deoxyribonucleic acid, inhibitis sporulation at about stage II, but does not inhibit growth of Bacillys subtilis. An analysis of the sporulation-associated enzymes aconitase, alkaline phosphatase, and glucose dehydrog...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章


    authors: Keilman GR,Brutis K,Tanimoto B,Doi RH

    更新日期:1976-10-01 00:00:00