Membrane-bound phosphatases in Escherichia coli: sequence of the pgpA gene.

abstract：:Pseudomonas aeruginosa is usually resistant to a wide variety of antibacterial agents, and it has been inferred, on the basis of indirect evidence, that this was due to the low permeability of its outer membrane. We determined the permeability of P. aeruginosa outer membrane directly, by measuring the rates of hydroly...

journal_title：Journal of bacteriology

authors： Yoshimura F,Nikaido H

更新日期：1982-11-01 00:00:00

abstract：:The gal3 mutation of Saccharomyces, which is associated with an impairment in the utilization of galactose, has been shown to be pleiotropic, causing similar impairments in the utilization of melibiose and maltose. Milibiose utilization and alpha-galactosidase production are directly controlled by the galactose regula...

journal_title：Journal of bacteriology

authors： Kew OM,Douglas HC

更新日期：1976-01-01 00:00:00

abstract：:The nonstarter lactic acid bacterium Lactococcus raffinolactis is prevalent in a wide range of environments, such as the dairy environment, but little is known about this species. Here, we present the draft genome of Lactococcus raffinolactis strain 4877, isolated from a natural mesophilic dairy starter culture. ...

journal_title：Journal of bacteriology

authors： Meslier V,Loux V,Renault P

更新日期：2012-11-01 00:00:00

abstract：:Molecular phylogenetic surveys have recently revealed an ecologically widespread crenarchaeal group that inhabits cold and temperate terrestrial and marine environments. To date these organisms have resisted isolation in pure culture, and so their phenotypic and genotypic characteristics remain largely unknown. To cha...

journal_title：Journal of bacteriology

authors： Schleper C,DeLong EF,Preston CM,Feldman RA,Wu KY,Swanson RV

更新日期：1998-10-01 00:00:00

abstract：:A novel mutant of the marine oil-degrading bacterium Alcanivorax borkumensis SK2, containing a mini-Tn5 transposon disrupting a "tesB-like" acyl-coenzyme A (CoA) thioesterase gene, was found to hyperproduce polyhydroxyalkanoates (PHA), resulting in the extracellular deposition of this biotechnologically important poly...

journal_title：Journal of bacteriology

authors： Sabirova JS,Ferrer M,Lünsdorf H,Wray V,Kalscheuer R,Steinbüchel A,Timmis KN,Golyshin PN

更新日期：2006-12-01 00:00:00

abstract：:PB15 is an Hfr strain of Escherichia coli K-12. It arose from an F' strain carrying a temperature-sensitive F-gal by an event which blocked the detachment of F-gal in the normally reversible integration process. In PB15, the detachment of F-gal by a second mechanism can now be detected: this mechanism results in the e...

journal_title：Journal of bacteriology

authors： Bergquist PL,Adelberg EA

更新日期：1972-07-01 00:00:00

abstract：:The Escherichia coli dnaN159 allele encodes a mutant form of the beta-sliding clamp (beta159) that is impaired for interaction with the replicative DNA polymerase (Pol), Pol III. In addition, strains bearing the dnaN159 allele require functional Pol I for viability. We have utilized a combination of genetic and bioche...

journal_title：Journal of bacteriology

authors： Maul RW,Sanders LH,Lim JB,Benitez R,Sutton MD

更新日期：2007-07-01 00:00:00

abstract：:The recent discovery that the protein DksA acts as a coregulator of genes controlled by ppGpp led us to investigate the similarities and differences between the relaxed phenotype of a ppGpp-deficient mutant and the phenotype of a strain lacking DksA. We demonstrate that the absence of DksA and ppGpp has similar effect...

journal_title：Journal of bacteriology

authors： Magnusson LU,Gummesson B,Joksimović P,Farewell A,Nyström T

更新日期：2007-07-01 00:00:00

abstract：:In the photosynthetic bacterium Rhodobacter capsulatus, the enzyme DNA gyrase has been implicated in the expression of genes for anaerobic metabolic processes such as nitrogen fixation and photosynthesis. To assess the involvement of supercoiling in anaerobic gene expression, we have developed an assay to detect in vi...

journal_title：Journal of bacteriology

authors： Cook DN,Armstrong GA,Hearst JE

更新日期：1989-09-01 00:00:00

abstract：:The pdxK and pdxY genes have been found to code for pyridoxal kinases, enzymes involved in the pyridoxal phosphate salvage pathway. Two pyridoxal kinase structures have recently been published, including Escherichia coli pyridoxal kinase 2 (ePL kinase 2) and sheep pyridoxal kinase, products of the pdxY and pdxK genes,...

journal_title：Journal of bacteriology

authors： Safo MK,Musayev FN,di Salvo ML,Hunt S,Claude JB,Schirch V

更新日期：2006-06-01 00:00:00

abstract：:Maleylacetate reductase (EC 1.3.1.32) plays a major role in the degradation of chloroaromatic compounds by channelling maleylacetate and some chlorinated derivatives into the 3-oxoadipate pathway. Several substituted maleylacetates were prepared in situ by alkaline or enzymatic hydrolysis of dienelactones as the precu...

journal_title：Journal of bacteriology

authors： Kaschabek SR,Reineke W

更新日期：1995-01-01 00:00:00

abstract：:Escherichia coli acetyl coenzyme A carboxylase (ACC), the first enzyme of the fatty acid biosynthetic pathway, is inhibited by acylated derivatives of acyl carrier protein (ACP). ACP lacking an acyl moiety does not inhibit ACC. Acylated derivatives of ACP having chain lengths of 6 to 20 carbon atoms were similarly inh...

journal_title：Journal of bacteriology

authors： Davis MS,Cronan JE Jr

更新日期：2001-02-01 00:00:00

abstract：:We studied the following two aspects of the glucose effect on galactose operon expression in Escherichia coli K-12: catabolite repression and inducer exclusion. Using both inducible and constitutive strains and measuring the rate of promoter-proximal enzyme synthesis, we found that the galactose operon did not seem to...

journal_title：Journal of bacteriology

authors： Joseph E,Danchin A,Ullmann A

更新日期：1981-04-01 00:00:00

abstract：:Addition of casein hydrolysate to suspensions of washed, nonpigmented, nonproliferating Serratia marcescens incubating at 27 C induced biosynthesis of prodigiosin. Four amino acids of casein hydrolysate, dl-aspartic acid, l-glutamic acid, l-proline, and l-alanine caused formation of pigment when added individually. dl...

journal_title：Journal of bacteriology

authors： Williams RP,Gott CL,Qadri SM

更新日期：1971-05-01 00:00:00

abstract：:The type III secretion (T3S) system is essential to the virulence of a large number of Gram-negative bacterial pathogens, including Yersinia. YscO is required for T3S in Yersinia and is known to interact with several other T3S proteins, including the chaperone SycD and the needle length regulator YscP. To define which...

journal_title：Journal of bacteriology

authors： Mukerjea R,Ghosh P

更新日期：2013-10-01 00:00:00

abstract：:A three-dimensional tracking microscope was used to quantify the effects of temperature (50 to 80 degrees C) and pH (2 to 4) on the motility of Sulfolobus acidocaldarius, a thermoacidophilic archaeon. Swimming speed and run time increased with temperature but remained relatively unchanged with increasing pH. These res...

journal_title：Journal of bacteriology

authors： Lewus P,Ford RM

更新日期：1999-07-01 00:00:00

abstract：:The major acid-soluble spore proteins (ASSPs) of Bacillus subtilis were detected by immunoprecipitation of radioactively labeled in vitro- and in vivo-synthesized proteins. ASSP synthesis in vivo began 2 h after the initiation of sporulation (t2) and reached its maximum rate at t7. This corresponded to the time of syn...

journal_title：Journal of bacteriology

authors： Leventhal JM,Chambliss GH

更新日期：1982-12-01 00:00:00

abstract：:Saccharomyces cerevisiae cells, grown on a fermentable carbon source, display two growth phases before they enter the stationary phase: a rapid phase (log phase) followed by a slow phase. It was previously shown that a subunit of the yeast RNA polymerase II, RPB4, positively affects the activity of the enzyme in post-...

journal_title：Journal of bacteriology

authors： Choder M

更新日期：1993-10-01 00:00:00

abstract：:MinD binds to phospholipid vesicles in the presence of ATP and is released by MinE, which stimulates the MinD ATPase. Membrane binding requires a short conserved C-terminal region, which has the potential to form an amphipathic helix. This finding has led to a model in which the binding of ATP regulates the formation ...

journal_title：Journal of bacteriology

authors： Zhou H,Lutkenhaus J

更新日期：2003-08-01 00:00:00

abstract：:Unsaturated fatty acids having structural features which are different from those of the monoenoic acids normally synthesized by Escherichia coli can serve as growth factors for an auxotroph requiring unsaturated fatty acids. These analogues were incorporated into the phospholipids, as shown by gas-liquid and thin-lay...

journal_title：Journal of bacteriology

authors： Silbert DF,Ruch F,Vagelos PR

更新日期：1968-05-01 00:00:00

abstract：:The mutB gene of Salmonella typhimurium is involved in a methylation-independent repair pathway specific for A/G or A/C mismatches and is the homolog of the Escherichia coli mutY gene. The mutB gene of S. typhimurium was cloned and sequenced. The isolated mutB clone reduced the mutation rate of the mutB mutant to wild...

journal_title：Journal of bacteriology

authors： Desiraju V,Shanabruch WG,Lu AL

更新日期：1993-01-01 00:00:00

abstract：:Expression of toxT, the transcription activator of cholera toxin and pilus production in Vibrio cholerae, is the consequence of a complex cascade of regulatory events that culminates in activation of the toxT promoter by TcpP and ToxR, two membrane-localized transcription factors. Both are encoded in operons with gene...

journal_title：Journal of bacteriology

authors： Beck NA,Krukonis ES,DiRita VJ

更新日期：2004-12-01 00:00:00

abstract：:Late log-phase Escherichia coli B/r cells are 1.6 times more sensitive to killing by X rays than are stationary-phase cells when grown in Brain Heart Infusion (BHI) + glucose. The number of single-chain breaks formed per krad is the same for log- and stationary-phase cells. Stationary-phase cells show a somewhat great...

journal_title：Journal of bacteriology

authors： Town CD,Smith KC,Kaplan HS

更新日期：1971-01-01 00:00:00

abstract：:Diacyl phthiocerol esters and their congeners are mycobacterial virulence factors. The biosynthesis of these complex lipids remains poorly understood. Insight into their biosynthesis will aid the development of rationally designed drugs that inhibit their production. In this study, we investigate a biosynthetic step r...

journal_title：Journal of bacteriology

authors： Onwueme KC,Vos CJ,Zurita J,Soll CE,Quadri LE

更新日期：2005-07-01 00:00:00

abstract：:Oxygen utilization in splenic cells of normal and BCG-sensitized guinea pigs was measured in a Warburg respirometer in the absence and in the presence of Old Tuberculin (OT). In the absence of OT, normal and sensitive cells respired at the same rate; in the presence of OT, normal cell respiration remained unchanged, w...

journal_title：Journal of bacteriology

authors： Kochan I,Khan A

更新日期：1969-01-01 00:00:00

abstract：:We identified several promoters responsible for the expression of regA, which encodes a global virulence regulator in Citrobacter rodentium. Expression of some of the promoters was strongly autoactivated by RegA in conjunction with bicarbonate. Biochemical and mutational analyses were used to determine the consensus s...

journal_title：Journal of bacteriology

authors： Tan A,Yang J,Tauschek M,Praszkier J,Robins-Browne RM

更新日期：2011-04-01 00:00:00

abstract：:Previous determination of the nucleotide sequence of the iol region of the Bacillus subtilis genome allowed us to predict the structure of the iol operon for myo-inositol catabolism, consisting of 10 iol genes (iolA to iouJ); iolG corresponds to idh, encoding myo-inositol 2-dehydrogenase (Idh). Primer extension analys...

journal_title：Journal of bacteriology

authors： Yoshida KI,Aoyama D,Ishio I,Shibayama T,Fujita Y

更新日期：1997-07-01 00:00:00

abstract：:The genetic transformation mutant Rd(DB117)rec- has a pleiotropic phenotype that includes reduced levels of phage recombination. Physical mapping experiments showed that this strain has a 78.5-kbp insertion in the rec-2 gene. The rec-2 dependence of phage recombination was reexamined to determine whether the defective...

journal_title：Journal of bacteriology

authors： Kupfer DM,McCarthy D

更新日期：1992-08-01 00:00:00

abstract：:A primary determinant of pathogenicity in Erwinia stewartii is the production of extracellular polysaccharide (EPS). A single mutation can abolish both EPS synthesis and pathogenicity; both properties are restored by a single cosmid clone. Subcloning and insertion analysis have defined a single positive regulatory fun...

journal_title：Journal of bacteriology

authors： Torres-Cabassa A,Gottesman S,Frederick RD,Dolph PJ,Coplin DL

更新日期：1987-10-01 00:00:00

abstract：:With two-dimensional restriction enzyme analysis we have been able to cleave the Bacillus subtilis genome and resolve the resulting deoxyribonucleic acid (DNA) segments into discrete bands on agarose gels. A general procedure for gene purification has been developed by coupling multidimensional restriction analysis wi...

journal_title：Journal of bacteriology

authors： Potter SS,Bott KF,Newbold JE

更新日期：1977-01-01 00:00:00