Genomic analysis reveals chromosomal variation in natural populations of the uncultured psychrophilic archaeon Cenarchaeum symbiosum.

abstract：:HypA and HypB are maturation proteins required for incorporation of nickel into the hydrogenase large subunit. To examine the functions of these proteins in nickel insertion, the hybF gene, which is a homolog of hypA essential for maturation of hydrogenases 1 and 2 from Escherichia coli, was overexpressed, and the pro...

journal_title：Journal of bacteriology

authors： Blokesch M,Rohrmoser M,Rode S,Böck A

更新日期：2004-05-01 00:00:00

abstract：:Massetolide A is a cyclic lipopeptide (CLP) antibiotic produced by various Pseudomonas strains from diverse environments. Cloning, sequencing, site-directed mutagenesis, and complementation showed that massetolide A biosynthesis in P. fluorescens SS101 is governed by three nonribosomal peptide synthetase (NRPS) genes,...

journal_title：Journal of bacteriology

authors： de Bruijn I,de Kock MJ,de Waard P,van Beek TA,Raaijmakers JM

更新日期：2008-04-01 00:00:00

abstract：:The organization and transcriptional control of chromosomal cat genes (required for dissimilation of catechol by the beta-ketoadipate pathway) in the Pseudomonas putida biotype strain (ATCC 12633) are reported. Nucleotide sequence reveals that catR is separated by 135 bp from the divergently transcribed catBC,A; catC ...

journal_title：Journal of bacteriology

authors： Houghton JE,Brown TM,Appel AJ,Hughes EJ,Ornston LN

更新日期：1995-01-01 00:00:00

abstract：:Microbacterium yannicii is a Gram-positive, aerobic, yellow-pigmented, rod-shaped, nonmotile, oxidase-negative, and catalase-positive bacterium isolated on Columbia colistin-nalidixic acid (CNA) agar with 5% sheep blood from the sputum of a cystic fibrosis patient. The present study reports the draft genome of a Micro...

journal_title：Journal of bacteriology

authors： Sharma P,Diene SM,Gimenez G,Robert C,Rolain JM

更新日期：2012-09-01 00:00:00

abstract：:The Put3p and Gal4p transcriptional activators are members of a distinct class of fungal regulators called the Cys(6) Zn(II)(2) binuclear cluster family. This family includes over 50 different Saccharomyces cerevisiae proteins that share a similar domain organization. Gal4p activates the genes of the galactose utiliza...

journal_title：Journal of bacteriology

authors： D'Alessio M,Brandriss MC

更新日期：2000-07-01 00:00:00

abstract：:A series of R (rough) Salmonella minnesota mutants with rfb, rfe, and rfa mutations leading to various defects in the biosynthesis of cell wall lipopolysaccharide was analyzed as to their enterobacterial common antigen (CA) content. All mutants that had functional rfe genes were CA(+) as is the wild-type parent. This ...

journal_title：Journal of bacteriology

authors： Mäkelä PH,Mayer H,Whang HY,Neter E

更新日期：1974-09-01 00:00:00

abstract：:Mu promoters active during the lytic cycle were located by isolating RNA at various times after induction of Mu prophages, radiolabeling it by capping in vitro, and hybridizing it to Mu DNA fragments on Southern blots. Signals were detected from four new promoters in addition to the previously characterized Pe (early)...

journal_title：Journal of bacteriology

authors： Stoddard SF,Howe MM

更新日期：1989-06-01 00:00:00

abstract：:Tn2424, a multiresistance transposon 25 kilobases long, was isolated from IncFII plasmid NR79. Tn2424 transposed resistance to sulfonamides, streptomycin and spectinomycin, mercuric chloride, chloramphenicol, and amikacin with a frequency of 6 X 10(-5). Resistance to amikacin was mediated by a 6'-N-acetyltransferase, ...

journal_title：Journal of bacteriology

authors： Meyer JF,Nies BA,Wiedemann B

更新日期：1983-08-01 00:00:00

abstract：:We have cloned the Micromonospora viridifaciens neuraminidase (EC 3.2.1.18) gene (nedA) in Streptomyces lividans. This was accomplished by using the vector pIJ702 and BglII-BclI libraries of M. viridifaciens chromosomal inserts created in S. lividans. The libraries were screened for the expression of neuraminidase by ...

journal_title：Journal of bacteriology

authors： Sakurada K,Ohta T,Hasegawa M

更新日期：1992-11-01 00:00:00

abstract：:The production and regeneration of bacterial protoplasts promoted the loss of three different plasmid-specified traits in Streptococcus lactis subsp. diacetylactis strains. The loss of five different plasmids, including small multicopy molecules, was readily detected in Streptococcus lactis 712 by screening lysates of...

journal_title：Journal of bacteriology

authors： Gasson MJ

更新日期：1983-04-01 00:00:00

abstract：:The effects of competence-inducing treatments on the composition and organization of membrane lipids in Escherichia coli K-12, DH1, DH5, HB101, and RR1 were investigated for two widely used protocols in which transformability is developed at low temperatures in Ca2+ buffers. At stages during each procedure, the lipid ...

journal_title：Journal of bacteriology

authors： Reusch RN,Hiske TW,Sadoff HL

更新日期：1986-11-01 00:00:00

abstract：:Gene sequences encoding the enzymes UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) from many bacterial sources were analyzed. It was shown that whereas gram-negative bacteria have only one murA gene, gram-positive bacteria have two distinct genes encoding these enzymes which have possibly arisen from gene dupl...

journal_title：Journal of bacteriology

authors： Du W,Brown JR,Sylvester DR,Huang J,Chalker AF,So CY,Holmes DJ,Payne DJ,Wallis NG

更新日期：2000-08-01 00:00:00

abstract：:Bacillus subtilis responds to phosphate starvation stress by inducing the PhoP and SigB regulons. While the PhoP regulon provides a specific response to phosphate starvation stress, maximizing the acquisition of phosphate (P(i)) from the environment and reducing the cellular requirement for this essential nutrient, th...

journal_title：Journal of bacteriology

authors： Allenby NE,O'Connor N,Prágai Z,Ward AC,Wipat A,Harwood CR

更新日期：2005-12-01 00:00:00

abstract：:Ishibashi, Masahide (Osaka University, Osaka, Japan), Yoshinobu Sugino, and Yukinori Hirota. Chromosomal location of thymine and arginine genes in Escherichia coli and an F' incorporating them. J. Bacteriol. 87:554-561. 1964.-The gene responsible for thymine requirement or independence in Escherichia coli K-12 is loca...

journal_title：Journal of bacteriology

authors： ISHIBASHI M,SUGINO Y,HIROTA Y

更新日期：1964-03-01 00:00:00

abstract：:In wild-type Escherichia coli the activity of the maltose transport system is dependent on a periplasmic maltose-binding protein. It has been possible, however, to isolate mutants in which transport activity is mediated by the membrane components of the system and is no longer dependent on the periplasmic binding prot...

journal_title：Journal of bacteriology

authors： Reyes M,Treptow NA,Shuman HA

更新日期：1986-03-01 00:00:00

abstract：:Spore formation of Myxococcus xanthus can occur not only on agar plates during fruiting body formation, but also in a liquid culture by simply adding glycerol, dimethyl sulfoxide, or phenethyl alcohol to the culture. This chemically-induced spore formation occurs synchronously and much faster than that occurring durin...

journal_title：Journal of bacteriology

authors： Komano T,Inouye S,Inouye M

更新日期：1980-12-01 00:00:00

abstract：:The genetic marker for sulfadiazine resistance was transferred by means of purified deoxyribonucleic acid from sulfadiazine-resistant Neisseria meningitidis and N. perflava to a sulfadiazine-sensitive strain of N. meningitidis. Over 80% of the isolates from these experiments, selected on the basis of sulfadiazine resi...

journal_title：Journal of bacteriology

authors： Kingsbury DT

更新日期：1967-09-01 00:00:00

abstract：:Clostridium difficile is the etiological agent of antibiotic-associated diarrhea. Among the factors that may play a role in infection are S-layer proteins (SLPs). Previous work has shown these to consist mainly of two components, resulting from the cleavage of a precursor encoded by the slpA gene. The high-molecular-w...

journal_title：Journal of bacteriology

authors： Calabi E,Fairweather N

更新日期：2002-07-01 00:00:00

abstract：:The genes encoding the rrn operons, the 4.5S and 6S RNAs, elements of protein secretion, and outer membrane proteins F and I, and regulatory as well as structural genes for exotoxin A, alkaline phosphatase, and alginate and tryptophan biosynthesis, were assigned on the SpeI/DpnI macrorestriction map of the Pseudomonas...

journal_title：Journal of bacteriology

authors： Römling U,Duchéne M,Essar DW,Galloway D,Guidi-Rontani C,Hill D,Lazdunski A,Miller RV,Schleifer KH,Smith DW

更新日期：1992-01-01 00:00:00

abstract：:A 3.6-kb EcoRI fragment containing the ntrA gene of Agrobacterium tumefaciens was cloned by using the homologous ntrA gene of Rhizobium meliloti as a probe. Construction of an ntrA mutant of A. tumefaciens by site-directed insertional mutagenesis demonstrated the requirement of the ntrA gene for nitrate utilization an...

journal_title：Journal of bacteriology

authors： Wu ZL,Charles TC,Wang H,Nester EW

更新日期：1992-04-01 00:00:00

abstract：:A chromosomal insertion of transposon Tn917 partially restores the expression of protease and alpha-toxin activities to PM466, a genetically defined agr-null derivative of the wild-type Staphylococcus aureus strain RN6390. In co-transduction experiments, transposon-encoded erythromycin resistance and a protease- and a...

journal_title：Journal of bacteriology

authors： McNamara PJ,Milligan-Monroe KC,Khalili S,Proctor RA

更新日期：2000-06-01 00:00:00

abstract：:Bacteriocin 28b from Serratia marcescens binds to Escherichia coli outer membrane proteins OmpA and OmpF and to lipopolysaccharide (LPS) core (J. Enfedaque, S. Ferrer, J. F. Guasch, J. Tomás, and M. Requé, Can. J. Microbiol. 42:19-26, 1996). A cosmid-based genomic library of S. marcescens was introduced into E. coli N...

journal_title：Journal of bacteriology

authors： Guasch JF,Piqué N,Climent N,Ferrer S,Merino S,Rubires X,Tomas JM,Regué M

更新日期：1996-10-01 00:00:00

abstract：:Starvation of Escherichia coli for potassium, phosphate, or magnesium ions leads to a reversible increase in the rate of protein degradation and an inhibition of ribonucleic acid (RNA) synthesis. In cells deprived of potassium, the breakdown of the more stable cell proteins increased two- to threefold, whereas the hyd...

journal_title：Journal of bacteriology

authors： St John AC,Goldberg AL

更新日期：1980-09-01 00:00:00

abstract：:Genetic studies have shown that the 53-kDa (Exo53) and 49-kDa (ExoS) forms of exoenzyme S of Pseudomonas aeruginosa are encoded by separate genes, termed exoT and exoS, respectively. Although ExoS and Exo53 possess 76% primary amino acid homology, Exo53 has been shown to express ADP-ribosyltransferase activity at abou...

journal_title：Journal of bacteriology

authors： Liu S,Yahr TL,Frank DW,Barbieri JT

更新日期：1997-03-01 00:00:00

abstract：:Two inhibitors of replicative deoxyribonucleic acid (DNA) synthesis, nalidixic acid (NAL) and 6-(p-hydroxyphenylazo)-uracil (HPUra), showed different effects on genetic recombination and DNA repair in Bacillus subtilis. Previous work (Pedrini et al., 1972) showed that NAL does not interfere with the transformation pro...

journal_title：Journal of bacteriology

authors： Canosi U,Siccardi AG,Falaschi A,Mazza G

更新日期：1976-04-01 00:00:00

abstract：:A second synchrony induction treatment, half-way through the deoxyribonucleic acid replication process of an already synchronized culture, resulted in defective subsequent cell growth. This supports a previous explanation for the partial loss of viability after synchrony induction. ...

journal_title：Journal of bacteriology

authors： Herdman M,Carr NG

更新日期：1971-08-01 00:00:00

abstract：:Rhizobium sp. strain NGR234 produces a large family of lipochitooligosaccharide Nod factors carrying specific substituents. Among them are 3-O- (or 4-O-) and 6-O-carbamoyl groups, an N-methyl group, and a 2-O-methylfucose residue which may bear either 3-O-sulfate or 4-O-acetyl substitutions. Investigations on the gene...

journal_title：Journal of bacteriology

authors： Quesada-Vincens D,Fellay R,Nasim T,Viprey V,Burger U,Prome JC,Broughton WJ,Jabbouri S

更新日期：1997-08-01 00:00:00

abstract：:We have cloned a DNA fragment from a genomic library of Myxococcus xanthus using an oligonucleotide probe representing conserved regions of biotin carboxylase subunits of acetyl coenzyme A (acetyl-CoA) carboxylases. The fragment contained two open reading frames (ORF1 and ORF2), designated the accB and accA genes, cap...

journal_title：Journal of bacteriology

authors： Kimura Y,Miyake R,Tokumasu Y,Sato M

更新日期：2000-10-01 00:00:00

abstract：:The origin of replication of the plasmid R1162 contains an initiation site for the synthesis of each DNA strand. When one of these sites (oriL) is deleted, synthesis on the corresponding strand is no longer initiated efficiently in vitro by the R1162-encoded replication proteins, and the plasmid is no longer stably ma...

journal_title：Journal of bacteriology

authors： Becker EC,Zhou H,Meyer RJ

更新日期：1996-08-01 00:00:00

abstract：:The polar pili of Pseudomonas aeruginosa are composed of subunits encoded by the pilA gene. Expression of pilA requires the alternative sigma factor RpoN and a pair of regulatory elements, PilS and PilR. These two proteins are members of the two-component regulatory family, in which PilS is the sensory component and P...

journal_title：Journal of bacteriology

authors： Boyd JM,Lory S

更新日期：1996-02-01 00:00:00