Plasmid complements of Streptococcus lactis NCDO 712 and other lactic streptococci after protoplast-induced curing.

Abstract:

:The production and regeneration of bacterial protoplasts promoted the loss of three different plasmid-specified traits in Streptococcus lactis subsp. diacetylactis strains. The loss of five different plasmids, including small multicopy molecules, was readily detected in Streptococcus lactis 712 by screening lysates of random protoplast regenerants on agarose gels. In this strain sequential rounds of protoplast regeneration were used to produce a plasmid-free strain and derivatives carrying only single molecules from the plasmid complement. During these experiments a 33-megadalton plasmid, pLP712, was found to encode genes for lactose and protein utilization. Only this plasmid was required for normal growth and acid production in milk; the remaining four plasmids appeared to be cryptic. Lactose-defective derivatives of a strain carrying only pLP712 were readily isolated. Although these derivatives included instances of plasmid loss, deletions of pLP712 were frequently found. Many different deleted derivatives of pLP712, including some in which the lactose or protein utilization determinant or both were lost, were isolated. The molecular instability of pLP712 largely accounted for previous observations of plasmid complements in S. lactis 712 after lactose determinant curing or transfer by conjugation and transduction. Curing of cryptic molecules from multiple plasmid complements by protoplast regeneration may prove to be generally valuable in lactic streptococci and other gram-positive species.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Gasson MJ

doi

10.1128/JB.154.1.1-9.1983

subject

Has Abstract

pub_date

1983-04-01 00:00:00

pages

1-9

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

154

pub_type

杂志文章
  • Tyrosine and phenylalanine biosynthesis in Escherichia coli K-12: complementation between different tyrR alleles.

    abstract::Dominance tests in diploids have confirmed that the product of the tyrR gene is involved in a negative control system affecting the synthesis of both 3-deoxy-d-arabinoheptulosonic acid 7-phosphate (DAHP) synthetase (tyr) and DAHP synthetase (phe). Some tyrR mutants are derepressed for the synthesis of both DAHP synthe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.115.3.1145-1150.1973

    authors: Im SW,Pittard J

    更新日期:1973-09-01 00:00:00

  • Deoxyribonucleic acid synthesis in Saccharomyces cerevisiae cells permeabilized with ether.

    abstract::Cells of Saccharomyces cerevisiae permeabilized by treatment with ether take up and incorporate exogenous deoxynucleoside triphosphate into deoxyribonucleic acid (DNA). With rho(+) strains, more than 95% of the product was mitochondrial DNA (mtDNA). This report characterizes ether-permeabilized yeast cells and describ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.140.2.333-341.1979

    authors: Oertel W,Goulian M

    更新日期:1979-11-01 00:00:00

  • RflM functions as a transcriptional repressor in the autogenous control of the Salmonella Flagellar master operon flhDC.

    abstract::Motility of bacteria like Salmonella enterica is a highly regulated process that responds to a variety of internal and external stimuli. A hierarchy of three promoter classes characterizes the Salmonella flagellar system, and the onset of flagellar gene expression depends on the oligomeric regulatory complex and class...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00728-13

    authors: Singer HM,Erhardt M,Hughes KT

    更新日期:2013-09-01 00:00:00

  • IDENTIFICATION OF THE STRUCTURAL GENE FOR BETA-GLUCOSIDASE IN SACCHAROMYCES LACTIS.

    abstract::Herman, Alberta (University of Wisconsin, Madison) and Harlyn Halvorson. Identification of the structural gene for beta-glucosidase in Saccharomyces lactis. J. Bacteriol. 85:895-900. 1963.-Three allelic forms (B(h, m, l)) of the structural gene for beta-glucosidase have been identified in the yeast Saccharomyces lacti...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.85.4.895-900.1963

    authors: HERMAN A,HALVORSON H

    更新日期:1963-04-01 00:00:00

  • Role of the catabolite activator protein in the maltose regulon of Escherichia coli.

    abstract::The maltose regulon consists of three operons controlled by a positive regulatory gene, malT. Deletions of the gene crp were introduced into strains which carried a malT-lacZ hybrid gene. From the observed reduction in beta-galactosidase activity it was concluded that the expression of malT-lacZ, and therefore of malT...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.150.2.722-729.1982

    authors: Chapon C

    更新日期:1982-05-01 00:00:00

  • Rapid identification of bacterial genes that are lethal when cloned on multicopy plasmids.

    abstract::A procedure to identify genes that are lethal when cloned on multicopy plasmids was developed. It depends on the ability of mini-Mu plasmid elements to be used for both in vivo cloning and generalized transduction of enterobacterial genes. The feasibility of this procedure was demonstrated by using the tetA gene of Tn...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.1.468-470.1988

    authors: Berg CM,Liu L,Wang B,Wang MD

    更新日期:1988-01-01 00:00:00

  • A priA Mutant Expressed in Two Pieces Has Almost Full Activity in Escherichia coli K-12.

    abstract::The ability to restart broken DNA replication forks is essential across all domains of life. In Escherichia coli, the priA, priB, priC, and dnaT genes encode the replication restart proteins (RRPs) to accomplish this task. PriA plays a critical role in replication restart such that its absence reveals a dramatic pheno...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00267-17

    authors: Leroux M,Jani N,Sandler SJ

    更新日期:2017-08-08 00:00:00

  • Cloning and expression of afpA, a gene encoding an antifreeze protein from the arctic plant growth-promoting rhizobacterium Pseudomonas putida GR12-2.

    abstract::The Arctic plant growth-promoting rhizobacterium Pseudomonas putida GR12-2 secretes an antifreeze protein (AFP) that promotes survival at subzero temperatures. The AFP is unusual in that it also exhibits a low level of ice nucleation activity. A DNA fragment with an open reading frame encoding 473 amino acids was clon...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.17.5661-5671.2004

    authors: Muryoi N,Sato M,Kaneko S,Kawahara H,Obata H,Yaish MW,Griffith M,Glick BR

    更新日期:2004-09-01 00:00:00

  • Transcription of the Escherichia coli fumarate reductase genes (frdABCD) and their coordinate regulation by oxygen, nitrate, and fumarate.

    abstract::The fumarate reductase enzyme complex allows Escherichia coli to grow anaerobically with fumarate as a terminal electron acceptor for oxidative phosphorylation when the preferred compounds oxygen and nitrate are not available. We used the pKO promoter test vectors to identify a single promoter for the frdABCD genes wh...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.164.3.1100-1109.1985

    authors: Jones HM,Gunsalus RP

    更新日期:1985-12-01 00:00:00

  • Quorum sensing controls exopolysaccharide production in Sinorhizobium meliloti.

    abstract::Sinorhizobium meliloti is a soil bacterium capable of invading and establishing a symbiotic relationship with alfalfa plants. This invasion process requires the synthesis, by S. meliloti, of at least one of the two symbiotically important exopolysaccharides, succinoglycan and EPS II. We have previously shown that the ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.1.325-331.2003

    authors: Marketon MM,Glenn SA,Eberhard A,González JE

    更新日期:2003-01-01 00:00:00

  • Aerobic metabolism of Streptococcus agalactiae.

    abstract::Streptococcus agalactiae cultures possess an aerobic pathway for glucose oxidation that is strongly inhibited by cyanide. The products of glucose oxidation by aerobically grown cells of S. agalactiae 50 are lactic and acetic acids, acetylmethylcarbinol, and carbon dioxide. Glucose degradation products by aerobically g...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.94.1.184-191.1967

    authors: Mickelson MN

    更新日期:1967-07-01 00:00:00

  • Identification of a novel arsenite oxidase gene, arxA, in the haloalkaliphilic, arsenite-oxidizing bacterium Alkalilimnicola ehrlichii strain MLHE-1.

    abstract::Although arsenic is highly toxic to most organisms, certain prokaryotes are known to grow on and respire toxic metalloids of arsenic (i.e., arsenate and arsenite). Two enzymes are known to be required for this arsenic-based metabolism: (i) the arsenate respiratory reductase (ArrA) and (ii) arsenite oxidase (AoxB). Bot...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00244-10

    authors: Zargar K,Hoeft S,Oremland R,Saltikov CW

    更新日期:2010-07-01 00:00:00

  • Partial characterization of the factor responsible for tryptophanless death in Bacillus subtilis.

    abstract::The decline in colony-forming ability observed during tryptophan starvation of Bacillus subtilis auxotrophs is a concentration-dependent phenomenon. It does not manifest itself when the initial cell concentration is 10(6) cells/ml or lower. This property has been used to test the killing activity of different fraction...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.101.2.355-360.1970

    authors: Barlati S,Majerfeld I

    更新日期:1970-02-01 00:00:00

  • Transport of nonmetabolizable opines by Agrobacterium tumefaciens.

    abstract::We have examined the uptake of [14C]octopine and [14C]nopaline by Agrobacterium tumefaciens strains containing the C58 chromosomal background in medium suitable for the induction of vir genes. All strains tested could transport both of these opines, regardless of the presence or type of Ti plasmid (octopine or nopalin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.2.903-905.1991

    authors: Krishnan M,Burgner JW,Chilton WS,Gelvin SB

    更新日期:1991-01-01 00:00:00

  • Catechol 1,2-dioxygenase from Acinetobacter calcoaceticus: purification and properties.

    abstract::Procedures for the purification of catechol 1,2-dioxygenase from extracts of Acinetobacter calcoaceticus strain ADP-96 are described. The purified enzyme was homogeneous as judged by ultracentrifugation and acrylamide gel electrophoresis. The enzyme contained 2 g-atoms of iron per mol of protein. The enzyme had a broa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.127.1.536-544.1976

    authors: Patel RN,Hou CT,Felix A,Lillard MO

    更新日期:1976-07-01 00:00:00

  • Characterization of a mini-ColC1 plasmid.

    abstract::An in vitro constructed plasmid, pVH15, consisting of the entire genome of the plasmid ColE1, the tryptophan operon of Escherichia coli, and regions of the bacteriophage PHI80pt190, spontaneously gave rise in E. coli to a mini-ColE1 plasmid consisting of approximately one-half of the ColE1 genome and a small segment o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.126.1.447-453.1976

    authors: Hershfield V,Boyer HW,Chow L,Helinski DR

    更新日期:1976-04-01 00:00:00

  • Novel inner membrane retention signals in Pseudomonas aeruginosa lipoproteins.

    abstract::The ultimate membrane localization and function of most of the 185 predicted Pseudomonas aeruginosa PAO1 lipoproteins remain unknown. We constructed a fluorescent lipoprotein, CSFP(OmlA)-ChFP, by fusing the signal peptide and the first four amino acids of the P. aeruginosa outer membrane lipoprotein OmlA to the monome...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00603-08

    authors: Lewenza S,Mhlanga MM,Pugsley AP

    更新日期:2008-09-01 00:00:00

  • Membrane lipoteichoic acid is not a precursor to wall teichoic acid in pneumococci.

    abstract::The distribution of a pulse of teichoic acid-specific radiolabel between wall and membrane teichoic acids in pneumococci was constant over a subsequent chase period, suggesting that wall and membrane teichoic acids are biosynthesized simultaneously and independently. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.122.1.335-337.1975

    authors: Briles EB,Tomasz A

    更新日期:1975-04-01 00:00:00

  • Identification and characterization of a new porin gene of Klebsiella pneumoniae: its role in beta-lactam antibiotic resistance.

    abstract::Klebsiella pneumoniae porin genes were analyzed to detect mutations accounting for the porin deficiency observed in many beta-lactam-resistant strains. PCR and Southern blot analysis revealed the existence of a third porin gene in addition to the OmpK36 and OmpK35 porin genes previously described. This new porin gene ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.9.2726-2732.1999

    authors: Doménech-Sánchez A,Hernández-Allés S,Martínez-Martínez L,Benedí VJ,Albertí S

    更新日期:1999-05-01 00:00:00

  • Genetic locus (nmp-1) affecting the principal outer membrane protein of Neisseria gonorrhoeae.

    abstract::An increase in the apparent molecular weight of the principal outer membrane protein (POMP) of Neisseria gonorrhoeae is associated with introduction of the penB2 genetic marker, which results in low-level, relatively nonspecific antibiotic resistance. Limited proteolysis of the two forms of POMP showed that they had f...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.143.2.847-851.1980

    authors: Cannon JG,Klapper DG,Blackman EY,Sparling PF

    更新日期:1980-08-01 00:00:00

  • Regulation of lysine- and lysine-plus-threonine-inhibitable aspartokinases in Bacillus brevis.

    abstract::Further studies on the expression of the two aspartokinase activities in Bacillus bovis are presented. Aspartokinase I (previously shown to be inhibited and repressed by lysine) was found to be repressed by diaminopimelate in the wild-type strain. However, in a mutant unable to convert diaminopimelate to lysine, starv...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.142.2.424-432.1980

    authors: Hitchcock MJ,Hodgson B,Linforth JL

    更新日期:1980-05-01 00:00:00

  • Partial purification and properties of D-glucosamine 6'-phosphate N-acetyltransferase from zoospores of Blastocladiella emersonii.

    abstract::A d-glucosamine 6-phosphate N-acetyltransferase from Blastocladiella emersonii zoospores was partially purified to a specific activity of 2.41 IU per mg of protein. Its pH optimum was 8.05 and its K(m) values were 2.4 x 10(-4) M d-glucosamine 6-phosphate and 0.38 x 10(-4) M Na(3)S-acetyl coenzyme A. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.120.2.976-979.1974

    authors: Giddings TH,Cantino EC

    更新日期:1974-11-01 00:00:00

  • Cloning and sequencing of a cellobiose phosphotransferase system operon from Bacillus stearothermophilus XL-65-6 and functional expression in Escherichia coli.

    abstract::Cellulolytic strains of Bacillus stearothermophilus were isolated from nature and screened for the presence of activities associated with the degradation of plant cell walls. One isolate (strain XL-65-6) which exhibited strong activities with 4-methylumbelliferyl-beta-D-glucopyranoside (MUG) and 4-methylumbelliferyl-b...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.20.6441-6450.1993

    authors: Lai X,Ingram LO

    更新日期:1993-10-01 00:00:00

  • Sequence and distribution of IS1312: evidence for horizontal DNA transfer from Rhizobium meliloti to Agrobacterium tumefaciens.

    abstract::Two novel insertion sequences, IS1312 and IS1313, were found in pTiBo542, the Ti plasmid of Agrobacterium tumefaciens strains Bo542 and A281. Nucleotide sequencing and Southern hybridization revealed that IS1312 and IS1313 are homologous to Rhizobium meliloti ISRm1 and ISRm2, respectively. IS1312, ISRm1, and another A...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.9.2554-2559.1995

    authors: Deng W,Gordon MP,Nester EW

    更新日期:1995-05-01 00:00:00

  • USE OF S-METHYLCYSTINE AND CYSTATHIONINE BY METHIONINELESS NEUROSPORA MUTANTS.

    abstract::Wiebers, Joyce L. (Purdue University, West Lafayette, Ind.), and Harold R. Garner. Use of S-methylcysteine and cystathionine by methionineless Neurospora mutants. J. Bacteriol. 88:1798-1804. 1964.-Radioactive methionine was found in hydrolysates of various strains of Neurospora crassa when either S-methylcysteine (SMC...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.88.6.1798-1804.1964

    authors: WIEBERS JL,GARNER HR

    更新日期:1964-12-01 00:00:00

  • Two homologous genes coding for spore-specific proteins are expressed at different times during development of Myxococcus xanthus.

    abstract::The ops and tps genes of Myxococcus xanthus have ca. 90% DNA and amino acid sequence homology and are in the same orientation separated by a spacer region of only 1.4 kilobases. The products of the two genes were found to cross-react immunologically, and both were capable of Ca2+-dependent self-assembly on the surface...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.163.1.121-125.1985

    authors: Teintze M,Thomas R,Furuichi T,Inouye M,Inouye S

    更新日期:1985-07-01 00:00:00

  • A genetic mechanism for deletion of the ser2 gene cluster and formation of rough morphological variants of Mycobacterium avium.

    abstract::A major phenotypic trait of the Mycobacterium avium complex is the ability to produce rough and smooth colony variants. The chemical basis of this morphological variation is the loss of an antigenic surface structure, termed glycopeptidolipid (GPL), by rough variants. Using M. avium serovar 2 strain 2151 as a model sy...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.21.6177-6182.2000

    authors: Eckstein TM,Inamine JM,Lambert ML,Belisle JT

    更新日期:2000-11-01 00:00:00

  • Genetic analysis of the chitinase system of Serratia marcescens 2170.

    abstract::To carry out a genetic analysis of the degradation and utilization of chitin by Serratia marcescens 2170, various Tn5 insertion mutants with characteristic defects in chitinase production were isolated and partially characterized. Prior to the isolation of the mutants, proteins secreted into culture medium in the pres...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.22.7111-7117.1997

    authors: Watanabe T,Kimura K,Sumiya T,Nikaidou N,Suzuki K,Suzuki M,Taiyoji M,Ferrer S,Regue M

    更新日期:1997-11-01 00:00:00

  • Prediction of the rate of glucose utilization from cellular levels of glucose 6-phosphate and fructose 1,6-diphosphate in Escherichia coli.

    abstract::A comprehensive equation, upsilon = VM/[1 + (A0.5/Fru-P2)n] [ 1 + (Glc-6-P/I0.5)], has been proposed to represent the quantitative interrelationships between the rate of glucose utilization and the levels of glucose-6-phosphate and fructose-1,6-diphosphate in the intact Escherichia coli cell. This comprehensive equati...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.128.1.165-169.1976

    authors: Dietzler DN,Leckie MP,Crimmins DL,Ungar JM,Sternheim WL

    更新日期:1976-10-01 00:00:00

  • Enhanced acetohydroxy acid synthase III activity in an ilvH mutant of Escherichia coli K-12.

    abstract::The acetohydroxy acid synthase III isozyme, which catalyzes the first common step in the biosynthesis of isoleucine, leucine, and valine in Escherichia coli K-12, is composed of two subunits, the ilvI and ilvH gene products. A missense mutation in ilvH (ilvH612), which reduced the sensitivity of the enzyme to the end ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.11.5197-5199.1988

    authors: Ricca E,Limauro D,Lago CT,de Felice M

    更新日期:1988-11-01 00:00:00