Rapid identification of bacterial genes that are lethal when cloned on multicopy plasmids.

abstract：:The 2-(methylthio)ethanesulfonic acid (CH3-S-CoM) reductase catalyzes the final methane-yielding reaction in fastidiously anaerobic methanogenic archaebacteria. This step involves the reductive demethylation of CH3-S-CoM with reducing equivalents from N-7-(mercaptoheptanoyl)-L-threonine O3-phosphate (HS-HTP) to yield ...

journal_title：Journal of bacteriology

authors： Olson KD,Chmurkowska-Cichowlas L,McMahon CW,Wolfe RS

更新日期：1992-02-01 00:00:00

abstract：:Bacillus subtilis strains containing deletions in the genes coding for one or two of the major small, acid-soluble spore proteins (SASP; termed SASP-alpha and SASP-beta) were constructed. These mutants sporulated normally, but the spores lacked either SASP-alpha, SASP-beta, or both proteins. The level of minor SASP di...

journal_title：Journal of bacteriology

authors： Mason JM,Setlow P

更新日期：1986-07-01 00:00:00

abstract：:We isolated five new temperature-sensitive alleles of the essential cell division gene ftsZ in Escherichia coli, using P1-mediated, localized mutagenesis. The five resulting single amino acid changes (Gly109-->Ser109 for ftsZ6460, Ala129-->Thr129 for ftsZ972, Val157-->Met157 for ftsZ2066, Pro203-->Leu203 for ftsZ9124,...

journal_title：Journal of bacteriology

authors： Addinall SG,Small E,Whitaker D,Sturrock S,Donachie WD,Khattar MM

更新日期：2005-01-01 00:00:00

abstract：:The region of the colicin E1 polypeptide that interacts with immunity protein has been localized to a 168-residue COOH-terminal peptide. This is the length of a proteolytically generated peptide fragment of colicin E1 against which imm+ function can be demonstrated in osmotically shocked cells. The role of particular ...

journal_title：Journal of bacteriology

authors： Bishop LJ,Bjes ES,Davidson VL,Cramer WA

更新日期：1985-10-01 00:00:00

abstract：:The flagella of Methanococcus voltae were isolated by using three procedures. Initially, cells were sheared to release the filaments, which were purified by differential centrifugation and banding in KBr gradients. Flagella were also prepared by solubilization of cells with 1% (vol/vol) Triton X-100 and purified as de...

journal_title：Journal of bacteriology

authors： Kalmokoff ML,Jarrell KF,Koval SF

更新日期：1988-04-01 00:00:00

abstract：:We cloned and sequenced a fragment of the Bacillus stearothermophilus NUB36 chromosome that contains two open reading frames (ORFs) whose products were detected only in cells of cultures grown in complex medium at high temperature. The nucleotide sequence of the two ORFs exhibited significant identity to the sequence ...

journal_title：Journal of bacteriology

authors： Wu L,Welker NE

更新日期：1991-08-01 00:00:00

abstract：:Doxtader, K. G. (Cornell University, Ithaca, N.Y.), and M. Alexander. Role of 3-nitropropanoic acid in nitrate formation by Aspergillus flavus. J. Bacteriol. 91:1186-1191. 1966.-Aspergillus flavus formed nitrate, 3-nitropropanoic acid (3-NPA), kojic acid, and a substance tentatively identified as N-formyl-N-hydroxy-gl...

journal_title：Journal of bacteriology

authors： Doxtader KG,Alexander M

更新日期：1966-03-01 00:00:00

abstract：:Replication of the broad-host-range, IncPalpha plasmid RK2 requires two plasmid loci: trfA, the replication initiator gene, and oriV, the origin of replication. While these determinants are sufficient for replication in a wide variety of bacteria, they do not confer the stable maintenance of parental RK2 observed in i...

journal_title：Journal of bacteriology

authors： Rosche TM,Siddique A,Larsen MH,Figurski DH

更新日期：2000-11-01 00:00:00

abstract：:Acinetobacter baumannii has emerged as a significant nosocomial pathogen worldwide. The increasing trend of carbapenem and fluoroquinolone resistance in A. baumannii severely limits the usage of therapeutic antimicrobial agents. Here we report the genome sequence of a multidrug-resistant A. baumannii strain, TCDC-AB07...

journal_title：Journal of bacteriology

authors： Chen CC,Lin YC,Sheng WH,Chen YC,Chang SC,Hsia KC,Liao MH,Li SY

更新日期：2011-05-01 00:00:00

abstract：:In the filamentous cyanobacterium Anabaena variabilis, specialized cells called heterocysts occur in a regular pattern along the filament and are the sites of nitrogen fixation. We used two different types of DNA-excess RNA hybridization techniques to estimate the number of genes expressed in recently differentiated, ...

journal_title：Journal of bacteriology

authors： Lynn ME,Bantle JA,Ownby JD

更新日期：1986-09-01 00:00:00

abstract：:Previously in our laboratory, a PCR-based strategy was used to isolate potential sensor gene fragments from the Staphyloccus aureus genome. One DNA fragment was isolated that shared strong sequence similarity to genes encoding bacterial sensor proteins, indicating that it originated from within a potential staphylococ...

journal_title：Journal of bacteriology

authors： Brunskill EW,Bayles KW

更新日期：1996-02-01 00:00:00

abstract：:The cold shock response of Escherichia coli is elicited by downshift of temperature from 37 degrees C to 15 degrees C and is characterized by induction of several cold shock proteins, including CsdA, during the acclimation phase. CsdA, a DEAD-box protein, has been proposed to participate in a variety of processes, suc...

journal_title：Journal of bacteriology

authors： Awano N,Xu C,Ke H,Inoue K,Inouye M,Phadtare S

更新日期：2007-08-01 00:00:00

abstract：:A heat-stable, particulate, lipopolysaccharide-protein antigenic complex has been isolated from a virulent, encapsulated strain of Pasteurella multocida by extraction with cold, formalinized saline, and centrifugation at 105,000 x g. The original bacterial culture had been obtained from a bison that died of hemorrhagi...

journal_title：Journal of bacteriology

authors： Rebers PA,Heddleston KL,Rhoades KR

更新日期：1967-01-01 00:00:00

abstract：:We have characterized group A Streptococcus (GAS) genome-wide responses to hydrogen peroxide and assessed the role of the peroxide response regulator (PerR) in GAS under oxidative stress. Comparison of transcriptome changes elicited by peroxide in wild-type bacteria with those in a perR deletion mutant showed that 76 ...

journal_title：Journal of bacteriology

authors： Grifantini R,Toukoki C,Colaprico A,Gryllos I

更新日期：2011-12-01 00:00:00

abstract：:Acyl-homoserine lactone (acyl-HSL) quorum sensing is common to many Proteobacteria including a clinical isolate of Burkholderia cepacia. The B. cepacia isolate produces low levels of octanoyl-HSL. We have examined an environmental isolate of Burkholderia vietnamiensis. This isolate produced several acyl-HSLs. The most...

journal_title：Journal of bacteriology

authors： Conway BA,Greenberg EP

更新日期：2002-02-01 00:00:00

abstract：:A primer extension inhibition (toeprint) assay was developed using ribosomes and ribosomal subunits from Streptomyces lividans. This assay allowed the study of ribosome binding to streptomycete leaderless and leadered mRNA. Purified 30S subunits were unable to form a ternary complex on aph leaderless mRNA, whereas 70S...

journal_title：Journal of bacteriology

authors： Day JM,Janssen GR

更新日期：2004-10-01 00:00:00

abstract：:Bacterial spores have long been recognized as the sturdiest known life forms on earth, revealing extraordinary resistance to a broad range of environmental assaults. A family of highly conserved spore-specific DNA-binding proteins, termed alpha/beta-type small, acid-soluble spore proteins (SASP), plays a major role in...

journal_title：Journal of bacteriology

authors： Frenkiel-Krispin D,Sack R,Englander J,Shimoni E,Eisenstein M,Bullitt E,Horowitz-Scherer R,Hayes CS,Setlow P,Minsky A,Wolf SG

更新日期：2004-06-01 00:00:00

abstract：:Desulfurococcus kamchatkensis is an anaerobic organotrophic hyperthermophilic crenarchaeon isolated from a terrestrial hot spring. Its genome consists of a single circular chromosome of 1,365,223 bp with no extrachromosomal elements. A total of 1,474 protein-encoding genes were annotated, among which 205 are exclusive...

journal_title：Journal of bacteriology

authors： Ravin NV,Mardanov AV,Beletsky AV,Kublanov IV,Kolganova TV,Lebedinsky AV,Chernyh NA,Bonch-Osmolovskaya EA,Skryabin KG

更新日期：2009-04-01 00:00:00

abstract：:The Escherichia coli secA gene, whose expression is responsive to the protein secretion status of the cell, is the second gene in an operon. We found that both the basal and induced levels of SecA biosynthesis are dependent on prior translation of the upstream gene, gene X, and identified two large gene X-secA transcr...

journal_title：Journal of bacteriology

authors： Schmidt MG,Oliver DB

更新日期：1989-02-01 00:00:00

abstract：:pBL2 was identified genetically but not physically in Streptomyces lividans after its mating with S. bambergiensis. During conjugation, pBL2 was transferred at high frequency to S. lividans and S. coelicolor. pBL2.1 DNA isolated from S. coelicolor exconjugants as a circular plasmid was shown to derive from the genome ...

journal_title：Journal of bacteriology

authors： Zotchev SB,Schrempf H,Hutchinson CR

更新日期：1995-08-01 00:00:00

abstract：:The ability to restart broken DNA replication forks is essential across all domains of life. In Escherichia coli, the priA, priB, priC, and dnaT genes encode the replication restart proteins (RRPs) to accomplish this task. PriA plays a critical role in replication restart such that its absence reveals a dramatic pheno...

journal_title：Journal of bacteriology

authors： Leroux M,Jani N,Sandler SJ

更新日期：2017-08-08 00:00:00

abstract：:The gene (entD) encoding staphylococcal enterotoxin D (SED) has been located on a 27.6-kilobase penicillinase plasmid designated pIB485. This plasmid was present in all SED-producing strains tested. The entD gene was cloned on a 2.0-kilobase DNA fragment and was expressed in Escherichia coli. Sequence analysis of this...

journal_title：Journal of bacteriology

authors： Bayles KW,Iandolo JJ

更新日期：1989-09-01 00:00:00

abstract：:In studies of Pseudomonas putida IH-2000, a toluene-tolerant microorganism, membrane vesicles (MVs) were found to be released from the outer membrane when toluene was added to the culture. These MVs were found to be composed of phospholipids, lipopolysaccharides (LPS), and very low amounts of outer membrane proteins. ...

journal_title：Journal of bacteriology

authors： Kobayashi H,Uematsu K,Hirayama H,Horikoshi K

更新日期：2000-11-01 00:00:00

abstract：:A novel regulatory gene, trh, which is involved in hrp gene expression, is identified in the plant pathogen Xanthomonas oryzae pv. oryzae. In the trh mutant, expression of HrpG, which is a key regulator for hrp gene expression, is reduced both under the in vitro hrp-inducing condition and in planta. ...

journal_title：Journal of bacteriology

authors： Tsuge S,Nakayama T,Terashima S,Ochiai H,Furutani A,Oku T,Tsuno K,Kubo Y,Kaku H

更新日期：2006-06-01 00:00:00

abstract：:Branching enzyme (BE) catalyzes formation of the branch points in glycogen and amylopectin by cleavage of the alpha-1,4 linkage and its subsequent transfer to the alpha-1,6 position. We have identified a novel BE encoded by an uncharacterized open reading frame (TK1436) of the hyperthermophilic archaeon Thermococcus k...

journal_title：Journal of bacteriology

authors： Murakami T,Kanai T,Takata H,Kuriki T,Imanaka T

更新日期：2006-08-01 00:00:00

abstract：:Wzc(CPS) is a tyrosine autokinase essential for the assembly of a high-molecular-weight (HMW) group 1 capsular polysaccharide (CPS) in Escherichia coli. Homologues of Wzc participate in the formation of CPS and exopolysaccharides in a variety of gram-positive and gram-negative bacteria. Phosphorylation of tyrosine res...

journal_title：Journal of bacteriology

authors： Paiment A,Hocking J,Whitfield C

更新日期：2002-12-01 00:00:00

abstract：:In wild-type Escherichia coli, fumarate reductase encoded by the frd operon is inducible by its substrate in the absence of molecular oxygen and nitrate. Synthesis of this enzyme under permissive conditions requires the fnr+ gene product, which is believed to be a pleiotropic regulatory protein that activates transcri...

journal_title：Journal of bacteriology

authors： Iuchi S,Kuritzkes DR,Lin EC

更新日期：1985-03-01 00:00:00

abstract：:The synthesis of pyruvate carboxylase (PC) was studied by using quantitative immunoblot analysis with an antibody raised against PC purified from Rhodobacter capsulatus and was found to vary 20-fold depending on the growth conditions. The PC content was high in cells grown on pyruvate or on carbon substrates metaboliz...

journal_title：Journal of bacteriology

authors： Yakunin AF,Hallenbeck PC

更新日期：1997-03-01 00:00:00

abstract：:Among strains of Haemophilus influenzae, the ability to catabolize tryptophan (as detected by indole production) varies and is correlated with pathogenicity. Tryptophan catabolism is widespread (70 to 75%) among harmless respiratory isolates but is nearly universal (94 to 100%) among strains causing serious disease, i...

journal_title：Journal of bacteriology

authors： Martin K,Morlin G,Smith A,Nordyke A,Eisenstark A,Golomb M

更新日期：1998-01-01 00:00:00

abstract：:The R1162-encoded protein RepIB is essential for replication of the plasmid and binds specifically to iterons within the replicative origin. The protein causes the localized melting of DNA (determined by sensitivity to P1 nuclease) at a site within the AT-rich region of the origin, about 60 bp from the iteron binding ...

journal_title：Journal of bacteriology

authors： Kim YJ,Meyer RJ

更新日期：1991-09-01 00:00:00