Abstract:
:Acyl-homoserine lactone (acyl-HSL) quorum sensing is common to many Proteobacteria including a clinical isolate of Burkholderia cepacia. The B. cepacia isolate produces low levels of octanoyl-HSL. We have examined an environmental isolate of Burkholderia vietnamiensis. This isolate produced several acyl-HSLs. The most abundant species was decanoyl-HSL. Decanoyl-HSL in B. vietnamiensis cultures reached concentrations in excess of 20 microM. We isolated a B. vietnamiensis DNA fragment containing a gene for the synthesis of decanoyl-HSL (bviI) and an open reading frame that codes for a putative signal receptor (bviR). A B. vietnamiensis bviI mutant did not produce detectable levels of decanoyl-HSL.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Conway BA,Greenberg EPdoi
10.1128/jb.184.4.1187-1191.2002keywords:
subject
Has Abstractpub_date
2002-02-01 00:00:00pages
1187-91issue
4eissn
0021-9193issn
1098-5530journal_volume
184pub_type
杂志文章abstract::In Salmonella, ilv-linked rfe genes participate in the biosynthesis of the enterobacterial common antigen (CA) as well as of certain types of O antigen (serogroups C1 and L). rff genes, probably in the same cluster with rfe, are required for CA synthesis (P.H. Mäkelä et al., in preparation). Several Escherichia coli s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.127.2.755-762.1976
更新日期:1976-08-01 00:00:00
abstract::Our current understanding of segregation of prokaryotic plasmids has been derived mainly from the study of the gram-negative bacterial plasmids. We previously reported a replicon of the cryptic plasmid from a gram-positive bacterium, Leifsonia xyli subsp. cynodontis. The replicon contains a putative plasmid partition ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01232-06
更新日期:2006-12-01 00:00:00
abstract::The polymerase chain reaction (PCR) has been used to amplify DNA fragments by using eucaryotic genomic DNA as a template. We show that bacterial genomic DNA can be used as a template for PCR amplification. We demonstrate that DNA fragments at least as large as 4,400 base pairs can be amplified with fidelity and that t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.3.1602-1608.1989
更新日期:1989-03-01 00:00:00
abstract::Shewanella putrefaciens strain 200 respires a wide range of compounds as terminal electron acceptor. The respiratory versatility of Shewanella is attributed in part to a set of c-type cytochromes with widely varying midpoint redox potentials (E'(0)). A point mutant of S. putrefaciens, originally designated Urr14 and h...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01249-06
更新日期:2007-02-01 00:00:00
abstract::The folded chromosome of Escherichia coli has been investigated under various lysis and physiological conditions. A new gradient system was devised that allows excellent separation between unlysed cells and envelope-associated and envelope-free chromosomes. Isotope incorporation experiments showed that the fraction of...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.127.2.904-916.1976
更新日期:1976-08-01 00:00:00
abstract::In Escherichia coli cultures maximally inhibited with chloramphenicol, formation of polypeptides still continued at a slow, constant rate for at least 90 min. The rate of leucine incorporation was reduced to 0.5%, but methionine was only reduced to 2%, suggesting that chains are normally initiated with methionine but ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.118.2.582-589.1974
更新日期:1974-05-01 00:00:00
abstract::The TcbR transcriptional activator protein, which is encoded by the tcbR gene of Pseudomonas sp. strain P51 (J. R. van der Meer, A. C. J. Frijters, J. H. J. Leveau, R. I. L. Eggen, A. J. B. Zehnder, and W. M. de Vos, J. Bacteriol. 173:3700-3708, 1991), was purified from overproducing Escherichia coli cells by using a ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.7.1850-1856.1994
更新日期:1994-04-01 00:00:00
abstract::Pulse labeling of deoxyribonucleic acid (DNA) and radioautography have been used to study the effect of growth rate on nuclear replication in Aspergillus nidulans. When conidia were germinated in media supporting a fast growth rate, the radioactive pulse labeled either all of the nuclei in a cell or none of them. At s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.6.2275-2281.1968
更新日期:1968-06-01 00:00:00
abstract::The temperate phage TPW22, induced from Lactococcus lactis subsp. cremoris W22, and the evolutionarily interesting integrase of this phage were characterized. Phage TPW22 was propagated lytically on L. lactis subsp. cremoris 3107, which could also be lysogenized by site-specific integration. The attachment site (attP)...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.22.7034-7042.1999
更新日期:1999-11-01 00:00:00
abstract::The growth of tubercle bacilli in serum samples of untreated animals depends upon the availability of ionic iron which serves as a growth factor in supporting bacillary multiplication. The amount of available iron in serum is determined by the ratio between iron-saturated and iron-free transferrin; a low value for the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.1.64-70.1969
更新日期:1969-10-01 00:00:00
abstract::We have carefully characterized and reexamined the motility and phototactic responses of Synechocystis sp. adenylyl cyclase (Cya1) and catabolite activator protein (SYCRP1) mutants to different light regimens, glucose, 3-(3,4-dichlorophenyl)-1,1-dimethylurea, and cyclic AMP. We find that contrary to earlier reports, c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00573-06
更新日期:2006-10-01 00:00:00
abstract::Several resistant mutants of a transformable group H streptococcus, strain Challis, were isolated from media containing high concentrations of streptomycin. Mutants SR5a and SR5 exhibited high and low transformability, respectively, when exposed to deoxyribonucleic acid (DNA) from a novobiocin-resistant Challis strain...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.1.132-138.1968
更新日期:1968-01-01 00:00:00
abstract::Thrust for propulsion of flagellated bacteria is generated by rotation of a propeller, the flagellum. The power to drive the polar flagellar rotary motor of Vibrio parahaemolyticus is derived from the transmembrane potential of sodium ions. Force is generated by the motor on coupling of the movement of ions across the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.19.5988-5998.1994
更新日期:1994-10-01 00:00:00
abstract::Gundersen, Wenche B. (Oslo University, Oslo, Norway). New type of streptomycin resistance resulting from action of the episomelike mutator factor in Escherichia coli. J. Bacteriol. 86:510-516. 1963.-Analyses have been performed to elucidate the genetic nature of the streptomycin resistance that results from the action...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.86.3.510-516.1963
更新日期:1963-09-01 00:00:00
abstract::Transposon Tn917 mutagenesis of Bacillus subtilis BD99 followed by selection for protonophore resistance led to the isolation of strain MS119, which contained a single Tn917 insertion in an open reading frame whose deduced amino acid sequence was 56.6% identical to that of the Escherichia coli rho gene product. The in...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.3.647-654.1993
更新日期:1993-02-01 00:00:00
abstract::The gene responsible for the uncommon glutamate dehydrogenase (GDH) activity of Lactococcus lactis was identified and characterized. It encodes a GDH of family I that is mainly active in glutamate biosynthesis, is carried by a large plasmid, and is included, with functional cadmium resistance genes, in a remnant Tn3-l...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.14.5019-5022.2005
更新日期:2005-07-01 00:00:00
abstract::After the Bacillus subtilis nucleoid was dissected with restriction endonucleases, a specific DNA sequence from the purA region was isolated in a particulate form that probably originated from the cell membrane. Precise definition of the binding region within this sequence was achieved by a novel procedure based on a ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.166.1.38-43.1986
更新日期:1986-04-01 00:00:00
abstract:UNLABELLED:Corynebacterium glutamicum metabolizes sialic acid (Neu5Ac) to fructose-6-phosphate (fructose-6P) via the consecutive activity of the sialic acid importer SiaEFGI, N-acetylneuraminic acid lyase (NanA), N-acetylmannosamine kinase (NanK), N-acetylmannosamine-6P epimerase (NanE), N-acetylglucosamine-6P deacetyl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00820-15
更新日期:2016-07-28 00:00:00
abstract::RpoS is a conserved alternative sigma factor that regulates the expression of many stress response genes in Escherichia coli. The RpoS regulon is large but has not yet been completely characterized. In this study, we report the identification of over 100 RpoS-dependent fusions in a genetic screen based on the differen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.24.8499-8507.2004
更新日期:2004-12-01 00:00:00
abstract::Erwinia rhapontici is able to convert sucrose into isomaltulose (palatinose, 6-O-alpha-D-glucopyranosyl-D-fructose) and trehalulose (1-O-alpha-D-glucopyranosyl-D-fructose) by the activity of a sucrose isomerase. These sucrose isomers cannot be metabolized by plant cells and most other organisms and therefore are possi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.8.2425-2430.2001
更新日期:2001-04-01 00:00:00
abstract::Proteomics and targeted gene disruption were used to investigate the catabolism of benzene, styrene, biphenyl, and ethylbenzene in Rhodococcus jostii RHA1, a well-studied soil bacterium whose potent polychlorinated biphenyl (PCB)-transforming properties are partly due to the presence of the related Bph and Etb pathway...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01122-07
更新日期:2008-01-01 00:00:00
abstract::RsbT is an essential component of the pathway that activates the Bacillus subtilis sigma(B) transcription factor in response to physical stress. rsbT is located within an operon that includes the genes for its principal negative regulator (RsbS) and the stress pathway component that it activates (RsbU), as immediate u...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.24.8520-8525.2005
更新日期:2005-12-01 00:00:00
abstract::The thiol-containing tripeptide glutathione is an important cellular constituent of many eukaryotic and prokaryotic cells. In addition to its disulfide reductase activity, glutathione is known to protect cells from many forms of physiological stress. This report represents the first investigation into the role of glut...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01393-12
更新日期:2012-11-01 00:00:00
abstract::The mecA-27r gene, which encodes PBP2a-27r, was modified by site-specific mutagenesis, resulting in replacement of the N-terminal membrane anchor with a short chelating peptide (CP-PBP2a-27r). CP-PBP2a-27r retained the same binding affinity for beta-lactam antibiotics as the wild-type enzyme. Approximately 95% pure CP...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.5.1539-1541.1994
更新日期:1994-03-01 00:00:00
abstract::Triggering of germination in Bacillus megaterium QM B1551 spores with D-glucose was studied. First, the interaction of glucose with spores for less than 1 min resulted in triggering almost 90% of the spores after the glucose was removed by dilution. Therefore only a brief time is needed for glucose to trigger germinat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.138.2.442-445.1979
更新日期:1979-05-01 00:00:00
abstract::When Methanobacterium thermoautotrophicum cells were incubated in 50 mM potassium phosphate buffer (pH 7.0) containing 1 M sucrose and autolysate from Methanobacterium wolfei, they were transformed into protoplasts. The protoplasts, which possessed no cell wall, lysed in buffer without sucrose. Unlike whole cells, the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.168.2.892-900.1986
更新日期:1986-11-01 00:00:00
abstract::Most bacteriophages (phages) release their progeny through the action of holins that form lesions in the cytoplasmic membrane and lysins that degrade the bacterial peptidoglycan. Although the function of each protein is well established in phages infecting Streptococcus pneumoniae, the role--if any--of the powerful ba...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00477-09
更新日期:2009-09-01 00:00:00
abstract::Ralston, D. J. (University of California, Berkeley) and M. D. Perry. Inhibitory action of phage K on staphylococcal dehydrogenases. II. Its possible relationship to sensitization and cell lysis. J. Bacteriol. 86:740-748. 1963.-By measuring the reduction of the dye triphenyl tetrazolium chloride to the insoluble red fo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.86.4.740-748.1963
更新日期:1963-10-01 00:00:00
abstract::Studies on the composition of total fatty acids of Alcaligenes faecalis harvested at different growth phases have been carried out. Ability of the organism to desaturate palmitic and stearic acid has also been tested. The organism contained palmitic (16:0), stearic (18:0), palmitoleic (16:1), cis-vaccenic (18:1), cycl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.114.2.618-624.1973
更新日期:1973-05-01 00:00:00
abstract::The two closely linked fdhD and fdhE genes of Escherichia coli are required for the formation of active membrane-bound phenazine methosulfate-linked formate dehydrogenase (FDH-PMS). Both genes were isolated from a cosmid library. Restriction endonuclease analysis associated with Mu dII1734 insertion mutagenesis indica...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.10.6112-6121.1990
更新日期:1990-10-01 00:00:00