Abstract:
:In Escherichia coli cultures maximally inhibited with chloramphenicol, formation of polypeptides still continued at a slow, constant rate for at least 90 min. The rate of leucine incorporation was reduced to 0.5%, but methionine was only reduced to 2%, suggesting that chains are normally initiated with methionine but are prematurely released at a short chain length. Consistent with this possibility was the distribution of the products on Sephadex columns: a range of peptides longer than 4 and shorter than 60 to 70 residues was seen. Less than 10% of the peptides broke down during a chase with cold amino acids, and during continuous labeling they accumulated progressively. On the average, one peptide was formed per ribosome every 5 min. Peptide synthesis in the presence of chloramphenicol was still dependent on ribosome translocation; it stopped in a mutant with an inactivated temperature-sensitive elongation factor G. But even in the absence of translocation, new messenger ribonucleic acid (mRNA) chains were found joined to one or a few ribosomes. The chains had a size distribution comparable to that of mRNA from polyribosomes of growing cells. They were stabilized for an average time of about 5 min, but were more rapidly degraded after puromycin was added to the cells. This suggests that stabilization may be related to the average time spent by a ribosome on an mRNA chain, with or without polypeptide formation.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Cremer K,Silengo L,Schlessinger Ddoi
10.1128/JB.118.2.582-589.1974subject
Has Abstractpub_date
1974-05-01 00:00:00pages
582-9issue
2eissn
0021-9193issn
1098-5530journal_volume
118pub_type
杂志文章abstract::The mocC gene encoded by the octopine/mannityl opine-type Ti plasmid pTi15955 is related at the nucleotide sequence level to mas1' encoded by the T region of this plasmid. While Mas1 is required for the synthesis of mannopine (MOP) by crown gall tumor cells, MocC is essential for the utilization of MOP by Agrobacteriu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.11.3285-3292.1996
更新日期:1996-06-01 00:00:00
abstract::Previously in our laboratory, a PCR-based strategy was used to isolate potential sensor gene fragments from the Staphyloccus aureus genome. One DNA fragment was isolated that shared strong sequence similarity to genes encoding bacterial sensor proteins, indicating that it originated from within a potential staphylococ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.3.611-618.1996
更新日期:1996-02-01 00:00:00
abstract::Glucose uptake by Bacteroides succinogenes S85 was measured under conditions that maintained anaerobiosis and osmotic stability. Uptake was inhibited by compounds which interfere with electron transport systems, maintenance of proton or metal ion gradients, or ATP synthesis. The most potent inhibitors were proton and ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.2.500-506.1987
更新日期:1987-02-01 00:00:00
abstract::The weak organic acid sorbic acid is a commonly used food preservative, as it inhibits the growth of bacteria, yeasts, and molds. We have used genome-wide transcriptional profiling of Bacillus subtilis cells during mild sorbic acid stress to reveal the growth-inhibitory activity of this preservative and to identify po...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01516-07
更新日期:2008-03-01 00:00:00
abstract::Serpulina hyodysenteriae, the etiologic agent of swine dysentery, contains complex periplasmic flagella which are composed of multiple class A and class B polypeptides. To examine the role these proteins play in flagellar synthesis, structure, and function and to develop strains which may provide insight into the impo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.20.5959-5970.1995
更新日期:1995-10-01 00:00:00
abstract::Desulfosporosinus species are sulfate-reducing bacteria belonging to the Firmicutes. Their genomes will give insights into the genetic repertoire and evolution of sulfate reducers typically thriving in terrestrial environments and able to degrade toluene (Desulfosporosinus youngiae), to reduce Fe(III) (Desulfosporosin...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01392-12
更新日期:2012-11-01 00:00:00
abstract::A gene suppressing the defects of Escherichia coli rdgB (recA-dependent growth) and recA(Ts) rdgB mutants was cloned. The cloned gene suppresses the hyper-Rec phenotype and the enhanced level of SOS functions in rdgB mutants at the nonpermissive temperature. We identified the cloned gene as purA. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.3.1360-1362.1991
更新日期:1991-02-01 00:00:00
abstract::Mutants of Thiosphaera pantotropha defective in chemolithoautotrophic growth were obtained by transpositional mutagenesis with Tn5 coding for kanamycin resistance. The suicide vehicle for introducing Tn5 to T. pantotropha was pSUP5011 harbored by Escherichia coli. Kanamycin-resistant isolates were screened for the ina...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.166.2.446-452.1986
更新日期:1986-05-01 00:00:00
abstract::Cystic fibrosis transmembrane conductance regulator (CFTR) inhibitory factor (Cif) is a virulence factor secreted by Pseudomonas aeruginosa that reduces the quantity of CFTR in the apical membrane of human airway epithelial cells. Initial sequence analysis suggested that Cif is an epoxide hydrolase (EH), but its seque...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01348-09
更新日期:2010-04-01 00:00:00
abstract::The shufflon, a multiple DNA inversion system in the plasmid R64, consists of four DNA segments flanked and separated by seven 19-bp repeat sequences. Site-specific recombinations mediated by the rci product occur between each inverted repeat sequence, resulting in inversions of the four segments independently or in g...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.6.1867-1871.1997
更新日期:1997-03-01 00:00:00
abstract:UNLABELLED:The airways of patients with cystic fibrosis are colonized with diverse bacterial communities that change dynamically during pediatric years and early adulthood. Staphylococcus aureus is the most prevalent pathogen during early childhood, but during late teens and early adulthood, a shift in microbial compos...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00059-15
更新日期:2015-07-01 00:00:00
abstract::Fundamental questions in bacterial gene regulation concern how multiple regulatory proteins interact with the transcription apparatus at a single promoter and what are the roles of protein contacts with RNA polymerase and changes in DNA conformation. Transcription of the Escherichia coli uhpT gene, encoding the induci...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.23.7266-7273.1999
更新日期:1999-12-01 00:00:00
abstract::During sporulation, the Bacillus subtilis transcription factor sigmaK is activated by regulated proteolytic processing. I have used a system that facilitates the analysis of the contributions of a modified form of the processing enzyme, SpoIVFB-GFP, and the regulatory proteins BofA and SpoIVFA to the conversion of pro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.17.5384-5388.1999
更新日期:1999-09-01 00:00:00
abstract::ULTRACENTRIFUGATION IN SUCROSE DENSITY GRADIENTS WAS EMPLOYED TO ESTIMATE THE MOLECULAR WEIGHTS AND TO DETERMINE POSSIBLE PHYSICAL AGGREGATION OF THE FIVE ENZYMES CATALYZING STEPS TWO TO SIX IN THE PRECHORISMIC ACID PORTION OF THE POLYAROMATIC SYNTHETIC PATHWAY IN SIX SPECIES OF BACTERIA: Escherichia coli, Salmonella ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.99.1.222-230.1969
更新日期:1969-07-01 00:00:00
abstract::Fixation of NaH(14)CO(3) by a heavy cell suspension of Streptococcus faecalis var. liquefaciens was studied. Several nutrients, pyridoxal, riboflavine, adenine, uracil, and O(2) stimulated (14)CO(2) incorporation into cells only under conditions that were adequate for synthesis of cell macromolecules. Biotin increased...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.104.1.27-33.1970
更新日期:1970-10-01 00:00:00
abstract::Transcriptional factor CarD is the only reported prokaryotic analog of eukaryotic high-mobility-group A (HMGA) proteins, in that it has contiguous acidic and AT hook DNA-binding segments and multifunctional roles in Myxococcus xanthus carotenogenesis and fruiting body formation. HMGA proteins are small, randomly struc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.12.3527-3537.2003
更新日期:2003-06-01 00:00:00
abstract::The two-component regulatory system, composed of virA and virG, is indispensable for transcription of virulence genes within Agrobacterium tumefaciens. However, virA and virG are insufficient to activate transcription from virulence gene promoters within Escherichia coli cells, indicating a requirement for additional ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.15.4533-4539.1999
更新日期:1999-08-01 00:00:00
abstract::Cells of Candida albicans WO-1 switch frequently and reversibly between two colony-forming phenotypes, white and opaque. In the white form, budding cells appear similar to those of most other strains of C. albicans, but in the opaque form, budding cells are larger, are bean shaped, and possess pimples on the wall. The...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.1.224-235.1990
更新日期:1990-01-01 00:00:00
abstract::Outer membranes, free of cytoplasmic or thylakoid membranes and peptidoglycan components, were obtained from Synechocystis sp. strain PCC6714. Electron microscope studies revealed double-track outer membrane vesicles with a smooth-appearing exoplasmic surface, an exoplasmic fracture face covered by closely packed part...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.1.384-389.1985
更新日期:1985-10-01 00:00:00
abstract::We have cloned fragments of DNA (up to 13 kb), from Pseudomonas putida AJ1, that code for two stereospecific haloalkanoate dehalogenases. These enzymes are highly specific for D and L substrates. The two genes, designated hadD and hadL, have been isolated and independently expressed in Escherichia coli and P. putida h...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.8.2612-2619.1992
更新日期:1992-04-01 00:00:00
abstract::Impurities believed to be polysaccharides have been found in mycobacterial deoxyribonucleic acid (DNA) preparations. Agar-gel diffusion of the DNA preparations against concanavalin A indicated the presence of three polysaccharides and was used to follow the purification procedures. The polysaccharides appeared to be t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.112.3.1033-1039.1972
更新日期:1972-12-01 00:00:00
abstract::The Ssa subfamily of Hsp70 molecular chaperones in the budding yeast Saccharomyces cerevisiae has four members, encoded by SSA1, SSA2, SSA3, and SSA4. Deletion of the two constitutively expressed genes, SSA1 and SSA2, results in cells which are slow growing and temperature sensitive. In this study, we demonstrate that...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.24.6484-6492.1998
更新日期:1998-12-01 00:00:00
abstract::Bacteriophage N4 middle genes are transcribed by a phage-coded, heterodimeric, rifampin-resistant RNA polymerase, N4 RNA polymerase II (N4 RNAPII). Sequencing and transcriptional analysis revealed that the genes encoding the two subunits comprising N4 RNAPII are translated from a common transcript initiating at the N4...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.18.4952-4961.2002
更新日期:2002-09-01 00:00:00
abstract::Mutation of the spoVB gene in Bacillus subtilis causes the production of spores containing a defective cortex and unable to acquire heat resistance. The spoVB locus is highly linked to another spo locus, spoIIIF, characterized by a single mutation (I. L. Lamont and J. Mandelstam, J. Gen. Microbiol. 130:1253-1261, 1984...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.24.7942-7949.1991
更新日期:1991-12-01 00:00:00
abstract::Specific changes in the chemical and microbial composition of Thermoanaerobium brockii fermentations were compared and related to alterations of process rates, end product yields, and growth parameters. Fermentation of starch as compared with glucose was associated with significant decreases in growth rate and intrace...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.146.1.192-199.1981
更新日期:1981-04-01 00:00:00
abstract::Exposure of Escherichia coli strains deficient in molybdopterin biosynthesis (moa) to the purine base N-6-hydroxylaminopurine (HAP) is mutagenic and toxic. We show that moa mutants exposed to HAP also exhibit elevated mutagenesis, a hyperrecombination phenotype, and increased SOS induction. The E. coli rdgB gene encod...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.10.3101-3110.2003
更新日期:2003-05-01 00:00:00
abstract::Nakata, H. M. (Washington State University, Pullman). Effect of pH on intermediates produced during growth and sporulation of Bacillus cereus. J. Bacteriol. 86:577-581. 1963.-Cultures of Bacillus cereus strain T, grown in an unbuffered glucose-yeast extract-mineral salts medium and in the same medium buffered at pH 6....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.86.3.577-581.1963
更新日期:1963-09-01 00:00:00
abstract::Zymomonas mobilis ZM401 is a flocculating strain which can be self-immobilized within fermentors for a high-cell-density culture to improve ethanol productivity, as well as high-gravity fermentation to increase ethanol titer, due to its improved ethanol tolerance associated with the morphological change. Here, we repo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01947-12
更新日期:2012-12-01 00:00:00
abstract::A mutant of Pseudomonas aeruginosa severely deficient in outer membrane protein F levels was isolated by screening heavily mutagenized strains for membrane protein alterations on sodium dodecyl sulphate-polyacrylamide gel electrophoresis. To provide a basis for phenotypic comparison, three independent spontaneous reve...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.1.281-285.1983
更新日期:1983-01-01 00:00:00
abstract::Of 16 restriction endonucleases known to hydrolyze rare 6- or 8-base recognition sequences that were tested, only SpeI, NotI, AscI, and SfiI generated fragments of chromosomal DNA from Yersinia pestis, the causative agent of bubonic plague, of sufficient length to permit physical analysis by pulsed-field gel electroph...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.7.2078-2086.1992
更新日期:1992-04-01 00:00:00