Purification of mycobacterial deoxyribonucleic acid.

abstract：:Mycobacterial genomes are endowed with many eukaryote-like nucleotide cyclase genes encoding proteins that can synthesize 3',5'-cyclic AMP (cAMP). However, the roles of cAMP and the need for such redundancy in terms of adenylyl cyclase genes remain unknown. We measured cAMP levels in Mycobacterium smegmatis during gro...

journal_title：Journal of bacteriology

authors： Dass BK,Sharma R,Shenoy AR,Mattoo R,Visweswariah SS

更新日期：2008-06-01 00:00:00

abstract：:We present the complete genome sequence of Listeria welshimeri, a nonpathogenic member of the genus Listeria. Listeria welshimeri harbors a circular chromosome of 2,814,130 bp with 2,780 open reading frames. Comparative genomic analysis of chromosomal regions between L. welshimeri, Listeria innocua, and Listeria monoc...

journal_title：Journal of bacteriology

authors： Hain T,Steinweg C,Kuenne CT,Billion A,Ghai R,Chatterjee SS,Domann E,Kärst U,Goesmann A,Bekel T,Bartels D,Kaiser O,Meyer F,Pühler A,Weisshaar B,Wehland J,Liang C,Dandekar T,Lampidis R,Kreft J,Goebel W,Chakraborty

更新日期：2006-11-01 00:00:00

abstract：:Transposition is a DNA reorganization reaction potentially deleterious for the host. The frequency of transposition is limited by the amount of transposase. Therefore, strict regulation of a transposase is required to keep control over the destructive multiplication of the mobile element. We have shown previously that...

journal_title：Journal of bacteriology

authors： Hõrak R,Kivisaar M

更新日期：1999-10-01 00:00:00

abstract：:Type IV secretion systems (T4SS) mediate the transfer of DNA and protein substrates to target cells. TrwK, encoded by the conjugative plasmid R388, is a member of the VirB4 family, comprising the largest and most conserved proteins of T4SS. VirB4 was suggested to be an ATPase involved in energizing pilus assembly and ...

journal_title：Journal of bacteriology

authors： Arechaga I,Peña A,Zunzunegui S,del Carmen Fernández-Alonso M,Rivas G,de la Cruz F

更新日期：2008-08-01 00:00:00

abstract：:The Arc (anoxic redox control) two-component signal transduction system of Escherichia coli, which comprises the tripartite ArcB sensor kinase and the ArcA response regulator, modulates the expression of numerous operons in response to redox conditions of growth. We demonstrate that the arcA and arcB genes of Haemophi...

journal_title：Journal of bacteriology

authors： Georgellis D,Kwon O,Lin EC,Wong SM,Akerley BJ

更新日期：2001-12-01 00:00:00

abstract：:An enzyme which liberates Pi from myo-inositol hexaphosphate (phytic acid) was shown to be present in culture filtrates of Bacillus subtilis. It was purified until it was homogeneous by ultracentrifugation, but it still showed two isozymes on polyacrylamide gel electrophoresis. The enzyme differed from other previousl...

journal_title：Journal of bacteriology

authors： Powar VK,Jagannathan V

更新日期：1982-09-01 00:00:00

abstract：:Staphylococcus aureus was shown to transport iron complexed to a variety of hydroxamate type siderophores, including ferrichrome, aerobactin, and desferrioxamine. An S. aureus mutant defective in the ability to transport ferric hydroxamate complexes was isolated from a Tn917-LTV1 transposon insertion library after sel...

journal_title：Journal of bacteriology

authors： Sebulsky MT,Hohnstein D,Hunter MD,Heinrichs DE

更新日期：2000-08-01 00:00:00

abstract：:The effects of bdellovibrio infection on host permeability and respiration were investigated by measuring respiration rates and the rate of o-nitrophenyl-beta-d-galactopyranoside hydrolysis during the course of single infection cycles of Bdellovibrio bacteriovorus strain 109 growing on Escherichia coli ML 35 (lac i(-)...

journal_title：Journal of bacteriology

authors： Rittenberg SC,Shilo M

更新日期：1970-04-01 00:00:00

abstract：:We examined the kinetics of beta-galactosidase (EC 3.2.1.23) induction in the yeast Kluyveromyces lactis. Enzyme activity began to increase 10 to 15 min, about 1/10 of a cell generation, after the addition of inducer and continued to increase linearly for from 7 to 9 cell generations before reaching a maximum, some 12...

journal_title：Journal of bacteriology

authors： Dickson RC,Markin JS

更新日期：1980-06-01 00:00:00

abstract：:The degradation of synthetic compounds requires bacteria to recruit and adapt enzymes from pathways for naturally occurring compounds. Previous work defined the steps in 2,4-dinitrotoluene (2,4-DNT) metabolism through the ring fission reaction. The results presented here characterize subsequent steps in the pathway th...

journal_title：Journal of bacteriology

authors： Johnson GR,Jain RK,Spain JC

更新日期：2002-08-01 00:00:00

abstract：:The response of Salmonella typhimurium to various forms of environmental stress was examined by using O'Farrell two-dimensional gel electrophoresis. Polypeptides (a total of 110) which quantitatively increased during various starvations, anaerobiosis, or heat shock were identified and cataloged in reference to a stand...

journal_title：Journal of bacteriology

authors： Spector MP,Aliabadi Z,Gonzalez T,Foster JW

更新日期：1986-10-01 00:00:00

abstract：:The vir locus of Bordetella pertussis apparently encodes a trans-acting positive regulator that is required for the coordinate expression of genes associated with virulence: pertussis toxin, filamentous hemagglutinin (FHA), hemolysin, and adenylate cyclase toxin. DNA clones of vir and of genes required for the synthes...

journal_title：Journal of bacteriology

authors： Stibitz S,Weiss AA,Falkow S

更新日期：1988-07-01 00:00:00

abstract：:The major coat protein of the bacteriophage f1 is synthesized during infection of Escherichia coli and becomes tightly associated with the host membrane. This synthesis was studied in conjunction with the strain BB26-36, a mutant defective in phospholipid synthesis, to investigate basic questions concerning membrane p...

journal_title：Journal of bacteriology

authors： Cashman JS,Webster RE

更新日期：1977-03-01 00:00:00

abstract：:Wzc(CPS) is a tyrosine autokinase essential for the assembly of a high-molecular-weight (HMW) group 1 capsular polysaccharide (CPS) in Escherichia coli. Homologues of Wzc participate in the formation of CPS and exopolysaccharides in a variety of gram-positive and gram-negative bacteria. Phosphorylation of tyrosine res...

journal_title：Journal of bacteriology

authors： Paiment A,Hocking J,Whitfield C

更新日期：2002-12-01 00:00:00

abstract：:Streptococcus pyogenes, or group A Streptococcus (GAS), is both a pathogen and an asymptomatic colonizer of human hosts and produces a large number of surface-expressed and secreted factors that contribute to a variety of infection outcomes. The GAS-secreted cysteine protease SpeB has been well studied for its effects...

journal_title：Journal of bacteriology

authors： Carothers KE,Liang Z,Mayfield J,Donahue DL,Lee M,Boggess B,Ploplis VA,Castellino FJ,Lee SW

更新日期：2020-05-11 00:00:00

abstract：:The protein motive force of metabolizing Bacillus subtilis cells was only slightly affected by changes in the external pH between 5 and 8, although the electrical component and the chemical component of the proton motive force contributed differently at different external pH. The electrical component of the proton mot...

journal_title：Journal of bacteriology

authors： Shioi JI,Matsuura S,Imae Y

更新日期：1980-12-01 00:00:00

abstract：:Soluble reduced nicotinamide adenine dinucleotide oxidase activity in extracts of Bacillus subtilis spores was stimulated by the addition of not only flavine mononucleotide (FMN) or flavine adenine dinucleotide (FAD) but also alpha-alpha'-dipyridyl or o-phenanthroline. These chelating agents showed stronger effect on ...

journal_title：Journal of bacteriology

authors： Tochikubo K

更新日期：1974-03-01 00:00:00

abstract：:We have recently established that the facultative phototrophic bacterium Rhodobacter sphaeroides, like the closely related Rhodobacter capsulatus species, contains both the previously characterized mobile electron carrier cytochrome c2 (cyt c2) and the more recently discovered membrane-anchored cyt cy. However, R. sph...

journal_title：Journal of bacteriology

authors： Daldal F,Mandaci S,Winterstein C,Myllykallio H,Duyck K,Zannoni D

更新日期：2001-03-01 00:00:00

abstract：:Attaching and effacing (AE) pathogens colonize the gut mucosa using a type three secretion system (T3SS) and a suite of effector proteins. The locus of enterocyte effacement (LEE) is the defining genetic feature of the AE pathogens, encoding the T3SS and the core effector proteins necessary for pathogenesis. Extensive...

journal_title：Journal of bacteriology

authors： Furniss RCD,Clements A

更新日期：2017-12-20 00:00:00

abstract：:Toluene dioxygenase from Pseudomonas putida F1 has been studied extensively with aromatic substrates. The present work examined the toluene dioxygenase-catalyzed oxidation of various halogenated ethenes, propenes, butenes and nonhalogenated cis-2-pentene, an isomeric mix of 2-hexenes, cis-2-heptene, and cis-2-octene a...

journal_title：Journal of bacteriology

authors： Lange CC,Wackett LP

更新日期：1997-06-01 00:00:00

abstract：:Glycosylation is a common modulation of protein function in eukaryotes and is biologically important. However, in bacteria protein glycosylation is rare, and relatively few bacterial glycoproteins are known. In Escherichia coli only two glycoproteins have been described to date. Here we introduce a novel member to thi...

journal_title：Journal of bacteriology

authors： Sherlock O,Dobrindt U,Jensen JB,Munk Vejborg R,Klemm P

更新日期：2006-03-01 00:00:00

abstract：:Birnbaum, Jerome (University of Cincinnati, Cincinnati, Ohio), and Herman C. Lichstein. Metabolism of biotin and analogues of biotin by microorganisms. IV. Degradation of biotin, oxybiotin, and desthiobiotin by Lactobacillus casei. J. Bacteriol. 92:925-930. 1966.-Lactobacillus casei degrades biotin when it is present ...

journal_title：Journal of bacteriology

authors： Birnbaum J,Lichstein HC

更新日期：1966-10-01 00:00:00

abstract：:The lactose-H+ symport protein (LacS) of Streptococcus thermophilus has a carboxyl-terminal regulatory domain (IIALacS) that is homologous to a family of proteins and protein domains of the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) in various organisms, of which IIAGlc of Escherichia coli is the...

journal_title：Journal of bacteriology

authors： Gunnewijk MG,Postma PW,Poolman B

更新日期：1999-01-01 00:00:00

abstract：:A mutant (pgi) of Coprinus macrorhizus deficient in phosphoglucose isomerase did not grow on fructose and grew poorly on glucose. The pgi mutation inhibited the formation of monokaryotic and dikaryotic fruiting bodies. ...

journal_title：Journal of bacteriology

authors： Nyunoya H,Ishikawa T

更新日期：1980-10-01 00:00:00

abstract：:Rickettsia conorii subsp. israelensis is the agent of Israeli spotted fever. The present study reports the draft genome of Rickettsia conorii subsp. israelensis strain ISTT CDC1, isolated from a Rhipicephalus sanguineus tick collected in Israel. ...

journal_title：Journal of bacteriology

authors： Sentausa E,El Karkouri K,Robert C,Raoult D,Fournier PE

更新日期：2012-09-01 00:00:00

abstract：:The ompA gene of Escherichia coli codes for a major protein of the outer membrane. When this gene was moved between various unrelated strains (E. coli K-12 and two clinical isolates of E. coli) by transduction, the gene was expressed very poorly. Recombinants carrying "foreign" genes produced no OmpA protein which cou...

journal_title：Journal of bacteriology

authors： Beher M,Pugsley A,Schnaitman C

更新日期：1980-07-01 00:00:00

abstract：:Of 16 restriction endonucleases known to hydrolyze rare 6- or 8-base recognition sequences that were tested, only SpeI, NotI, AscI, and SfiI generated fragments of chromosomal DNA from Yersinia pestis, the causative agent of bubonic plague, of sufficient length to permit physical analysis by pulsed-field gel electroph...

journal_title：Journal of bacteriology

authors： Lucier TS,Brubaker RR

更新日期：1992-04-01 00:00:00

abstract：:Spheroplasts of two different auxotrophic strains of Saccharomyces cerevisiae, both of mating type a, were fused with the aid of polyethylene glycol and calcium ions. After reversion to vegetative cells in solid media, the resulting zygotes were shown to be diploid cells of mating type aa. ...

journal_title：Journal of bacteriology

authors： van Solingen P,van der Plaat JB

更新日期：1977-05-01 00:00:00

abstract：:Preparations of membrane plus wall derived from Bacillus subtilis W23 were used to study the in vitro synthesis of peptidoglycan and teichoic acid and their linkage to the preexisting cell wall. The teichoic acid synthesis showed an ordered requirement for the incorporation of N-acetylglucosamine from uridine 5'-dipho...

journal_title：Journal of bacteriology

authors： Wyke AW,Ward JB

更新日期：1977-06-01 00:00:00

abstract：:The biological activity of Odontomyces viscosus, which has been reported to cause periodontal disease in hamsters, was examined. The microorganism was cultured anaerobically in Brain Heart Infusion broth, and the cells were harvested. The washed cells were injected intradermally into the abdomen of rabbits. After 72 h...

journal_title：Journal of bacteriology

authors： Schuster GS,Hayashi JA,Bahn AN

更新日期：1967-01-01 00:00:00