Redox signal transduction by the ArcB sensor kinase of Haemophilus influenzae lacking the PAS domain.

Abstract:

:The Arc (anoxic redox control) two-component signal transduction system of Escherichia coli, which comprises the tripartite ArcB sensor kinase and the ArcA response regulator, modulates the expression of numerous operons in response to redox conditions of growth. We demonstrate that the arcA and arcB genes of Haemophilus influenzae specify a two-component system. The Arc proteins of the two bacterial species sufficiently resemble each other that they can participate in heterologous transphosphorylation in vitro. Moreover, the Arc system of H. influenzae mediates transcriptional control according to the redox condition of growth both autologously in its own host and homologously in E. coli, indicating a high degree of functional conservation of the signal transduction system. The H. influenzae ArcB, however, lacks the PAS domain present in the region of E. coli ArcB linking the transmembrane to the cytosolic catalytic domains. Because the PAS domain participates in signal reception in a variety of sensory proteins, including sensors of molecular oxygen and redox state, a similar role was previously ascribed to it in ArcB. Our results demonstrate that the ArcB protein of H. influenzae mediates signal transduction in response to redox conditions of growth despite the absence of the PAS domain.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Georgellis D,Kwon O,Lin EC,Wong SM,Akerley BJ

doi

10.1128/JB.183.24.7206-7212.2001

keywords:

subject

Has Abstract

pub_date

2001-12-01 00:00:00

pages

7206-12

issue

24

eissn

0021-9193

issn

1098-5530

journal_volume

183

pub_type

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