Effect of perturbation of ATP level on the activity and regulation of nitrogenase in Rhodospirillum rubrum.

Abstract:

:Nitrogenase activity in Rhodospirillum rubrum and in some other photosynthetic bacteria is regulated in part by the availability of light. This regulation is through a posttranslational modification system that is itself regulated by P(II) homologs in the cell. P(II) is one of the most broadly distributed regulatory proteins in nature and directly or indirectly senses nitrogen and carbon signals in the cell. However, its possible role in responding to light availability remains unclear. Because P(II) binds ATP, we tested the hypothesis that removal of light would affect P(II) by changing intracellular ATP levels, and this in turn would affect the regulation of nitrogenase activity. This in vivo test involved a variety of different methods for the measurement of ATP, as well as the deliberate perturbation of intracellular ATP levels by chemical and genetic means. To our surprise, we found fairly normal levels of nitrogenase activity and posttranslational regulation of nitrogenase even under conditions of drastically reduced ATP levels. This indicates that low ATP levels have no more than a modest impact on the P(II)-mediated regulation of NifA activity and on the posttranslational regulation of nitrogenase activity. The relatively high nitrogenase activity also shows that the ATP-dependent electron flux from dinitrogenase reductase to dinitrogenase is also surprisingly insensitive to a depleted ATP level. These in vivo results disprove the simple model of ATP as the key energy signal to P(II) under these conditions. We currently suppose that the ratio of ADP/ATP might be the relevant signal, as suggested by a number of recent in vitro analyses.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Zhang Y,Pohlmann EL,Roberts GP

doi

10.1128/JB.00585-09

subject

Has Abstract

pub_date

2009-09-01 00:00:00

pages

5526-37

issue

17

eissn

0021-9193

issn

1098-5530

pii

JB.00585-09

journal_volume

191

pub_type

杂志文章
  • Biosynthesis of 4-aminobenzoate in Escherichia coli.

    abstract::Two different mutations (pabA and pabB) affecting 4-aminobenzoate biosynthesis were obtained in strains of Escherichia coli lacking chorismate mutase and anthranilate synthetase activity, thus allowing study of the pathway of biosynthesis of 4-aminobenzoate by use of cell extracts of strains carrying the pab mutations...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.102.3.767-773.1970

    authors: Huang M,Gibson F

    更新日期:1970-06-01 00:00:00

  • Regulation of the potassium to sodium ratio and of the osmotic potential in relation to salt tolerance in yeasts.

    abstract::By using the isotope pairs (22)Na-(24)Na and (42)K-(86)Rb, the uptake and retention of Na and K was studied in the salt-tolerant Debaryomyces hansenii and in the less tolerant Saccharomyces cerevisiae at NaCl levels of 4 mm and 0.68, 1.35, and 2.7 m in the medium. The ratio of K to Na is much higher in the cells than ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.2.836-845.1969

    authors: Norkrans B,Kylin A

    更新日期:1969-11-01 00:00:00

  • Carrier-mediated transport of folate in a mutant of Pediococcus cerevisiae.

    abstract::A mutant strain of Pediococcus cerevisiae (P. cerevisiae/PteGlu) was isolated which grows on low-folate (PteGlu) concentrations (200 pg/ml). The growth response of the parent and mutant strains to folinate (5-CHO-H(4)PteGlu) was the same. The transport of (14)C-PteGlu by P. cerevisiae/PteGlu was temperature-dependent ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.114.2.485-490.1973

    authors: Mandelbaum-Shavit F,Grossowicz N

    更新日期:1973-05-01 00:00:00

  • Iron-dependent cytochrome c1 expression is mediated by the status of heme in Bradyrhizobium japonicum.

    abstract::The heme prosthetic group of heme proteins contains iron, which can be a limiting nutrient. Here, we show that cytochrome c1 protein from Bradyrhizobium japonicum was strongly affected by the iron status, with low expression in cells grown under iron limitation. This control was not affected in mutants encoding the ir...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.15.5084-5089.2005

    authors: Gao T,O'Brian MR

    更新日期:2005-08-01 00:00:00

  • Role of the ganSPQAB Operon in Degradation of Galactan by Bacillus subtilis.

    abstract::Bacillus subtilis possesses different enzymes for the utilization of plant cell wall polysaccharides. This includes a gene cluster containing galactan degradation genes (ganA and ganB), two transporter component genes (ganQ and ganP), and the sugar-binding lipoprotein-encoding gene ganS (previously known as cycB). The...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00468-16

    authors: Watzlawick H,Morabbi Heravi K,Altenbuchner J

    更新日期:2016-09-22 00:00:00

  • Regulation of amino acid transport in Escherichia coli by transcription termination factor rho.

    abstract::Amino acid transport rates and amino acid binding proteins were examined in a strain containing the rho-120 mutation (formerly SuA), which has been shown to lower the rho-dependent, ribonucleic acid-activated adenosine triphosphatase activity to 9% of the rho activity in the isogenic wild-type strain. Tryptophan and p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.130.3.1024-1029.1977

    authors: Quay SC,Oxender DL

    更新日期:1977-06-01 00:00:00

  • Identification of a plasmid-borne locus in Rhizobium etli KIM5s involved in lipopolysaccharide O-chain biosynthesis and nodulation of Phaseolus vulgaris.

    abstract::Screening of derivatives of Rhizobium etli KIM5s randomly mutagenized with mTn5SSgusA30 resulted in the identification of strain KIM-G1. Its rough colony appearance, flocculation in liquid culture, and Ndv(-) Fix(-) phenotype were indicative of a lipopolysaccharide (LPS) defect. Electrophoretic analysis of cell-associ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.18.5606-5614.1999

    authors: Vinuesa P,Reuhs BL,Breton C,Werner D

    更新日期:1999-09-01 00:00:00

  • Rhodobacter sphaeroides WS8 expresses a polypeptide that is similar to MotB of Escherichia coli.

    abstract::A gene which complements a paralyzed flagellar mutant of Rhodobacter sphaeroides was sequenced. The derived protein sequence has similarity to MotB. R. sphaeroides MotB lacks the C-terminal peptidoglycan-binding motif of other MotB proteins. This divergence of sequence may reflect the unusual, unidirectional, stop-sta...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.10.2929-2932.1995

    authors: Shah DS,Armitage JP,Sockett RE

    更新日期:1995-05-01 00:00:00

  • Aspartate aminotransferase activity is required for aspartate catabolism and symbiotic nitrogen fixation in Rhizobium meliloti.

    abstract::A mutant of Rhizobium meliloti, 4R3, which is unable to grow on aspartate has been isolated. The defect is specific to aspartate utilization, since 4R3 is not an auxotroph and grows as well as its parent strain on other carbon and nitrogen sources. The defect was correlated with an inability to fix nitrogen within nod...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.9.2879-2887.1991

    authors: Rastogi VK,Watson RJ

    更新日期:1991-05-01 00:00:00

  • Proteolytic Cleavage of the Immunodominant Outer Membrane Protein rOmpA in Rickettsia rickettsii.

    abstract::Rickettsia rickettsii, the causative agent of Rocky Mountain spotted fever, contains two immunodominant proteins, rOmpA and rOmpB, in the outer membrane. Both rOmpA and rOmpB are conserved throughout spotted fever group rickettsiae as members of a family of autotransporter proteins. Previously, it was demonstrated tha...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00826-16

    authors: Noriea NF,Clark TR,Mead D,Hackstadt T

    更新日期:2017-02-28 00:00:00

  • Escherichia coli has two homologous glutamate decarboxylase genes that map to distinct loci.

    abstract::Degenerate oligonucleotides based on the published Escherichia coli glutamate decarboxylase (GAD) protein sequence were used in a polymerase chain reaction to generate a DNA probe for the E. coli GAD structural gene. Southern blots showed that there were two cross-hybridizing GAD genes, and both of these were cloned a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.18.5820-5826.1992

    authors: Smith DK,Kassam T,Singh B,Elliott JF

    更新日期:1992-09-01 00:00:00

  • Transcriptional regulation of the heterocyst patterning gene patA from Anabaena sp. strain PCC 7120.

    abstract::The filamentous cyanobacterium Anabaena sp. strain PCC 7120 forms a periodic pattern of nitrogen-fixing heterocysts when grown in the absence of combined nitrogen. PatA is necessary for proper patterning of heterocysts along filaments. In this study, apparent transcriptional start points (tsps) were identified at nucl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00577-10

    authors: Young-Robbins SS,Risser DD,Moran JR,Haselkorn R,Callahan SM

    更新日期:2010-09-01 00:00:00

  • Residual polarity and transcription-translation coupling during recovery from chloramphenicol or fusidic acid.

    abstract::Fusidic acid or chloramphenicol was used to inhibit peptide synthesis to 1% of normal in Escherichia coli B, strain AS19. After 10 min of inhibition, peptide synthesis could be quickly restored to 80% of the normal rate after washing the bacteria on a filter. However, even in the presence of adenosine 3'-5'-cyclic-mon...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.117.2.631-640.1974

    authors: Pastushok C,Kennell D

    更新日期:1974-02-01 00:00:00

  • Mutations in ampG or ampD affect peptidoglycan fragment release from Neisseria gonorrhoeae.

    abstract::Neisseria gonorrhoeae releases peptidoglycan fragments during growth. The majority of the fragments released are peptidoglycan monomers, molecules known to increase pathogenesis through the induction of proinflammatory cytokines and responsible for the killing of ciliated epithelial cells. In other gram-negative bacte...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01194-07

    authors: Garcia DL,Dillard JP

    更新日期:2008-06-01 00:00:00

  • Transcriptome analysis of the progressive adaptation of Lactococcus lactis to carbon starvation.

    abstract::Adaptation of Lactococcus lactis towards progressive carbon starvation is mediated by three different types of transcriptomic responses: (i) global responses, i.e., general decreases of functions linked to bacterial growth and lack of induction of the general stress response; (ii) specific responses functionally relat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.10.3589-3592.2005

    authors: Redon E,Loubiere P,Cocaign-Bousquet M

    更新日期:2005-05-01 00:00:00

  • DNA binding: a novel function of Pseudomonas aeruginosa type IV pili.

    abstract::The opportunistic pathogen Pseudomonas aeruginosa produces multifunctional, polar, filamentous appendages termed type IV pili. Type IV pili are involved in colonization during infection, twitching motility, biofilm formation, bacteriophage infection, and natural transformation. Electrostatic surface analysis of modele...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.4.1455-1464.2005

    authors: van Schaik EJ,Giltner CL,Audette GF,Keizer DW,Bautista DL,Slupsky CM,Sykes BD,Irvin RT

    更新日期:2005-02-01 00:00:00

  • Biosynthesis of riboflavin: characterization of the bifunctional deaminase-reductase of Escherichia coli and Bacillus subtilis.

    abstract::The ribG gene at the 5' end of the riboflavin operon of Bacillus subtilis and a reading frame at 442 kb on the Escherichia coli chromosome (subsequently designated ribD) show similarity with deoxycytidylate deaminase and with the RIB7 gene of Saccharomyces cerevisiae. The ribG gene of B. subtilis and the ribD gene of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.6.2022-2028.1997

    authors: Richter G,Fischer M,Krieger C,Eberhardt S,Lüttgen H,Gerstenschläger I,Bacher A

    更新日期:1997-03-01 00:00:00

  • ftsZ is an essential cell division gene in Escherichia coli.

    abstract::The ftsZ gene is thought to be an essential cell division gene in Escherichia coli. We constructed a null allele of ftsZ in a strain carrying additional copies of ftsZ on a plasmid with a temperature-sensitive replication defect. This strain was temperature sensitive for cell division and viability, confirming that ft...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.11.3500-3506.1991

    authors: Dai K,Lutkenhaus J

    更新日期:1991-06-01 00:00:00

  • Interactions between the lipoprotein PilP and the secretin PilQ in Neisseria meningitidis.

    abstract::Neisseria meningitidis can be the causative agent of meningitis or septicemia. This bacterium expresses type IV pili, which mediate a variety of functions, including autoagglutination, twitching motility, biofilm formation, adherence, and DNA uptake during transformation. The secretin PilQ supports type IV pilus extru...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00060-07

    authors: Balasingham SV,Collins RF,Assalkhou R,Homberset H,Frye SA,Derrick JP,Tønjum T

    更新日期:2007-08-01 00:00:00

  • Differential recognition of surface proteins in Streptococcus pyogenes by two sortase gene homologs.

    abstract::The interaction of Streptococcus pyogenes (group A streptococcus [GAS]) with its human host requires several surface proteins. In this study, we isolated mutations in a gene required for the surface localization of protein F by transposon mutagenesis of the M6 strain JRS4. This gene (srtA) encodes a protein homologous...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.8.2181-2191.2002

    authors: Barnett TC,Scott JR

    更新日期:2002-04-01 00:00:00

  • Characterization of Pneumocystis carinii PHR1, a pH-regulated gene important for cell wall Integrity.

    abstract::Pneumocystis carinii remains an important opportunistic fungal pathogen causing life-threatening pneumonia in patients with AIDS and malignancy. Currently, little is known about how the organism adapts to environmental stresses and maintains its cellular integrity. We recently discovered an open reading frame approxim...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.23.6740-6745.2001

    authors: Kottom TJ,Thomas CF Jr,Limper AH

    更新日期:2001-12-01 00:00:00

  • Characterization of the hcnABC gene cluster encoding hydrogen cyanide synthase and anaerobic regulation by ANR in the strictly aerobic biocontrol agent Pseudomonas fluorescens CHA0.

    abstract::The secondary metabolite hydrogen cyanide (HCN) is produced by Pseudomonas fluorescens from glycine, essentially under microaerophilic conditions. The genetic basis of HCN synthesis in P. fluorescens CHA0 was investigated. The contiguous structural genes hcnABC encoding HCN synthase were expressed from the T7 promoter...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.12.3187-3196.1998

    authors: Laville J,Blumer C,Von Schroetter C,Gaia V,Défago G,Keel C,Haas D

    更新日期:1998-06-01 00:00:00

  • Protoplast transformation of glutamate-producing bacteria with plasmid DNA.

    abstract::A method for polyethylene glycol-induced protoplast transformation of glutamate-producing bacteria with plasmid DNA was established. Protoplasts were prepared from cells grown in the presence of penicillin by treatment with lysozyme in a hypertonic medium. The concentration of penicillin during growth affected the eff...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.159.1.306-311.1984

    authors: Katsumata R,Ozaki A,Oka T,Furuya A

    更新日期:1984-07-01 00:00:00

  • How a Genetically Stable Extremophile Evolves: Modes of Genome Diversification in the Archaeon Sulfolobus acidocaldarius.

    abstract::In order to analyze in molecular terms how Sulfolobus genomes diverge, damage-induced mutations and natural polymorphisms (PMs) were identified in laboratory constructs and wild-type isolates, respectively, of Sulfolobus acidocaldarius Among wild-type isolates drawn from one local population, pairwise nucleotide diver...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00177-17

    authors: Mao D,Grogan DW

    更新日期:2017-08-08 00:00:00

  • Biosynthesis of the outer membrane receptor for vitamin B12, E colicins, and bacteriophage BF23 by Escherichia coli: kinetics of phenotypic expression after the introduction of bfe+ and bfe alleles.

    abstract::The bfe locus codes for the cell surface receptor for vitamin B12, the E colicins, and bacteriophage BF23 in the Escherichia coli outer membrane. When the bfe+ allele, which is closely linked to the argH locus, was introduced into an argH bfe recipient by conjugation, arg+ recombinant cells rapidly and simultaneously ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.129.1.265-275.1977

    authors: Bassford PJ Jr,Kadner RJ,Schnaitman CA

    更新日期:1977-01-01 00:00:00

  • Fluorescence and viability of Proteus mirabilis stained directly with fluorescein isothiocyanate.

    abstract::Grunberg, E. (Hoffman-La Roche, Inc., Nutley, N.J.), and R. Cleeland. Fluorescence and viability of Proteus mirabilis stained directly with fluorescein isothiocyanate. J. Bacteriol. 92:23-27. 1966.-Washed cell suspensions of Proteus mirabilis, under the proper conditions, stained well with fluorescein isothiocyanate w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.92.1.23-27.1966

    authors: Grunberg E,Cleeland R

    更新日期:1966-07-01 00:00:00

  • Temperature regulation of heat-labile enterotoxin (LT) synthesis in Escherichia coli is mediated by an interaction of H-NS protein with the LT A-subunit DNA.

    abstract::Protein and mRNA levels of heat-labile enterotoxin (LT) of Escherichia coli are highest at 37 degrees C, and they decrease gradually as temperature is decreased. This temperature effect is eliminated in an Hns- mutant. Deletion of portions of DNA coding for the LT A subunit also results in an increase in LT expression...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.14.3715-3718.1998

    authors: Trachman JD,Maas WK

    更新日期:1998-07-01 00:00:00

  • New aerobic benzoate oxidation pathway via benzoyl-coenzyme A and 3-hydroxybenzoyl-coenzyme A in a denitrifying Pseudomonas sp.

    abstract::A denitrifying Pseudomonas sp. is able to oxidize aromatic compounds compounds completely to CO2, both aerobically and anaerobically. It is shown that benzoate is aerobically oxidized by a new degradation pathway via benzoyl-coenzyme A (CoA) and 3-hydroxybenzoyl-CoA. The organism grew aerobically with benzoate, 3-hydr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.15.4851-4858.1993

    authors: Altenschmidt U,Oswald B,Steiner E,Herrmann H,Fuchs G

    更新日期:1993-08-01 00:00:00

  • Localization of the cis-acting regulatory DNA sequences of the Myxococcus xanthus tps and ops genes.

    abstract::The cis-acting regulatory regions of the tps and ops genes of Myxococcus xanthus were localized by analyzing the expression of fusions of these genes with the lacZ gene. A 201-base-pair (bp) fragment of tps DNa extending 95 bp upstream (-95) from the transcriptional start was sufficient to direct developmentally regul...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.10.4931-4938.1988

    authors: Downard JS,Kim SH,Kil KS

    更新日期:1988-10-01 00:00:00

  • Analysis of the novel benzylsuccinate synthase reaction for anaerobic toluene activation based on structural studies of the product.

    abstract::Recent studies of anaerobic toluene catabolism have demonstrated a novel reaction for anaerobic hydrocarbon activation: the addition of the methyl carbon of toluene to fumarate to form benzylsuccinate. In vitro studies of the anaerobic benzylsuccinate synthase reaction indicate that the H atom abstracted from the tolu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.20.5454-5457.1998

    authors: Beller HR,Spormann AM

    更新日期:1998-10-01 00:00:00