Analysis of the novel benzylsuccinate synthase reaction for anaerobic toluene activation based on structural studies of the product.

abstract：:Pelosinus fermentans 16S rRNA gene sequences have been reported from diverse geographical sites since the recent isolation of the type strain. We present the genome sequence of the P. fermentans type strain R7 (DSM 17108) and genome sequences for two new strains with different abilities to reduce iron, chromate, and u...

journal_title：Journal of bacteriology

authors： Brown SD,Podar M,Klingeman DM,Johnson CM,Yang ZK,Utturkar SM,Land ML,Mosher JJ,Hurt RA Jr,Phelps TJ,Palumbo AV,Arkin AP,Hazen TC,Elias DA

更新日期：2012-09-01 00:00:00

abstract：:Promoters were cloned from the chromosomal DNA of Lactococcus lactis subsp. lactis NP4510 by using promoter-probe vector pGKV210. N-Methyl-N'-nitro-N-nitrosoguanidine-induced mutagenesis of L. lactis-(pBV413), with low-level expression of the cat-86 gene, resulted in enhanced expression. Subcloning and sequencing of t...

journal_title：Journal of bacteriology

authors： Bojovic B,Djordjevic G,Banina A,Topisirovic L

更新日期：1994-11-01 00:00:00

abstract：:A cloned cytolytic determinant from the genome of Bacillus cereus GP-4 has been characterized at the molecular level. Nucleotide sequence determination revealed the presence of two open reading frames. Both open reading frames were found by deletion and complementation analysis to be necessary for expression of the he...

journal_title：Journal of bacteriology

authors： Gilmore MS,Cruz-Rodz AL,Leimeister-Wächter M,Kreft J,Goebel W

更新日期：1989-02-01 00:00:00

abstract：:We present the nucleotide sequence of the oriT region from plasmid R100. Comparison to other IncF plasmids revealed homology around the proposed nick sites as well as conservation of inverted repeated sequences in the nonhomologous region. Three areas showed strong homology (eight of nine nucleotides) to the consensus...

journal_title：Journal of bacteriology

authors： McIntire SA,Dempsey WB

更新日期：1987-08-01 00:00:00

abstract：:In this study, the H303A mutant form of the cbb(3) oxidase (H303A oxidase), which has the H303A mutation in its catalytic subunit (CcoN), was purified from Rhodobacter sphaeroides. The H303A oxidase showed the same catalytic activity as did the wild-type form of the oxidase (WT oxidase). The heme contents of the mutan...

journal_title：Journal of bacteriology

authors： Kim YJ,Ko IJ,Lee JM,Kang HY,Kim YM,Kaplan S,Oh JI

更新日期：2007-08-01 00:00:00

abstract：:Enhancement of the production of soluble recombinant penicillin acylase in Escherichia coli via coexpression of a periplasmic protease/chaperone, DegP, was demonstrated. Coexpression of DegP resulted in a shift of in vivo penicillin acylase (PAC) synthesis flux from the nonproductive pathway to the productive one when...

journal_title：Journal of bacteriology

authors： Pan KL,Hsiao HC,Weng CL,Wu MS,Chou CP

更新日期：2003-05-01 00:00:00

abstract：:Streptomyces clavuligerus produces at least five different clavam metabolites, including clavulanic acid and the methionine antimetabolite, alanylclavam. In vitro transposon mutagenesis was used to analyze a 13-kb region upstream of the known paralogue gene cluster. The paralogue cluster includes one group of clavulan...

journal_title：Journal of bacteriology

authors： Zelyas NJ,Cai H,Kwong T,Jensen SE

更新日期：2008-12-01 00:00:00

abstract：:2-Aminonumconic 6-semialdehyde is an unstable intermediate in the biodegradation of nitrobenzene and 2-aminophenol by Pseudomonas pseudoalcaligenes JS45. Previous work has shown that enzymes in cell extracts convert 2-aminophenol to 2-aminomuconate in the presence of NAD+. In the present work, 2-aminomuconic semialdeh...

journal_title：Journal of bacteriology

authors： He Z,Davis JK,Spain JC

更新日期：1998-09-01 00:00:00

abstract：:Electron cryotomography was used to analyze the structure of the Lyme disease spirochete, Borrelia burgdorferi. This methodology offers a new means for studying the native architecture of bacteria by eliminating the chemical fixing, dehydration, and staining steps of conventional electron microscopy. Using electron cr...

journal_title：Journal of bacteriology

authors： Charon NW,Goldstein SF,Marko M,Hsieh C,Gebhardt LL,Motaleb MA,Wolgemuth CW,Limberger RJ,Rowe N

更新日期：2009-01-01 00:00:00

abstract：:The DosS (DevS) and DosT histidine kinases form a two-component system together with the DosR (DevR) response regulator in Mycobacterium tuberculosis. DosS and DosT, which have high sequence similarity to each other over the length of their amino acid sequences, contain two GAF domains (GAF-A and GAF-B) in their N-ter...

journal_title：Journal of bacteriology

authors： Kim MJ,Park KJ,Ko IJ,Kim YM,Oh JI

更新日期：2010-10-01 00:00:00

abstract：:Glucose-1-(14)C and acetylglucosamine-1-(14)C were added singly and together with equal amounts of the unlabeled reciprocal to Brain Heart Infusion and used for the culture of Streptococcus pyogenes. The labeling pattern of the rhamnose, glucosamine, and muramic acid in the cell wall supported an intermediary role for...

journal_title：Journal of bacteriology

authors： Barkulis SS,Boltralik JJ,Hankin H,Heymann H

更新日期：1967-10-01 00:00:00

abstract：:The mechanisms involved in the virulence of Yersinia pestis, the plague pathogen, are not fully understood. In previous research, we found that a Y. pestis mutant lacking the HicB3 (YPO3369) putative orphan antitoxin was attenuated for virulence in a murine model of bubonic plague. Toxin-antitoxin systems (TASs) are w...

journal_title：Journal of bacteriology

authors： Bibi-Triki S,Li de la Sierra-Gallay I,Lazar N,Leroy A,Van Tilbeurgh H,Sebbane F,Pradel E

更新日期：2014-11-01 00:00:00

abstract：:The reactive alpha-oxoaldehydes such as glyoxal (GO) and methylglyoxal (MG) are generated in vivo from sugars through oxidative stress. GO and MG are believed to be removed from cells by glutathione-dependent glyoxalases and other aldehyde reductases. We isolated a number of GO-resistant (GO(r)) mutants from Escherich...

journal_title：Journal of bacteriology

authors： Lee C,Kim I,Lee J,Lee KL,Min B,Park C

更新日期：2010-08-01 00:00:00

abstract：UNLABELLED:To stabilize cellular integrity in the face of environmental perturbations, most bacteria, including cyanobacteria, synthesize and maintain a strong, flexible, three-dimensional peptidoglycan lattice. Anabaena sp. strain PCC 7120 is a filamentous cyanobacterium capable of differentiating morphologically dist...

journal_title：Journal of bacteriology

authors： Videau P,Rivers OS,Ushijima B,Oshiro RT,Kim MJ,Philmus B,Cozy LM

更新日期：2016-03-31 00:00:00

abstract：:Evidence for the existence of two molecular species of exfoliative toxin (ET) synthesized by Staphylococcus hyicus (SHET) under chromosomal and plasmid control is presented. Serological evidence that these molecular species of toxins are distinct from each other is given. The molecular weights of SHET from plasmidless...

journal_title：Journal of bacteriology

authors： Sato H,Watanabe T,Higuchi K,Teruya K,Ohtake A,Murata Y,Saito H,Aizawa C,Danbara H,Maehara N

更新日期：2000-07-01 00:00:00

abstract：:Flagellar motility is an important determinant of Campylobacter jejuni that is required for promoting interactions with various hosts to promote gastroenteritis in humans or commensal colonization of many animals. In a previous study, we identified a nonmotile mutant of C. jejuni 81-176 with a transposon insertion in ...

journal_title：Journal of bacteriology

authors： Sommerlad SM,Hendrixson DR

更新日期：2007-01-01 00:00:00

abstract：:During sporulation, the Bacillus subtilis transcription factor sigmaK is activated by regulated proteolytic processing. I have used a system that facilitates the analysis of the contributions of a modified form of the processing enzyme, SpoIVFB-GFP, and the regulatory proteins BofA and SpoIVFA to the conversion of pro...

journal_title：Journal of bacteriology

authors： Resnekov O

更新日期：1999-09-01 00:00:00

abstract：:Whole-genome sequence analysis of Mycobacterium leprae has revealed a limited number of protein-coding genes, with half of the genome composed of pseudogenes and noncoding regions. We previously showed that some M. leprae pseudogenes are transcribed at high levels and that their expression levels change following infe...

journal_title：Journal of bacteriology

authors： Akama T,Suzuki K,Tanigawa K,Kawashima A,Wu H,Nakata N,Osana Y,Sakakibara Y,Ishii N

更新日期：2009-05-01 00:00:00

abstract：:The Escherichia coli rluD gene encodes a pseudouridine synthase responsible for the pseudouridine (Ψ) modifications at positions 1911, 1915, and 1917 in helix 69 of 23S rRNA. It has been reported that deletion of rluD in K-12 strains of E. coli is associated with extremely slow growth, increased readthrough of stop co...

journal_title：Journal of bacteriology

authors： O'Connor M,Gregory ST

更新日期：2011-01-01 00:00:00

abstract：:A 16-kb BamHI fragment of the lactose plasmid pNZ63 from Leuconostoc lactis NZ6009 was cloned in Escherichia coli MC1061 by using pACYC184 and was found to express a functional beta-galactosidase. Deletion and complementation analysis showed that the coding region for beta-galactosidase was located on a 5.8-kb SalI-Ba...

journal_title：Journal of bacteriology

authors： David S,Stevens H,van Riel M,Simons G,de Vos WM

更新日期：1992-07-01 00:00:00

abstract：:Growth of Agrobacterium tumefaciens on d-glucuronic acid (GlcUA) or d-galacturonic acid (GalUA) induces formation of hexuronic acid dehydrogenase [d-aldohexuronic acid: nicotinamide adenine dinucleotide (NAD) oxidoreductase]. The dehydrogenase, which irreversibly converts GlcUA or GalUA to the corresponding hexaric ac...

journal_title：Journal of bacteriology

authors： Chang YF,Feingold DS

更新日期：1969-09-01 00:00:00

abstract：:The glnA mRNA, encoding glutamine synthetase, is differentially accumulated in the cyanobacterium Synechococcus sp. strain PCC 7942 in media containing different nitrogen sources. With the different nitrogen compounds, transcription of glnA initiated at a single site located -146 nucleotides upstream of the translatio...

journal_title：Journal of bacteriology

authors： Cohen-Kupiec R,Gurevitz M,Zilberstein A

更新日期：1993-12-01 00:00:00

abstract：:A simple three-step procedure is described which yields electrophoretically homogeneous preparations of ribitol-5-phosphate dehydrogenase and xylitol-5-phosphate dehydrogenase. The former enzyme is a 115,000-molecular-weight protein composed of two subunits of identical size and is specific for its substrate, ribitol....

journal_title：Journal of bacteriology

authors： Hausman SZ,London J

更新日期：1987-04-01 00:00:00

abstract：:Escherichia coli outer membranes were prepared by centrifugation to equilibrium in sucrose gradients and then treated with Sarkosyl in the presence of ethylenediaminetetraacetate. The polypeptide profiles of the two outer membrane preparations were compared by two-dimensional polyacrylamide gel electrophoresis. The pa...

journal_title：Journal of bacteriology

authors： Chopra I,Shales SW

更新日期：1980-10-01 00:00:00

abstract：:Streptococcus oralis, a commensal species of the human oral cavity, belongs to the Mitis group of streptococci, which includes one of the major human pathogens as well, S. pneumoniae. We report here the first complete genome sequence of this species. S. oralis Uo5, a high-level penicillin- and multiple-antibiotic-resi...

journal_title：Journal of bacteriology

authors： Reichmann P,Nuhn M,Denapaite D,Brückner R,Henrich B,Maurer P,Rieger M,Klages S,Reinhard R,Hakenbeck R

更新日期：2011-06-01 00:00:00

abstract：:Many microbes coexist within biofilms, or multispecies communities of cells encased in an extracellular matrix. However, little is known about the microbe-microbe interactions relevant for creating these structures. In this study, we explored a striking dual-species biofilm between Bacillus subtilis and Pantoea agglom...

journal_title：Journal of bacteriology

authors： Yannarell SM,Grandchamp GM,Chen SY,Daniels KE,Shank EA

更新日期：2019-08-22 00:00:00

abstract：:Transcriptional regulation was demonstrated in Rickettsia prowazekii, an obligate intracytoplasmic bacterium. The level of citrate synthase (gltA) mRNA II, from promoter P2, was greater in the total RNA isolated from heavily infected L929 cells than in moderately infected L929 cells; conversely, the level of ATP/ADP t...

journal_title：Journal of bacteriology

authors： Cai J,Winkler HH

更新日期：1996-09-01 00:00:00

abstract：:A mutation in Escherichia coli leads to the loss of ribosomal protein L24, severely impaired growth, and a temperature-sensitive phenotype. The mutation was shown to be in rplX, the gene for protein L24, and was due to the alteration of an AAA codon to a TAA stop codon at position 61 in rplX that resulted in a 20-amin...

journal_title：Journal of bacteriology

authors： Nishi K,Dabbs ER,Schnier J

更新日期：1985-09-01 00:00:00

abstract：:Transcriptional regulation of the Mg(2+)-citrate transporter, CitM, the main citrate uptake system of Bacillus subtilis, was studied during growth in rich medium. Citrate in the growth medium was required for induction under all growth conditions. In Luria-Bertani medium containing citrate, citM expression was complet...

journal_title：Journal of bacteriology

authors： Warner JB,Magni C,Lolkema JS

更新日期：2003-02-01 00:00:00

abstract：:The gut microbiota plays a crucial role in the development of the immune system and confers benefits or disease susceptibility to the host. Emerging studies have indicated the gut microbiota could affect pulmonary health and disease through cross talk between the gut microbiota and the lungs. Gut microbiota dysbiosis c...

journal_title：Journal of bacteriology

authors： Zhou A,Lei Y,Tang L,Hu S,Yang M,Wu L,Yang S,Tang B

更新日期：2021-01-25 00:00:00