Chromosomal and extrachromosomal synthesis of exfoliative toxin from Staphylococcus hyicus.

Abstract:

:Evidence for the existence of two molecular species of exfoliative toxin (ET) synthesized by Staphylococcus hyicus (SHET) under chromosomal and plasmid control is presented. Serological evidence that these molecular species of toxins are distinct from each other is given. The molecular weights of SHET from plasmidless strain P-1 (SHETA) and from plasmid-carrying strains P-10 and P-23 (SHETB) were almost equal. Both of the serotypes of SHET exhibited exfoliation in 1-day-old chickens. The plasmid-cured (P(-)) substrains (P-23C1 and P-23C2) of S. hyicus P-23 did not cause exfoliation in 1-day-old chickens, whereas P(-) substrains (P-10C1 and P-10C2) of strain P-10 caused exfoliation, but they decreased their exfoliative activity. These findings suggest that SHETB was synthesized along with SHETA by strain P-10, whereas the P-23 strain synthesized SHETB alone. The plasmid-carrying strain (P-23) as well as the plasmidless strain (P-1) exhibited the typical clinical signs of exudative epidermitis in pigs. However, plasmid-cured (P(-)) substrains of P-23 (P23C1 and P23C2) did not exhibit the typical clinical signs of exudative epidermitis. These findings suggest that SHETA is synthesized under chromosomal control and SHETB is synthesized under plasmid control and that SHET-producing strains can be divided into three groups: SHETA-producing strains, SHETB-producing strains, and strains producing both toxins.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Sato H,Watanabe T,Higuchi K,Teruya K,Ohtake A,Murata Y,Saito H,Aizawa C,Danbara H,Maehara N

doi

10.1128/jb.182.14.4096-4100.2000

keywords:

subject

Has Abstract

pub_date

2000-07-01 00:00:00

pages

4096-100

issue

14

eissn

0021-9193

issn

1098-5530

journal_volume

182

pub_type

杂志文章
  • Transfer of Rhizobium meliloti pSym genes into Agrobacterium tumefaciens: host-specific nodulation by atypical infection.

    abstract::The pSym megaplasmid of Rhizobium meliloti 2011 mobilized by plasmid RP4, or plasmid pGMI42, an RP4-prime derivative which carries a 290-kilobase pSym fragment including nitrogenase and nod genes, was introduced into Agrobacterium tumefaciens. The resulting transconjugants induced root deformations specifically on the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.157.1.134-142.1984

    authors: Truchet G,Rosenberg C,Vasse J,Julliot JS,Camut S,Denarie J

    更新日期:1984-01-01 00:00:00

  • Sucrose-dependent spectinomycin-resistant mutants of Escherichia coli.

    abstract::Spectinomycin-resistant (Spcr) mutants of Escherichia coli were isolated from nutrient agar plates containing 20% sucrose and 100 mug of spectinomycin per ml. About one-third of the Spcr mutants thus obtained were sucrose dependent (Sucd) and were classified into two types: I, those unable to grow on sucrose-free medi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.125.1.142-148.1976

    authors: Miyoshi Y,Yamagata H

    更新日期:1976-01-01 00:00:00

  • Adherence of the gram-positive bacterium Ruminococcus albus to cellulose and identification of a novel form of cellulose-binding protein which belongs to the Pil family of proteins.

    abstract::The adherence of Ruminococcus albus 8 to crystalline cellulose was studied, and an affinity-based assay was also used to identify candidate cellulose-binding protein(s). Bacterial adherence in cellulose-binding assays was significantly increased by the inclusion of either ruminal fluid or micromolar concentrations of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.22.5921-5927.1998

    authors: Pegden RS,Larson MA,Grant RJ,Morrison M

    更新日期:1998-11-01 00:00:00

  • Enzyme distribution in Pseudomonas aeruginosa.

    abstract::Campbell, J. J. R. (The University of British Columbia, Vancouver, B.C., Canada), Loretta A. Hogg, and G. A. Strasdine. Enzyme distribution in Pseudomonas aeruginosa. J. Bacteriol. 83:1155-1160. 1962.-Previous studies on the distribution of enzymes in bacteria have indicated that, although individual enzymes were pred...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.5.1155-1160.1962

    authors: CAMPBELL JJ,HOGGLA,STRASDINE GA

    更新日期:1962-05-01 00:00:00

  • Regulation of breakdown and synthesis of L-glutamate decarboxylase in Clostridium perfringens.

    abstract::L-Glutamate decarboxylase (GAD) activity of Clostridium perfringens (ATCC 8009) cells grown in various culture conditions was investigated. Remarkable variations of GAD level occur during the growth cycle in thioglycollate broth. These changes are affected by the pH of the culture medium. Addition of alkali to the cul...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.123.3.1115-1123.1975

    authors: Cozzani I,Barsacchi R,Dibenedetto G,Saracchi L,Falcone G

    更新日期:1975-09-01 00:00:00

  • Galactose transport in Saccharomyces cerevisiae. I. Nonmetabolized sugars as substrates and inducers of the galactose transport system.

    abstract::The inducible galactose transport system in bakers' yeast carries out the facilitated diffusion of the nonmetabolized galactose analogues d-fucose and l-arabinose. This capacity depends on the activity of the Ga 2 gene. In some strains, d-fucose and l-arabinose are also gratuitous inducers. Mutants in which the induci...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.5.1727-1731.1968

    authors: Cirillo VP

    更新日期:1968-05-01 00:00:00

  • Overlapping mRNA transcripts of photosynthesis gene operons in Rhodobacter capsulatus.

    abstract::The crtEF, bchCA, and puf operons of the facultative phototrophic bacterium Rhodobacter capsulatus encode gene products that are necessary for the formation of various components of the photosynthetic apparatus. The crtEF operon encodes two enzymes involved in the biosynthesis of carotenoids, the bchCA operon codes fo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.4.1432-1443.1991

    authors: Wellington CL,Beatty JT

    更新日期:1991-02-01 00:00:00

  • MxiJ, a lipoprotein involved in secretion of Shigella Ipa invasins, is homologous to YscJ, a secretion factor of the Yersinia Yop proteins.

    abstract::Shigella flexneri causes bacillary dysentery by invading epithelial cells of the colonic mucosa. The invasion process requires the synthesis and secretion of the virulence plasmid-encoded Ipa proteins. Using TnphoA mutagenesis, we have identified two virulence plasmid genes, mxiJ and mxiM, that encode proteins exporte...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.23.7661-7669.1992

    authors: Allaoui A,Sansonetti PJ,Parsot C

    更新日期:1992-12-01 00:00:00

  • Probing the structure, function, and interactions of the Escherichia coli H-NS and StpA proteins by using dominant negative derivatives.

    abstract::Twelve different dominant negative mutants of the Escherichia coli nucleoid-associated protein, H-NS, have been selected and characterized in vivo. The mutants are all severely defective in promoter repression activity in a strain lacking H-NS, and they all disrupt the repression normally exerted by H-NS at two of its...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.15.4335-4343.1996

    authors: Williams RM,Rimsky S,Buc H

    更新日期:1996-08-01 00:00:00

  • Molecular cloning of TOL genes xylB and xylE in Escherichia coli.

    abstract::The xylB and xylE genes in the TOL plasmid of Pseudomonas putida mt-2, which code for benzyl alcohol dehydrogenase and catechol 2,3-oxygenase, respectively, were cloned onto plasmid pBR322 in Escherichia coli for detailed mapping. The xylB gene was mapped in a 2.9-kilobase region within the BamHI BC fragment of pTN2, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.145.3.1137-1143.1981

    authors: Inouye S,Nakazawa A,Nakazawa T

    更新日期:1981-03-01 00:00:00

  • Detection of an A-factor-responsive protein that binds to the upstream activation sequence of strR, a regulatory gene for streptomycin biosynthesis in Streptomyces griseus.

    abstract::DNA-binding assays using mobility shift polyacrylamide gel electrophoresis revealed the presence of a protein that specifically bound to a restriction fragment -288 to -191 bp upstream from the transcriptional start point of strR, a regulatory gene for streptomycin biosynthesis in Streptomyces griseus. The binding sit...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.9.2652-2661.1993

    authors: Vujaklija D,Horinouchi S,Beppu T

    更新日期:1993-05-01 00:00:00

  • Dependence of ribonucleic acid synthesis on continuous protein synthesis in yeast.

    abstract::Using an auxotrophic strain of Saccharomyces cerevisiae, we examined the kinetics of ribonucleic acid (RNA) synthesis following inhibition of protein synthesis caused by amino acid starvation or cycloheximide. Removal of a required amino acid immediately stopped net protein synthesis. After a brief lag, RNA synthesis ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.109.2.773-779.1972

    authors: Roth RM,Dampier C

    更新日期:1972-02-01 00:00:00

  • NOD2/c-Jun NH2-Terminal Kinase Triggers Mycoplasma ovipneumoniae-Induced Macrophage Autophagy.

    abstract::Mycoplasma ovipneumoniae belongs to Mycoplasma, a genus containing the smallest self-replicating microorganisms, and causes infectious pleuropneumonia in goats and sheep. Nucleotide-binding oligomerization domain-containing protein (NOD2), an intracellular pattern recognition receptor, interacts with muramyl dipeptide...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00689-19

    authors: Luo H,Wu X,Xu Z,Hao X,Wang Y,Li M

    更新日期:2020-09-23 00:00:00

  • Genome sequence of Lactococcus garvieae 8831, isolated from rainbow trout lactococcosis outbreaks in Spain.

    abstract::Lactococcus garvieae is the etiological agent of lactococcosis, one of the most important disease threats to the sustainability of the rainbow trout farming industry. Here, we present the draft genome sequence of Lactococcus garvieae strain 8831, isolated from diseased rainbow trout, which is composed of 2,087,276 bp ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05326-11

    authors: Aguado-Urda M,López-Campos GH,Gibello A,Cutuli MT,López-Alonso V,Fernández-Garayzábal JF,Blanco MM

    更新日期:2011-08-01 00:00:00

  • Functions of the duplicated hik31 operons in central metabolism and responses to light, dark, and carbon sources in Synechocystis sp. strain PCC 6803.

    abstract::There are two closely related hik31 operons involved in signal transduction on the chromosome and the pSYSX plasmid in the cyanobacterium Synechocystis sp. strain PCC 6803. We studied the growth, cell morphology, and gene expression in operon and hik mutants for both copies, under different growth conditions, to exami...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06207-11

    authors: Nagarajan S,Sherman DM,Shaw I,Sherman LA

    更新日期:2012-01-01 00:00:00

  • Purification and characterization of a molybdenum-pterin-binding protein (Mop) in Clostridium pasteurianum W5.

    abstract::A large-scale fractionation scheme purified the major molybdenum(Mo)-binding protein (Mop) from crude extracts of Clostridium pasteurianum, with a 10 and 0.2% yield of Mo and protein, respectively. The apparent molecular weight of the purified molybdoprotein is 5,700, as determined by sodium dodecyl sulfate-polyacryla...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.168.2.688-693.1986

    authors: Hinton SM,Merritt B

    更新日期:1986-11-01 00:00:00

  • A slow-motility phenotype caused by substitutions at residue Asp31 in the PomA channel component of a sodium-driven flagellar motor.

    abstract::PomA is thought to be a component of the ion channel in the sodium-driven polar-flagellar motor of Vibrio alginolyticus. We have found that some cysteine substitutions in the periplasmic region of PomA result in a slow-motility phenotype, in which swarming and swimming speeds are reduced even in the presence of high c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.11.3314-3318.2000

    authors: Kojima S,Shoji T,Asai Y,Kawagishi I,Homma M

    更新日期:2000-06-01 00:00:00

  • Localization of quantitation of the ornithine lipid of Thiobacillus thiooxidans.

    abstract::The ornithine lipid of Thiobacillus thiooxidans was found to be 1.9% of the total polar lipids. Approximately 80% of this lipid was found to be localized in the outer membrane. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.149.2.798-799.1982

    authors: Dees C,Shively JM

    更新日期:1982-02-01 00:00:00

  • Isolation of a mutant of Escherichia coli defective in cytosine-specific deoxyribonucleic acid methylase activity and in partial protection of bacteriophage lambda against restriction by cells containing the N-3 drug-resistance factor.

    abstract::A mutant (designated mec(-)) of Escherichia coli F(+) 100 endo I(-)su(+) r(K) (-)m(K) (+) has been isolated which is defective in cytosine-specific deoxyribonucleic acid (DNA) methylase activity. The DNA of this mutant, as well as the DNA of phages lambda and fd propagated in it, is virtually devoid of 5-methyl-cytosi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.115.3.1103-1107.1973

    authors: Hattman S,Schlagman S,Cousens L

    更新日期:1973-09-01 00:00:00

  • IS257-mediated cointegration in the evolution of a family of staphylococcal trimethoprim resistance plasmids.

    abstract::Analyses of the Staphylococcus epidermidis multiresistance plasmids pSK697 and pSK818 have revealed them to be closely related to the trimethoprim resistance plasmid pSK639, also isolated from S. epidermidis. pSK697 and pSK818 were found to contain a cointegrated copy of a second plasmid related to the S. epidermidis ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.20.6070-6073.1996

    authors: Leelaporn A,Firth N,Paulsen IT,Skurray RA

    更新日期:1996-10-01 00:00:00

  • AbrB modulates expression and catabolite repression of a Bacillus subtilis ribose transport operon.

    abstract::A Bacillus subtilis ribose transport operon (rbs) was shown to be subject to AbrB-mediated control through direct AbrB-DNA binding interactions in the vicinity of the promoter. Overproduction of AbrB was shown to relieve catabolite repression of rbs during growth in the presence of poorer carbon sources such as arabin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.23.6727-6731.1995

    authors: Strauch MA

    更新日期:1995-12-01 00:00:00

  • EbfC (YbaB) is a new type of bacterial nucleoid-associated protein and a global regulator of gene expression in the Lyme disease spirochete.

    abstract::Nearly every known species of Eubacteria encodes a homolog of the Borrelia burgdorferi EbfC DNA-binding protein. We now demonstrate that fluorescently tagged EbfC associates with B. burgdorferi nucleoids in vivo and that chromatin immunoprecipitation (ChIP) of wild-type EbfC showed it to bind in vivo to sites througho...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00252-12

    authors: Jutras BL,Bowman A,Brissette CA,Adams CA,Verma A,Chenail AM,Stevenson B

    更新日期:2012-07-01 00:00:00

  • Localization of acyl carrier protein in Escherichia coli.

    abstract::Acyl carrier protein was localized by immunoelectron microscopy in the cytoplasm of Escherichia coli. These data are inconsistent with the previous report of an association between acyl carrier protein and the inner membrane (H. Van den Bosch, J.R. Williamson, and P.R. Vagelos, Nature [London] 228:338-341, 1970). More...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.162.1.5-8.1985

    authors: Jackowski S,Edwards HH,Davis D,Rock CO

    更新日期:1985-04-01 00:00:00

  • Characterization of Brucella suis clpB and clpAB mutants and participation of the genes in stress responses.

    abstract::Pathogens often encounter stressful conditions inside their hosts. In the attempt to characterize the stress response in Brucella suis, a gene highly homologous to Escherichia coli clpB was isolated from Brucella suis, and the deduced amino acid sequence showed features typical of the ClpB ATPase family of stress resp...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.8.2677-2681.2001

    authors: Ekaza E,Teyssier J,Ouahrani-Bettache S,Liautard JP,Köhler S

    更新日期:2001-04-01 00:00:00

  • Contribution of the periplasmic chaperone Skp to efficient presentation of the autotransporter IcsA on the surface of Shigella flexneri.

    abstract::IcsA is an outer membrane protein in the autotransporter family that is required for Shigella flexneri pathogenesis. Following its secretion through the Sec translocon, IcsA is incorporated into the outer membrane in a process that depends on YaeT, a component of an outer membrane beta-barrel insertion machinery. We i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00989-08

    authors: Wagner JK,Heindl JE,Gray AN,Jain S,Goldberg MB

    更新日期:2009-02-01 00:00:00

  • Identification of phosphate starvation-inducible genes in Escherichia coli K-12 by DNA sequence analysis of psi::lacZ(Mu d1) transcriptional fusions.

    abstract::Twenty-four independent phosphate starvation-inducible (psi) transcriptional fusions made with Mu d1(lacZbla) were analyzed by sequencing the psi::lacZ(Mu d1) chromosomal junctions by using DNAs amplified with the polymerase chain reaction or mini-Mu cloning. Our DNA sequence analysis showed that the MuR DNA in Mu d1 ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.6.3191-3200.1990

    authors: Metcalf WW,Steed PM,Wanner BL

    更新日期:1990-06-01 00:00:00

  • Plant signal molecules activate the syrB gene, which is required for syringomycin production by Pseudomonas syringae pv. syringae.

    abstract::The syrB gene is required for syringomycin production by Pseudomonas syringae pv. syringae and full virulence during plant pathogenesis. Strain B3AR132 containing a syrB::lacZ fusion was used to detect transcriptional activation of the syrB gene in syringomycin minimal medium by plant metabolites with signal activity....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.18.5784-5792.1991

    authors: Mo YY,Gross DC

    更新日期:1991-09-01 00:00:00

  • Genes controlling xylan utilization by Bacillus subtilis.

    abstract::Eight mutants of Bacillus subtilis deficient in xylan utilization were isolated and characterized genetically and biochemically. Each mutant was obtained independently after nitrosoguanidine mutagenesis. All of the analyzed mutations were shown to be linked. Reciprocal transformation crosses revealed the existence of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.156.1.257-263.1983

    authors: Roncero MI

    更新日期:1983-10-01 00:00:00

  • Rhizobium meliloti swims by unidirectional, intermittent rotation of right-handed flagellar helices.

    abstract::The 5 to 10 peritrichously inserted complex flagella of Rhizobium meliloti MVII-1 were found to form right-handed flagellar bundles. Bacteria swam at speeds up to 60 microns/s, their random three-dimensional walk consisting of straight runs and quick directional changes (turns) without the vigorous angular motion (tum...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.7.3146-3150.1987

    authors: Götz R,Schmitt R

    更新日期:1987-07-01 00:00:00

  • Linear- and circular-plasmid copy numbers in Borrelia burgdorferi.

    abstract::Borrelia burgdorferi, the Lyme disease agent, and other members of the spirochetal genus Borrelia have double-stranded linear plasmids in addition to supercoiled circular plasmids. The copy number relative to the chromosome was determined for 49- and 16-kb linear plasmids and a 27-kb circular plasmid of the type strai...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.16.5251-5257.1992

    authors: Hinnebusch J,Barbour AG

    更新日期:1992-08-01 00:00:00