Mutational analysis of cat-86 gene expression controlled by lactococcal promoters in Lactococcus lactis subsp. lactis and Escherichia coli.

abstract：:The acetogenic bacterium Clostridium thermoautotrophicum, grown on methanol, glucose, or CO2-H2, contained high levels of corrinoids, formate dehydrogenase, tetrahydrofolate enzymes, carbon monoxide dehydrogenase, and hydrogenase. Cell-free extracts catalyzed pyruvate-dependent formation of acetate from methyltetrahyd...

journal_title：Journal of bacteriology

authors： Clark JE,Ragsdale SW,Ljungdahl LG,Wiegel J

更新日期：1982-07-01 00:00:00

abstract：:The metabolism of one- and two-carbon compounds by the methylotrophic bacterium Methylobacterium extorquens AM1 involves high carbon flux through the ethylmalonyl coenzyme A (ethylmalonyl-CoA) pathway (EMC pathway). During growth on ethylamine, the EMC pathway operates as a linear pathway carrying the full assimilator...

journal_title：Journal of bacteriology

authors： Good NM,Martinez-Gomez NC,Beck DA,Lidstrom ME

更新日期：2015-02-15 00:00:00

abstract：:The Rickettsia prowazekii sigma factor was overexpressed, purified, and used to reconstitute RNA polymerase holoenzyme species. R. prowazekii RNA polymerase-promoter complexes were unstable and remained dissociable and heparin sensitive under conditions in which the corresponding Escherichia coli complexes were not. T...

journal_title：Journal of bacteriology

authors： Aniskovitch LP,Winkler HH

更新日期：1995-11-01 00:00:00

abstract：:Comamonas testosteroni T-2 uses a standard, if seldom examined, attack on an aromatic compound and oxygenates the side chain of p-toluenesulfonate (TS) (or p-toluenecarboxylate) to p-sulfobenzoate (or terephthalate) prior to complete oxidation. The expression of the first three catabolic enzymes in the pathway, the TS...

journal_title：Journal of bacteriology

authors： Junker F,Kiewitz R,Cook AM

更新日期：1997-02-01 00:00:00

abstract：:Aspartate transcarbamylase is synthesized during exponential growth of Bacillus subtilis and is inactivated when the cells enter the stationary phase. This work is a study of the regulation of aspartate transcarbamylase synthesis during growth and the stationary phase. Using specific immunoprecipitation of aspartate t...

journal_title：Journal of bacteriology

authors： Maurizi MR,Switzer RL

更新日期：1978-09-01 00:00:00

abstract：:Mutations in two chromosomal genes of Escherichia coli, cpxA and cpxB, produced a temperature-sensitive growth defect that was remedied specifically by the addition of isoleucine and valine to the minimal medium. This auxotrophy was manifested only when the medium contained exogenous leucine, suggesting that mutant ce...

journal_title：Journal of bacteriology

authors： McEwen J,Silverman P

更新日期：1980-10-01 00:00:00

abstract：:We isolated an unusual mutant in which the arabinose operon carried an araC lesion that led to a dominant fucose resistance while retaining recessive constitutivity. All previously characterized C(c) mutations have been recessive for both fucose resistance and constitutivity. ...

journal_title：Journal of bacteriology

authors： Nathanson NM,Schleif R

更新日期：1973-08-01 00:00:00

abstract：:The Escherichia coli flaA gene product (also called cheC) plays a crucial role in switching flagellar rotational direction during chemotactic responses. Wild-type and mutant alleles have been cloned onto plasmid vectors, and the gene product has been identified as a 37,000-dalton protein. The flaA product appeared as ...

journal_title：Journal of bacteriology

authors： Clegg DO,Koshland DE Jr

更新日期：1985-04-01 00:00:00

abstract：:The effects of competence-inducing treatments on the composition and organization of membrane lipids in Escherichia coli K-12, DH1, DH5, HB101, and RR1 were investigated for two widely used protocols in which transformability is developed at low temperatures in Ca2+ buffers. At stages during each procedure, the lipid ...

journal_title：Journal of bacteriology

authors： Reusch RN,Hiske TW,Sadoff HL

更新日期：1986-11-01 00:00:00

abstract：:At various stages during spore formation sporangia were shocked by cold treatment or with toluene, and the germination requirements of the prespores were examined. Up to 5 h after induction of sporulation (t5) germination was spontaneous; i.e., it occurred without any added germinants. After t5, during stages V and VI...

journal_title：Journal of bacteriology

authors： Dion P,Mandelstam J

更新日期：1980-02-01 00:00:00

abstract：:Sortases are transamidases that covalently link proteins to the peptidoglycan of gram-positive bacteria. The genome of the pathogenic bacterium Listeria monocytogenes encodes two sortases genes, srtA and srtB. The srtA gene product anchors internalin and some other LPXTG-containing proteins to the listerial surface. H...

journal_title：Journal of bacteriology

authors： Bierne H,Garandeau C,Pucciarelli MG,Sabet C,Newton S,Garcia-del Portillo F,Cossart P,Charbit A

更新日期：2004-04-01 00:00:00

abstract：:Members of the pPT23A plasmid family of Pseudomonas syringae play an important role in the interaction of this bacterial pathogen with host plants. Complete sequence analysis of several pPT23A family plasmids (PFPs) has provided a glimpse of the gene content and virulence function of these plasmids. We constructed a m...

journal_title：Journal of bacteriology

authors： Zhao Y,Ma Z,Sundin GW

更新日期：2005-03-01 00:00:00

abstract：:Yeast mutants deficient in orotidine-5'-phosphate (OMP) pyrophosphorylase activity have been obtained. The gene encoding this enzyme is unlinked to the four pyrimidine genes previously described. The specific activity of OMP pyrophosphorylase remains constant under all of the physiological conditions used to repress, ...

journal_title：Journal of bacteriology

authors： Jund R,Lacroute F

更新日期：1972-01-01 00:00:00

abstract：:Replication initiation depends on origin recognition, helicase, and primase activities. In phage P4, a second DNA region, the cis replication region (crr), is also required for replication initiation. The multifunctional alpha protein of phage P4, which is essential for DNA replication, combines the three aforemention...

journal_title：Journal of bacteriology

authors： Ziegelin G,Calendar R,Lurz R,Lanka E

更新日期：1997-07-01 00:00:00

abstract：:The conjugally acquired deoxyribonucleic acid (DNA) of small, anucleate cells ("minicells") of a mutant strain of Escherichia coli K-12 was found to be predominantly associated with the bacterial membrane. Evidence from X-irradiation studies in vivo shows that there is no decrease in DNA-membrane association under con...

journal_title：Journal of bacteriology

authors： Shull FW Jr,Fralick JA,Stratton LP,Fisher WD

更新日期：1971-05-01 00:00:00

abstract：:The R1162-encoded protein RepIB is essential for replication of the plasmid and binds specifically to iterons within the replicative origin. The protein causes the localized melting of DNA (determined by sensitivity to P1 nuclease) at a site within the AT-rich region of the origin, about 60 bp from the iteron binding ...

journal_title：Journal of bacteriology

authors： Kim YJ,Meyer RJ

更新日期：1991-09-01 00:00:00

abstract：:An endonuclease was detected in strains of Salmonella typhimurium containing the drug resistance plasmid pKM101. The enzyme was not detectable in strains lacking this plasmid, but it was present in strains containing mutants of pKM101 that were no longer able to enhance host cell mutagenesis. The endonuclease had a mo...

journal_title：Journal of bacteriology

authors： Lackey D,Walker GC,Keng T,Linn S

更新日期：1977-08-01 00:00:00

abstract：:A 4.2-kb plasmid (pLV22a) native to Bacteroides fragilis LV22 became fused to a transfer-deficient Bacteroides spp.-Escherichia coli shuttle vector by an inverse transposition event, resulting in a transferrable phenotype. The transfer phenotype was attributable to pLV22a, which was also capable of mobilization within...

journal_title：Journal of bacteriology

authors： Novicki TJ,Hecht DW

更新日期：1995-08-01 00:00:00

abstract：:Beggs, William H. (University of Minnesota, Minneapolis), and Palmer Rogers. Galactose repression of beta-galactosidase induction in Escherichia coli. J. Bacteriol. 91:1869-1874. 1966.-Galactose repression of beta-galactosidase induction in Escherichia coli was investigated to determine whether the galactose molecule ...

journal_title：Journal of bacteriology

authors： Beggs WH,Rogers P

更新日期：1966-05-01 00:00:00

abstract：:Methionine auxotrophs of Saccharomyces cerevisiae continue to synthesize ribonucleic acid (RNA) after methionine withdrawal. The newly synthesized transfer RNA (tRNA) is methyl-deficient in some strains, but not in all. Whether such tRNA will accumulate depends on the position of the block in the methionine pathway th...

journal_title：Journal of bacteriology

authors： Kjellin-Stråby K

更新日期：1969-11-01 00:00:00

abstract：:For the pathogenic yeast Candida albicans, secreted aspartyl proteinase (Sap) activity has been correlated with virulence. A family consisting of at least eight SAP genes can be drawn upon to produce Sap enzymatic activity. In this study, the levels of Sap1, Sap2, and Sap3 isoenzymes were monitored under a variety of ...

journal_title：Journal of bacteriology

authors： White TC,Agabian N

更新日期：1995-09-01 00:00:00

abstract：:The endophytic diazotroph Azoarcus sp. strain BH72 is capable of infecting rice roots and of expressing the nitrogenase (nif) genes there. In order to study the genetic background for nitrogen fixation in strain BH72, the structural genes of nitrogenase (nifHDK) were cloned and sequenced. The sequence analysis reveale...

journal_title：Journal of bacteriology

authors： Egener T,Martin DE,Sarkar A,Reinhold-Hurek B

更新日期：2001-06-01 00:00:00

abstract：:Two temperature-sensitive sporulation mutants have been characterized. One mutant, which is blocked at stage II, has a short temperature-sensitive period that occurs at about the time when the spore septum is formed. Cells can escape the sporulation block, if incubated for a short period at the permissive temperature,...

journal_title：Journal of bacteriology

authors： Young M

更新日期：1976-05-01 00:00:00

abstract：:For maximal turbidity and dissimilatory activity, three strains of pediococci required the addition of Mn(2+) to Rogosa basal medium. Twenty-two strains of enterococci proved indifferent to this supplement. ...

journal_title：Journal of bacteriology

authors： Efthymiou CJ,Joseph SW

更新日期：1972-10-01 00:00:00

abstract：:The Rhizobium phaseoli recA gene has been cloned by interspecific complementation of the Fec phenotype of bacteriophage lambda. The cloned gene restored the recombination proficiency and conferred resistance to DNA-damaging agents (methyl methanesulfonate and nitrofurantoin) to an Escherichia coli recA mutant. The dir...

journal_title：Journal of bacteriology

authors： Martínez-Salazar JM,Romero D,Girard ML,Dávila G

更新日期：1991-05-01 00:00:00

abstract：:The gram-positive, thermophilic, acetogenic bacterium Moorella thermoacetica can reduce CO2 to acetate via the Wood-Ljungdahl (acetyl coenzyme A synthesis) pathway. This report demonstrates that, despite its classification as a strict anaerobe, M. thermoacetica contains a membrane-bound cytochrome bd oxidase that can ...

journal_title：Journal of bacteriology

authors： Das A,Silaghi-Dumitrescu R,Ljungdahl LG,Kurtz DM Jr

更新日期：2005-03-01 00:00:00

abstract：:Martinez, R. J. (University of California, Los Angeles), and E. Z. Gordee. Formation of bacterial flagella. I. Demonstration of a functional flagellin pool in Spirillum serpens and Bacillus subtilis. J. Bacteriol. 91:870-875. 1966-Exponentially growing cultures of Spirillum serpens and Bacillus subtilis regained motil...

journal_title：Journal of bacteriology

authors： Martinez RJ,Gordee EZ

更新日期：1966-02-01 00:00:00

abstract：:Motility in mycobacteria was described for the first time in 1999. It was reported that Mycobacterium smegmatis and Mycobacterium avium could spread on the surface of solid growth medium by a sliding mechanism and that the presence of cell wall glycopeptidolipids was essential for motility. We recently reported that M...

journal_title：Journal of bacteriology

authors： Agustí G,Astola O,Rodríguez-Güell E,Julián E,Luquin M

更新日期：2008-10-01 00:00:00

abstract：:The genes encoding Shiga toxin (stx), the major virulence factor of Shiga toxin-encoding Escherichia coli (STEC) strains, are carried on lambdoid prophages resident in all known STEC strains. The stx genes are expressed only during lytic growth of these temperate bacteriophages. We cloned the gene encoding the repress...

journal_title：Journal of bacteriology

authors： Koudelka AP,Hufnagel LA,Koudelka GB

更新日期：2004-11-01 00:00:00

abstract：:The Salmonella typhimurium LT2 ilvYC genes were studied by fusion of each gene to the Escherichia coli K-12 galK gene. The expression of ilvY and ilvC could then be determined by measurement of the galK-encoded galactokinase enzyme. The promoter for ilvC, pC, was located by this technique to a 0.42-kilobase BglII-EcoR...

journal_title：Journal of bacteriology

authors： Blazey DL,Burns RO

更新日期：1984-09-01 00:00:00