Abstract:
:At various stages during spore formation sporangia were shocked by cold treatment or with toluene, and the germination requirements of the prespores were examined. Up to 5 h after induction of sporulation (t5) germination was spontaneous; i.e., it occurred without any added germinants. After t5, during stages V and VI, the capacity for spontaneous germination diminished progressively, and the spores acquired a need for externally added germinants. At t6 this need was satisfied by either L-alanine or a mixture of KCl, glucose, and fructose. By t8, the latter response had disappeared. The spores germinated only with L-alanine, and the response was much slower. Experiments with chloramphenicol showed that the germination properties of the spores appearing between t6 and t8 were the expression of events in protein synthesis that had occurred before t5. Although the germination requirements developed at about the same time as heat resistance, they could be dissociated from heat resistance in wild-type and mutant cells. The germination properties of the developing spores are additional marker events characterizing the later stages of sporulation, as follows: (i) spontaneous germination (up to the end of stage IV); (ii) germination requirements that are satisfied by KCl-glucose-fructose or L-alanine (stage V); and (iii) slow germination response with L-alanine only (stage VI).
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Dion P,Mandelstam Jdoi
10.1128/JB.141.2.786-792.1980subject
Has Abstractpub_date
1980-02-01 00:00:00pages
786-92issue
2eissn
0021-9193issn
1098-5530journal_volume
141pub_type
杂志文章abstract::The crystal structure of the Na(+)-coupled melibiose permease of Salmonella enterica serovar Typhimurium (MelBSt) demonstrates that MelB is a member of the major facilitator superfamily of transporters. Arg residues at positions 295, 141, and 363 are involved in interdomain interactions at the cytoplasmic side by gove...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01868-14
更新日期:2014-09-01 00:00:00
abstract::The abundance of oxidants and reductants must be balanced for an organism to thrive. Bacteria have evolved methods to prevent redox imbalances and to mitigate their deleterious consequences through the expression of detoxification enzymes, antioxidants, and systems to repair or degrade damaged proteins and DNA. Regula...
journal_title:Journal of bacteriology
pub_type: 杂志文章,评审
doi:10.1128/JB.00128-18
更新日期:2018-08-10 00:00:00
abstract::We have evaluated the use of [1,2-13C2]propionate for the analysis of propionic acid metabolism, based on the ability to distinguish between the methylcitrate and methylmalonate pathways. Studies using propionate-adapted Escherichia coli MG1655 cells were performed. Preservation of the 13C-13C-12C carbon skeleton in l...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.11.3562-3570.1999
更新日期:1999-06-01 00:00:00
abstract::Site-directed mutagenesis has been used to identify a number of charged residues essential for the transport activity of the PheP protein. These residues are highly conserved in the cluster of amino acid transporters. However, some other conserved residues and a number of aromatic residues have been shown not to be es...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.22.7500-7504.1993
更新日期:1993-11-01 00:00:00
abstract::Listeria monocytogenes has caused numerous human outbreaks. Here we report draft genomes of L. monocytogenes J1816 and J1-220, which belong to epidemic clones II and IV, respectively. Whole-genome sequence analysis of these strains provides a tool for studying the short-term evolution of these epidemic clones. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05048-11
更新日期:2011-07-01 00:00:00
abstract::Penicillin-binding protein 2 (PBP-2) of Escherichia coli K-12 was purified by covalent affinity chromatography using 6-aminopenicillanic acid covalently coupled to carboxymethyl-Sepharose (6-APA-CM-Sepharose). Purification of PBP-2 was accomplished by prebinding the methoxy cephalosporin, cefoxitin, to the Triton X-10...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.145.1.398-403.1981
更新日期:1981-01-01 00:00:00
abstract:UNLABELLED:Edwardsiella tarda is an important pathogenic bacterium that can replicate in macrophages. However, how the intramacrophage infection process affects the virulence of this bacterium is essentially unknown. Here, we show that E. tarda replicates and induces a caspase-1-dependent cell pyroptosis in a murine ma...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00978-15
更新日期:2016-04-28 00:00:00
abstract::A gene coding for thermophilic beta-amylase of Clostridium thermosulfurogenes was cloned into Bacillus subtilis, and its nucleotide sequence was determined. The nucleotide sequence suggested that the thermophilic beta-amylase is translated from monocistronic mRNA as a secretory precursor with a signal peptide of 32 am...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.12.5848-5854.1988
更新日期:1988-12-01 00:00:00
abstract::The Dps-like peroxide resistance protein (Dpr) is essential for H2O2 stress tolerance and aerobic growth of the oral pathogen Streptococcus mutans Dpr accumulates during oxidative stress, protecting the cell by sequestering iron ions and thereby preventing the generation of toxic hydroxyl radicals that result from the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00062-18
更新日期:2018-06-25 00:00:00
abstract::In this study, we demonstrated the remarkable genome plasticity of lytic lactococcal phages that allows them to rapidly adapt to the dynamic dairy environment. The lytic double-stranded DNA phage ul36 was used to sequentially infect a wild-type strain of Lactococcus lactis and two isogenic derivatives with genes encod...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01111-06
更新日期:2007-02-01 00:00:00
abstract::The nucleotide sequence of the agp gene, which encodes a periplasmic glucose-1-phosphatase, was determined. The deduced amino acid sequence corresponds to a 413-amino-acid-residue polypeptide with a typical hydrophobic signal sequence of 22 amino acids. The mature protein lacks the N-terminal signal peptide and has a ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.2.802-807.1990
更新日期:1990-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.8.2368-2374.1996
更新日期:1996-04-01 00:00:00
abstract::Extracellular fibrils are involved in cell cohesion and cell development in Myxococcus xanthus. One group of social motility mutants, Dsp, is unable to produce extracellular fibrils; these mutants also lose the abilities to cohere and to develop. Extracellular fibrils isolated from vegetative wild-type cells and added...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.23.7190-7196.1994
更新日期:1994-12-01 00:00:00
abstract::MntH, a bacterial homolog of mammalian natural resistance associated macrophage protein 1 (Nramp1), is a primary transporter for Mn(2+) influx in Salmonella enterica serovar Typhimurium and Escherichia coli. S. enterica serovar Typhimurium MntH contributes to H(2)O(2) resistance and is important for full virulence. Co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.12.3151-3158.2002
更新日期:2002-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.16.5286-5288.1993
更新日期:1993-08-01 00:00:00
abstract::The method of shuttle mutagenesis has been extended to Neisseria gonorrhoeae. We have constructed a defective mini-Tn3 derivative that encodes chloramphenicol resistance in both N. gonorrhoeae and Escherichia coli and selected for mutations in the chloramphenicol resistance gene that express higher levels of antibioti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.1.40-46.1990
更新日期:1990-01-01 00:00:00
abstract::Expression of a circadian rhythm of conidiation in Neurospora crassa is enhanced in cultures grown on medium that is neutral or deficient in biotin. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.123.1.387-389.1975
更新日期:1975-07-01 00:00:00
abstract::The presence of the membrane lipid phosphatidylcholine (PC) in the bacterial membrane is critically important for many host-microbe interactions. The phospholipid N-methyltransferase PmtA from the plant pathogen Agrobacterium tumefaciens catalyzes the formation of PC by a three-step methylation of phosphatidylethanola...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01539-10
更新日期:2011-07-01 00:00:00
abstract::Herbaspirillum sp. strain GW103 was isolated from rhizosphere soil of the reed Phragmites australis on reclaimed land. Here we report the 5.05-Mb draft genome sequence of the strain, providing bioinformation about the agronomic benefits of this strain, such as multiple traits relevant to plant root colonization and pl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00806-12
更新日期:2012-08-01 00:00:00
abstract::Internal and surface structures of asci and ascospores were studied by transmission electron microscopy (TEM) and by scanning electron microscopy (SEM) to establish the character and number of ascospores within the ascus of Metschnikowia krissii. Enzyme digestion with snail gut enzymes and SEM examination suggested th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.115.1.316-322.1973
更新日期:1973-07-01 00:00:00
abstract::A phospholipase hydrolyzing cardiolipin to phosphatidic acid and phosphatidyl glycerol was characterized in gram-negative bacteria but was absent in preparations of gram-positive bacteria, Saccharomyces cerevisiae, and rat liver mitochondria. In cell-free extracts of Escherichia coli, Salmonella typhimurium, Proteus v...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.3.1148-1152.1975
更新日期:1975-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.130.1.1-3.1977
更新日期:1977-04-01 00:00:00
abstract::An exonuclease III-deficient strain of Escherichia coli K-12, BW2001 (xthA11), was unable to perform rapid repair of X-ray-induced deoxyribonucleic acid single-strand breaks and appeared to have a defect in the priming of the 3'-termini necessary for initiation of repair synthesis at the breaks. This defect cannot be ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.141.3.1424-1427.1980
更新日期:1980-03-01 00:00:00
abstract::ParB is one of two P1-encoded proteins that are required for active partition of the P1 prophage in Escherichia coli. To probe the native domain structure of ParB, we performed limited proteolytic digestions of full-length ParB, as well as of several N-terminal and C-terminal deletion fragments of ParB. The C-terminal...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.19.5898-5908.1999
更新日期:1999-10-01 00:00:00
abstract::Primer extension analysis of transcripts of the Bacillus subtilis autolysin (cwlB) operon indicated that SigD-dependent transcripts from the Pd promoter are missing in the degU32(Hy) and degS200 (Hy) mutants. The degU32(Hy) mutation caused a 99% reduction in the expression of a sigD-lacZ translational fusion gene cons...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.16.5177-5180.1994
更新日期:1994-08-01 00:00:00
abstract::Penicillium chrysogenum is an important producer of penicillin antibiotics. A key step in their biosynthesis is the oxidative cyclization of delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) to isopenicillin N by the enzyme isopenicillin N synthase (IPNS). bis-ACV, the oxidized disulfide form of ACV is, however, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.4.973-984.1994
更新日期:1994-02-01 00:00:00
abstract::The lipoteichoic acids were isolated from phenol extracts of four Listeria strains representing serotypes 4a, 4b, 6a, and 6 to compare the differences in structure of amphiphilic polysaccharides from various serotypes of Listeria spp. The lipoteichoic acids from the four strains examined had the same structure in both...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.168.1.115-122.1986
更新日期:1986-10-01 00:00:00
abstract::The fine structural changes associated with cell growth and toxin production have been examined in Vibrio cholerae strain 569B. No morphological alterations in the cell envelope are apparent during logarithmic growth with thin-section techniques. However, internal swelling, suggesting alteration of cell envelope perme...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.3.1393-1401.1969
更新日期:1969-12-01 00:00:00
abstract::The multicellular developmental cycle of Myxococcus xanthus requires large-scale changes in gene transcription, and recent findings indicate that NtrC-like activators play a prominent role in regulating these changes. In this study, we made insertions in 28 uncharacterized ntrC-like activator (nla) genes and found tha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.20.6083-6094.2003
更新日期:2003-10-01 00:00:00
abstract::The genetic requirements for the excision repair of thymine glycols, urea residues, and apurinic (AP) sites were examined by measuring the survival in Escherichia coli mutants of phi X174 replicative form (RF) I transfecting DNA containing selectively introduced lesions. phi X RF I DNA containing thymine glycols was i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.8.3359-3366.1988
更新日期:1988-08-01 00:00:00