Sortase B, a new class of sortase in Listeria monocytogenes.

Abstract:

:Sortases are transamidases that covalently link proteins to the peptidoglycan of gram-positive bacteria. The genome of the pathogenic bacterium Listeria monocytogenes encodes two sortases genes, srtA and srtB. The srtA gene product anchors internalin and some other LPXTG-containing proteins to the listerial surface. Here, we focus on the role of the second sortase, SrtB. Whereas SrtA acts on most of the proteins in the peptidoglycan fraction, SrtB appears to target minor amounts of surface polypeptides. We identified one of the SrtB-anchored proteins as the virulence factor SvpA, a surface-exposed protein which does not contain the LPXTG motif. Therefore, as in Staphylococcus aureus, the listerial SrtB represents a second class of sortase in L. monocytogenes, involved in the attachment of a subset of proteins to the cell wall, most likely by recognizing an NXZTN sorting motif. The DeltasrtB mutant strain does not have defects in bacterial entry, growth, or motility in tissue-cultured cells and does not show attenuated virulence in mice. SrtB-mediated anchoring could therefore be required to anchor surface proteins involved in the adaptation of this microorganism to different environmental conditions.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Bierne H,Garandeau C,Pucciarelli MG,Sabet C,Newton S,Garcia-del Portillo F,Cossart P,Charbit A

doi

10.1128/jb.186.7.1972-1982.2004

keywords:

subject

Has Abstract

pub_date

2004-04-01 00:00:00

pages

1972-82

issue

7

eissn

0021-9193

issn

1098-5530

journal_volume

186

pub_type

杂志文章
  • Control of EpsE, the phosphoglycosyltransferase initiating exopolysaccharide synthesis in Streptococcus thermophilus, by EpsD tyrosine kinase.

    abstract::We studied the roles of Streptococcus thermophilus phosphogalactosyltransferase (EpsE) (the priming enzyme), tyrosine kinase (EpsD), phosphatase (EpsB), and a membrane-associated protein with no known biochemical function (EpsC) in exopolysaccharide (EPS) synthesis. These proteins are well-conserved among bacteria and...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01122-06

    authors: Minic Z,Marie C,Delorme C,Faurie JM,Mercier G,Ehrlich D,Renault P

    更新日期:2007-02-01 00:00:00

  • T-POP array identifies EcnR and PefI-SrgD as novel regulators of flagellar gene expression.

    abstract::The T-POP transposon was employed in a general screen for tetracycline (Tet)-induced chromosomal loci that exhibited Tet-activated or Tet-repressed expression of a fliC-lac transcriptional fusion. Insertions that activated flagellar transcription were located in flagellar genes. T-POP insertions that exhibited Tet-dep...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01177-08

    authors: Wozniak CE,Lee C,Hughes KT

    更新日期:2009-03-01 00:00:00

  • Isolation and characterization of Tn917lac-generated competence mutants of Bacillus subtilis.

    abstract::We isolated 28 mutants of Bacillus subtilis deficient in the development of competence by using the transposon Tn917lacZ as a mutagen. The mutant strains were poorly transformable with plasmid and chromosomal DNAs but were normally transducible and exhibited wild-type resistance to DNA-damaging agents. The mutations w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.7.3104-3109.1987

    authors: Hahn J,Albano M,Dubnau D

    更新日期:1987-07-01 00:00:00

  • Comparative structural and molecular characterization of ribitol-5-phosphate-containing Streptococcus oralis coaggregation receptor polysaccharides.

    abstract::The antigenically related coaggregation receptor polysaccharides (RPS) of Streptococcus oralis strains C104 and SK144 mediate recognition of these bacteria by other members of the dental plaque biofilm community. In the present study, the structure of strain SK144 RPS was established by high resolution NMR spectroscop...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01532-08

    authors: Yang J,Ritchey M,Yoshida Y,Bush CA,Cisar JO

    更新日期:2009-03-01 00:00:00

  • Molecular characterization and lytic activities of Streptococcus agalactiae bacteriophages and determination of lysogenic-strain features.

    abstract::The application of mitomycin C induction to 114 genetically diverse Streptococcus agalactiae strains generated 36 phage suspensions. On electron microscopy of the phage suspensions, it was possible to assign the phages to the Siphoviridae family, with three different morphotypes (A, B, and C). Phage genetic diversity ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00426-09

    authors: Domelier AS,van der Mee-Marquet N,Sizaret PY,Héry-Arnaud G,Lartigue MF,Mereghetti L,Quentin R

    更新日期:2009-08-01 00:00:00

  • Elucidation of an alternate isoleucine biosynthesis pathway in Geobacter sulfurreducens.

    abstract::The central metabolic model for Geobacter sulfurreducens included a single pathway for the biosynthesis of isoleucine that was analogous to that of Escherichia coli, in which the isoleucine precursor 2-oxobutanoate is generated from threonine. 13C labeling studies performed in G. sulfurreducens indicated that this pat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01841-07

    authors: Risso C,Van Dien SJ,Orloff A,Lovley DR,Coppi MV

    更新日期:2008-04-01 00:00:00

  • Aeromonas salmonicida possesses two genes encoding homologs of the major outer membrane protein, OmpA.

    abstract::Two homologs of the outer membrane protein OmpA were identified in Aeromonas salmonicida by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting, and amino-terminal sequence analyses. An A. salmonicida genomic DNA library was constructed by using lambda GEM-11 and recombinant phage carrying both g...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.6.1623-1630.1996

    authors: Costello GM,Vipond R,MacIntyre S

    更新日期:1996-03-01 00:00:00

  • cis-acting sites in the transcript of the Bacillus subtilis trp operon regulate expression of the operon.

    abstract::Transcription of the trp operon of Bacillus subtilis is regulated by attenuation. A trpE'-'lacZ gene fusion preceded by the wild-type trp promoter-leader region was used to analyze regulation. Overproduction of the trp leader transcript in trans from a multicopy plasmid caused constitutive expression of the chromosoma...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.7.3080-3088.1988

    authors: Kuroda MI,Henner D,Yanofsky C

    更新日期:1988-07-01 00:00:00

  • Different roles of DosS and DosT in the hypoxic adaptation of Mycobacteria.

    abstract::The DosS (DevS) and DosT histidine kinases form a two-component system together with the DosR (DevR) response regulator in Mycobacterium tuberculosis. DosS and DosT, which have high sequence similarity to each other over the length of their amino acid sequences, contain two GAF domains (GAF-A and GAF-B) in their N-ter...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00550-10

    authors: Kim MJ,Park KJ,Ko IJ,Kim YM,Oh JI

    更新日期:2010-10-01 00:00:00

  • Evidence for the existence of two arginyl-transfer ribonucleic acid synthetase activities in Escherichia coli.

    abstract::Two arginyl-transfer ribonucleic acid (tRNA) synthetase (EC 6.1.1.13, arginine: ribonucleic acid ligase adenosine monophosphate) activities were found in extracts of Escherichia coli strains AB1132 and NP2. The two arginyl-tRNA synthetase activities in extracts of strain AB1132 were found to be separable by diethylami...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.113.2.891-894.1973

    authors: Yem DW,Williams LS

    更新日期:1973-02-01 00:00:00

  • Overproduction of tryptophanyl-tRNA synthetase relieves transcription termination at the Escherichia coli tryptophan operon attenuator.

    abstract::Overproduction of tryptophanyl-tRNA synthetase increased trp operon expression by reducing transcription termination at the trp attenuator. The total cellular level of charged tRNATrp was not affected by increased levels of the synthetase. We propose that excess synthetase binds charged tRNATrp and reduces the concent...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.160.2.805-807.1984

    authors: Das A,Yanofsky C

    更新日期:1984-11-01 00:00:00

  • GLYCINE SYNTHESIS AND METABOLISM IN ESCHERICHIA COLI.

    abstract::Pizer, Lewis I. (University of Pennsylvania, Philadelphia). Glycine synthesis and metabolism in Escherichia coli. J. Bacteriol. 89:1145-1150. 1965.-A correlation was demonstrated between a nutritional requirement that can only be satisfied by glycine and the absence of the enzymatic capacity to interconvert l-serine a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.89.4.1145-1150.1965

    authors: PIZER LI

    更新日期:1965-04-01 00:00:00

  • Chemical and biochemical changes in subcellular fractions of guinea pig liver during infection with Coxiella burneti.

    abstract::Livers of uninfected guinea pigs and of guinea pigs infected with Coxiella burneti were fractionated into smooth endoplasmic reticulum, rough endoplasmic reticulum (RER), pellet, and cell sap fractions. The ribonucleic acid (RNA) and protein of each fraction were determined, and the phosphorylase, glucose-6-phosphatas...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.102.2.334-340.1970

    authors: Paretsky D,Stueckemann J

    更新日期:1970-05-01 00:00:00

  • Mechanism of chloride elimination from 3-chloro- and 2,4-dichloro-cis,cis-muconate: new insight obtained from analysis of muconate cycloisomerase variant CatB-K169A.

    abstract::Chloromuconate cycloisomerases of bacteria utilizing chloroaromatic compounds are known to convert 3-chloro-cis,cis-muconate to cis-dienelactone (cis-4-carboxymethylenebut-2-en-4-olide), while usual muconate cycloisomerases transform the same substrate to the bacteriotoxic protoanemonin. Formation of protoanemonin req...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.15.4551-4561.2001

    authors: Kaulmann U,Kaschabek SR,Schlömann M

    更新日期:2001-08-01 00:00:00

  • A quorum-sensing system in the free-living photosynthetic bacterium Rhodobacter sphaeroides.

    abstract::Rhodobacter sphaeroides is a free-living, photoheterotrophic bacterium known for its genomic and metabolic complexity. We have discovered that this purple photosynthetic organism possesses a quorum-sensing system. Quorum sensing occurs in a number of eukaryotic host-associated gram-negative bacteria. In these bacteria...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.23.7530-7537.1997

    authors: Puskas A,Greenberg EP,Kaplan S,Schaefer AL

    更新日期:1997-12-01 00:00:00

  • Transcriptional analysis of pqqD and study of the regulation of pyrroloquinoline quinone biosynthesis in Methylobacterium extorquens AM1.

    abstract::Methanol dehydrogenase, the enzyme that oxidizes methanol to formaldehyde in gram-negative methylotrophs, contains the prosthetic group pyrroloquinoline quinone (PQQ). To begin to analyze how the synthesis of PQQ is coordinated with the production of other methanol dehydrogenase components, the transcription of one of...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.1.206-211.1995

    authors: Ramamoorthi R,Lidstrom ME

    更新日期:1995-01-01 00:00:00

  • Identification of a novel nutrient-sensing histidine kinase/response regulator network in Escherichia coli.

    abstract::When carbon sources become limiting for growth, bacteria must choose which of the remaining nutrients should be used first. We have identified a nutrient-sensing signaling network in Escherichia coli that is activated at the transition to stationary phase. The network is composed of the two histidine kinase/response r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01554-14

    authors: Behr S,Fried L,Jung K

    更新日期:2014-06-01 00:00:00

  • A novel two-component signaling system that activates transcription of an enterohemorrhagic Escherichia coli effector involved in remodeling of host actin.

    abstract::Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is responsible for worldwide outbreaks of bloody diarrhea, hemorrhagic colitis, and life-threatening hemolytic uremic syndrome. After colonizing the large intestine, EHEC forms attaching and effacing (AE) lesions on intestinal epithelial cells. These lesions cause dest...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01848-06

    authors: Reading NC,Torres AG,Kendall MM,Hughes DT,Yamamoto K,Sperandio V

    更新日期:2007-03-01 00:00:00

  • Regulation of the cadA cadmium resistance determinant of Staphylococcus aureus plasmid pI258.

    abstract::Regulation of the cadA cadmium and zinc resistance determinant of Staphylococcus aureus plasmid pI258 was demonstrated by using gene fusions and direct measurements of transcription. In growth experiments, cells harboring the intact cadA operon were induced with different cations and challenged by an inhibitory concen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.23.7643-7649.1991

    authors: Yoon KP,Misra TK,Silver S

    更新日期:1991-12-01 00:00:00

  • Colcemid sensitivity of fission yeast: permeability and detoxification properties of resistant mutants.

    abstract::Mutants of Schizosaccharomyces pombe, which were resistant to concentrations of colcemid inhibitory to the wild-type parent, were not diminished in their permeability to colcemid or colchicine. They also did not modify colcemid to a detectable derivative or to a product that was incapable of binding to brain tubulin. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.129.1.198-201.1977

    authors: Lederberg S,Gourse RL,Sackett DL

    更新日期:1977-01-01 00:00:00

  • Role of adaptor TrfA and ClpPC in controlling levels of SsrA-tagged proteins and antitoxins in Staphylococcus aureus.

    abstract::Staphylococcus aureus responds to changing extracellular environments in part by adjusting its proteome through alterations of transcriptional priorities and selective degradation of the preexisting pool of proteins. In Bacillus subtilis, the proteolytic adaptor protein MecA has been shown to play a role in assisting ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02222-14

    authors: Donegan NP,Marvin JS,Cheung AL

    更新日期:2014-12-01 00:00:00

  • The ars operon in the skin element of Bacillus subtilis confers resistance to arsenate and arsenite.

    abstract::The Bacillus subtilis skin element confers resistance to arsenate and arsenite. The ars operon in the skin element contains four genes in the order arsR, ORF2, arsB, and arsC. Three of these genes are homologous to the arsR, arsB, and arsC genes from the staphylococcal plasmid pI258, while no homologs of ORF2 have bee...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.7.1655-1661.1998

    authors: Sato T,Kobayashi Y

    更新日期:1998-04-01 00:00:00

  • EutR is a direct regulator of genes that contribute to metabolism and virulence in enterohemorrhagic Escherichia coli O157:H7.

    abstract::Ethanolamine (EA) metabolism is a trait associated with enteric pathogens, including enterohemorrhagic Escherichia coli O157:H7 (EHEC). EHEC causes severe bloody diarrhea and hemolytic uremic syndrome. EHEC encodes the ethanolamine utilization (eut) operon that allows EHEC to metabolize EA and gain a competitive advan...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00937-13

    authors: Luzader DH,Clark DE,Gonyar LA,Kendall MM

    更新日期:2013-11-01 00:00:00

  • A family of insertion sequences that impacts integrons by specific targeting of gene cassette recombination sites, the IS1111-attC Group.

    abstract::Integrons facilitate the evolution of complex phenotypes by physical and transcriptional linkage of genes. They can be categorized as chromosomal integrons (CIs) or mobile resistance integrons (MRIs). The significance of MRIs for the problem of multiple antibiotic resistance is well established. CIs are more widesprea...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00229-08

    authors: Tetu SG,Holmes AJ

    更新日期:2008-07-01 00:00:00

  • Characterization of Proteus mirabilis precocious swarming mutants: identification of rsbA, encoding a regulator of swarming behavior.

    abstract::Proteus mirabilis swarming behavior is characterized by the development of concentric rings of growth that are formed as cyclic events of swarmer cell differentiation, swarming migration, and cellular differentiation are repeated during colony translocation across a surface. This cycle produces the bull's-eye colony o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:

    authors: Belas R,Schneider R,Melch M

    更新日期:1998-12-01 00:00:00

  • Carbohydrate Accumulation and Metabolism in Escherichia coli: Characteristics of the Reversions of ctr Mutations.

    abstract::The reversion behavior of pleiotropic carbohydrate mutants, previously designated as ctr, was studied. The mutants revert to complete restoration of the wild-type phenotype, as well as to a spectrum of partial wild-type phenotypes. Lac(+) reversions were found in the lac region (11 min) and some Mal(+) reversions occu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.104.3.1318-1324.1970

    authors: Wang RJ,Morse HG,Morse ML

    更新日期:1970-12-01 00:00:00

  • A Type Ib ParB protein involved in plasmid partitioning in a gram-positive bacterium.

    abstract::Our current understanding of segregation of prokaryotic plasmids has been derived mainly from the study of the gram-negative bacterial plasmids. We previously reported a replicon of the cryptic plasmid from a gram-positive bacterium, Leifsonia xyli subsp. cynodontis. The replicon contains a putative plasmid partition ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01232-06

    authors: Yin P,Li TY,Xie MH,Jiang L,Zhang Y

    更新日期:2006-12-01 00:00:00

  • Mutagenesis of Neisseria gonorrhoeae: absence of error-prone repair.

    abstract::The lethal and mutagenic effects of various mutagens on Neisseria gonorrhoeae were investigated. Lethality studies demonstrated that N. gonorrhoeae was relatively sensitive to ethyl methanesulfonate, UV light, and methyl methanesulfonate. Although N. gonorrhoeae was readily mutated by ethyl methanesulfonate and N-meth...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.160.1.288-293.1984

    authors: Campbell LA,Yasbin RE

    更新日期:1984-10-01 00:00:00

  • Antibiotic production by myxobacteria plays a role in predation.

    abstract::Myxobacteria are predatory and are prolific producers of secondary metabolites. Here, we tested a hypothesized role that secondary metabolite antibiotics function as weapons in predation. To test this, a Myxococcus xanthus Δta1 mutant, blocked in antibiotic TA (myxovirescin) production, was constructed. This TA(-) mut...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05052-11

    authors: Xiao Y,Wei X,Ebright R,Wall D

    更新日期:2011-09-01 00:00:00

  • Cloning and characterization of the hemolysin determinants from Vibrio cholerae RV79(Hly+), RV79(Hly-), and 569B.

    abstract::The Hly region from the chromosome of Vibrio cholerae El Tor strain RV79(Hly-) and the nonhemolytic classical strain 569B were cloned into plasmid vector pBR322. Escherichia coli K-12 transformants possessing these recombinant plasmids were nonhemolytic and were detected with a 32P-labeled hly-specific DNA probe. Rest...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.162.1.35-41.1985

    authors: Goldberg SL,Murphy JR

    更新日期:1985-04-01 00:00:00