Purification and properties of phytate-specific phosphatase from Bacillus subtilis.

Abstract:

:An enzyme which liberates Pi from myo-inositol hexaphosphate (phytic acid) was shown to be present in culture filtrates of Bacillus subtilis. It was purified until it was homogeneous by ultracentrifugation, but it still showed two isozymes on polyacrylamide gel electrophoresis. The enzyme differed from other previously known phytases in its metal requirement and in its specificity for phytate. It had a specific requirement for Ca2+ for its activity. The enzyme hydrolyzed only phytate and had no action on other phosphate esters tested. This B. subtilis phytase is the only known phytate-specific phosphatase. The products of hydrolysis of phytate by this enzyme were Pi and myo-inositol monophosphate. The enzyme showed optimum activity at pH 7.5. It was inhibited by Ba2+, Sr2+, Hg2+, Cd2+, and borate. Its activity was unaffected by urea, diisopropylfluorophosphate, arsenate, fluoride, mercaptoethanol, trypsin, papain, and elastase.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Powar VK,Jagannathan V

doi

10.1128/JB.151.3.1102-1108.1982

subject

Has Abstract

pub_date

1982-09-01 00:00:00

pages

1102-8

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

151

pub_type

杂志文章
  • The ams-1 and rne-3071 temperature-sensitive mutations in the ams gene are in close proximity to each other and cause substitutions within a domain that resembles a product of the Escherichia coli mre locus.

    abstract::Two temperature-sensitive mutations, ams-1 and rne-3071, in the ams (altered mRNA stability) gene have been used extensively to investigate the processing and decay of RNA in Escherichia coli. We have sequenced these temperature-sensitive alleles and found that the mutations are separated by only 6 nucleotides and cau...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.13.4245-4249.1993

    authors: McDowall KJ,Hernandez RG,Lin-Chao S,Cohen SN

    更新日期:1993-07-01 00:00:00

  • The ferrichrome uptake pathway in Pseudomonas aeruginosa involves an iron release mechanism with acylation of the siderophore and recycling of the modified desferrichrome.

    abstract::The uptake of iron into Pseudomonas aeruginosa is mediated by two major siderophores produced by the bacterium, pyoverdine and pyochelin. The bacterium is also able of utilize several heterologous siderophores of bacterial or fungal origin. In this work, we have investigated the iron uptake in P. aeruginosa PAO1 by th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01539-09

    authors: Hannauer M,Barda Y,Mislin GL,Shanzer A,Schalk IJ

    更新日期:2010-03-01 00:00:00

  • Acetylation of the response regulator RcsB controls transcription from a small RNA promoter.

    abstract::Nε-lysine acetylation was recently discovered on many bacterial proteins that function in diverse cellular processes. Thus, many questions remain unanswered. For example, what mechanisms regulate lysine acetylation? Does acetylation affect physiology? To help answer these questions, we studied the Escherichia coli res...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00383-13

    authors: Hu LI,Chi BK,Kuhn ML,Filippova EV,Walker-Peddakotla AJ,Bäsell K,Becher D,Anderson WF,Antelmann H,Wolfe AJ

    更新日期:2013-09-01 00:00:00

  • Role of SpoVG in asymmetric septation in Bacillus subtilis.

    abstract::Deletion of the citC gene, coding for isocitrate dehydrogenase, arrests sporulation of Bacillus subtilis at stage I after bipolar localization of the cell division protein FtsZ but before formation of the asymmetric septum. A spontaneous extragenic suppressor mutation that overcame the stage I block was found to map w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.11.3392-3401.1999

    authors: Matsuno K,Sonenshein AL

    更新日期:1999-06-01 00:00:00

  • BIOLOGY OF BUDDING BACTERIA. 3. FINE STRUCTURE OF RHODOMICROBIUM AND HYPHOMICROBIUM SPP.

    abstract::Conti, S. F. (Dartmouth Medical School, Hanover, N.H.), and Peter Hirsch. Biology of budding bacteria. III. Fine structure of Rhodomicrobium and Hyphomicrobium spp. J. Bacteriol. 89:503-512. 1965.-The ultrastructure of 14 strains of hyphomicrobia, and of Rhodomicrobium vannielii, was investigated by means of electron ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.89.2.503-512.1965

    authors: CONTI SF,HIRSCH P

    更新日期:1965-02-01 00:00:00

  • Regulation of spo0H, a gene coding for the Bacillus subtilis sigma H factor.

    abstract::The Bacillus spo0H gene codes for sigma H, which, as part of the RNA polymerase holoenzyme E sigma H, is responsible for the transcription of several genes which are expressed at the beginning of the sporulation process. In this communication, we examined the regulation of the spo0H gene of Bacillus subtilis by using ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.2.521-529.1991

    authors: Weir J,Predich M,Dubnau E,Nair G,Smith I

    更新日期:1991-01-01 00:00:00

  • Denaturation mapping of R factor deoxyribonucleic acid.

    abstract::The R factor NR1 consists of two components: a resistance transfer factor which harbors the tetracycline resistance genes (RTF-TC) and the r-determinants component which harbors the other drug resistance genes. Using partial denaturation mapping it is possible to distinguish the RTF-TC region from the r-determinants r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.123.3.1035-1042.1975

    authors: Perlman D,Twose TM,Holland MJ,Rownd RH

    更新日期:1975-09-01 00:00:00

  • Separation of beta-factor synthesis from stimulated beta-carotene synthesis in mated cultures of Blakeslea trispora.

    abstract::Mated cultures of Blakeslea trispora, grown in a potato extract-glucose-thiamine medium, produced 10 to 15 times more beta-carotene than either unmated culture. Mated, but not unmated, cultures produced a family of compounds (beta factor) which stimulated carotenogenesis in unmated cultures. In fact, carotenogenesis w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.2.426-432.1968

    authors: Sutter RP,Rafelson ME Jr

    更新日期:1968-02-01 00:00:00

  • Complete genome sequence of Burkholderia sp. Strain GG4, a betaproteobacterium that reduces 3-oxo-N-acylhomoserine lactones and produces different N-acylhomoserine lactones.

    abstract::Burkholderia sp. strain GG4, isolated from the ginger rhizosphere, possesses a unique N-acylhomoserine lactone (AHL)-modifying activity that reduces 3-oxo-AHLs to 3-hydroxy-AHLs. To the best of our knowledge, this is the first sequenced genome from a bacterium of the genus Burkholderia that shows both quorum-sensing a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01578-12

    authors: Hong KW,Koh CL,Sam CK,Yin WF,Chan KG

    更新日期:2012-11-01 00:00:00

  • Abnormal excision and transfer of chromosomal segments by a strain of Escherichia coli K-12.

    abstract::PB15 is an Hfr strain of Escherichia coli K-12. It arose from an F' strain carrying a temperature-sensitive F-gal by an event which blocked the detachment of F-gal in the normally reversible integration process. In PB15, the detachment of F-gal by a second mechanism can now be detected: this mechanism results in the e...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.111.1.119-128.1972

    authors: Bergquist PL,Adelberg EA

    更新日期:1972-07-01 00:00:00

  • Translation of trpG in Bacillus subtilis is regulated by the trp RNA-binding attenuation protein (TRAP).

    abstract::trpG of Bacillus subtilis encodes a glutamine amidotransferase subunit that is involved in the synthesis of both folic acid and L-tryptophan. Expression of trpG is negatively regulated by tryptophan even though this gene is located within a folic acid biosynthetic operon. Examination of both transcriptional and transl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.15.4272-4278.1995

    authors: Yang M,de Saizieu A,van Loon AP,Gollnick P

    更新日期:1995-08-01 00:00:00

  • Cloning and expression in Escherichia coli of an extremely thermostable oligo-1,6-glucosidase gene from Bacillus thermoglucosidasius.

    abstract::The gene for an extremely thermostable oligo-1,6-glucosidase (dextrin-6-alpha-D-glucanohydrolase; EC 3.2.1.10) of obligately thermophilic Bacillus thermoglucosidasius KP1006 was cloned within a 4.2-kilobase HindIII-PvuII fragment of DNA by using the plasmid pUC19 as a vector and Escherichia coli C600 as a host. The ge...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.2.1219-1222.1989

    authors: Watanabe K,Iha H,Ohashi A,Suzuki Y

    更新日期:1989-02-01 00:00:00

  • Genome sequence of Reyranella massiliensis, a bacterium associated with amoebae.

    abstract::Reyranella massiliensis is an Alphaproteobacterium member of the class Rhodospirillaceae, growing in amoebae. We sequenced the genome of type strain 521(T). It is composed of a 5,792,218-bp chromosome and encodes 5,675 protein-coding genes and 53 RNA genes, including 3 rRNA genes. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01260-12

    authors: Pagnier I,Croce O,Robert C,Raoult D,La Scola B

    更新日期:2012-10-01 00:00:00

  • Molecular cloning and characterization of the genes (pbpA and rodA) responsible for the rod shape of Escherichia coli K-12: analysis of gene expression with transposon Tn5 mutagenesis and protein synthesis directed by constructed plasmids.

    abstract::Two cell shape-determining genes of Escherichia coli K-12, pbpA, the structural gene for penicillin-binding protein 2, and rodA, whose protein is unknown, were subcloned into plasmid vectors from the transducing phage lambda MAd lip24, which carries the lip-leuS region of the E. coli chromosome. Plasmids with restrict...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.154.1.10-16.1983

    authors: Asoh S,Matsuzawa H,Matsuhashi M,Ohta T

    更新日期:1983-04-01 00:00:00

  • Spontaneous missense mutations in the rplX gene for ribosomal protein L24 from Escherichia coli.

    abstract::Temperature-resistant pseudorevertants of the temperature-sensitive Escherichia coli mutant KNS19, harboring a mutation in rplX, the gene for ribosomal protein L24, were isolated, cloned, and sequenced. The codon GAC for the amino acid Asp in the temperature-sensitive mutant corresponding to position 84 in the protein...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.10.4854-4856.1987

    authors: Nishi K,Müller M,Schnier J

    更新日期:1987-10-01 00:00:00

  • DNA damage-inducible loci in Salmonella typhimurium.

    abstract::lac operon fusions to DNA damage-inducible (din) loci were generated in Salmonella typhimurium LT2. Many of these din fusions were efficiently repressed by cloned Escherichia coli LexA, while others were not; all required RecA for induction. Several din fusions exhibited strong inducibility and will be useful in devel...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.11.3587-3590.1991

    authors: Smith CM,Arany Z,Orrego C,Eisenstadt E

    更新日期:1991-06-01 00:00:00

  • Factors affecting cellulolysis by Ruminococcus albus.

    abstract::The factors influencing the digestion of pebble-milled cellulose by enzymes were studied by using several strains of Ruminococcus albus including a mutant characterized by a more eccentric location of its colony in the clearing produced by digestion of the cellulose in the thin layer lining the wall of a culture tube....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.114.2.729-737.1973

    authors: Smith WR,Yu I,Hungate RE

    更新日期:1973-05-01 00:00:00

  • A 53-base-pair inverted repeat negatively regulates expression of the adjacent and divergently oriented cytochrome P450(BM-1) gene and its regulatory gene, bm1P1, in Bacillus megaterium.

    abstract::To study the role of the cis-acting element(s) in controlling the expression of the cytochrome P450(BM-1) gene and its upstream regulatory gene, bm1P1, in Bacillus megaterium, various deletion derivatives were constructed. A 53-bp inverted repeat located midway between the P450(BM-1) gene and bm1P1 gene was found in v...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.1.280-283.1997

    authors: Shaw GC,Sung CC,Liu CH,Kao HS

    更新日期:1997-01-01 00:00:00

  • Genes responsible for the supervirulence phenotype of Agrobacterium tumefaciens A281.

    abstract::Agrobacterium tumefaciens A281 induces large, rapidly appearing tumors on a variety of plants and has a wider host range than other strains of A. tumefaciens. By using Tn3HoHo1 transposon mutagenesis and complementation analysis, a 2.5-kilobase DNA fragment which is responsible for the supervirulence phenotype was ide...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.10.4417-4425.1987

    authors: Jin SG,Komari T,Gordon MP,Nester EW

    更新日期:1987-10-01 00:00:00

  • Dependence of Escherichia coli hyperbaric oxygen toxicity on the lipid acyl chain composition.

    abstract::This study examines certain membrane-related aspects of oxygen poisoning in Escherichia coli K1060 (fabB fadE lacI) and its parent strain, K-12 Ymel. Cells were grown to exponential or stationary phase in a minimal medium and exposed to air plus 300 lb/in2 of O2 as a suspension in minimal salts. After an initial lag, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.134.3.808-820.1978

    authors: Harley JB,Santangelo GM,Rasmussen H,Goldfine H

    更新日期:1978-06-01 00:00:00

  • Structure-specific nuclease activities of Pyrococcus abyssi RNase HII.

    abstract::Faithful DNA replication involves the removal of RNA residues from genomic DNA prior to the ligation of nascent DNA fragments in all living organisms. Because the physiological roles of archaeal type 2 RNase H are not fully understood, the substrate structure requirements for the detection of RNase H activity need fur...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00268-10

    authors: Le Laz S,Le Goaziou A,Henneke G

    更新日期:2010-07-01 00:00:00

  • Analysis of the growth of recombination-deficient strains of Escherichia coli K-12.

    abstract::Cultures of recombination-deficient strains of Escherichia coli are composed of three classes of cells: (i) viable cells, which can undergo 20 or more generations, (ii) residually dividing cells, which can undergo fewer than 20 generations (probably an average of fewer than 6), and (iii) nondividing cells, which are i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.118.1.242-249.1974

    authors: Capaldo FN,Ramsey G,Barbour SD

    更新日期:1974-04-01 00:00:00

  • Dark-recovery processes in Escherichia coli irradiated with ultraviolet light. 3. Effect of rec mutations on recovery of excision-deficient mutants of Escherichia coli K-12.

    abstract::Mutants of Escherichia coli K-12 unable to excise pyrimidine dimers from their deoxyribonucleic acid (DNA) because of a uvr mutation show a higher survival when plated on a minimal salts medium after exposure to ultraviolet radiation than when plated on a complex medium such as nutrient agar containing yeast extract. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.102.2.404-410.1970

    authors: Ganesan AK,Smith KC

    更新日期:1970-05-01 00:00:00

  • QUANTITATIVE STUDY OF ENDOLYSIN SYNTHESIS DURING REPRODUCTION OF LAMBDA PHAGES.

    abstract::Groman, Neal B. (University of Washington, Seattle) and Grace Suzuki. Quantitative study of endolysin synthesis during reproduction of lambda phages. J. Bacteriol. 86:187-194. 1963.-Endolysin is presumed to be a phage-induced enzyme participating in lysis through its destructive action on the host cell wall. A method ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.86.2.187-194.1963

    authors: GROMAN NB,SUZUKI G

    更新日期:1963-08-01 00:00:00

  • Electron transport in the pathway of acetate conversion to methane in the marine archaeon Methanosarcina acetivorans.

    abstract::A liquid chromatography-hybrid linear ion trap-Fourier transform ion cyclotron resonance mass spectrometry approach was used to determine the differential abundance of proteins in acetate-grown cells compared to that of proteins in methanol-grown cells of the marine isolate Methanosarcina acetivorans metabolically lab...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.2.702-710.2006

    authors: Li Q,Li L,Rejtar T,Lessner DJ,Karger BL,Ferry JG

    更新日期:2006-01-01 00:00:00

  • Role of the ganSPQAB Operon in Degradation of Galactan by Bacillus subtilis.

    abstract::Bacillus subtilis possesses different enzymes for the utilization of plant cell wall polysaccharides. This includes a gene cluster containing galactan degradation genes (ganA and ganB), two transporter component genes (ganQ and ganP), and the sugar-binding lipoprotein-encoding gene ganS (previously known as cycB). The...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00468-16

    authors: Watzlawick H,Morabbi Heravi K,Altenbuchner J

    更新日期:2016-09-22 00:00:00

  • Caulobacter flagellar organelle: synthesis, compartmentation, and assembly.

    abstract::In Caulobacter crescentus biogenesis of the flagellar organelle occurs during one stage of its complex life cycle. Thus in synchronous cultures it is possible to assay the sequential synthesis and assembly of the flagellum and hook in vivo with a combination of biochemical and radioimmunological techniques. The period...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.135.3.1062-1069.1978

    authors: Lagenaur C,Agabian N

    更新日期:1978-09-01 00:00:00

  • Genetic and physical analyses of Caulobacter crescentus trp genes.

    abstract::Caulobacter crescentus trp mutants were identified from a collection of auxotrophs. Precursor feeding experiments, accumulation studies, and complementation experiments resulted in the identification of six genes corresponding to trpA, trpB, trpC, trpD, trpE, and trpF. Genetic mapping experiments demonstrated that the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.160.1.279-287.1984

    authors: Winkler ME,Schoenlein PV,Ross CM,Barrett JT,Ely B

    更新日期:1984-10-01 00:00:00

  • Characterization of the gene for a protein kinase which phosphorylates the sporulation-regulatory proteins Spo0A and Spo0F of Bacillus subtilis.

    abstract::The kinA (spoIIJ) locus contains a single gene which codes for a protein of 69,170 daltons showing strong homology to the transmitter kinases of two component regulatory systems. The purified kinase autophosphorylates in the presence of ATP and mediates the transfer of phosphate to the Spo0A and Spo0F sporulation regu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.11.6187-6196.1989

    authors: Perego M,Cole SP,Burbulys D,Trach K,Hoch JA

    更新日期:1989-11-01 00:00:00

  • Antibody response to Staphylococcus aureus in rabbits: sequence of immunoglobulin synthesis and its correlation with passive protection in mice.

    abstract::Antibody in hyperimmune rabbit antisera specific for Staphylococcus aureus teichoic acid was shown to be associated with the IgM fraction. Treatment of such sera with mercaptoethanol destroyed its activity in passive mouse protection tests, whereas absorption with antirabbit IgG had no effect. Antibody response in nor...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.96.5.1540-1545.1968

    authors: Yoshida K,Ekstedt RD

    更新日期:1968-11-01 00:00:00