QUANTITATIVE STUDY OF ENDOLYSIN SYNTHESIS DURING REPRODUCTION OF LAMBDA PHAGES.

abstract：:The biodegradative threonine dehydratase (tdc) operon was mapped at 68 min on the Escherichia coli K-12 chromosome. The order of markers in the clockwise direction was dnaG uxaA tdc argG. A tdc deletion was isolated and mapped to this region of the chromosome. By using a tdcB-lacZ fusion the clockwise direction of tra...

journal_title：Journal of bacteriology

authors： Schweizer HP,Datta P

更新日期：1988-11-01 00:00:00

abstract：:An increase in the apparent molecular weight of the principal outer membrane protein (POMP) of Neisseria gonorrhoeae is associated with introduction of the penB2 genetic marker, which results in low-level, relatively nonspecific antibiotic resistance. Limited proteolysis of the two forms of POMP showed that they had f...

journal_title：Journal of bacteriology

authors： Cannon JG,Klapper DG,Blackman EY,Sparling PF

更新日期：1980-08-01 00:00:00

abstract：:Streptomyces coelicolor Müller contains two superoxide dismutases (SODs), nickel-containing (NiSOD) and iron- and zinc-containing SOD (FeZnSOD). The sodF gene encoding FeZnSOD was isolated by using PCR primers corresponding to the N-terminal peptide sequence of the purified FeZnSOD and a C-terminal region conserved am...

journal_title：Journal of bacteriology

authors： Kim EJ,Chung HJ,Suh B,Hah YC,Roe JH

更新日期：1998-04-01 00:00:00

abstract：:In Salmonella enterica, the biosynthetic pathways for the generation of purines and the essential cofactor thiamine pyrophosphate branch after sharing five enzymatic steps. Phosphoribosyl amine (PRA) is the first intermediate in the common portion of the pathway and is generated from phosphoribosylpyrophosphate and gl...

journal_title：Journal of bacteriology

authors： Browne BA,Ramos AI,Downs DM

更新日期：2006-10-01 00:00:00

abstract：:An abundant 19.3-kDa Helicobacter pylori protein has been cloned, and the sequence is homologous with a ferritin-like protein produced by Escherichia coli K-12. Homologies are also present with a number of eucaryotic ferritins, as well as with the heme group-containing bacterioferritins. All amino acids involved in ch...

journal_title：Journal of bacteriology

authors： Frazier BA,Pfeifer JD,Russell DG,Falk P,Olsén AN,Hammar M,Westblom TU,Normark SJ

更新日期：1993-02-01 00:00:00

abstract：:Toxin-antitoxin (TA) systems are widely represented on mobile genetic elements as well as in bacterial chromosomes. TA systems encode a toxin and an antitoxin neutralizing it. We have characterized a homolog of the ccd TA system of the F plasmid (ccd(F)) located in the chromosomal backbone of the pathogenic O157:H7 Es...

journal_title：Journal of bacteriology

authors： Wilbaux M,Mine N,Guérout AM,Mazel D,Van Melderen L

更新日期：2007-04-01 00:00:00

abstract：:Structural analogs of S-adenosylhomocysteine were tested in vitro for inhibition of the yeast S-adenosylmethionine:delta 24-sterol-C-methyltransferase enzyme. A wide inhibitory range by these compounds was observed, suggesting which structural features of the parent compound are important for binding to the enzyme. No...

journal_title：Journal of bacteriology

authors： McCammon MT,Parks LW

更新日期：1981-01-01 00:00:00

abstract：:The broad-host-range plasmid RK2 is capable of replication and stable maintenance within a wide range of gram-negative bacterial hosts. It encodes the essential replication initiation protein TrfA, which binds to the host initiation protein, DnaA, at the plasmid origin of replication (oriV). There are two versions of ...

journal_title：Journal of bacteriology

authors： Kongsuwan K,Josh P,Picault MJ,Wijffels G,Dalrymple B

更新日期：2006-08-01 00:00:00

abstract：:Smith, Doyle C. (Kansas State University, Manhattan), V. D. Foltz, and T. H. Lord. Two lytic factors involved in induced synergistic hemolysis in normally nonhemolytic staphylococci. J. Bacteriol. 88:1700-1704. 1964.-Synergistic hemolysis of "normally nonhemolytic" staphylococci results from the combination of two fac...

journal_title：Journal of bacteriology

authors： SMITH DC,FOLTZ VD,LORD TH

更新日期：1964-12-01 00:00:00

abstract：:In an Escherichia coli B strain missing ribosomal protein L1, the synthesis rate of L11 is 50% greater than that of other ribosomal proteins. This finding is in agreement with the previous conclusion that L1 regulates synthesis of itself and L11 and indicates that this regulation is important for maintaining the balan...

journal_title：Journal of bacteriology

authors： Jinks-Robertson S,Nomura M

更新日期：1981-03-01 00:00:00

abstract：:Proteins synthesized in Escherichia coli during recovery from starvation were resolved by two-dimensional polyacrylamide gel electrophoresis. Nine outgrowth-specific proteins, which appeared in two kinetic groups, that were not detected in either starved or exponential-phase cells were synthesized. Five other proteins...

journal_title：Journal of bacteriology

authors： Siegele DA,Guynn LJ

更新日期：1996-11-01 00:00:00

abstract：:The major cold shock protein of Escherichia coli, CS7.4, is produced at a level of 13% of total protein synthesis upon a temperature shift from 37 to 10 degrees C. The transcription of its gene (cspA) was found to be tightly regulated and induced only at low temperature. In addition, the cspA mRNA was extremely unstab...

journal_title：Journal of bacteriology

authors： Tanabe H,Goldstein J,Yang M,Inouye M

更新日期：1992-06-01 00:00:00

abstract：:An unusual xylose isomerase produced by Thermoanaerobacterium strain JW/SL-YS 489 was purified 28-fold to gel electrophoretic homogeneity, and the biochemical properties were determined. Its pH optimum distinguishes this enzyme from all other previously described xylose isomerases. The purified enzyme had maximal acti...

journal_title：Journal of bacteriology

authors： Liu SY,Wiegel J,Gherardini FC

更新日期：1996-10-01 00:00:00

abstract：:DnaA is an AAA+ ATPase and the conserved replication initiator in bacteria. Bacteria control the timing of replication initiation by regulating the activity of DnaA. DnaA binds to multiple sites in the origin of replication (oriC) and is required for recruitment of proteins needed to load the replicative helicase. Dna...

journal_title：Journal of bacteriology

authors： Bonilla CY,Grossman AD

更新日期：2012-09-01 00:00:00

abstract：:Iron-sulfur flavoproteins (ISF) constitute a widespread family of redox-active proteins in anaerobic prokaryotes. Based on sequence homologies, their overall structure is expected to be similar to that of flavodoxins, but in addition to a flavin mononucleotide cofactor they also contain a cubane-type [4Fe:4S] cluster....

journal_title：Journal of bacteriology

authors： Andrade SL,Cruz F,Drennan CL,Ramakrishnan V,Rees DC,Ferry JG,Einsle O

更新日期：2005-06-01 00:00:00

abstract：:Apparent pK values were determined for a series of commercially available carboxylic acids in a search for a buffer suitable for bacterial studies at pH 7 in media free from nitrogen, sulfur, and phosphorus. Of the compounds possessing pK' values near pH 7,3,6-endomethylene-1,2,3,6-tetrahydrophthalic acid (EMTA) was c...

journal_title：Journal of bacteriology

authors： Mallette MF

更新日期：1967-08-01 00:00:00

abstract：:The gene responsible for the uncommon glutamate dehydrogenase (GDH) activity of Lactococcus lactis was identified and characterized. It encodes a GDH of family I that is mainly active in glutamate biosynthesis, is carried by a large plasmid, and is included, with functional cadmium resistance genes, in a remnant Tn3-l...

journal_title：Journal of bacteriology

authors： Tanous C,Chambellon E,Sepulchre AM,Yvon M

更新日期：2005-07-01 00:00:00

abstract：:The recA441 mutant of Escherichia coli, which has been thought to have thermoinducible constitutive RecA protease activity, is known to have two mutations within recA. We show here that the mutation that alters codon 38 actually confers temperature-independent constitutive protease activity; the second mutation in rec...

journal_title：Journal of bacteriology

authors： Wang WB,Tessman ES

更新日期：1985-07-01 00:00:00

abstract：:Spontaneous mutants that were resistant to zinc were isolated from Alcaligenes eutrophus CH34 containing either the native plasmid pMOL28 or a derivative derepressed for its self-transfer, pMOL50. With the cured plasmid-free derivative of CH34, strain AE104, such mutants were not detected. The mutations, which were sh...

journal_title：Journal of bacteriology

authors： Collard JM,Provoost A,Taghavi S,Mergeay M

更新日期：1993-02-01 00:00:00

abstract：:In Streptococcus mutans, enzyme II(scr) and sucrose-6-phosphate hydrolase are two important enzymes in the transport and metabolism of dietary sucrose. The scr regulon of S. mutans is composed of three genes, scrA and scrB, which code for enzyme II(scr) and sucrose-6-phosphate hydrolase, respectively, and scrR, which ...

journal_title：Journal of bacteriology

authors： Wang B,Kuramitsu HK

更新日期：2003-10-01 00:00:00

abstract：:Cyanothece sp. strain ATCC 51142 is a unicellular, diazotrophic cyanobacterium which demonstrated extensive metabolic periodicities of photosynthesis, respiration, and nitrogen fixation when grown under N2-fixing conditions. N2 fixation and respiration peaked at 24-h intervals early in the dark or subjective-dark peri...

journal_title：Journal of bacteriology

authors： Colón-López MS,Sherman DM,Sherman LA

更新日期：1997-07-01 00:00:00

abstract：:D-Ribulose 1,5-bisphosphate (RuBP) carboxylase has been purified from the photosynthetic extreme halophile Ectothiorhodospira halophila. Despite a growth requirement for almost saturating sodium chloride in the medium, both crude and homogeneous preparations of RuBP carboxylase obtained from this organism were inhibit...

journal_title：Journal of bacteriology

authors： Tabita FR,McFadden BA

更新日期：1976-06-01 00:00:00

abstract：:The prevalence of the Moraxella catarrhalis immunoglobulin D (IgD)-binding outer membrane protein MID and its gene was determined in 91 clinical isolates and in 7 culture collection strains. Eighty-four percent of the clinical Moraxella strains expressed MID-dependent IgD binding. The mid gene was detected in all stra...

journal_title：Journal of bacteriology

authors： Möllenkvist A,Nordström T,Halldén C,Christensen JJ,Forsgren A,Riesbeck K

更新日期：2003-04-01 00:00:00

abstract：:The effect of heat shock on Myxococcus xanthus was investigated during both glycerol- and starvation-induced development. Cells heat shocked at 40 degrees C for 1 h prior to a development-inducing signal displayed an accelerated rate of myxospore formation at 30 degrees C. Additionally, M. xanthus cells heat shocked p...

journal_title：Journal of bacteriology

authors： Killeen KP,Nelson DR

更新日期：1988-11-01 00:00:00

abstract：:The formation of recombinants in Hfr crosses was studied in Escherichia coli strains carrying combinations of genes known to affect recombination and DNA repair. Mutations in ruv and recG eliminate activities that have been shown to process Holliday junction intermediates by nuclease cleavage and/or branch migration. ...

journal_title：Journal of bacteriology

authors： Ryder L,Whitby MC,Lloyd RG

更新日期：1994-03-01 00:00:00

abstract：:Ribulose 1,5-bisphosphate carboxylase-oxygenase (RuBPC-O) activity was greatly enhanced when Rhodopseudomonas sphaeroides was grown in a mineral salts medium supplied with 1.5% CO2 in hydrogen. Analysis of cell extracts by sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that cells growing on 1.5% C...

journal_title：Journal of bacteriology

authors： Jouanneau Y,Tabita FR

更新日期：1986-02-01 00:00:00

abstract：:Lambda Xis, which is required for site-specific excision of phage lambda from the bacterial chromosome, has a much shorter functional half-life than Int, which is required for both integration and excision (R. A. Weisberg and M. E. Gottesman, p. 489-500, in A. D. Hershey, ed., The Bacteriophage Lambda, 1971). We found...

journal_title：Journal of bacteriology

authors： Leffers GG Jr,Gottesman S

更新日期：1998-03-01 00:00:00

abstract：:By using the isotope pairs (22)Na-(24)Na and (42)K-(86)Rb, the uptake and retention of Na and K was studied in the salt-tolerant Debaryomyces hansenii and in the less tolerant Saccharomyces cerevisiae at NaCl levels of 4 mm and 0.68, 1.35, and 2.7 m in the medium. The ratio of K to Na is much higher in the cells than ...

journal_title：Journal of bacteriology

authors： Norkrans B,Kylin A

更新日期：1969-11-01 00:00:00

abstract：:The Rickettsia prowazekii sigma factor was overexpressed, purified, and used to reconstitute RNA polymerase holoenzyme species. R. prowazekii RNA polymerase-promoter complexes were unstable and remained dissociable and heparin sensitive under conditions in which the corresponding Escherichia coli complexes were not. T...

journal_title：Journal of bacteriology

authors： Aniskovitch LP,Winkler HH

更新日期：1995-11-01 00:00:00

abstract：:Monobromoacetate (MBA) is toxic for the 1,2-dichloroethane-degrading bacterium Xanthobacter autotrophicus GJ10 at concentrations higher than 5 mM. Mutants which are able to grow on higher concentrations of MBA were isolated and found to overexpress haloacid dehalogenase, which is encoded by the dhlB gene. In mutant GJ...

journal_title：Journal of bacteriology

authors： van der Ploeg J,Willemsen M,van Hall G,Janssen DB

更新日期：1995-03-01 00:00:00