Lambda Xis degradation in vivo by Lon and FtsH.

Abstract:

:Lambda Xis, which is required for site-specific excision of phage lambda from the bacterial chromosome, has a much shorter functional half-life than Int, which is required for both integration and excision (R. A. Weisberg and M. E. Gottesman, p. 489-500, in A. D. Hershey, ed., The Bacteriophage Lambda, 1971). We found that Xis is degraded in vivo by two ATP-dependent proteases, Lon and FtsH (HflB). Xis was stabilized two- to threefold more than in the wild type in a lon mutant and as much as sixfold more in a lon ftsH double mutant at the nonpermissive temperature for the ftsH mutation. Integration of lambda into the bacterial chromosome was delayed in the lon ftsH background, suggesting that accumulation of Xis in vivo interferes with integration. Overexpression of Xis in wild-type cells from a multicopy plasmid inhibited integration of lambda and promoted curing of established lysogens, confirming that accumulation of Xis interferes with the ability of Int to establish and maintain an integrated prophage.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Leffers GG Jr,Gottesman S

doi

10.1128/JB.180.6.1573-1577.1998

subject

Has Abstract

pub_date

1998-03-01 00:00:00

pages

1573-7

issue

6

eissn

0021-9193

issn

1098-5530

journal_volume

180

pub_type

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