Abstract:
:Streptomyces coelicolor Müller contains two superoxide dismutases (SODs), nickel-containing (NiSOD) and iron- and zinc-containing SOD (FeZnSOD). The sodF gene encoding FeZnSOD was isolated by using PCR primers corresponding to the N-terminal peptide sequence of the purified FeZnSOD and a C-terminal region conserved among known FeSODs and MnSODs. The deduced amino acid sequence exhibited highest similarity to Mn- and FeSODs from Propionibacterium shermanii and Mycobacterium spp. The transcription start site of the sodF gene was determined by primer extension. When the sodF gene was cloned in pIJ702 and introduced into Streptomyces lividans TK24, it produced at least 30 times more FeZnSOD than the control cells. We disrupted the sodF gene in S. lividans TK24 and found that the disruptant did not produce any FeZnSOD enzyme activity but produced more NiSOD. The expression of the cloned sodF gene in TK24 cells was repressed significantly by Ni, consistent with the regulation pattern in nonoverproducing cells. This finding suggests that the cloned sodF gene contains the cis-acting elements necessary for Ni regulation. When the sodF mRNA in S. coelicolor Muller cells was analyzed by S1 mapping of both 5' and 3' ends, we found that Ni caused a reduction in the level of monocistronic sodF transcripts. Ni did not affect the stability of sodF mRNA, indicating that it regulates transcription. S. lividans TK24 cells overproducing FeZnSOD became more resistant to oxidants such as menadione and lawsone than the control cells, suggesting the protective role of FeZnSOD. However, the sodF disruptant survived as well as the wild-type strain in the presence of these oxidants, suggesting the complementing role of NiSOD increased in the disruptant.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Kim EJ,Chung HJ,Suh B,Hah YC,Roe JHdoi
10.1128/JB.180.8.2014-2020.1998subject
Has Abstractpub_date
1998-04-01 00:00:00pages
2014-20issue
8eissn
0021-9193issn
1098-5530journal_volume
180pub_type
杂志文章abstract::The nucleotide messenger cyclic di-GMP (c-di-GMP) plays a central role in the regulation of motility, virulence, and biofilm formation in many pathogenic bacteria. EAL domain-containing phosphodiesterases are the major signaling proteins responsible for the degradation of c-di-GMP and maintenance of its cellular level...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00560-12
更新日期:2012-09-01 00:00:00
abstract::Examination of the ilvF locus at 54 min on the Escherichia coli K-12 chromosome revealed that it is a cryptic gene for expression of a valine-resistant acetohydroxy acid synthase (acetolactate synthase; EC 4.1.3.18) distinct from previously reported isozymes. A spontaneous mutation, ilvF663, yielded IlvF+ enzyme activ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.6.3060-3065.1990
更新日期:1990-06-01 00:00:00
abstract::A superfamily of transport proteins, which includes the lactose permease of Escherichia coli, contains a highly conserved motif, G-X-X-X-D/E-R/K-X-G-R/K-R/K, in the loops that connect transmembrane segments 2 and 3 and transmembrane segments 8 and 9. Previous analysis of this motif in the lactose permease (A. E. Jesse...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.8.2616-2622.1997
更新日期:1997-04-01 00:00:00
abstract::Holins are a diverse group of small integral membrane proteins elaborated by bacteriophages to lyse bacterial hosts and effect release of progeny phages in a precisely timed manner. Recently, the holin S gene of phage lambda was overexpressed and the holin protein was purified to homogeneity by means of an oligohistid...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.16.4199-4211.1998
更新日期:1998-08-01 00:00:00
abstract::We previously reported that the methicillin resistance gene mecA is carried by a novel type of mobile genetic element, SCCmec (staphylococcal cassette chromosome mec), in the chromosome of methicillin-resistant Staphylococcus aureus (MRSA). These elements are precisely excised from the chromosome and integrated into a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.9.2711-2722.2003
更新日期:2003-05-01 00:00:00
abstract::Sphingobium japonicum UT26 utilizes gamma-hexachlorocyclohexane (gamma-HCH) as its sole source of carbon and energy. In our previous studies, we cloned and characterized genes encoding enzymes for the conversion of gamma-HCH to beta-ketoadipate in UT26. In this study, we analyzed a mutant obtained by transposon mutage...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01883-06
更新日期:2007-05-01 00:00:00
abstract::Lornitzo, Frank A. (Veterans Administration Hospital, Madison, Wis.), and Dexter S. Goldman. Reversible effect of bicarbonate on the inhibition of mycobacterial and yeast transglucosylases by mycoribnin. J. Bacteriol. 89:1086-1091. 1965.-The transglucosylase which catalyzes the formation of trehalose-6-phosphate from ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.4.1086-1091.1965
更新日期:1965-04-01 00:00:00
abstract::Virgibacillus halodenitrificans 1806 is an endospore-forming halophilic bacterium isolated from salterns in Korea. Here, we report the draft genome sequence of V. halodenitrificans 1806, which may reveal the molecular basis of osmoadaptation and insights into carbon and anaerobic metabolism in moderate halophiles. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01280-12
更新日期:2012-11-01 00:00:00
abstract::The vir locus of Bordetella pertussis apparently encodes a trans-acting positive regulator that is required for the coordinate expression of genes associated with virulence: pertussis toxin, filamentous hemagglutinin (FHA), hemolysin, and adenylate cyclase toxin. DNA clones of vir and of genes required for the synthes...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.7.2904-2913.1988
更新日期:1988-07-01 00:00:00
abstract::Leuconostoc mesenteroides subsp. mesenteroides is one of the most predominant lactic acid bacterial groups during kimchi fermentation. Here, we report the complete genome sequence of L. mesenteroides subsp. mesenteroides J18, which was isolated from kimchi. The genome of the strain consists of a 1,896,561-bp chromosom...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06498-11
更新日期:2012-02-01 00:00:00
abstract::Carere, A., and Isabella Spada-Sermonti (Istituto Superiore di Sanità, Rome, Italy). Nutritional mutations and transduction by ultraviolet-inactivated phage in Staphylococcus aureus. J. Bacteriol. 88:226-232. 1964.-By use of a semisynthetic medium, various nutritional mutants of Staphylococcus aureus 80 were isolated....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.1.226-232.1964
更新日期:1964-07-01 00:00:00
abstract::Both alpha-tocopherolquinol and alpha-tocopherolquinone were found in 56 of 93 strains of microorganisms examined. Organisms that contained these compounds included the single example of a eucaryotic alga, a Euglena, and a cyanobacterium (blue-green alga), 22 of 32 genera of bacteria, and 9 genera of yeasts. In the ba...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.151.3.1397-1402.1982
更新日期:1982-09-01 00:00:00
abstract::The Methanococcus maripaludis energy-conserving hydrogenase B (Ehb) generates low potential electrons required for autotrophic CO(2) assimilation. To analyze the importance of individual subunits in Ehb structure and function, markerless in-frame deletions were constructed in a number of M. maripaludis ehb genes. Thes...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01446-09
更新日期:2010-08-01 00:00:00
abstract::The tyrT (tRNA(1TYr)) locus of Escherichia coli B differs structurally from that of K-12 strains by the absence of 2 of 3.14 terminal repeat sequences. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.8.2469-2470.1996
更新日期:1996-04-01 00:00:00
abstract::The three-dimensional structure of the nickel-containing hydrogenase from Thiocapsa roseopersicina has been determined at a resolution of 2 nm in the plane and 4 nm in the vertical direction by electron microscopy and computerized image processing on microcrystals of the enzyme. The enzyme forms a large ring-shaped co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.8.2576-2580.1991
更新日期:1991-04-01 00:00:00
abstract::Superoxide dismutase (SOD) and catalase (CAT) levels were determined for several aerobically grown halophilic vibrios and compared with those found in aerobically grown Escherichia coli K-12. The SOD levels ranged from 25 to 103.6 U/mg of protein for the vibrios compared with 44.6 U/mg of protein for E. coli. The CAT ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.134.2.375-380.1978
更新日期:1978-05-01 00:00:00
abstract::In Bacillus thuringiensis, a novel N-acetylmuramoyl-L-alanine amidase gene (named cwlB) was detected, and the CwlB protein was purified and characterized. Reverse transcription-PCR (RT-PCR) results indicated that cwlB and an upstream gene (named cwlA) formed one transcriptional unit. 5' rapid amplification of cDNA end...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00112-13
更新日期:2013-06-01 00:00:00
abstract::Alkaline phosphatase (APase) is induced as a culture enters stationary phase because of limiting phosphate. The results presented here show that expression of APase is regulated both negatively and positively. PhoP, a homolog of a family of bacterial transcription factors, and PhoR, a homolog of bacterial histidine pr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.12.3749-3756.1993
更新日期:1993-06-01 00:00:00
abstract::Bacillus anthracis, the causative agent of anthrax, replicates as chains of vegetative cells by regulating the separation of septal peptidoglycan. Surface (S)-layer proteins and associated proteins (BSLs) function as chain length determinants and bind to the secondary cell wall polysaccharide (SCWP). In this study, we...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02364-14
更新日期:2015-01-01 00:00:00
abstract::In Klebsiella pneumoniae, products of the nitrogen fixation nifLA operon regulate transcription of the other nif operons. NifA activates transcription by sigma54-holoenzyme. In vivo, NifL antagonizes the action of NifA under aerobic conditions or in the presence of combined nitrogen. In contrast to a previous report, ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.15.4679-4687.1996
更新日期:1996-08-01 00:00:00
abstract::Gene sequences encoding the enzymes UDP-N-acetylglucosamine enolpyruvyl transferase (MurA) from many bacterial sources were analyzed. It was shown that whereas gram-negative bacteria have only one murA gene, gram-positive bacteria have two distinct genes encoding these enzymes which have possibly arisen from gene dupl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.15.4146-4152.2000
更新日期:2000-08-01 00:00:00
abstract::Short-term labeling with radioactive thymidine led to inaccurate estimates of deoxyribonucleic acid synthesis at some growth rates in steady-state cultures of Escherichia coli B/r. Estimates were corrected in chemostat cultures by adding adenosine, a known inhibitor of thymidine phosphorylase. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.110.3.1208-1210.1972
更新日期:1972-06-01 00:00:00
abstract::Smith, P. B. (Communicable Disease Center, Atlanta, Ga.). Growth of staphylococci on mercuric chloride agar. J. Bacteriol. 84:1016-1019. 1962.-An attempt has been made to corroborate a report that "epidemic" strains of staphylococci are more resistant to mercuric salts than are "nonepidemic" strains. A comparison of m...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.5.1016-1019.1962
更新日期:1962-11-01 00:00:00
abstract::The carbon monoxide (CO) dehydrogenase activity band from Clostridium pasteurianum was shown to contain nickel by in situ activity staining of polyacrylamide gels. However, the majority of the nickel in cell extracts was found to electrophorese independently of CO dehydrogenase. Comparative analysis with Clostridium t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.2.561-566.1982
更新日期:1982-02-01 00:00:00
abstract::The gene encoding a 45-kDa protein (45K) of Chlamydia trachomatis serovar F was cloned, sequenced, and overexpressed in Escherichia coli. Alignment of the deduced peptide sequence with E. coli elongation factor Tu (EF-Tu) demonstrated 69% identity. The 45K was recognized by a Chlamydia genus-specific monoclonal antibo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.4.1184-1187.1994
更新日期:1994-02-01 00:00:00
abstract::Cells of Saccharomyces cerevisiae permeabilized by treatment with ether take up and incorporate exogenous deoxynucleoside triphosphate into deoxyribonucleic acid (DNA). With rho(+) strains, more than 95% of the product was mitochondrial DNA (mtDNA). This report characterizes ether-permeabilized yeast cells and describ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.140.2.333-341.1979
更新日期:1979-11-01 00:00:00
abstract::Pep 5 and nisin are cationic peptide antibiotics which in addition to their membrane-disruptive action induce autolysis in staphylococci. To investigate the mechanism of lysis induction, the influence of the peptides on the activity of the N-acetylmuramoyl-L-alanine amidase of Staphylococcus simulans 22 was studied. I...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.12.5452-5458.1987
更新日期:1987-12-01 00:00:00
abstract::Reference strains from 48 selected serovars representing eight species of Leptospira were examined by two polymerase chain reaction (PCR)-based strategies. First, mapped restriction site polymorphisms (MRSP) were examined in PCR products from portions of rrs (16S rRNA gene) and rrl (23S rRNA gene). Twenty MRSP and 2 l...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.4.973-981.1993
更新日期:1993-02-01 00:00:00
abstract::More than 98% of reported human listeriosis cases are caused by Listeria monocytogenes serotypes within lineages I and II. Serotypes within lineage III (4a and 4c) are commonly isolated from environmental and food specimens. We report the first complete genome sequence of a lineage III isolate, HCC23, which will be us...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05236-11
更新日期:2011-07-01 00:00:00
abstract::The interaction of Streptococcus pyogenes (group A streptococcus [GAS]) with its human host requires several surface proteins. In this study, we isolated mutations in a gene required for the surface localization of protein F by transposon mutagenesis of the M6 strain JRS4. This gene (srtA) encodes a protein homologous...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.8.2181-2191.2002
更新日期:2002-04-01 00:00:00