Abstract:
:Ribulose 1,5-bisphosphate carboxylase-oxygenase (RuBPC-O) activity was greatly enhanced when Rhodopseudomonas sphaeroides was grown in a mineral salts medium supplied with 1.5% CO2 in hydrogen. Analysis of cell extracts by sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that cells growing on 1.5% CO2 in H2 specifically accumulated RuBPC-O polypeptides. Quantitative immunological determinations revealed that accumulation of form I and form II RuBPC-O closely correlates with the increase of specific activity. However, the two enzymes appeared to be derepressed at different levels. Upon transfer from heterotrophic to autotrophic (1.5% CO2) growth conditions, the intracellular form I RuBPC-O concentration was augmented 17-fold, whereas the form II RuBPC-O content increased only fourfold. As a result, the form I-form II ratio changed from 0.5 to about 2.0. Since this change in the RuBPC-O ratio occurred in the early stage of growth, it suggests that form I RuBPC-O is required for growth under drastic CO2 limitation. The difference in the extent of derepression of form I and form II RuBPC-O also indicates that the synthesis of each enzyme is regulated somewhat independently of the other.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Jouanneau Y,Tabita FRdoi
10.1128/jb.165.2.620-624.1986subject
Has Abstractpub_date
1986-02-01 00:00:00pages
620-4issue
2eissn
0021-9193issn
1098-5530journal_volume
165pub_type
杂志文章abstract::Liquid holding recovery (LHR) in ultraviolet-irradiated Bacteroides fragilis cells occurred under aerobic conditions but was inhibited by anaerobic conditions. The increase in survival after aerobic LHR resulted in an increase in the shoulder regions of the ultraviolet survival curves. Maximum LHR was obtained after h...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.145.1.1-7.1981
更新日期:1981-01-01 00:00:00
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doi:10.1128/JB.105.3.1053-1062.1971
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pub_type: 杂志文章
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abstract::Cyclic adenosine 3',5'-monophosphate isolated from Mycobacterium smegmatis cells was identified by thin-layer chromatography, stepwise conversion to adenosine 5'-monophosphate and adenosine, ultraviolet absorption spectrum, phosphate analysis, and detection by two relatively specific radioisotopic methods. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.132.3.1031-1033.1977
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更新日期:2001-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.130.3.1024-1029.1977
更新日期:1977-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:1968-02-01 00:00:00
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更新日期:1969-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.2.697-703.1991
更新日期:1991-01-01 00:00:00
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doi:10.1128/JB.114.1.114-120.1973
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.11.6282-6290.1990
更新日期:1990-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2013-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1975-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2011-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.2.577-579.1967
更新日期:1967-02-01 00:00:00
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更新日期:1986-11-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.141.3.1454-1456.1980
更新日期:1980-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2008-09-01 00:00:00