Genetic loci responsible for incompatibility on a co-integrate plasmid, R100-1.

Abstract:

:An R plasmid, R100-1, was mapped previously (Yoshikawa, 1974) by transduction from an integratively suppressed Hfr strain to a recipient with a mutation in gene dnaA. By this method various types of transductants of plasmid R100-1 that exist autonomously or in the integrated state were obtained. Seventy-one such transductants were used in the present study to map gene inc, which is responsible for incompatibility. The results obtained can be explained by either of the following: (i) R100-1 has only a single gene or gene cluster (inc) despite previous work suggesting that this plasmid is a co-integrate of two replicons; (ii) R100-1 possesses more than one inc locus located between the repA and tra loci.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Yoshikawa M

doi

10.1128/JB.124.3.1097-1100.1975

keywords:

subject

Has Abstract

pub_date

1975-12-01 00:00:00

pages

1097-100

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

124

pub_type

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