Genetic variation of adenylation domains of the anabaenopeptin synthesis operon and evolution of substrate promiscuity.

abstract：:A lipopolysaccharide (LPS) has been isolated from the gram-negative photosynthetic bacterium Rhodopseudomonas capsulata. Chemical analysis revealed the presence of d-glucose, d-galactose, l-rhamnose, 3-O-methyl-l-rhamnose (l-acofriose), d-glucosamine, 2-keto-3-deoxyoctonate, and neuraminic acid. The LPS does not conta...

journal_title：Journal of bacteriology

authors： Weckesser J,Drews G,Fromme I

更新日期：1972-03-01 00:00:00

abstract：:Salmonella typhimurium contains three "major proteins" or "porins" (34K, 35K, and 36K) in the outer membrane. A mutant strain producing only the 35K porin was first grown in media containing high concentrations of NaCl to "repress" the porin synthesis and then was shifted into a medium without NaCl. The newly made por...

journal_title：Journal of bacteriology

authors： Smit J,Nikaido H

更新日期：1978-08-01 00:00:00

abstract：:The nucleotide sequence of the plasmid-encoded LlaKR2I restriction-modification (R-M) system of Lactococcus lactis subsp. lactis biovar diacetylactis KR2 was determined. This R-M system comprises divergently transcribed endonuclease (llaKR2IR) and methyltransferase (llaKR2IM) genes; located in the intergenic region is...

journal_title：Journal of bacteriology

authors： Twomey DP,McKay LL,O'Sullivan DJ

更新日期：1998-11-01 00:00:00

abstract：:The glnB gene from Bradyrhizobium japonicum, the endosymbiont of soybeans (Glycine max), was isolated and sequenced, and its expression was examined under various culture conditions and in soybean nodules. The B. japonicum glnB gene encodes a 12,237-dalton polypeptide that is highly homologous to the glnB gene product...

journal_title：Journal of bacteriology

authors： Martin GB,Thomashow MF,Chelm BK

更新日期：1989-10-01 00:00:00

abstract：UNLABELLED:Biofilm formation is responsible for increased antibiotic tolerance in pathogenic bacteria. Cyclic di-GMP (c-di-GMP) is a widely used second-messenger signal that plays a key role in bacterial biofilm formation. c-di-GMP is synthesized by diguanylate cyclases (DGCs), a conserved class of enzymes absent in ma...

journal_title：Journal of bacteriology

authors： Fernicola S,Paiardini A,Giardina G,Rampioni G,Leoni L,Cutruzzolà F,Rinaldo S

更新日期：2015-09-28 00:00:00

abstract：:We are engaged in the genetic and physical characterization of an enterotoxin (Ent) plasmid, Ent P307, which contains genes for the production of a hear-labile and a heat-stable enterotoxin. We are using an Escherichia coli K-12 strain, 711 (P307), constructed by S. Falkow, which contains no other plasmids besides Ent...

journal_title：Journal of bacteriology

authors： Santos DS,Palchaudhuri S,Maas WK

更新日期：1975-12-01 00:00:00

abstract：:Microorganisms and plants utilize two-component systems to regulate adaptive responses to changing environmental conditions. Sensor kinases detect stimuli and alter their autophosphorylation activity accordingly. Signal propagation occurs via the transfer of phosphoryl groups from upstream kinases to downstream respon...

journal_title：Journal of bacteriology

authors： Straughn PB,Vass LR,Yuan C,Kennedy EN,Foster CA,Bourret RB

更新日期：2020-07-09 00:00:00

abstract：:A nonswarming Serratia liquefaciens mutant deficient in serrawettin W2 production was constructed by transposon mutagenesis. Sequence homology indicated that insertion had occurred in gene swrA, which encodes a putative peptide synthetase. Expression of swrA is controlled by quorum sensing. ...

journal_title：Journal of bacteriology

authors： Lindum PW,Anthoni U,Christophersen C,Eberl L,Molin S,Givskov M

更新日期：1998-12-01 00:00:00

abstract：:The enzymes LinB(UT) and LinB(MI) (LinB from Sphingobium japonicum UT26 and Sphingobium sp. MI1205, respectively) catalyze the hydrolytic dechlorination of β-hexachlorocyclohexane (β-HCH) and yield different products, 2,3,4,5,6-pentachlorocyclohexanol (PCHL) and 2,3,5,6-tetrachlorocyclohexane-1,4-diol (TCDL), respecti...

journal_title：Journal of bacteriology

authors： Okai M,Ohtsuka J,Imai LF,Mase T,Moriuchi R,Tsuda M,Nagata K,Nagata Y,Tanokura M

更新日期：2013-06-01 00:00:00

abstract：:Estrada-Parra, Sergio (Escuela Nacional de Ciencias Biológicas, México, D.F., México), Abel Zamora, and L. F. Bojalil. Immunochemistry of the group-specific polysaccharide of Nocardia brasiliensis. J. Bacteriol. 90:571-574. 1965.-The group-specific polysaccharide of Nocardia brasiliensis was further purified, yielding...

journal_title：Journal of bacteriology

authors： Estrada-Parra S,Zamora A,Bojalil LF

更新日期：1965-09-01 00:00:00

abstract：:Competent cell-deoxyribonucleic acid (DNA) interactions were examined using tritium-labeled homologous or heterologous native or denatured DNAs and competent Streptococcus sanguis Wicky cells (strain WE4). The DNAs used were extracted from WE4 cells, Escherichia coli B cells, and E. coli bacteriophages T2, T4, T6, and...

journal_title：Journal of bacteriology

authors： Ranhand JM

更新日期：1980-05-01 00:00:00

abstract：:Fusidic acid or chloramphenicol was used to inhibit peptide synthesis to 1% of normal in Escherichia coli B, strain AS19. After 10 min of inhibition, peptide synthesis could be quickly restored to 80% of the normal rate after washing the bacteria on a filter. However, even in the presence of adenosine 3'-5'-cyclic-mon...

journal_title：Journal of bacteriology

authors： Pastushok C,Kennell D

更新日期：1974-02-01 00:00:00

abstract：:Expression levels of Bacillus amyloliquefaciens alpha-amylase, Escherichia coli TEM-beta-lactamase, and Semliki Forest virus glycoprotein E1 genes were compared in Bacillus subtilis. All three model genes were expressed by using a secretion vector, constructed by joining the B. amyloliquefaciens alpha-amylase promoter...

journal_title：Journal of bacteriology

authors： Ulmanen I,Lundström K,Lehtovaara P,Sarvas M,Ruohonen M,Palva I

更新日期：1985-04-01 00:00:00

abstract：:Phosphopantetheine adenylyltransferase (PPAT) from Escherichia coli is an essential hexameric enzyme that catalyzes the penultimate step in coenzyme A (CoA) biosynthesis and is a target for antibacterial drug discovery. The enzyme utilizes Mg-ATP and phosphopantetheine (PhP) to generate dephospho-CoA (dPCoA) and pyrop...

journal_title：Journal of bacteriology

authors： Miller JR,Ohren J,Sarver RW,Mueller WT,de Dreu P,Case H,Thanabal V

更新日期：2007-11-01 00:00:00

abstract：:The gene encoding the general stress transcription factor sigmaB in the gram-positive bacterium Listeria monocytogenes was isolated with degenerate PCR primers followed by inverse PCR amplification. Evidence for gene identification includes the following: (i) phylogenetic analyses of reported amino acid sequences for ...

journal_title：Journal of bacteriology

authors： Wiedmann M,Arvik TJ,Hurley RJ,Boor KJ

更新日期：1998-07-01 00:00:00

abstract：:Repaske, Roy (National Institute of Allergy and Infectious Diseases, Bethesda, Md.). Nutritional requirements for Hydrogenomonas eutropha. J. Bacteriol. 83: 418-422. 1962.-A simple apparatus for the autotrophic cultivation of Hydrogenomonas eutropha in 100-ml shake cultures is described. Nitrogen, in the form of ammon...

journal_title：Journal of bacteriology

authors： REPASKE R

更新日期：1962-02-01 00:00:00

abstract：:Youmans, Anne S. (Northwestern University Medical School, Chicago, Ill.) and Guy P. Youmans. Effect of mycosuppressin on the respiration and growth of Mycobacterium tuberculosis. J. Bacteriol. 84:708-715. 1962.-A substance, called mycosuppressin, was found in the lungs of guinea pigs and rabbits vaccinated with BCG or...

journal_title：Journal of bacteriology

authors： YOUMANS AS,YOUMANS GP

更新日期：1962-10-01 00:00:00

abstract：:A method was devised for the isolation of yeast monomorphic (LEV) mutants of Candida albicans. By this procedure, about 20 stable yeast-like mutants were isolated after mutagenesis with ethyl methane sulfonate. The growth rate of the mutants in different carbon sources, both fermentable and not, was indistinguishable ...

journal_title：Journal of bacteriology

authors： Elorza MV,Sentandreu R,Ruiz-Herrera J

更新日期：1994-04-01 00:00:00

abstract：:Elongation factor Tu (EF-Tu), encoded by tuf genes, carries aminoacyl-tRNA to the ribosome during protein synthesis. Duplicated tuf genes (tufA and tufB), which are commonly found in enterobacterial species, usually coevolve via gene conversion and are very similar to one another. However, sequence analysis of tuf gen...

journal_title：Journal of bacteriology

authors： Isabel S,Leblanc E,Boissinot M,Boudreau DK,Grondin M,Picard FJ,Martel EA,Parham NJ,Chain PS,Bader DE,Mulvey MR,Bryden L,Roy PH,Ouellette M,Bergeron MG

更新日期：2008-11-01 00:00:00

abstract：:Antipain kills Escherichia coli K-12 cells in an exponential manner beginning 1 h after its addition. Mutant strains, delta cya and crp, which are unable to synthesize cyclic adenosine 3',5'-monophosphate (cAMP) and the cAMP receptor protein, respectively, are not affected. Addition of cAMP (5 mM) to antipain-treated ...

journal_title：Journal of bacteriology

authors： Swenson PA

更新日期：1979-08-01 00:00:00

abstract：:Washed cell suspensions of Aquaspirillum magnetotacticum MS-1, A. itersonii E12639, Bacillus subtilis 6633, and Escherichia coli CSH27 translocated protons in response to the added oxidant O2 or NO3-, with triphenylmethylphosphonium bromide as the permeant ion. Iron respiration-driven proton translocation was observed...

journal_title：Journal of bacteriology

authors： Short KA,Blakemore RP

更新日期：1986-08-01 00:00:00

abstract：:Gram-negative bacteria acquire ferric siderophores through TonB-dependent outer membrane transporters (TBDT). By fluorescence spectroscopic hgh-throughput screening (FLHTS), we identified inhibitors of TonB-dependent ferric enterobactin (FeEnt) uptake through Escherichia coli FepA (EcoFepA). Among 165 inhibitors found...

journal_title：Journal of bacteriology

authors： Nairn BL,Eliasson OS,Hyder DR,Long NJ,Majumdar A,Chakravorty S,McDonald P,Roy A,Newton SM,Klebba PE

更新日期：2017-04-25 00:00:00

abstract：:Listeria monocytogenes is able to efficiently utilize glycerol as a carbon source. In a defined minimal medium, the growth rate (during balanced growth) in the presence of glycerol is similar to that in the presence of glucose or cellobiose. Comparative transcriptome analyses of L. monocytogenes showed high-level tran...

journal_title：Journal of bacteriology

authors： Joseph B,Mertins S,Stoll R,Schär J,Umesha KR,Luo Q,Müller-Altrock S,Goebel W

更新日期：2008-08-01 00:00:00

abstract：:Mycobacterium tuberculosis can persist for many years within host lung tissue without causing clinical disease. Little is known about the state in which the bacilli survive, although it is frequently referred to as dormancy. Some evidence suggests that cells survive in nutrient-deprived stationary phase. Therefore, we...

journal_title：Journal of bacteriology

authors： Smeulders MJ,Keer J,Speight RA,Williams HD

更新日期：1999-01-01 00:00:00

abstract：:Peptidoglycan hydrolase, LytF (CwlE), was determined to be identical to YhdD (deduced cell wall binding protein) by zymography after insertional inactivation of the yhdD gene. YhdD exhibits high sequence similarity with CwlF (PapQ, LytE) and p60 of Listeria monocytogenes. The N-terminal region of YhdD has a signal seq...

journal_title：Journal of bacteriology

authors： Ohnishi R,Ishikawa S,Sekiguchi J

更新日期：1999-05-01 00:00:00

abstract：:Growth rate control of adherent, sessile populations was achieved by the controlled perfusion of membrane-associated bacterial biofilms by the method of Gilbert et al. (P. Gilbert, D. G. Allison, D. J. Evans, P. S. Handley, and M. R. W. Brown, Appl. Environ. Microbiol. 55:1308-1311, 1989). Changes in cell surface hydr...

journal_title：Journal of bacteriology

authors： Allison DG,Evans DJ,Brown MR,Gilbert P

更新日期：1990-03-01 00:00:00

abstract：:Archaeal transcriptional machinery is similar to that of eukaryotes. We studied the fates of various components of the Sulfolobus solfataricus transcriptional apparatus under different stresses and found that in cells incubated at 90 degrees C for 1 h, transcription factor E (TFE) is selectively depleted, but its mRNA...

journal_title：Journal of bacteriology

authors： Iqbal J,Qureshi SA

更新日期：2010-06-01 00:00:00

abstract：:Many genomes of anaerobic hyperthermophiles encode multiple homologs of NAD(P)H oxidase that are thought to function in response to oxidative stress. We investigated one of the seven NAD(P)H oxidase homologs (TK1481) in the sulfur-reducing hyperthermophilic archaeon Thermococcus kodakarensis, focusing on the catalytic...

journal_title：Journal of bacteriology

authors： Kobori H,Ogino M,Orita I,Nakamura S,Imanaka T,Fukui T

更新日期：2010-10-01 00:00:00

abstract：:The reactive alpha-oxoaldehydes such as glyoxal (GO) and methylglyoxal (MG) are generated in vivo from sugars through oxidative stress. GO and MG are believed to be removed from cells by glutathione-dependent glyoxalases and other aldehyde reductases. We isolated a number of GO-resistant (GO(r)) mutants from Escherich...

journal_title：Journal of bacteriology

authors： Lee C,Kim I,Lee J,Lee KL,Min B,Park C

更新日期：2010-08-01 00:00:00

abstract：:Kasugamycin-resistant mutants of Bacillus subtilis were isolated and classified into two groups, one of which had resistance to kasugamycin in in vitro protein synthesis and mapped in the ribosomal region. The other group had no resistance to kasugamycin in in vitro protein synthesis and had weak cross-resistance to g...

journal_title：Journal of bacteriology

authors： Tominaga A,Kobayashi Y

更新日期：1978-09-01 00:00:00