Chemical analysis of and degradation studies on the cell wall lipopolysaccharide of Rhodopseudomonas capsulata.

Abstract:

:A lipopolysaccharide (LPS) has been isolated from the gram-negative photosynthetic bacterium Rhodopseudomonas capsulata. Chemical analysis revealed the presence of d-glucose, d-galactose, l-rhamnose, 3-O-methyl-l-rhamnose (l-acofriose), d-glucosamine, 2-keto-3-deoxyoctonate, and neuraminic acid. The LPS does not contain l-glycero-d-mannoheptose, a typical component of the LPS of enteric bacteria. Fatty acid analysis showed that, apart from lauric acid, two hydroxy fatty acids (hydroxycaproic and hydroxymyristic acids) are the main components. By hydrolysis in weak acid, the LPS has been separated into a polysaccharide part (degraded polysaccharide) and a lipid part (lipid A). Presumably the lipid A contains a glucosamine backbone. Whereas the OH-groups of glucosamine are esterified with lauric and hydroxycaproic acids, hydroxymyristic acid is linked to the amino group of the sugar. By separation of the degraded polysaccharide by gel filtration, a fraction has been isolated which inhibited hemagglutination in a system containing antiserum, obtained by immunization of rabbits with whole cells, and isolated LPS. This fraction, which includes the determinant group, contains the sugars glucose, rhamnose, and acofriose. A second fraction obtained in this way was found to be serologically inactive and is composed of glucose, galactose, neuraminic acid, and phosphate.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Weckesser J,Drews G,Fromme I

doi

10.1128/JB.109.3.1106-1113.1972

subject

Has Abstract

pub_date

1972-03-01 00:00:00

pages

1106-13

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

109

pub_type

杂志文章
  • The pneumococcal cell envelope stress-sensing system LiaFSR is activated by murein hydrolases and lipid II-interacting antibiotics.

    abstract::In the Firmicutes, two-component regulatory systems of the LiaSR type sense and orchestrate the response to various agents that perturb cell envelope functions, in particular lipid II cycle inhibitors. In the current study, we found that the corresponding system in Streptococcus pneumoniae displays similar properties ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01489-09

    authors: Eldholm V,Gutt B,Johnsborg O,Brückner R,Maurer P,Hakenbeck R,Mascher T,Håvarstein LS

    更新日期:2010-04-01 00:00:00

  • De novo genome project for the aromatic degrader Rhodococcus pyridinivorans strain AK37.

    abstract::Here, we present the complete genome sequence of Rhodococcus pyridinivorans AK37 strain NCAIM PB1376, which was isolated from an oil-polluted site in Hungary. R. pyridinivorans AK37 is an aerobic, nonsporulating, nonmotile, gram-positive bacterium with remarkable aromatic-decomposing activity. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06603-11

    authors: Kriszt B,Táncsics A,Cserháti M,Tóth Á,Nagy I,Horváth B,Nagy I,Tamura T,Kukolya J,Szoboszlay S

    更新日期:2012-03-01 00:00:00

  • Domains within RbpA Serve Specific Functional Roles That Regulate the Expression of Distinct Mycobacterial Gene Subsets.

    abstract::The RNA polymerase (RNAP) binding protein A (RbpA) contributes to the formation of stable RNAP-promoter open complexes (RPo) and is essential for viability in mycobacteria. Four domains have been identified in the RbpA protein, i.e., an N-terminal tail (NTT) that interacts with RNAP β' and σ subunits, a core domain (C...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00690-17

    authors: Prusa J,Jensen D,Santiago-Collazo G,Pope SS,Garner AL,Miller JJ,Ruiz Manzano A,Galburt EA,Stallings CL

    更新日期:2018-06-11 00:00:00

  • Protein phosphorylation in Bradyrhizobium japonicum bacteroids and cultures.

    abstract::Protein phosphorylation was demonstrated in Bradyrhizobium japonicum bacteroids in vivo and in cultures in vivo and in vitro. Comparison of in vivo-labeled phosphoproteins of bacteroids and of cultured cells showed differences in both the pattern and intensity of labeling. In cultured cells, comparison of the labeling...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.6.3420-3426.1989

    authors: Karr DB,Emerich DW

    更新日期:1989-06-01 00:00:00

  • Tumor induction by Agrobacterium tumefaciens: specific transfer of bacterial deoxyribonucleic acid to plant tissue.

    abstract::When Agrobacterium tumefaciens cells grown in the presence of tritiated thymidine to label specifically the bacterial deoxyribonucleic acid (DNA) are incubated with carrot root tissue for short periods of time, an appreciable fraction of the label becomes firmly associated with the root tissue. Such association is not...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.108.3.973-979.1971

    authors: Yajko DM,Hegeman GD

    更新日期:1971-12-01 00:00:00

  • Cytochemical localization of catalase activity in yeast peroxisomes.

    abstract::Diaminobenzidine oxidation product occurred in peroxisomes and in the intracristate spaces of mitochondria. The reaction was inhibited only in peroxisomes when 3-amino-1,2,4-triazole was present, but cyanide and azide inhibited deposition in both kinds of organelles. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.104.1.581-584.1970

    authors: Hoffmann HP,Szabo A,Avers CJ

    更新日期:1970-10-01 00:00:00

  • Carrier-mediated transport of folate in a mutant of Pediococcus cerevisiae.

    abstract::A mutant strain of Pediococcus cerevisiae (P. cerevisiae/PteGlu) was isolated which grows on low-folate (PteGlu) concentrations (200 pg/ml). The growth response of the parent and mutant strains to folinate (5-CHO-H(4)PteGlu) was the same. The transport of (14)C-PteGlu by P. cerevisiae/PteGlu was temperature-dependent ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.114.2.485-490.1973

    authors: Mandelbaum-Shavit F,Grossowicz N

    更新日期:1973-05-01 00:00:00

  • Preliminary survey of fungistatic properties of marine algae.

    abstract::Welch, Ann Marie (U. S. Veterans Administration Hospital, Durham, N. C.). Preliminary survey of fungistatic properties of marine algae. J. Bacteriol. 83:97-99. 1962-Homogenized preparations of 35 marine algae were tested for inhibitory activity against 6 pathogenic or opportunistically pathogenic fungi with saturated ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.1.97-99.1962

    authors: WELCH AM

    更新日期:1962-01-01 00:00:00

  • Hydrogen-dependent nitrogenase activity and ATP formation in Rhizobium japonicum bacteroids.

    abstract::Rhizobium japonicum 122 DES bacteroids from soybean nodules possess an active H(2)-oxidizing system that recycles all of the H(2) lost through nitrogenase-dependent H(2) evolution. The addition of 72 muM H(2) to suspensions of bacteroids increased O(2) uptake 300% and the rate of C(2)H(2) reduction 300 to 500%. The op...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.137.1.153-160.1979

    authors: Emerich DW,Ruiz-Argüeso T,Ching TM,Evans HJ

    更新日期:1979-01-01 00:00:00

  • Properties of pGC1, a lac plasmid orginating in Yersinia enterocolitica 842.

    abstract::pGC1, a self-transmissible lac plasmid that originated in Yersinia enterocolitica 842, is described. This plasmid is freely transmissible between strains of Y. enterocolitica and from such strains to strains of Escherichia coli. The plasmid can also be transferref among G. coli strains and back to Y. enterocolitica, a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.127.3.1058-1062.1976

    authors: Cornelis G,Bennett PM,Grinsted J

    更新日期:1976-09-01 00:00:00

  • Cloning, sequencing, expression, and complementation analysis of the Escherichia coli K1 kps region 1 gene, kpsE, and identification of an upstream open reading frame encoding a protein with homology to GutQ.

    abstract::The kps locus for polysialic acid capsule expression in Escherichia coli K1 is composed of a central group of biosynthetic neu genes, designated region 2, flanked on either side by region 1 or region 3 kps genes with poorly defined functions. Chromosomal mutagenesis with MudJ and subsequent complementation analysis, m...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.24.8018-8023.1993

    authors: Cieslewicz MJ,Steenbergen SM,Vimr ER

    更新日期:1993-12-01 00:00:00

  • Effects of oxygen and heme on the development of a microbial respiratory system.

    abstract::The effect of adding hemin to anaerobically grown cells of a strain of Staphylococcus epidermidis, which was heme-deficient due to anaerobic growth, has been examined. Cells grown anaerobically in media containing hemin exhibited a marked increase in several oxidative activities as compared with cells grown anaerobica...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.93.1.278-285.1967

    authors: Jacobs NJ,Maclosky ER,Conti SF

    更新日期:1967-01-01 00:00:00

  • Purification, characterization, and sequence analysis of 2-aminomuconic 6-semialdehyde dehydrogenase from Pseudomonas pseudoalcaligenes JS45.

    abstract::2-Aminonumconic 6-semialdehyde is an unstable intermediate in the biodegradation of nitrobenzene and 2-aminophenol by Pseudomonas pseudoalcaligenes JS45. Previous work has shown that enzymes in cell extracts convert 2-aminophenol to 2-aminomuconate in the presence of NAD+. In the present work, 2-aminomuconic semialdeh...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.17.4591-4595.1998

    authors: He Z,Davis JK,Spain JC

    更新日期:1998-09-01 00:00:00

  • Visualization of Pseudomonas aeruginosa O antigens by using a protein A-dextran-colloidal gold conjugate with both immunoglobulin G and immunoglobulin M monoclonal antibodies.

    abstract::Two lipopolysaccharide O-antigen-specific monoclonal antibodies, MA1-8 (an immunoglobulin G1 [IgG1]) and MF15-4 (an IgM), were used to localize the O antigen of the lipopolysaccharide of Pseudomonas aeruginosa PAO1. A protein A-dextran-gold conjugate with an average particle diameter of 12.5 nm was used to label bacte...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.8.3531-3538.1987

    authors: Lam JS,Lam MY,MacDonald LA,Hancock RE

    更新日期:1987-08-01 00:00:00

  • Transcriptional analysis and mutation of a dnaA-like gene in Synechocystis sp. strain PCC 6803.

    abstract::Transcription of the dnaA gene of the cyanobacterium Synechocystis sp. strain PCC 6803 is light dependent and yields a monocistronic mRNA, as determined by Northern analysis. Surprisingly, mutants with inactivated dnaA were viable. In batch cultures under standard conditions, the mutants grew like the wild type and di...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.18.4946-4949.1998

    authors: Richter S,Hagemann M,Messer W

    更新日期:1998-09-01 00:00:00

  • Enzymatic and non-enzymatic reduction of nitrite by extracts of Neurospora crassa.

    abstract::Two activites causing nitrite disappearance are found in extracts of Neurospora; one, inducible by nitrate or nitrite and present only in nitrite-utilizing strains, catalyze the stoichiometric reduction of nitrite to ammonia; the other, present in all strains under all conditions, causes the disappearance of nitrite t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.123.2.755-758.1975

    authors: Chang HC,Mulkins GJ,Dyer JC,Sorger GJ

    更新日期:1975-08-01 00:00:00

  • The VirA protein of Agrobacterium tumefaciens is autophosphorylated and is essential for vir gene regulation.

    abstract::The virA and virG gene products are required for the regulation of the vir regulon on the tumor-inducing (Ti) plasmid of Agrobacterium tumefaciens. VirA is a membrane-associated protein which is homologous to the sensor molecules of other two-component regulatory systems. We overproduced truncated VirA proteins in Esc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.2.525-530.1990

    authors: Jin S,Roitsch T,Ankenbauer RG,Gordon MP,Nester EW

    更新日期:1990-02-01 00:00:00

  • Localization of the Escherichia coli rnt gene encoding RNase T by using a combination of physical and genetic mapping.

    abstract::The rnt gene encoding RNase T was cloned on a 13-kilobase BamHI fragment. Restriction analysis of the fragment and comparison of it with the Escherichia coli restriction map localized rnt to kilobase coordinates 1733 to 1746, corresponding to about 36 min on the genetic map. The map location was confirmed by cotransdu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.10.5736-5737.1989

    authors: Case LM,Chen XN,Deutscher MP

    更新日期:1989-10-01 00:00:00

  • Transcriptional Landscape and Regulatory Roles of Small Noncoding RNAs in the Oxidative Stress Response of the Haloarchaeon Haloferax volcanii.

    abstract::Haloarchaea in their natural environment are exposed to hypersalinity, intense solar radiation, and desiccation, all of which generate high levels of oxidative stress. Previous work has shown that haloarchaea are an order of magnitude more resistant to oxidative stress than most mesophilic organisms. Despite this resi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00779-17

    authors: Gelsinger DR,DiRuggiero J

    更新日期:2018-04-09 00:00:00

  • Difference between manganese ion requirements of pediococci and enterococci.

    abstract::For maximal turbidity and dissimilatory activity, three strains of pediococci required the addition of Mn(2+) to Rogosa basal medium. Twenty-two strains of enterococci proved indifferent to this supplement. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.112.1.627-628.1972

    authors: Efthymiou CJ,Joseph SW

    更新日期:1972-10-01 00:00:00

  • Purification and properties of undecyl acetate esterase from Pseudomonas cepacia grown on 2-tridecanone.

    abstract::Undecyl acetate esterase has been purified from Pseudomonas cepacia grown on the methyl ketone, 2-tridecanone. The K(m) for undecyl acetate was 2.3 x 10(-2) M. Polyacrylamide gel electrophoresis indicated that two esterase bands were being recovered during purification. These bands were separated by preparative polyac...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.118.3.880-889.1974

    authors: Shum AC,Markovetz AJ

    更新日期:1974-06-01 00:00:00

  • A Caulobacter gene involved in polar morphogenesis.

    abstract::At specific times in the cell cycle, the bacterium Caulobacter crescentus assembles two major polar organelles, the flagellum and the stalk. Previous studies have shown that flbT mutants overproduce flagellins and are unable to form chemotaxis swarm rings. In this paper, we report alterations in both the stalk and the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.4.2113-2123.1990

    authors: Driks A,Schoenlein PV,DeRosier DJ,Shapiro L,Ely B

    更新日期:1990-04-01 00:00:00

  • Effect of specific immune mouse serum on the growth of Salmonella enteritidis in nonvaccinated mice challenged by various routes.

    abstract::Salmonella enteritidis was injected intravenously, intraperitoneally, or subcutaneously into specific pathogen-free mice. The number of organisms in the blood, liver, spleen, peritoneal cavity, and draining inguinal lymph node was determined by daily enumeration. Opsonization of the organism with hyperimmune serum inc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.97.2.667-675.1969

    authors: Collins FM

    更新日期:1969-02-01 00:00:00

  • Point mutations in a conserved region (TonB box) of Escherichia coli outer membrane protein BtuB affect vitamin B12 transport.

    abstract::Uptake of cobalamins and iron chelates in Escherichia coli K-12 is dependent on specific outer membrane transport proteins and the energy-coupling function provided by the TonB protein. The btuB product is the outer membrane receptor for cobalamins, bacteriophage BF23, and the E colicins. A short sequence near the ami...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.12.6526-6533.1989

    authors: Gudmundsdottir A,Bell PE,Lundrigan MD,Bradbeer C,Kadner RJ

    更新日期:1989-12-01 00:00:00

  • Methods for the determination of deoxyribonucleic Acid homologies in streptomyces.

    abstract::Variations of the membrane filter technique for deoxyribonucleic acid (DNA) hybridizations were studied with respect to Streptomyces species. At the temperatures required for specific hybridization of DNA with the high melting temperature (T(m)) characteristic of Streptomyces, large amounts (up to 97%) of filter-bound...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.104.3.1086-1094.1970

    authors: Okanishi M,Gregory KF

    更新日期:1970-12-01 00:00:00

  • In vivo definition of an archaeal promoter.

    abstract::We have used a plasmid-based transcriptional reporter system to examine the transcriptional effects of 33 single point mutations in the box A region (TATA-like sequence) of the Haloferax volcanii tRNA(Lys) promoter. The most pronounced effects on transcriptional efficiency were found when the nucleotides corresponding...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.7.1844-1849.1995

    authors: Palmer JR,Daniels CJ

    更新日期:1995-04-01 00:00:00

  • Cloning and characterization of the gene for a protein thiol-disulfide oxidoreductase in Bacillus brevis.

    abstract::The gene (bdb) for protein thiol-disulfide oxidoreductase cloned from Bacillus brevis was found to encode a polypeptide consisting of 117 amino acid residues with a signal peptide of 27 residues. Bdb contains a well-conserved motif, Cys-X-X-Cys, which functions as the active center of disulfide oxidoreductases such as...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.3.745-749.1995

    authors: Ishihara T,Tomita H,Hasegawa Y,Tsukagoshi N,Yamagata H,Udaka S

    更新日期:1995-02-01 00:00:00

  • Oxygen-mediated regulation of porphobilinogen formation in Rhodobacter capsulatus.

    abstract::A Rhodobacter capsulatus hemC mutant has been isolated and used to show that oxygen regulates the intracellular levels of porphobilinogen. Experiments using a hemB-cat gene fusion demonstrated that oxygen does not transcriptionally regulate hemB transcription. Porphobilinogen synthase activity is not regulated by oxyg...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.6.1685-1692.2002

    authors: Biel AJ,Canada K,Huang D,Indest K,Sullivan K

    更新日期:2002-03-01 00:00:00

  • Evidence for adenylate nucleotide transport (ATP-ADP translocation) in vesicles of Frankia sp. strain EAN1pec.

    abstract::Atractyloside and carboxyatractyloside partially inhibited nitrogenase activity (acetylene reduction) by isolated vesicles of Frankia strain EAN1pec. Extracts of disrupted vesicles showed nitrogenase activity that was not affected by the inhibitors. The vesicles accumulated ATP by an atractyloside-sensitive mechanism....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.7.3053-3057.1988

    authors: Tisa LS,Ensign JC

    更新日期:1988-07-01 00:00:00

  • A Ti plasmid-encoded enzyme required for degradation of mannopine is functionally homologous to the T-region-encoded enzyme required for synthesis of this opine in crown gall tumors.

    abstract::The mocC gene encoded by the octopine/mannityl opine-type Ti plasmid pTi15955 is related at the nucleotide sequence level to mas1' encoded by the T region of this plasmid. While Mas1 is required for the synthesis of mannopine (MOP) by crown gall tumor cells, MocC is essential for the utilization of MOP by Agrobacteriu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.11.3285-3292.1996

    authors: Kim KS,Chilton WS,Farrand SK

    更新日期:1996-06-01 00:00:00