Abstract:
:We have used a plasmid-based transcriptional reporter system to examine the transcriptional effects of 33 single point mutations in the box A region (TATA-like sequence) of the Haloferax volcanii tRNA(Lys) promoter. The most pronounced effects on transcriptional efficiency were found when the nucleotides corresponding to the TATA-like region were altered. Promoters with wild-type or higher levels of transcriptional activity conformed to the general archaeal box A consensus, 5'-T/CTTAT/AA-3'. The preference for a pyrimidine residue in the 5' position of this region and the exclusion of guanine and cytosine in the next four positions in the 3' direction are defining characteristics shared by all efficient archaeal promoters. We have also observed that replacement of a 10-nucleotide purine-rich sequence, located 5' of the H. volcanii tRNA(Lys) box A element, completely abolished transcription from this promoter. These data show that the H. volcanii tRNA(Lys) promoter is dependent on two separate, and essential, sequence elements. The possible functions of these sequences, in view of the recent descriptions of eucaryal-like transcription factors for Archaea, are discussed.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Palmer JR,Daniels CJdoi
10.1128/jb.177.7.1844-1849.1995subject
Has Abstractpub_date
1995-04-01 00:00:00pages
1844-9issue
7eissn
0021-9193issn
1098-5530journal_volume
177pub_type
杂志文章abstract::Escherichia coli contains 30 two-component systems (TCSs), each consisting of a histidine kinase and a response regulator. Whereas most TCSs are well characterized in this model organism, little is known about the YpdA/YpdB system. To identify YpdB-regulated genes, we compared the transcriptomes of E. coli cells overp...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02051-12
更新日期:2013-02-01 00:00:00
abstract::Cultural characteristics and serological relationships of pathogenic marine vibrios isolated from fish in the Pacific Northwest were studied. These organisms were compared with cultures of Vibrio anguillarum, a known fish pathogen. On the basis of morphological and cultural characteristics, the Pacific Northwest strai...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.3.1242-1247.1969
更新日期:1969-12-01 00:00:00
abstract::The effect of dihydrostreptomycin on the incorporation of amino acids into protein in antibiotic-deprived cells of a streptomycin-dependent strain of Escherichia coli B has been compared with its effect on protein synthesis in extracts from cells of the same strain. Stimulation of phenylalanine incorporation into prot...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.1.247-253.1969
更新日期:1969-10-01 00:00:00
abstract::MurG is a peripheral membrane protein that is one of the key enzymes in peptidoglycan biosynthesis. The crystal structure of Escherichia coli MurG (S. Ha, D. Walker, Y. Shi, and S. Walker, Protein Sci. 9:1045-1052, 2000) contains a hydrophobic patch surrounded by basic residues that may represent a membrane associatio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.13.3773-3779.2003
更新日期:2003-07-01 00:00:00
abstract::Escherichia coli suffered 95 to 100% lethality when exposed to 1.0 mM paraquat for 30 min at 37 degrees C in aerobic nutrient broth medium but did not lose viability when the exposure was done in Vogel Bonner or tryptic soy yeast extract medium. Paraquat was, however, bacteriostatic in all of these media. Salts, added...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.167.1.346-349.1986
更新日期:1986-07-01 00:00:00
abstract::Seligman, Stephen J. (University of California, Los Angeles) and William L. Hewitt. Kinetics of the action of ampicillin on Escherichia coli. J. Bacteriol. 85:1160-1164. 1963.-The curve of the number of viable Escherichia coli after exposure to ampicillin can be divided into three phases: a lag phase, a rapid bacteric...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.85.5.1160-1164.1963
更新日期:1963-05-01 00:00:00
abstract::Upon incubation of Agrobacterium tumefaciens A348 with acetosyringone, the vir genes encoded by the Ti (tumor-inducing) plasmid are induced. The addition of certain opines, including octopine, nopaline, leucinopine, and succinamopine, enhanced this induction 2- to 10-fold. The compounds mannopine, acetopine, arginine,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.7.3696-3703.1989
更新日期:1989-07-01 00:00:00
abstract::Mycoplasma penetrans is a newly identified species of the genus MYCOPLASMA: It was first isolated from a urine sample from a human immunodeficiency virus (HIV)-infected patient. M. penetrans changes its surface antigen profile with high frequency. The changes originate from ON<==>OFF phase variations of the P35 family...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.1.231-242.2003
更新日期:2003-01-01 00:00:00
abstract::One of the pleiotropic phenotypes of mutations affecting DNA gyrase activity in Salmonella typhimurium is the constitutive deattenuation of the histidine operon. In the present work, we isolated and characterized a suppressor mutation which restores his attenuation in the presence of a defective gyrase. Such a suppres...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.8.2216-2226.1994
更新日期:1994-04-01 00:00:00
abstract::Korormicin is an antibiotic produced by some pseudoalteromonads which selectively kills Gram-negative bacteria that express the Na+-pumping NADH:quinone oxidoreductase (Na+-NQR.) We show that although korormicin is an inhibitor of Na+-NQR, the antibiotic action is not a direct result of inhibiting enzyme activity. Ins...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00718-18
更新日期:2019-05-08 00:00:00
abstract::The lemA gene of Pseudomonas syringae pv. syringae encodes the sensor kinase of a bacterial two-component signal transduction system. Phenotypes that are lemA dependent in P. syringae include lesion formation on bean and production of extracellular protease and the antibiotic syringomycin. Recently, the gacA gene has ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.6.1548-1555.1996
更新日期:1996-03-01 00:00:00
abstract::Phosphotransacetylase and acetate kinase catalyze the activation of acetate to acetyl coenzyme A in the first step of methanogenesis from acetate in Methanosarcina thermophila. The genes encoding these enzymes (pta and ack) have been cloned and sequenced. They are arranged on the chromosome with pta upstream of ack (M...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.7.1699-1702.1995
更新日期:1995-04-01 00:00:00
abstract::We isolated an Escherichia coli mutant strain that suppresses the glycolate-negative phenotype of a strain deficient in both GlcA and LldP transporters of this compound. This suppressing phenotype was assigned to yjcG, a gene whose function was previously unknown, which was found to encode a membrane protein able to t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.21.6448-6455.2003
更新日期:2003-11-01 00:00:00
abstract::The AraC homolog ToxT coordinately regulates virulence gene expression in Vibrio cholerae. ToxT is required for transcriptional activation of the genes encoding cholera toxin and the toxin coregulated pilus, among others. In this work we focused on the interaction of ToxT with the tcpA promoter and investigated the me...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.20.5533-5544.2002
更新日期:2002-10-01 00:00:00
abstract::Deletion of bacterial DNA fragments is stimulated in induced Mucts62 lysogens. The host genes located proximally to the prophage are more frequently lost than those which are unlinked to the Mu genome. Genes located on either side of a Mu genome are deleted in the same manner. Like the other Mu-induced rearrangements,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.136.2.477-483.1978
更新日期:1978-11-01 00:00:00
abstract::Copper is both a required micronutrient and a source of toxicity in most organisms, including Campylobacter jejuni Two proteins expressed in C. jejuni (termed CopA and CueO) have been shown to be a copper transporter and multicopper oxidase, respectively. We have isolated strains with mutations in these genes, and her...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00208-18
更新日期:2018-10-10 00:00:00
abstract::Complementation analyses using minimal recombinant clones showed that all known pdx point mutations, which cause pyridoxine (vitamin B6) or pyridoxal auxotrophy, are located in the pdxA, pdxB, serC, pdxJ, and pdxH genes. Antibiotic enrichments for chromosomal transposon mutants that require pyridoxine (vitamin B6) or ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.5.1554-1567.1992
更新日期:1992-03-01 00:00:00
abstract::Mutants of Escherichia coli K-12 unable to excise pyrimidine dimers from their deoxyribonucleic acid (DNA) because of a uvr mutation show a higher survival when plated on a minimal salts medium after exposure to ultraviolet radiation than when plated on a complex medium such as nutrient agar containing yeast extract. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.102.2.404-410.1970
更新日期:1970-05-01 00:00:00
abstract::The replication order of markers was studied in Bacillus subtilis strains bearing the trpE26 mutation by the use of the density transfer technique. An important difference in this order was observed in comparison with that of strain 168 T-. All markers tested of a chromosome segment extending from trpD to ilvA replica...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.122.3.886-898.1975
更新日期:1975-06-01 00:00:00
abstract::Sortases are transamidases that covalently link proteins to the peptidoglycan of gram-positive bacteria. The genome of the pathogenic bacterium Listeria monocytogenes encodes two sortases genes, srtA and srtB. The srtA gene product anchors internalin and some other LPXTG-containing proteins to the listerial surface. H...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.7.1972-1982.2004
更新日期:2004-04-01 00:00:00
abstract::Previously we have described a mutant Hfr strain in which incompatibility between the integrated F factor and an autonomous F-prime (F') factor was abolished. The mutation (inc) was located in the integrated F factor. F-prime factors isolated from the mutant Hfr strain have the same incompatibility behavior as those i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.127.1.348-353.1976
更新日期:1976-07-01 00:00:00
abstract::Slein, Milton W. (Fort Detrick, Frederick, Md.) and Gerald F. Logan, Jr. Mechanism of action of the toxin of Bacillus anthracis. II. Alkaline phosphatasemia produced by culture filtrates of various bacilli. J. Bacteriol. 83:359-369. 1962.-A factor which produces hyperphosphatasemia after intravenous injection into ani...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.2.359-369.1962
更新日期:1962-02-01 00:00:00
abstract::The activity of the ATP:GTP 3'-pyrophosphotransferase (guanosine pentaphosphate synthetase I [GPSI]) from Streptomyces antibioticus is stimulated maximally by methanol at 20% (vol/vol) in assay mixtures. Although the enzyme is not activated by ribosomes, its activity is stimulated by tRNA (uncharged or charged) and by...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.5.1482-1487.1994
更新日期:1994-03-01 00:00:00
abstract::An obligate thermophilic strain of Bacillus stearothermophilus, strain PH24, isolated from industrial sediment by elective culture, grew readily at 55 C on phenol or on one of the isomers of cresol as the major carbon source. Intact cells grown in the presence of phenol, o-cresol, m-cresol, or p-cresol were induced to...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.124.3.1077-1083.1975
更新日期:1975-12-01 00:00:00
abstract::The major outer membrane protein of molecular weight 40,000 (the 40K protein) of a virulent isolate of Bordetella pertussis was purified to apparent homogeneity. The purified protein formed an oligomer band (of apparent molecular weight 90,000) on sodium dodecyl sulfate-polyacrylamide gels after solubilization at low ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.166.1.212-216.1986
更新日期:1986-04-01 00:00:00
abstract::Production of Bacillus subtilis exoproteases is positively regulated by the DegS-DegU two-component regulatory system and other regulatory factors including DegR and ProB. It was shown that the expression of degR was virtually abolished in a sigD mutant and that the transcriptional initiation site in vivo is preceded ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.1.216-222.1996
更新日期:1996-01-01 00:00:00
abstract::An apparent operator-constitutive mutation was discovered in the repressible acid phosphatase system in Saccharomyces cerevisiae. The site of mutation, designated PHOO, was found to be closely linked to the phoD locus. The mutant allele, PHOO, was semidominant over the wild-type allele and effective for the expression...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.2.608-617.1974
更新日期:1974-11-01 00:00:00
abstract::Four genes, dra, tpp, drm, and pup, that specify enzymes involved in the catabolism of nucleosides and deoxynucleosides in Escherichia coli are known to be very closely linked in the order dra-tpp-drm-pup. By infecting cells with the phage Mu-1 and isolating low-thymine-requiring derivatives of a strain lacking thymid...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.121.2.475-484.1975
更新日期:1975-02-01 00:00:00
abstract::Pleiotropic effects of two ribosomal mutations for cycloheximide resistance were studied in a double-resistant homocaryon. The results obtained indicated that the combination of the two ribosomal mutations results in: (i) morphological abnormalities which suggest a severe distortion in the extension of cell walls and ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.2.620-624.1979
更新日期:1979-08-01 00:00:00
abstract::Yersinia pestis is an important human pathogen that is maintained in flea-rodent enzootic cycles in many parts of the world. During its life cycle, Y. pestis senses host-specific environmental cues such as temperature and regulates gene expression appropriately to adapt to the insect or mammalian host. For example, Y....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.4.1381-1388.2006
更新日期:2006-02-01 00:00:00