Stimulation of deletions in the Escherichia coli chromosome by partially induced Mucts62 prophages.

abstract：:Quantitative mass conversion of intact bacterial cells of Bacillus subtilis and B. licheniformis to L-phase variants has been effected after lysozyme treatment. After subculture of the unstable L-phase variants for several months in the presence of methicillin, stable L-phase variants were obtained which grew in the a...

journal_title：Journal of bacteriology

authors： Wyrick PB,Rogers HJ

更新日期：1973-10-01 00:00:00

abstract：:Signal transduction protein P(II) is dephosphorylated in Synechocystis sp. strain PCC 6803 by protein phosphatase PphA. To determine the impact of PphA-mediated P(II) dephosphorylation on physiology, the phenotype of a PphA-deficient mutant was analyzed. Mutants lacking either PphA or P(II) were impaired in efficient ...

journal_title：Journal of bacteriology

authors： Kloft N,Forchhammer K

更新日期：2005-10-01 00:00:00

abstract：:Toluene dioxygenase from Pseudomonas putida F1 has been studied extensively with aromatic substrates. The present work examined the toluene dioxygenase-catalyzed oxidation of various halogenated ethenes, propenes, butenes and nonhalogenated cis-2-pentene, an isomeric mix of 2-hexenes, cis-2-heptene, and cis-2-octene a...

journal_title：Journal of bacteriology

authors： Lange CC,Wackett LP

更新日期：1997-06-01 00:00:00

abstract：:The surface structure and cell envelope layers of various virulent Bacteroides nodosus strains were examined by light microscopy and by electron microscopy by using negative staining, thin-section, and freeze-fracture-etch techniques. Three surface structures were described: pili and a diffuse material, both of which ...

journal_title：Journal of bacteriology

authors： Every D,Skerman TM

更新日期：1980-02-01 00:00:00

abstract：:SigB is an alternative sigma factor that controls a large regulon in Staphylococcus aureus. Activation of SigB requires RsbU, a protein phosphatase 2C (PP2C)-type phosphatase. In a closely related organism, Bacillus subtilis, RsbU activity is stimulated upon interaction with RsbT, a kinase, which following an activati...

journal_title：Journal of bacteriology

authors： Pané-Farré J,Jonas B,Hardwick SW,Gronau K,Lewis RJ,Hecker M,Engelmann S

更新日期：2009-04-01 00:00:00

abstract：:The gene for a new outer membrane-associated protease, designated OmpP, of Escherichia coli has been cloned and sequenced. The gene encodes a 315-residue precursor protein possessing a 23-residue signal sequence. Including conservative substitutions and omitting the signal peptides, OmpP is 87% identical to the outer ...

journal_title：Journal of bacteriology

authors： Kaufmann A,Stierhof YD,Henning U

更新日期：1994-01-01 00:00:00

abstract：:Members of the Streptococcus bovis group are important causes of endocarditis. However, factors associated with their pathogenicity, such as adhesins, remain uncharacterized. We recently demonstrated that endocarditis-derived Streptococcus gallolyticus subsp. gallolyticus isolates frequently adhere to extracellular ma...

journal_title：Journal of bacteriology

authors： Sillanpää J,Nallapareddy SR,Qin X,Singh KV,Muzny DM,Kovar CL,Nazareth LV,Gibbs RA,Ferraro MJ,Steckelberg JM,Weinstock GM,Murray BE

更新日期：2009-11-01 00:00:00

abstract：:The fim operon of Salmonella enterica serovar Typhimurium encodes type 1 fimbriae. The expression of fim is controlled in response to environmental signals through a complex regulatory cascade involving the proteins FimW, FimY, and FimZ and a genetic locus, fimU, that encodes a rare arginine tRNA. We discovered that a...

journal_title：Journal of bacteriology

authors： McFarland KA,Lucchini S,Hinton JC,Dorman CJ

更新日期：2008-01-01 00:00:00

abstract：:In the course of study on ribonucleic acid (RNA) metabolism during sporulation in Saccharomyces cerevisiae, a new species of RNA (20S) was observed in sporulating cells by polyacrylamide gel electrophoresis. The relative content of this RNA to total RNA increased linearly early in sporulation. Labeled adenine was pref...

journal_title：Journal of bacteriology

authors： Kadowaki K,Halvorson HO

更新日期：1971-03-01 00:00:00

abstract：:A total of 508 random clones from five Mycoplasma genitalium genomic libraries were partially sequenced and analyzed. This resulted in the identification of 291 unique contigs. Sequence information from these clones (100,993 nucleotides), representing approximately 17% of this pathogen's genome, was analyzed by compar...

journal_title：Journal of bacteriology

authors： Peterson SN,Hu PC,Bott KF,Hutchison CA 3rd

更新日期：1993-12-01 00:00:00

abstract：:CRM 228 (T. Uchida, A. M. Pappenheimer, and R. Greany, J. Biol. Chem. 248:3838-3844, 1973), a mutant form of diphtheria toxin which completely lacks ADP-ribosyltransferase activity, contains five amino acid substitutions. The two amino acid changes that fall within the A chain of the toxin (G79D and E162K) were separa...

journal_title：Journal of bacteriology

authors： Johnson VG,Nicholls PJ

更新日期：1994-08-01 00:00:00

abstract：:Massetolide A is a cyclic lipopeptide (CLP) antibiotic produced by various Pseudomonas strains from diverse environments. Cloning, sequencing, site-directed mutagenesis, and complementation showed that massetolide A biosynthesis in P. fluorescens SS101 is governed by three nonribosomal peptide synthetase (NRPS) genes,...

journal_title：Journal of bacteriology

authors： de Bruijn I,de Kock MJ,de Waard P,van Beek TA,Raaijmakers JM

更新日期：2008-04-01 00:00:00

abstract：:Since relatively few quantitative immunological studies had been conducted with invertebrates, this study was designed to learn more about the immune potential of the oyster, a molluscan representative. Experiments measured the primary and secondary clearance rates of T2 coliphage in the oyster. The fate of phage inje...

journal_title：Journal of bacteriology

authors： Acton RT,Evans EE

更新日期：1968-04-01 00:00:00

abstract：:Mitochondrial protein synthesis was studied during release from glucose repression in Saccharomyces cerevisiae cells bearing different mitochondrial genomes. The increase in the rate of the synthesis of mitochondrial translation products was analyzed during respiratory induction. Different kinetic patterns were found ...

journal_title：Journal of bacteriology

authors： Falcone C,Agostinelli M,Frontali L

更新日期：1983-03-01 00:00:00

abstract：:The N-terminal amino acid sequence of the polyhydroxyalkanoic acid (PHA) granule-associated M(r)-15,500 protein of Rhodococcus ruber (the GA14 protein) was analyzed. The sequence revealed that the corresponding structural gene is represented by open reading frame 3, encoding a protein with a calculated M(r) of 14,175 ...

journal_title：Journal of bacteriology

authors： Pieper-Fürst U,Madkour MH,Mayer F,Steinbüchel A

更新日期：1994-07-01 00:00:00

abstract：:Shigella spp. are the major cause of bacillary dysentery worldwide. The pathogenic process involves bacterial invasion and lysis of the phagocytic vacuole, followed by replication and movement within the cell cytoplasm and, ultimately, spread directly into adjacent cells. This study demonstrates that S. flexneri cytoc...

journal_title：Journal of bacteriology

authors： Way SS,Sallustio S,Magliozzo RS,Goldberg MB

更新日期：1999-02-01 00:00:00

abstract：:Mutants for 9 of the 10 steps in histidine biosynthesis have been isolated and identified by enzyme assay. Each locus has been mapped in relation to the aro cluster and to other histidine loci by deoxyribonucleic acid-mediated transformation. The genes which code for enzymes 3, 6, and 8 of the pathway are linked to th...

journal_title：Journal of bacteriology

authors： Chapman LF,Nester EW

更新日期：1969-03-01 00:00:00

abstract：:Caldwell, D. R. (U.S. Department of Agriculture, Beltsville, Md.), D. C. White, M. P. Bryant, and R. N. Doetsch. Specificity of the heme requirement for growth of Bacteroides ruminicola. J. Bacteriol. 90:1645-1654. 1965.-Previous studies suggested that most strains of Bacteroides ruminicola subsp. ruminicola require h...

journal_title：Journal of bacteriology

authors： Caldwell DR,White DC,Bryant MP,Doetsch RN

更新日期：1965-12-01 00:00:00

abstract：:Rhizobium japonicum, capable of binding high-molecular-weight donor (32)P-labeled deoxyribonucleic acid (DNA) during late log phase in a competence medium, was transformed for streptomycin resistance with a frequency of transformation ranging between 0.02 and 0.08%. Eight to 10% of the homologous native (32)P-labeled ...

journal_title：Journal of bacteriology

authors： Raina JL,Modi VV

更新日期：1972-08-01 00:00:00

abstract：:DNA from the STA1 (extracellular glucoamylase) gene of Saccharomyces diastaticus was used as a probe to enable the cloning by colony hybridization of three DNA fragments from Saccharomyces cerevisiae; these were designated S1, S2, and SGA (intracellular, sporulation-specific glucoamylase gene). To examine the evolutio...

journal_title：Journal of bacteriology

authors： Yamashita I,Nakamura M,Fukui S

更新日期：1987-05-01 00:00:00

abstract：:The amounts of glycogen and trehalose have been measured in cells of a prototrophic diploid yeast strain subjected to a variety of nutrient limitations. Both glycogen and trehalose were accumulated in cells deprived specifically of nirogen, sulfur, or phosphorus, suggesting that reserve carbohydrate accumulation is a ...

journal_title：Journal of bacteriology

authors： Lillie SH,Pringle JR

更新日期：1980-09-01 00:00:00

abstract：:The involvement of the recently characterized 5' exonuclease activity of RNase J1 and endonuclease activity of RNase Y in the turnover of ΔermC mRNA in Bacillus subtilis was investigated. Evidence is presented that both of these activities determine the half-life of ΔermC mRNA. ...

journal_title：Journal of bacteriology

authors： Yao S,Richards J,Belasco JG,Bechhofer DH

更新日期：2011-11-01 00:00:00

abstract：:The content and distribution of autolysin were measured in temperature-sensitive morphological mutants of Bacillus subtilis. Strains RUB1000 and RUB1012 grew as rods at 30 C. At 45 C the mutants contained disproportionately less teichoic acid than peptidoglycan and grew as irregular spheres. The amount of enzyme that ...

journal_title：Journal of bacteriology

authors： Brown WC,Wilson CR,Lukehart S,Young FE,Shiflett MA

更新日期：1976-01-01 00:00:00

abstract：:The structural genes of ADPglucose pyrophosphorylase (glgC) and glycogen synthase (glgA) from Salmonella typhimurium LT2 were cloned on a 5.8-kilobase-pair insert in the SalI site of pBR322. A single strand specific radioactive probe containing the N terminus of the Escherichia coli K-12 glgC gene in M13mp8 was used t...

journal_title：Journal of bacteriology

authors： Leung PS,Preiss J

更新日期：1987-09-01 00:00:00

abstract：:The ability to form type 1 fimbriae (Fim(+)) was readily transduced to 159 out of 161 wild-type motile Fim(-) FIRN strains of Salmonella typhimurium with phage P22 propagated on a Fim(+) donor strain. Fim(+) clones were isolated from about 35% of tests after the fimbriate bacteria in the transduction mixture had been ...

journal_title：Journal of bacteriology

authors： Old DC,Duguid JP

更新日期：1971-09-01 00:00:00

abstract：:On the basis of the constitutive phenotypes of two catM mutants of Acinetobacter calcoaceticus, the CatM protein was proposed to repress expression of two different loci involved in catechol degradation, catA and catBCIJFD (E. Neidle, C. Hartnett, and L. N. Ornston, J. Bacteriol. 171:5410-5421, 1989). In spite of its ...

journal_title：Journal of bacteriology

authors： Romero-Arroyo CE,Schell MA,Gaines GL 3rd,Neidle EL

更新日期：1995-10-01 00:00:00

abstract：:Expression of the pneumococcal type 1 pilus is bistable and positively regulated by the transcription factor RlrA. RlrA is also known to positively control its own expression. Here we present evidence that bistable expression of the type 1 pilus is mediated by the positive-feedback loop controlling rlrA expression. ...

journal_title：Journal of bacteriology

authors： Basset A,Turner KH,Boush E,Sayeed S,Dove SL,Malley R

更新日期：2012-03-01 00:00:00

abstract：:Adenylate cyclases (ACs) catalyze the formation of 3',5'-cyclic AMP (cAMP) from ATP. A novel AC-encoding gene, cyaC, was isolated from Rhizobium etli by phenotypic complementation of an Escherichia coli cya mutant. The functionality of the cyaC gene was corroborated by its ability to restore cAMP accumulation in an E....

journal_title：Journal of bacteriology

authors： Téllez-Sosa J,Soberón N,Vega-Segura A,Torres-Márquez ME,Cevallos MA

更新日期：2002-07-01 00:00:00

abstract：:This study describes the isolation and characterization of a unique class of TolC mutants that, under steady-state growth conditions, secreted normal levels of largely inactive alpha-hemolysin. Unlike the reduced activity in the culture supernatants, the cell-associated hemolytic activity in these mutants was identica...

journal_title：Journal of bacteriology

authors： Vakharia H,German GJ,Misra R

更新日期：2001-12-01 00:00:00

abstract：:Protoplast fusion between the Rec- mutant RN981 (L. Wyman, R. V. Goering, and R. P. Novick, Genetics 76:681-702, 1974) of Staphylococcus aureus NCTC 8325 and a Rec+ NCTC 8325 derivative yielded Rec+ recombinants that exhibited the increased sensitivity to N-methyl-N'-nitro-N-nitrosoguanidine characteristic of RN981. T...

journal_title：Journal of bacteriology

authors： Tam JE,Pattee PA

更新日期：1986-11-01 00:00:00