Deoxyribonucleate binding and transformation in Rhizobium jpaonicum.

Abstract:

:Rhizobium japonicum, capable of binding high-molecular-weight donor (32)P-labeled deoxyribonucleic acid (DNA) during late log phase in a competence medium, was transformed for streptomycin resistance with a frequency of transformation ranging between 0.02 and 0.08%. Eight to 10% of the homologous native (32)P-labeled input DNA was bound irreversibly in a temperature-dependent manner. Homologous denatured (32)P-labeled DNA was incapable of binding to the recipient under similar conditions. CsCl density gradient banding of the donor and recipient DNA indicated homology. The low frequency of transformation could be due to one or more steps that occur between DNA uptake and integration.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Raina JL,Modi VV

doi

10.1128/JB.111.2.356-360.1972

subject

Has Abstract

pub_date

1972-08-01 00:00:00

pages

356-60

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

111

pub_type

杂志文章
  • Salmonella typhimurium mutants generally defective in chemotaxis.

    abstract::The mutations of eight chemotaxis-deficient strains of Salmonella typhimurium, including five new mutants in strain LT2, were mapped by P22 transduction in relation to various fla mot deletions in S. abortus-equi. Seven recessive che mutations mapped between motB and flaC: three, all nontumbling, the che region I, adj...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.128.3.754-765.1976

    authors: Collins AL,Stocker BA

    更新日期:1976-12-01 00:00:00

  • SigmaS controls multiple pathways associated with intracellular multiplication of Legionella pneumophila.

    abstract::Legionella pneumophila is the causative agent of the severe and potentially fatal pneumonia Legionnaires' disease. L. pneumophila is able to replicate within macrophages and protozoa by establishing a replicative compartment in a process that requires the Icm/Dot type IVB secretion system. The signals and regulatory p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01578-08

    authors: Hovel-Miner G,Pampou S,Faucher SP,Clarke M,Morozova I,Morozov P,Russo JJ,Shuman HA,Kalachikov S

    更新日期:2009-04-01 00:00:00

  • Regulation by a novel protein of the bimodal distribution of lipopolysaccharide in the outer membrane of Escherichia coli.

    abstract::We report on the cloning and characterization of the rfb gene cluster of the O75 lipopolysaccharide from a urinary tract isolate of Escherichia coli. Deletion cloning defined the minimum region of DNA that expressed the O75 antigen in E. coli host strains to be on a 12.4-kb insert. However, the E. coli strain expressi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.18.5699-5704.1991

    authors: Batchelor RA,Haraguchi GE,Hull RA,Hull SI

    更新日期:1991-09-01 00:00:00

  • Use of ultraviolet radiation to locate dipicolinic acid in Bacillus cereus spores.

    abstract::A novel method is described that allows a direct determination of the location of a portion of the dipicolinic acid (DPA) in spores. Ultraviolet (UV) irradiation is shown to cause cross-linkage of DPA to spore proteins which have the characteristics of membrane proteins. We suggest that DPA resides in the inner foresp...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.118.1.202-208.1974

    authors: Germaine GR,Murrell WG

    更新日期:1974-04-01 00:00:00

  • Hydroxy fatty acids in Bacteroides species: D-(--)-3-hydroxy-15-methylhexadecanoate and its homologs.

    abstract::Acid hydrolysates of 140 strains, representing 11 species of the genus Bacteroides, were analyzed by capillary gas-liquid chromatography for total cellular fatty acid. All samples contained components which appeared to be hydroxy fatty acid. The relative amount and chain length distribution of the hydroxy fatty acids,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.143.2.582-587.1980

    authors: Mayberry WR

    更新日期:1980-08-01 00:00:00

  • Uracil-DNA glycosylase of Thermoplasma acidophilum directs long-patch base excision repair, which is promoted by deoxynucleoside triphosphates and ATP/ADP, into short-patch repair.

    abstract::Hydrolytic deamination of cytosine to uracil in DNA is increased in organisms adapted to high temperatures. Hitherto, the uracil base excision repair (BER) pathway has only been described in two archaeons, the crenarchaeon Pyrobaculum aerophilum and the euryarchaeon Archaeoglobus fulgidus, which are hyperthermophiles ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00233-11

    authors: Moen MN,Knævelsrud I,Haugland GT,Grøsvik K,Birkeland NK,Klungland A,Bjelland S

    更新日期:2011-09-01 00:00:00

  • Functional characterization of a novel ArgA from Mycobacterium tuberculosis.

    abstract::The Mycobacterium tuberculosis gene Rv2747 encodes a novel 19-kDa ArgA that catalyzes the initial step in L-arginine biosynthesis, namely the conversion of L-glutamate to alpha-N-acetyl-L-glutamate. Initial velocity studies reveal that Rv2747 proceeds through a sequential kinetic mechanism, with K(m) values of 280 mM ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.9.3039-3044.2005

    authors: Errey JC,Blanchard JS

    更新日期:2005-05-01 00:00:00

  • Genetic mapping of katG, a locus that affects synthesis of the bifunctional catalase-peroxidase hydroperoxidase I in Escherichia coli.

    abstract::A locus unlinked to either katE or katF that affected catalase levels in Escherichia coli was identified and localized between metB and ppc at 89.2 min on the genome. The locus was named katG. Mutations in katG which prevented the formation of both isoenzyme forms of the bifunctional catalase-peroxidase HPI were creat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.162.2.661-667.1985

    authors: Loewen PC,Triggs BL,George CS,Hrabarchuk BE

    更新日期:1985-05-01 00:00:00

  • Subunits of succinyl-coenzyme A synthetase: coordination of production in Escherichia coli and discovery of a factor that precludes refolding.

    abstract::Succinyl-coenzyme A synthetase of Escherichia coli has an alpha 2 beta 2 subunit structure. By measuring reconstituted enzyme activity present after addition of purified alpha or beta subunits to cell extracts followed by refolding, we have shown that extracts contain no significant excess of either subunit species. T...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.143.1.231-237.1980

    authors: Wolodko WT,Brownie ER,Bridger WA

    更新日期:1980-07-01 00:00:00

  • Plasmid virulence gene expression induced by short-chain fatty acids in Salmonella dublin: identification of rpoS-dependent and rpo-S-independent mechanisms.

    abstract::The Salmonella plasmid virulence spvABCD genes are growth phase regulated and require RpoS for maximal expression in stationary phase. We identified a growth phase-independent expression of spv which is mediated by short-chain fatty acids. During this fatty acid-mediated expression of spv, RpoS is required for inducti...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.4.1409-1412.1997

    authors: El-Gedaily A,Paesold G,Chen CY,Guiney DG,Krause M

    更新日期:1997-02-01 00:00:00

  • Transcription of the archaeal trkA homolog in Methanosarcina mazei S-6.

    abstract::Transcription of the archaeal trkA gene homolog in Methanosarcina mazei S-6 was studied at the optimal growth temperature of 37 degrees C and after heat shock at 45 degrees C. Northern (RNA) blotting results (transcript size) and data from primer extension experiments to map the transcription initiation site indicate ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.21.6077-6082.1995

    authors: Conway de Macario E,Macario AJ

    更新日期:1995-11-01 00:00:00

  • Multifaceted Interfaces of Bacterial Competition.

    abstract::Microbial communities span many orders of magnitude, ranging in scale from hundreds of cells on a single particle of soil to billions of cells within the lumen of the gastrointestinal tract. Bacterial cells in all habitats are members of densely populated local environments that facilitate competition between neighbor...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章,评审

    doi:10.1128/JB.00275-16

    authors: Stubbendieck RM,Straight PD

    更新日期:2016-07-28 00:00:00

  • Carotenoids acts as reinforcers of the Acholeplasma laidlawii lipid bilayer.

    abstract::Acholeplasma laidlawii cells grown with oleic acid produced much more colored carotenoids than did cells grown with elaidic acid. The amount of carotenoids was decreased 80 to 90% by growing the cells with 0.05 M propionate, resulting in a marked increase in the mobility of both 5-doxylstearate and 12-doxylstearate in...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.140.3.944-948.1979

    authors: Rottem S,Markowitz O

    更新日期:1979-12-01 00:00:00

  • Induction and repression of nitrate reductase in Neurospora crassa.

    abstract::Kinsky, Stephen C. (Washington University, St. Louis, Mo.). Induction and repression of nitrate reductase in Neurospora crassa. J. Bacteriol. 82:898-904. 1961.-Techniques are described for studying induced enzyme formation in Neurospora crassa. The effects of various parameters (time, pH, nitrate concentration, etc.) ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.82.6.898-904.1961

    authors: KINSKY SC

    更新日期:1961-12-01 00:00:00

  • Overproduction of SecA suppresses the export defect caused by a mutation in the gene encoding the Escherichia coli export chaperone secB.

    abstract::SecB is a cytosolic protein required for rapid and efficient export of particular periplasmic and outer membrane proteins in Escherichia coli. SecB promotes export by stabilizing newly synthesized precursor proteins in a nonnative conformation and by targeting the precursors to the inner membrane. Biochemical studies ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.10.3010-3017.1999

    authors: Cook HA,Kumamoto CA

    更新日期:1999-05-01 00:00:00

  • The ompB operon partially determines differential expression of OmpC in Salmonella typhi and Escherichia coli.

    abstract::Expression of the Escherichia coli OmpC and OmpF outer membrane proteins is regulated by the osmolarity of the culture media. In contrast, expression of OmpC in Salmonella typhi is not influenced by osmolarity, while OmpF is regulated as in E. coli. To better understand the lack of osmoregulation of OmpC expression in...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.2.556-562.1999

    authors: Martínez-Flores I,Cano R,Bustamante VH,Calva E,Puente JL

    更新日期:1999-01-01 00:00:00

  • Isolation and characterization of an Escherichia coli mutant affected in the regulation of adenylate cyclase.

    abstract::A mutant, cyaR1, affecting regulation of adenylate cyclase expression or activity is described. It was obtained as a thermoresistant revertant of a strain harboring a thermosensitive transcription termination factor, rho (rho-15). This mutant failed to synthesize adenosine 3',5'-phosphate and exhibited a carbohydrate-...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.148.3.753-761.1981

    authors: Guidi-Rontani C,Danchin A,Ullmann A

    更新日期:1981-12-01 00:00:00

  • Ultraviolet- and X-ray-induced responses of a deoxyribonucleic acid polymerase-deficient mutant of Escherichia coli.

    abstract::Escherichia coli K-12, polAl(-) is a mutant strain whose extracts are deficient in Kornberg deoxyribonucleic acid (DNA) polymerase activity. We have compared the mutant and parental strains on the basis of a number of responses to ultraviolet (UV) and X-irradiation. For both types of radiation, the mutant is more sens...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.107.1.61-67.1971

    authors: Paterson MC,Boyle JM,Setlow RB

    更新日期:1971-07-01 00:00:00

  • Biosynthesis of riboflavin in Bacillus subtilis: function and genetic control of the riboflavin synthase complex.

    abstract::Two riboflavin synthase activities (heavy and light) have been observed in earlier studies with Bacillus subtilis. The heavy enzyme is a complex of one molecule of light enzyme (consisting of three alpha subunits) and approximately 60 beta subunits (A. Bacher, R. Bauer, U. Eggers, H. Harders, and H. Schnepple, p. 729-...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.134.2.476-482.1978

    authors: Bacher A,Mailänder B

    更新日期:1978-05-01 00:00:00

  • Effects of various drugs on hemolytic activity of Streptococcus zymogenes.

    abstract::Drugs known to interrupt the chain of protein synthesis (streptomycin, chloramphenicol, ethidium bromide) appeared to affect production of the group D lysin by Streptococcus zymogenes by inhibiting growth. Drugs which block cell wall synthesis (vancomycin, bacitracin, d-cycloserine, and phosphonomycin) inhibited lytic...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.108.2.844-848.1971

    authors: Werth JM,Jackson RW

    更新日期:1971-11-01 00:00:00

  • Genetic construction and functional analysis of hybrid polyketide synthases containing heterologous acyl carrier proteins.

    abstract::The gene that encodes the acyl carrier protein (ACP) of the actinorhodin polyketide synthase (PKS) of Streptomyces coelicolor A3(2) was replaced with homologs from the granaticin, oxytetracycline, tetracenomycin, and putative frenolicin polyketide synthase gene clusters. All of the replacements led to expression of fu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.8.2197-2204.1993

    authors: Khosla C,McDaniel R,Ebert-Khosla S,Torres R,Sherman DH,Bibb MJ,Hopwood DA

    更新日期:1993-04-01 00:00:00

  • Formation of bacteriolytic enzymes in batch and continuous culture of Staphylococcus aureus.

    abstract::The formation of bacteriolytic enzymes of Staphylococcus aureus, with special reference to strain M18, was investigated under a variety of conditions. The bacteriolytic activity was tested by using whole cells of Micrococcus lysodeikticus as a substrate. Complex media were required for production, and a Casein Hydroly...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.104.1.227-233.1970

    authors: Arvidson S,Holme T,Wadström T

    更新日期:1970-10-01 00:00:00

  • Bacteria of the genus Bacillus have a hydrolase stereospecific to the D isomer of benzoyl-arginine-p-nitroanilide.

    abstract::A stereospecific enzyme activity capable of cleaving the amide bond of the synthetic substrate N-benzoyl-D-arginine-p-nitroanilide (D-BAPA) has been found in all aerobic and anaerobic members of the family Bacillaceae tested by us. Cells of nonsporeforming gram-positive or gram-negative bacteria contain a hydrolase ac...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.12.5895-5900.1988

    authors: Gofshtein-Gandman LV,Keynan A,Milner Y

    更新日期:1988-12-01 00:00:00

  • Molecular cloning and regulation of expression of the genes for initiation factor 3 and two aminoacyl-tRNA synthetases.

    abstract::A 22-kilobase fragment of the Escherichia coli chromosome which contains the genes for translation initiation factor 3, phenylalanyl-tRNA synthetase, and threonyl-tRNA synthetase was cloned into plasmid pACYC184. The hybrid plasmid (designated pID1) complements a temperature-sensitive pheS lesion in E. coli NP37. pID1...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:

    authors: Elseviers D,Gallagher P,Hoffman A,Weinberg B,Schwartz I

    更新日期:1982-10-01 00:00:00

  • Release of proteinase from mycelium of Mucor hiemalis.

    abstract::When Mucor hiemalis NRRL 3103 was grown in soybean medium, only a small fraction of the proteinase produced by the organism appeared in the culture filtrate, whereas the bulk of the enzyme was bound to the mycelial surface. Optimal pH of the proteinase ranged from 3.0 to 3.5. Inclusion of sodium chloride or other ioni...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.93.6.1794-1799.1967

    authors: Wang HL

    更新日期:1967-06-01 00:00:00

  • Transcription of the ileS operon in the archaeon Methanobacterium thermoautotrophicum Marburg.

    abstract::In the thermophilic archaeon Methanobacterium thermoautotrophicum Marburg, the structural gene for isoleucyl-tRNA synthetase (ileS) is flanked upstream by orf401 and downstream by purL. orf401 encodes a 43.5-kDa protein with an unknown function. Northern (RNA) hybridization and S1 nuclease protection experiments showe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.18.5945-5952.1993

    authors: Jenal U,Thurner C,Leisinger T

    更新日期:1993-09-01 00:00:00

  • Adaptation of Mycobacterium smegmatis to stationary phase.

    abstract::Mycobacterium tuberculosis can persist for many years within host lung tissue without causing clinical disease. Little is known about the state in which the bacilli survive, although it is frequently referred to as dormancy. Some evidence suggests that cells survive in nutrient-deprived stationary phase. Therefore, we...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.1.270-283.1999

    authors: Smeulders MJ,Keer J,Speight RA,Williams HD

    更新日期:1999-01-01 00:00:00

  • Transcriptional activation of the nitrogenase promoter in vitro: adenosine nucleotides are required for inhibition of NIFA activity by NIFL.

    abstract::The enhancer-binding protein NIFA is required for transcriptional activation of nif promoters by the alternative holoenzyme form of RNA polymerase, which contains the sigma factor sigma 54 (sigma N). NIFA hydrolyzes nucleoside triphosphates to catalyze the isomerization of closed promoter complexes to transcriptionall...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.5.1186-1195.1995

    authors: Eydmann T,Söderbäck E,Jones T,Hill S,Austin S,Dixon R

    更新日期:1995-03-01 00:00:00

  • Cloning, nucleotide sequence, and hybridization studies of the type IIb heat-labile enterotoxin gene of Escherichia coli.

    abstract::Type IIb heat-labile enterotoxin (LT-IIb) is produced by Escherichia coli 41. Restriction fragments of total cell DNA from strain 41 were cloned into a cosmid vector, and one cosmid clone that encoded LT-IIb was identified. The genes for LT-IIb were subcloned into a variety of plasmids, expressed in minicells, sequenc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.9.4945-4952.1989

    authors: Pickett CL,Twiddy EM,Coker C,Holmes RK

    更新日期:1989-09-01 00:00:00

  • Isolation of a high-affinity functional protein complex between OmcA and MtrC: Two outer membrane decaheme c-type cytochromes of Shewanella oneidensis MR-1.

    abstract::Shewanella oneidensis MR-1 is a facultatively anaerobic bacterium capable of using soluble and insoluble forms of manganese [Mn(III/IV)] and iron [Fe(III)] as terminal electron acceptors during anaerobic respiration. To assess the structural association of two outer membrane-associated c-type decaheme cytochromes (i.e...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01966-05

    authors: Shi L,Chen B,Wang Z,Elias DA,Mayer MU,Gorby YA,Ni S,Lower BH,Kennedy DW,Wunschel DS,Mottaz HM,Marshall MJ,Hill EA,Beliaev AS,Zachara JM,Fredrickson JK,Squier TC

    更新日期:2006-07-01 00:00:00