Abstract:
:A stereospecific enzyme activity capable of cleaving the amide bond of the synthetic substrate N-benzoyl-D-arginine-p-nitroanilide (D-BAPA) has been found in all aerobic and anaerobic members of the family Bacillaceae tested by us. Cells of nonsporeforming gram-positive or gram-negative bacteria contain a hydrolase activity stereospecific to N-benzoyl-L-arginine-p-nitroanilide. The D-BAPA-hydrolyzing enzymes (D-BAPAases) of mid-logarithmic-phase cells of Bacillus subtilis 168 and B. cereus T were compared. These enzymes had the same molecular weight of approximately 66,000 in gel filtration and the same electrophoretic mobility after electrophoresis on polyacrylamide gels. The D-BAPAases of B. subtilis 168 and B. cereus T differed in the effect of inhibitors on enzymatic activity. While both hydrolases were inhibited by tosyl-L-lysine chloromethyl ketone and tosyl-L-arginine-methyl ester as well as leupeptin, only the D-BAPAase of B. cereus T was inhibited by p-chloromercuribenzene sulfonic acid. The D-BAPAases of B. subtilis and B. cereus T had a Michaelis constant for D-BAPA of 2.9 x 10(-5) M and 1.4 x 10(-4) M, respectively. D-BAPAase is an intracellular enzyme localized in the protoplast (80 to 90% in soluble form in the cytoplasm). The ability to cleave D-BAPA is suggested as an additional chemotaxonomic characteristic of sporeforming bacteria of the genera Bacillus and Clostridium.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Gofshtein-Gandman LV,Keynan A,Milner Ydoi
10.1128/jb.170.12.5895-5900.1988subject
Has Abstractpub_date
1988-12-01 00:00:00pages
5895-900issue
12eissn
0021-9193issn
1098-5530journal_volume
170pub_type
杂志文章abstract::The Brevibacterium acetylicum gsk gene, which encodes guanosine kinase (ATP:guanosine 5'-phosphotransferase), a kinase that is involved in guanosine salvage pathways, has been cloned by using the N-terminal amino acid sequence of the purified protein. The cloned chromosomal fragment containing the gsk gene was sequenc...
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journal_title:Journal of bacteriology
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doi:10.1128/JB.126.1.539-541.1976
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doi:10.1128/JB.86.5.1041-1051.1963
更新日期:1963-11-01 00:00:00
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更新日期:2003-03-01 00:00:00
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更新日期:2005-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.12.3614-3630.1994
更新日期:1994-06-01 00:00:00
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更新日期:1971-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1991-10-01 00:00:00
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更新日期:1979-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1982-09-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/jb.179.22.7111-7117.1997
更新日期:1997-11-01 00:00:00
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更新日期:1987-06-01 00:00:00
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更新日期:1996-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.11.2842-2848.1998
更新日期:1998-06-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:2010-03-01 00:00:00
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