Disulfide bonding within components of the Chlamydia type III secretion apparatus correlates with development.

Abstract:

:Chlamydia spp. exhibit a unique biphasic developmental cycle whereby infectious elementary bodies (EBs) invade host epithelial cells and differentiate into noninfectious, metabolically active reticulate bodies (RBs). EBs posses a unique outer envelope where rigidity is achieved by disulfide bonding among cysteine-rich envelope-associated proteins. Conversely, these disulfide bonds become reduced in RBs to accommodate vegetative growth, thereby linking the redox status of cysteine-rich envelope proteins with progression of the developmental cycle. We investigated the potential role of disulfide bonding within the chlamydial type III secretion system (T3SS), since activity of this system is also closely linked to development. We focused on structural components of the T3S apparatus that contain an unusually high number of cysteine residues compared to orthologs in other secretion systems. Nonreducing SDS-PAGE revealed that EB-localized apparatus proteins such as CdsF, CdsD, and CdsC form higher-order complexes mediated by disulfide bonding. The most dramatic alterations were detected for the needle protein CdsF. Significantly, disulfide bonding patterns shifted during differentiation of developmental forms and were completely reduced in RBs. Furthermore, at later time points during infection following RB to EB conversion, we found that CdsF is reoxidized into higher-order complexes. Overall, we conclude that the redox status of specific T3SS apparatus proteins is intimately linked to the developmental cycle and constitutes a newly appreciated aspect of functionally significant alterations within proteins of the chlamydial envelope.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Betts-Hampikian HJ,Fields KA

doi

10.1128/JB.05163-11

subject

Has Abstract

pub_date

2011-12-01 00:00:00

pages

6950-9

issue

24

eissn

0021-9193

issn

1098-5530

pii

JB.05163-11

journal_volume

193

pub_type

杂志文章
  • In vivo and in vitro kinetics of nitrogenase.

    abstract::We measured some of the kinetic parameters of nitrogenase to intact systems of Clostridium pasteurianum and Klebsiella pneumoniae to compare them with the kinetics of the enzyme in vitro. We found that the enzyme showed multiple apparent Km values for acetylene reduction in vivo, as it does in vitro. Carbon monoxide w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.141.3.1230-1238.1980

    authors: Davis LC,Wang YL

    更新日期:1980-03-01 00:00:00

  • Differential proteomic analysis of the Bacillus anthracis secretome: distinct plasmid and chromosome CO2-dependent cross talk mechanisms modulate extracellular proteolytic activities.

    abstract::The secretomes of a virulent Bacillus anthracis strain and of avirulent strains (cured of the virulence plasmids pXO1 and pXO2), cultured in rich and minimal media, were studied by a comparative proteomic approach. More than 400 protein spots, representing the products of 64 genes, were identified, and a unique patter...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.10.3551-3571.2006

    authors: Chitlaru T,Gat O,Gozlan Y,Ariel N,Shafferman A

    更新日期:2006-05-01 00:00:00

  • The metD D-methionine transporter locus of Escherichia coli is an ABC transporter gene cluster.

    abstract::The metD D-methionine transporter locus of Escherichia coli was identified as the abc-yaeE-yaeC cluster (now renamed metNIQ genes). The abc open reading frame is preceded by tandem MET boxes bracketed by the -10 and -35 boxes of a promoter. The expression driven by this promoter is controlled by the MetJ repressor and...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.17.4930-4932.2002

    authors: Gál J,Szvetnik A,Schnell R,Kálmán M

    更新日期:2002-09-01 00:00:00

  • Negative regulation of expression of the nitrate assimilation nirA operon in the heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120.

    abstract::In the filamentous, heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120, expression of the nitrate assimilation nirA operon takes place in the absence of ammonium and the presence of nitrate or nitrite. Several positive-action proteins that are required for expression of the nirA operon have been identified...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01668-09

    authors: Frías JE,Flores E

    更新日期:2010-06-01 00:00:00

  • Overproduction of Escherichia coli integration host factor, a protein with nonidentical subunits.

    abstract::Integration host factor (IHF) is a small, basic protein that is needed for efficient recombination of bacteriophage lambda, as well as for other host and viral functions. We have constructed strains in which the two subunits of IHF, encoded by the himA and hip genes of Escherichia coli, are expressed under the control...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.9.4124-4127.1987

    authors: Nash HA,Robertson CA,Flamm E,Weisberg RA,Miller HI

    更新日期:1987-09-01 00:00:00

  • MULTIPLE CELLULASE SYSTEM FROM STREPTOMYCES ANTIBIOTICUS.

    abstract::Enger, M. D. (North Dakota State University, Fargo), and B. P. Sleeper. Multiple cellulase system from Streptomyces antibioticus. J. Bacteriol. 89:23-27. 1965.-Starch-block zone electrophoresis was used to isolate five electrophoretically distinct, active cellulolytic components (I to V) from the crude extracellular c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.89.1.23-27.1965

    authors: ENGER MD,SLEEPER BP

    更新日期:1965-01-01 00:00:00

  • Excretion of unassembled hook-associated proteins by Salmonella typhimurium.

    abstract::Hook-associated proteins (HAPs) were excreted into the culture medium of the Fla+ strain as well as into the growth medium of the filamentless mutants of Salmonella typhimurium. This indicates that the bacteria synthesize HAPs excessively, beyond the amount required for construction of flagella. The extra HAPs are she...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.164.3.1370-1372.1985

    authors: Homma M,Iino T

    更新日期:1985-12-01 00:00:00

  • nodO, a new nod gene of the Rhizobium leguminosarum biovar viciae sym plasmid pRL1JI, encodes a secreted protein.

    abstract::The region of the Rhizobium leguminosarum biovar viciae Sym plasmid pRL1JI, responsible for the production and secretion of a previously described 50-kilodalton protein (R. A. de Maagd, C. A. Wijffelman, E. Pees, and B. J. J. Lugtenberg, J. Bacteriol. 170:4424-4427, 1988), was cloned and its nucleotide sequence was de...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.12.6764-6770.1989

    authors: de Maagd RA,Wijfjes AH,Spaink HP,Ruiz-Sainz JE,Wijffelman CA,Okker RJ,Lugtenberg BJ

    更新日期:1989-12-01 00:00:00

  • The pi-histidine factor of Salmonella typhimurium: a demonstration that pi-histidine factor integrates into the chromosome.

    abstract::The Salmonella typhimurium pi-histidine episome was identified by Ames et al. (2) in an unstable partial revertant of a deletion mutation-containing strain, hisG203. HisG203 lacks the histidine operator, promoter, and part of the first structural gene. In this paper, we study some properties of pi factor and demonstra...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.109.3.993-1000.1972

    authors: Levinthal M,Yeh J

    更新日期:1972-03-01 00:00:00

  • Cloning and characterization of the katA gene of Rhizobium meliloti encoding a hydrogen peroxide-inducible catalase.

    abstract::To investigate the involvement of bacterial catalases of the symbiotic gram-negative bacterium Rhizobium meliloti in the development of Medicago-Rhizobium functional nodules, we cloned a putative kat gene by screening a cosmid library with a catalase-specific DNA probe amplified by PCR from the R. meliloti genome. Nuc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.23.6802-6809.1996

    authors: Hérouart D,Sigaud S,Moreau S,Frendo P,Touati D,Puppo A

    更新日期:1996-12-01 00:00:00

  • Role of the dinB gene product in spontaneous mutation in Escherichia coli with an impaired replicative polymerase.

    abstract::We isolated several new mutator mutations of the Escherichia coli replicative polymerase dnaE subunit alpha and used them and a previously reported dnaE mutation to study spontaneous frameshift and base substitution mutations. Two of these dnaE strains produce many more mutants when grown on rich (Luria-Bertani) than ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.23.6742-6750.2000

    authors: Strauss BS,Roberts R,Francis L,Pouryazdanparast P

    更新日期:2000-12-01 00:00:00

  • Conjugative plasmids of enteric bacteria from many different incompatibility groups have similar genes for single-stranded DNA-binding proteins.

    abstract::Among 30 conjugative plasmids of enteric bacteria from 23 incompatibility (Inc) groups, we found 19 (from 12 Inc groups) which can complement defects caused by a defective single-stranded DNA-binding protein of Escherichia coli K-12. The genes which are responsible for the complementation from three of these plasmids ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.162.1.235-241.1985

    authors: Golub EI,Low KB

    更新日期:1985-04-01 00:00:00

  • Long-chain fatty acid inhibition of growth of Streptococcus agalactiae in a chemically defined medium.

    abstract::Willett, Norman P. (University of Pennsylvania School of Veterinary Medicine, Kennett Square, Pa.), and Guy E. Morse. Long-chain fatty acid inhibition of growth of Streptococcus agalactiae in a chemically defined medium. J. Bacteriol. 91:2245-2250. 1966.-A chemically defined medium was developed for Streptococcus agal...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.6.2245-2250.1966

    authors: Willett NP,Morse GE

    更新日期:1966-06-01 00:00:00

  • Quantitative assay of polysaccharide components obtained from cell wall lipopolysaccharides of Xanthomonas species.

    abstract::The cell wall lipopolysaccharides from 17 species belonging to the genus Xanthomonas were extracted from the cells with hot 45% phenol. After purification, the components of the polysaccharide were obtained by acid hydrolysis of the lipopolysaccharide and were quantitatively assayed. Data obtained show that all prepar...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.3.980-982.1968

    authors: Volk WA

    更新日期:1968-03-01 00:00:00

  • Transcriptional organization of the phycocyanin subunit gene clusters of the cyanobacterium Anacystis nidulans UTEX 625.

    abstract::The phycocyanin subunit gene cluster is duplicated on the chromosome of the cyanobacterium Anacystis nidulans UTEX 625. The two gene clusters cpcB1A1 (left) and cpcB2A2 (right) are separated by about 2,500 base pairs, and in each cluster the beta-subunit gene is located upstream from the alpha-subunit gene. Filter hyb...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.7.2961-2970.1988

    authors: Kalla SR,Lind LK,Lidholm J,Gustafsson P

    更新日期:1988-07-01 00:00:00

  • Reassociation of purified lipopolysaccharide and phospholipid of the bacterial cell envelope: electron microscopic and monolayer studies.

    abstract::Phosphatidyl ethanolamine and lipopolysaccharide were extracted and purified from the cell envelope fractions of Escherichia coli and Salmonella typhimurium. The two components were studied separately and after recombination, by use of electron microscopy and monolayer techniques, and by measuring their ability to par...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.93.5.1705-1721.1967

    authors: Rothfield L,Horne RW

    更新日期:1967-05-01 00:00:00

  • Analysis of storage lipid accumulation in Alcanivorax borkumensis: Evidence for alternative triacylglycerol biosynthesis routes in bacteria.

    abstract::Marine hydrocarbonoclastic bacteria, like Alcanivorax borkumensis, play a globally important role in bioremediation of petroleum oil contamination in marine ecosystems. Accumulation of storage lipids, serving as endogenous carbon and energy sources during starvation periods, might be a potential adaptation mechanism f...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01292-06

    authors: Kalscheuer R,Stöveken T,Malkus U,Reichelt R,Golyshin PN,Sabirova JS,Ferrer M,Timmis KN,Steinbüchel A

    更新日期:2007-02-01 00:00:00

  • Impact of either elevated or decreased levels of cytochrome bd expression on Shigella flexneri virulence.

    abstract::Shigella spp. are the major cause of bacillary dysentery worldwide. The pathogenic process involves bacterial invasion and lysis of the phagocytic vacuole, followed by replication and movement within the cell cytoplasm and, ultimately, spread directly into adjacent cells. This study demonstrates that S. flexneri cytoc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.4.1229-1237.1999

    authors: Way SS,Sallustio S,Magliozzo RS,Goldberg MB

    更新日期:1999-02-01 00:00:00

  • Variable enzymological patterning in tyrosine biosynthesis as a means of determining natural relatedness among the Pseudomonadaceae.

    abstract::Enzymes of tyrosine biosynthesis (prephenate dehydrogenase and arogenate dehydrogenase) were characterized in 90 species currently classified within the genera Pseudomonas, Xanthomonas, and Alcaligenes. Variation in cofactor specificity and regulatory properties of the dehydrogenase proteins allowed the separation of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.144.1.247-257.1980

    authors: Byng GS,Whitaker RJ,Gherna RL,Jensen RA

    更新日期:1980-10-01 00:00:00

  • Plasmid-encoded regulator of extracellular proteases in Bacillus anthracis.

    abstract::Bacillus anthracis Sterne cured of the pXO1 plasmid had enhanced secreted protease activity during the postexponential phase but no change in hemolytic or lecithinase activities. A zymogen profile revealed at least six proteases, including serine, metal, and perhaps cysteine types. There were similar amounts of protea...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.9.3133-3138.2005

    authors: Aronson AI,Bell C,Fulroth B

    更新日期:2005-05-01 00:00:00

  • New mutation (mmrA1) in Escherichia coli K-12 that affects minimal medium recovery and postreplication repair after UV irradiation.

    abstract::After UV irradiation, Escherichia coli uvrA mutant cells show higher survival on minimal than on rich growth medium, i.e., they show minimal-medium recovery. This effect of rich growth medium on survival is not observed in a uvrA mutant carrying an mmrA1 mutation, and the uvrA mmrA strain showed the same survival rate...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.154.2.743-747.1983

    authors: Sharma RC,Sargentini NJ,Smith KC

    更新日期:1983-05-01 00:00:00

  • UDP-glucuronic acid decarboxylases of Bacteroides fragilis and their prevalence in bacteria.

    abstract::Xylose is rarely described as a component of bacterial glycans. UDP-xylose is the nucleotide-activated form necessary for incorporation of xylose into glycans and is synthesized by the decarboxylation of UDP-glucuronic acid (UDP-GlcA). Enzymes with UDP-GlcA decarboxylase activity include those that lead to the formati...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05337-11

    authors: Coyne MJ,Fletcher CM,Reinap B,Comstock LE

    更新日期:2011-10-01 00:00:00

  • Cloning and characterization of the gene cluster for palatinose metabolism from the phytopathogenic bacterium Erwinia rhapontici.

    abstract::Erwinia rhapontici is able to convert sucrose into isomaltulose (palatinose, 6-O-alpha-D-glucopyranosyl-D-fructose) and trehalulose (1-O-alpha-D-glucopyranosyl-D-fructose) by the activity of a sucrose isomerase. These sucrose isomers cannot be metabolized by plant cells and most other organisms and therefore are possi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.8.2425-2430.2001

    authors: Börnke F,Hajirezaei M,Sonnewald U

    更新日期:2001-04-01 00:00:00

  • Effect of growth rate on histidine catabolism and histidase synthesis in Aerobacter aerogenes.

    abstract::A study was made of how the catabolism of a carbon and energy source is affected by the biosynthetic demands of growing bacterial cells. Cultures of Aerobacter aerogenes in l-histidine medium were grown in a chemostat at rates determined by the supply of either sulfate or a required amino acid, l-arginine. It was disc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.98.1.131-142.1969

    authors: Jensen DE,Neidhardt FC

    更新日期:1969-04-01 00:00:00

  • EFFECT OF HIGH FAT DIETS ON INTESTINAL MICROFLORA AND SERUM CHOLESTEROL IN RATS.

    abstract::Graber, C. D. (Baylor University College of Medicine, Houston, Tex.), R. M. O'Neal, and E. R. Rabin. Effect of high fat diets on intestinal microflora and serum cholesterol in rats. J. Bacteriol. 89:47-51. 1965.-Differential bacterial counts of feces and total plasma cholesterol determinations were performed on 60 Wis...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.89.1.47-51.1965

    authors: GRABER CD,O'NEAL RM,RABIN ER

    更新日期:1965-01-01 00:00:00

  • Glycine betaine catabolism contributes to Pseudomonas syringae tolerance to hyperosmotic stress by relieving betaine-mediated suppression of compatible solute synthesis.

    abstract::Many bacteria can accumulate glycine betaine for osmoprotection and catabolize it as a growth substrate, but how they regulate these opposing roles is poorly understood. In Pseudomonas syringae B728a, expression of the betaine catabolism genes was reduced by an osmotic upshift to an intermediate stress level, consiste...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00094-13

    authors: Li S,Yu X,Beattie GA

    更新日期:2013-05-01 00:00:00

  • Paraquat regulation of hmp (flavohemoglobin) gene expression in Escherichia coli K-12 is SoxRS independent but modulated by sigma S.

    abstract::We report the first example of a gene, hmp, encoding a soluble flavohemoglobin in Escherichia coli K-12, which is up-regulated by paraquat in a SoxRS-independent manner. Unlike what is found for other paraquat-inducible genes, high concentrations of paraquat (200 microM) were required to increase the level of hmp expr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.10.3164-3170.1997

    authors: Membrillo-Hernández J,Kim SO,Cook GM,Poole RK

    更新日期:1997-05-01 00:00:00

  • Core and panmetabolism in Escherichia coli.

    abstract::Escherichia coli exhibits a wide range of lifestyles encompassing commensalism and various pathogenic behaviors which its highly dynamic genome contributes to develop. How environmental and host factors shape the genetic structure of E. coli strains remains, however, largely unknown. Following a previous study of E. c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01192-10

    authors: Vieira G,Sabarly V,Bourguignon PY,Durot M,Le Fèvre F,Mornico D,Vallenet D,Bouvet O,Denamur E,Schachter V,Médigue C

    更新日期:2011-03-01 00:00:00

  • Neurospora mutant exhibiting hyperproduction of amylase and invertase.

    abstract::A mutant strain of Neurospora crassa has been isolated which is derepressed for amylase and beta-fructofuranosidase (invertase). Large amounts of the two enzymes were secreted into the culture medium upon depletion of exogenous carbon source. The resulting increases of the two extracellular enzymes were prevented by a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.97.2.544-549.1969

    authors: Gratzner H,Sheehan DN

    更新日期:1969-02-01 00:00:00

  • Characterization of high-level expression and sequencing of the Escherichia coli K-12 cynS gene encoding cyanase.

    abstract::Restriction fragments containing the gene encoding cyanase, cynS, without its transcriptional regulatory sequences were placed downstream of lac and tac promoters in various pUC derivatives to maximize production of cyanase. Plasmid pSJ105, which contains the cynS gene and an upstream open reading frame, gave the high...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.11.5224-5230.1987

    authors: Sung YC,Anderson PM,Fuchs JA

    更新日期:1987-11-01 00:00:00