Analysis of storage lipid accumulation in Alcanivorax borkumensis: Evidence for alternative triacylglycerol biosynthesis routes in bacteria.

Abstract:

:Marine hydrocarbonoclastic bacteria, like Alcanivorax borkumensis, play a globally important role in bioremediation of petroleum oil contamination in marine ecosystems. Accumulation of storage lipids, serving as endogenous carbon and energy sources during starvation periods, might be a potential adaptation mechanism for coping with nutrient limitation, which is a frequent stress factor challenging those bacteria in their natural marine habitats. Here we report on the analysis of storage lipid biosynthesis in A. borkumensis strain SK2. Triacylglycerols (TAGs) and wax esters (WEs), but not poly(hydroxyalkanoic acids), are the principal storage lipids present in this and other hydrocarbonoclastic bacterial species. Although so far assumed to be a characteristic restricted to gram-positive actinomycetes, substantial accumulation of TAGs corresponding to a fatty acid content of more than 23% of the cellular dry weight is the first characteristic of large-scale de novo TAG biosynthesis in a gram-negative bacterium. The acyltransferase AtfA1 (ABO_2742) exhibiting wax ester synthase/acyl-coenzyme A:diacylglycerol acyltransferase (WS/DGAT) activity plays a key role in both TAG and WE biosynthesis, whereas AtfA2 (ABO_1804) was dispensable for storage lipid formation. However, reduced but still substantial residual TAG levels in atfA1 and atfA2 knockout mutants compellingly indicate the existence of a yet unknown WS/DGAT-independent alternative TAG biosynthesis route. Storage lipids of A. borkumensis were enriched in saturated fatty acids and accumulated as insoluble intracytoplasmic inclusions exhibiting great structural variety. Storage lipid accumulation provided only a slight growth advantage during short-term starvation periods but was not required for maintaining viability and long-term persistence during extended starvation phases.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Kalscheuer R,Stöveken T,Malkus U,Reichelt R,Golyshin PN,Sabirova JS,Ferrer M,Timmis KN,Steinbüchel A

doi

10.1128/JB.01292-06

subject

Has Abstract

pub_date

2007-02-01 00:00:00

pages

918-28

issue

3

eissn

0021-9193

issn

1098-5530

pii

JB.01292-06

journal_volume

189

pub_type

杂志文章
  • AsnC: an autogenously regulated activator of asparagine synthetase A transcription in Escherichia coli.

    abstract::The regulation of the asparagine synthetase A gene of Escherichia coli was studied in vitro with a coupled transcription-translation system. It was shown that the 17-kilodalton gene, which is transcribed divergently from the adjacent asnA gene, codes for an activator of asnA transcription. The synthesis of the 17-kilo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.164.1.310-315.1985

    authors: Kölling R,Lother H

    更新日期:1985-10-01 00:00:00

  • Stringent regulation of the synthesis of a transfer ribonucleic acid biosynthetic enzyme: transfer ribonucleic acid(m5U)methyltransferase from Escherichia coli.

    abstract::This paper describes the regulation of a transfer ribonucleic acid (tRNA) biosynthetic enzyme, the tRNA(m5U)methyltransferase (EC 2.1.1.35). This enzyme catalyzes the formation of 5-methyluridine (m5U, ribothymidine) in all tRNA chains of Escherichia coli. Partial deprivation of charged tRNAVal can be imposed by shift...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.130.2.635-641.1977

    authors: Ny T,Björk GR

    更新日期:1977-05-01 00:00:00

  • A dominant sulfhydryl-containing protein in the outer membrane of Neisseria gonorrhoeae.

    abstract::By using a method that labels sulfhydryl-containing proteins in situ, we have detected a major outer membrane protein of Neisseria gonorrhoeae at 41 kDa. A protein of this molecular mass has not previously been shown to be a major outer membrane protein in gonococci. In addition, a minor protein rich in cysteinyl resi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.4.1173-1175.1993

    authors: Norrod EP,Browne SL,Feldweg A,Leonard J

    更新日期:1993-02-01 00:00:00

  • Studies on Bd0934 and Bd3507, Two Secreted Nucleases from Bdellovibrio bacteriovorus, Reveal Sequential Release of Nucleases during the Predatory Cycle.

    abstract::Bdellovibrio bacteriovorus is an obligate predatory bacterium that invades and kills a broad range of Gram-negative prey cells, including human pathogens. Its potential therapeutic application has been the subject of increased research interest in recent years. However, an improved understanding of the fundamental mol...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00150-20

    authors: Bukowska-Faniband E,Andersson T,Lood R

    更新日期:2020-08-25 00:00:00

  • Glycosylation is required for outer membrane localization of the lectin LecB in Pseudomonas aeruginosa.

    abstract::The fucose-/mannose-specific lectin LecB from Pseudomonas aeruginosa is transported to the outer membrane; however, the mechanism used is not known so far. Here, we report that LecB is present in the periplasm of P. aeruginosa in two variants of different sizes. Both were functional and could be purified by their affi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01507-10

    authors: Bartels KM,Funken H,Knapp A,Brocker M,Bott M,Wilhelm S,Jaeger KE,Rosenau F

    更新日期:2011-03-01 00:00:00

  • Membrane chaperone SecDF plays a role in the secretion of Listeria monocytogenes major virulence factors.

    abstract::Listeria monocytogenes is a Gram-positive human intracellular pathogen that infects diverse mammalian cells. Upon invasion, L. monocytogenes secretes multiple virulence factors that target host cellular processes and promote infection. It has been presumed, but was not empirically established, that the Sec translocati...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00697-13

    authors: Burg-Golani T,Pozniak Y,Rabinovich L,Sigal N,Nir Paz R,Herskovits AA

    更新日期:2013-12-01 00:00:00

  • Immunochemistry of the pneumococcal types II, V, and VI. III. Tests with derivatives of the specific polysaccharides of types II and VI.

    abstract::Rebers, P. A. (Rutgers, The State University, New Brunswick, N. J.), E. Hurwitz, M. Heidelberger, and S. Estrada-Parra. Immunochemistry of the pneumococcal types II, V, and VI. III. Tests with derivatives of the specific polysaccharides of types II and VI. J. Bacteriol. 83:335-342. 1962.-The type-specific pneumococcal...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.2.335-342.1962

    authors: REBERS PA,HURWITZ E,HEIDELBERGER M,ESTRADA-PARRA S

    更新日期:1962-02-01 00:00:00

  • RelA inhibits Bacillus subtilis motility and chaining.

    abstract::The nucleotide second messengers pppGpp and ppGpp [(p)ppGpp] are responsible for the global downregulation of transcription, translation, DNA replication, and growth rate that occurs during the stringent response. More recent studies suggest that (p)ppGpp is also an important effector in many nonstringent processes, i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02063-14

    authors: Ababneh QO,Herman JK

    更新日期:2015-01-01 00:00:00

  • Neurospora crassa mutants deficient in asparagine synthetase.

    abstract::Neurospora crassa mutants deficient in asparagine synthetase were selected by using the procedure of inositol-less death. Complementation tests among the 100 mutants isolated suggested that their alterations were genetically allelic. Recombination analysis with strain S1007t, an asparagine auxotroph, indicated that th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.156.1.475-478.1983

    authors: MacPhee KG,Nelson RE,Schuster SM

    更新日期:1983-10-01 00:00:00

  • D-Allose catabolism of Escherichia coli: involvement of alsI and regulation of als regulon expression by allose and ribose.

    abstract::Genes involved in allose utilization of Escherichia coli K-12 are organized in at least two operons, alsRBACE and alsI, located next to each other on the chromosome but divergently transcribed. Mutants defective in alsI (allose 6-phosphate isomerase gene) and alsE (allulose 6-phosphate epimerase gene) were Als(-). Tra...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.22.7126-7130.1999

    authors: Poulsen TS,Chang YY,Hove-Jensen B

    更新日期:1999-11-01 00:00:00

  • Effect of pH and biotin on a circadian rhyythm of conidiation in Neurospora crassa.

    abstract::Expression of a circadian rhythm of conidiation in Neurospora crassa is enhanced in cultures grown on medium that is neutral or deficient in biotin. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.123.1.387-389.1975

    authors: West DJ

    更新日期:1975-07-01 00:00:00

  • Isolation and characterization of the Escherichia coli htrD gene, whose product is required for growth at high temperatures.

    abstract::Those genes in Escherichia coli defined by mutations which result in an inability to grow at high temperatures are designated htr, indicating a high temperature requirement. A new htr mutant of E. coli was isolated and characterized and is designated htrD. The htrD gene has been mapped to 19.3 min on the E. coli chrom...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.4.1240-1247.1992

    authors: Delaney JM,Ang D,Georgopoulos C

    更新日期:1992-02-01 00:00:00

  • Possible role of Escherichia coli penicillin-binding protein 6 in stabilization of stationary-phase peptidoglycan.

    abstract::Plasmids for high-level expression of penicillin-binding protein 6 (PBP6) were constructed, giving rise to overproduction of PBP6 under the control of the lambda pR promoter in either the periplasmic or the cytoplasmic space. In contrast to penicillin-binding protein 5 (PBP5), the presence of high amounts of PBP6 in t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.23.7572-7578.1992

    authors: van der Linden MP,de Haan L,Hoyer MA,Keck W

    更新日期:1992-12-01 00:00:00

  • Synthesis of the sigmaD protein is not sufficient to trigger expression of motility functions in Bacillus subtilis.

    abstract::The gene encoding sigmaD, sigD, is transcribed from two promoter regions, the fla/che promoter region in front of the fla/che operon and PsigD directly in front of sigD. If sigmaD is translated from transcripts originating from PsigD, the cell is unable to express motility functions but synthesizes autolysins. Therefo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.9.2942-2946.1999

    authors: Yang DH,von Kalckreuth J,Allmansberger R

    更新日期:1999-05-01 00:00:00

  • Function of the sigma(E) regulon in dead-cell lysis in stationary-phase Escherichia coli.

    abstract::Elevation of active sigma(E) levels in Escherichia coli by either repressing the expression of rseA encoding an anti-sigma(E) factor or cloning rpoE in a multicopy plasmid, led to a large decrease in the number of dead cells and the accumulation of cellular proteins in the medium in the stationary phase. The numbers o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.18.5231-5237.2000

    authors: Nitta T,Nagamitsu H,Murata M,Izu H,Yamada M

    更新日期:2000-09-01 00:00:00

  • Regulation of glutamine synthetase. VI. Interactions of inhibitors for Bacillus licheniformis glutamine synthetase.

    abstract::The relationships of five feedback inhibitors for the Bacillus licheniformis glutamine synthetase were investigated. The inhibitors were distinguishable by differences in their competitive relationship for the substrates of the enzyme. Mixtures of l-glutamine and adenosine-5'-monophosphate (AMP) or histidine and AMP c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.94.4.1016-1024.1967

    authors: Hubbard JS,Stadtman ER

    更新日期:1967-10-01 00:00:00

  • Draft genome sequence of Virgibacillus halodenitrificans 1806.

    abstract::Virgibacillus halodenitrificans 1806 is an endospore-forming halophilic bacterium isolated from salterns in Korea. Here, we report the draft genome sequence of V. halodenitrificans 1806, which may reveal the molecular basis of osmoadaptation and insights into carbon and anaerobic metabolism in moderate halophiles. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01280-12

    authors: Lee SJ,Lee YJ,Jeong H,Lee SJ,Lee HS,Pan JG,Kim BC,Lee DW

    更新日期:2012-11-01 00:00:00

  • tRNA Modification Profiles and Codon-Decoding Strategies in Methanocaldococcus jannaschii.

    abstract::tRNAs play a critical role in mRNA decoding, and posttranscriptional modifications within tRNAs drive decoding efficiency and accuracy. The types and positions of tRNA modifications in model bacteria have been extensively studied, and tRNA modifications in a few eukaryotic organisms have also been characterized and lo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00690-18

    authors: Yu N,Jora M,Solivio B,Thakur P,Acevedo-Rocha CG,Randau L,de Crécy-Lagard V,Addepalli B,Limbach PA

    更新日期:2019-04-09 00:00:00

  • Dual function of PilS during transcriptional activation of the Pseudomonas aeruginosa pilin subunit gene.

    abstract::The polar pili of Pseudomonas aeruginosa are composed of subunits encoded by the pilA gene. Expression of pilA requires the alternative sigma factor RpoN and a pair of regulatory elements, PilS and PilR. These two proteins are members of the two-component regulatory family, in which PilS is the sensory component and P...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.3.831-839.1996

    authors: Boyd JM,Lory S

    更新日期:1996-02-01 00:00:00

  • YycH and YycI interact to regulate the essential YycFG two-component system in Bacillus subtilis.

    abstract::The YycFG two-component system is the only signal transduction system in Bacillus subtilis known to be essential for cell viability. This system is highly conserved in low-G+C gram-positive bacteria, regulating important processes such as cell wall homeostasis, cell membrane integrity, and cell division. Four other ge...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01936-06

    authors: Szurmant H,Mohan MA,Imus PM,Hoch JA

    更新日期:2007-04-01 00:00:00

  • Characterization of the surface protein layers of the mosquito-pathogenic strains of Bacillus sphaericus.

    abstract::The protein surface layers on the cell walls of mosquito-pathogenic and nonpathogenic Bacillus sphaericus strains were studied by structural, biochemical, and serological methods. The surface structure of two representative insect-pathogenic strains had the form of a delicate linear array with a repeat interval of 5 n...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.1.72-79.1987

    authors: Lewis LO,Yousten AA,Murray RG

    更新日期:1987-01-01 00:00:00

  • Identification of YidC residues that define interactions with the Sec Apparatus.

    abstract::In bacteria, a subset of membrane proteins insert into the membrane via the Sec apparatus with the assistance of the widely conserved essential membrane protein insertase YidC. After threading into the SecYEG translocon, transmembrane segments of nascent proteins are thought to exit the translocon via a lateral gate i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01095-13

    authors: Li Z,Boyd D,Reindl M,Goldberg MB

    更新日期:2014-01-01 00:00:00

  • Cloning, nucleotide sequence, and overexpression of the gene coding for delta 5-3-ketosteroid isomerase from Pseudomonas putida biotype B.

    abstract::The structural gene coding for the delta 5-3-ketosteroid isomerase (KSI) of Pseudomonas putida biotype B has been cloned, and its entire nucleotide sequence has been determined by a dideoxynucleotide chain termination method. A 2.1-kb DNA fragment containing the ksi gene was cloned from a P. putida biotype B genomic l...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.21.6672-6676.1994

    authors: Kim SW,Kim CY,Benisek WF,Choi KY

    更新日期:1994-11-01 00:00:00

  • The pst operon of Bacillus subtilis has a phosphate-regulated promoter and is involved in phosphate transport but not in regulation of the pho regulon.

    abstract::Genes from Bacillus subtilis predicted to encode a phosphate-specific transport (Pst) system were shown by mutation to affect high-affinity Pi uptake but not arsenate resistance or phosphate (Pho) regulation. The transcription start of the promoter upstream of the pstS gene was defined by primer extension. The promote...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.8.2534-2539.1997

    authors: Qi Y,Kobayashi Y,Hulett FM

    更新日期:1997-04-01 00:00:00

  • Protein kinase/phosphatase function correlates with gliding motility in Mycoplasma pneumoniae.

    abstract::Mycoplasma pneumoniae exhibits a novel form of gliding motility that is mediated by the terminal organelle, a differentiated polar structure. Given that genes known to be involved in gliding in other organisms are absent in M. pneumoniae, random transposon mutagenesis was employed to generate mutants with gliding-defi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02277-12

    authors: Page CA,Krause DC

    更新日期:2013-04-01 00:00:00

  • Reconstitution of signaling in bacterial chemotaxis.

    abstract::Strains missing several genes required for chemotaxis toward amino acids, peptides, and certain sugars were tethered and their rotational behavior was analyzed. Null strains (called gutted) were deleted for genes that code for the transducers Tsr, Tar, Tap, and Trg and for the cytoplasmic proteins CheA, CheW, CheR, Ch...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.5.1878-1885.1987

    authors: Wolfe AJ,Conley MP,Kramer TJ,Berg HC

    更新日期:1987-05-01 00:00:00

  • Glucose-6-phosphate dehydrogenase from the chemolithotroph Thiobacillus ferrooxidans.

    abstract::Glucose-6-phosphate dehydrogenase was partially purified from both glucose-grown and iron-glucose-grown Thiobacillus ferrooxidans. The enzyme possesses a dual nucleotide specificity for either nicotinamide adenine dinucleotide phosphate (NADP) or nicotinamide adenine dinucleotide (NAD) and has a molecular weight of 11...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.108.1.343-352.1971

    authors: Tabita R,Lundgren DG

    更新日期:1971-10-01 00:00:00

  • Influence of genes encoding proton-translocating enzymes on suppression of Salmonella typhimurium growth and colonization.

    abstract::Twenty-four-hour-old, aerobically grown, Luria-Bertani broth cultures of Salmonella typhimurium F98 suppressed the growth of a spectinomycin-resistant (Spcr) derivative of the same strain inoculated at 10(3) CFU ml(-1). This growth suppression is genus specific and RpoS independent, and it is not solely a result of nu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.22.7186-7190.1997

    authors: Zhang-Barber L,Turner AK,Martin G,Frankel G,Dougan G,Barrow PA

    更新日期:1997-11-01 00:00:00

  • CovR and VicRKX Regulate Transcription of the Collagen Binding Protein Cnm of Streptococcus mutans.

    abstract::Cnm is a surface-associated protein present in a subset of Streptococcus mutans strains that mediates binding to extracellular matrices, intracellular invasion, and virulence. Here, we showed that cnm transcription is controlled by the global regulators CovR and VicRKX. In silico analysis identified multiple putative ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00141-18

    authors: Araújo Alves L,Ganguly T,Mattos-Graner RO,Kajfasz J,Harth-Chu EN,Lemos JA,Abranches J

    更新日期:2018-11-06 00:00:00

  • PilMNOPQ from the Pseudomonas aeruginosa type IV pilus system form a transenvelope protein interaction network that interacts with PilA.

    abstract::Pseudomonas aeruginosa type IV pili (T4P) are virulence factors that promote infection of cystic fibrosis and immunosuppressed patients. As the absence of T4P impairs colonization, they are attractive targets for the development of novel therapeutics. Genes in the pilMNOPQ operon are important for both T4P assembly an...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00032-13

    authors: Tammam S,Sampaleanu LM,Koo J,Manoharan K,Daubaras M,Burrows LL,Howell PL

    更新日期:2013-05-01 00:00:00