Abstract:
:Those genes in Escherichia coli defined by mutations which result in an inability to grow at high temperatures are designated htr, indicating a high temperature requirement. A new htr mutant of E. coli was isolated and characterized and is designated htrD. The htrD gene has been mapped to 19.3 min on the E. coli chromosome. Insertional inactivation of htrD with a mini-Tn10 element resulted in a pleiotropic phenotype characterized by a severe inhibition of growth at 42 degrees C and decreased survival at 50 degrees C in rich media. Furthermore, htrD cells were sensitive to H2O2. Growth rate analysis revealed that htrD cells grow very slowly in minimal media supplemented with amino acids. This inhibitory effect has been traced to the presence of cysteine in the growth medium. Further studies indicated that the rate of cysteine transport is higher in htrD cells relative to the wild type. All of these results, taken together, indicate that the htrD gene product may be required for proper regulation of intracellular cysteine levels and that an increased rate of cysteine transport greatly affects the growth characteristics of E. coli.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Delaney JM,Ang D,Georgopoulos Cdoi
10.1128/jb.174.4.1240-1247.1992keywords:
subject
Has Abstractpub_date
1992-02-01 00:00:00pages
1240-7issue
4eissn
0021-9193issn
1098-5530journal_volume
174pub_type
杂志文章abstract::Early log-phase cells of Mycoplasma gallisepticum A5969 were synchronized by holding in Eagle minimal essential medium (MEM) for 2 h. When transferred out of MEM into tryptose medium, the cells exhibited synchronous growth. Deoxyribonucleic acid (DNA) synthesis proceeded continuously during this growth but stopped dur...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.115.1.117-120.1973
更新日期:1973-07-01 00:00:00
abstract::Johnson, Russell C. (University of Minnesota, Minneapolis), and Palmer Rogers. Differentiation of pathogenic and saprophytic leptospires with 8-azaguanine. J. Bacteriol. 88:1618-1623. 1964.-The use of the purine analogue, 8-azaguanine, as a differential agent for the separation of pathogenic and saprophytic leptospire...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.6.1618-1623.1964
更新日期:1964-12-01 00:00:00
abstract::Phosphate import is required for the growth of mycobacteria and is regulated by environmental inorganic phosphate (P(i)) concentrations, although the mechanism of this regulation has not been characterized. The expression of genes involved in P(i) acquisition is frequently regulated by two-component regulatory systems...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00190-07
更新日期:2007-08-01 00:00:00
abstract::A homologue of the grmA spore germination gene of Bacillus megaterium and of a NaH-antiporter gene (napA) of Enterococcus hirae has been identified in Bacillus cereus 569 (ATCC 10876). The putative protein product has 58 and 43% amino acid identity with GrmA and NapA, respectively. Insertional inactivation of this B. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.2.476-482.2001
更新日期:2001-01-01 00:00:00
abstract::Twelve different dominant negative mutants of the Escherichia coli nucleoid-associated protein, H-NS, have been selected and characterized in vivo. The mutants are all severely defective in promoter repression activity in a strain lacking H-NS, and they all disrupt the repression normally exerted by H-NS at two of its...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.15.4335-4343.1996
更新日期:1996-08-01 00:00:00
abstract::Strains derived from HfrH carrying the arcA2 null mutation exhibit a higher respiratory rate, enhanced glucose consumption, and a more-reduced intracellular redox state than arcA deletion mutants of a different lineage. The phenotype of the arcA2 mutants was due to the presence of a creC constitutive mutation introduc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00040-08
更新日期:2008-05-01 00:00:00
abstract::The ugp operon of Escherichia coli includes genes involved in the uptake of sn-glycerol-3-phosphate and glycerophosphoryl diesters and belongs to the pho regulon which is induced by phosphate limitation. This operon has two transcriptional initiation sites, as determined by S1 nuclease mapping of the in vivo transcrip...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.2.549-558.1991
更新日期:1991-01-01 00:00:00
abstract::Oligoribonuclease, an exoribonuclease specific for small oligoribonucleotides, was initially characterized 20 years ago (S. K. Niyogi and A. K. Datta, J. Biol. Chem. 250:7307-7312, 1975) and shown to be different from RNase II and polynucleotide phosphorylase. Here we demonstrate, using mutant strains and purified enz...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.14.4137-4139.1995
更新日期:1995-07-01 00:00:00
abstract::Nahmias, André J. (Boston University School of Medicine, Boston, Mass.), and Sidney Kibrick. Inhibitory effect of heparin on herpes simplex virus. J. Bacteriol. 87:1060-1066. 1964.-A substance inhibitory to herpes simplex virus was observed during experiments with leukocyte cultures. The component in the cultures resp...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.87.5.1060-1066.1964
更新日期:1964-05-01 00:00:00
abstract::Recombinant Ralstonia eutropha strain PHB(-)4 expressing the broad-substrate-specificity polyhydroxyalkanoate (PHA) synthase 1 from Pseudomonas sp. strain 61-3 (PhaC1Ps) synthesizes a PHA copolymer containing the branched side-chain unit 3-hydroxy-4-methylvalerate (3H4MV), which has a carbon backbone identical to that...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.02474-14
更新日期:2015-04-01 00:00:00
abstract::Vibrio alginolyticus contained two adjacent genes, ktrA and ktrB, which encode a new type of bacterial K(+)-uptake system. KtrA and KtrB are peripheral and integral membrane proteins, respectively. Six of the nine sequenced bacterial genomes contain homologs to both ktrA and ktrB, suggesting that KtrAB is widespread. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.13.3491-3494.1998
更新日期:1998-07-01 00:00:00
abstract::Rhodospirillum rubrum was shown to grow fermentatively on fructose with N2 as a nitrogen source. The nitrogenase activity of these cells was regulated by the NH4+ switch-off/switch-on mechanism in a manner identical to that for photosynthetically grown cells. In vitro, the inactive nitrogenase Fe protein from fermenti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.162.3.1322-1324.1985
更新日期:1985-06-01 00:00:00
abstract::Livers of uninfected guinea pigs and of guinea pigs infected with Coxiella burneti were fractionated into smooth endoplasmic reticulum, rough endoplasmic reticulum (RER), pellet, and cell sap fractions. The ribonucleic acid (RNA) and protein of each fraction were determined, and the phosphorylase, glucose-6-phosphatas...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.102.2.334-340.1970
更新日期:1970-05-01 00:00:00
abstract::Glycerol trinitrate (GTN) reductase, which enables Agrobacterium radiobacter to utilize GTN and related explosives as sources of nitrogen for growth, was purified and characterized, and its gene was cloned and sequenced. The enzyme was a 39-kDa monomeric protein which catalyzed the NADH-dependent reductive scission of...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.24.7796-7802.1997
更新日期:1997-12-01 00:00:00
abstract::Two different mutations (pabA and pabB) affecting 4-aminobenzoate biosynthesis were obtained in strains of Escherichia coli lacking chorismate mutase and anthranilate synthetase activity, thus allowing study of the pathway of biosynthesis of 4-aminobenzoate by use of cell extracts of strains carrying the pab mutations...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.102.3.767-773.1970
更新日期:1970-06-01 00:00:00
abstract::Membrane fragments containing diacyl phospholipids were released from viable cells of Haemophilus parainfluenzae during incubation in ethylenediaminetetraacetic acid (EDTA)- tris(hydroxymethyl)aminomethane (Tris) buffer. The phospholipids located in the part of the membrane that was released during the EDTA-Tris treat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.103.2.329-334.1970
更新日期:1970-08-01 00:00:00
abstract::Glycerol:NAD+2-OXIDOREDUCTASE (EC 1.1.1.6) was purified to homogeneity from a mutant of Escherichia coli K12 that uses this enzyme, instead of ATP:glycerol 3-phosphotransferase (EC 2.7.1.30), as the first enzyme for the dissimilation of glycerol. Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sul...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.140.1.182-187.1979
更新日期:1979-10-01 00:00:00
abstract::Caldicellulosiruptor bescii efficiently degrades cellulose, xylan, and native grasses at high temperatures above 70°C under anaerobic conditions. C. bescii extracellularly secretes multidomain glycoside hydrolases along with proteins of unknown function. In this study, we analyzed the C. bescii proteins that bind to t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01897-14
更新日期:2014-11-01 00:00:00
abstract::Whole cells of Lactobacillus acidophilus are capable of degrading thymidine to thymine, suggesting the presence of thymidine phosphorylase (or thymidine hydrolase). This activity was also demonstrated in cell-free extracts. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.117.3.1358-1360.1974
更新日期:1974-03-01 00:00:00
abstract::Synchronized cultures of Anacystis nidulans (Synechococcus PCC 6301), an obligate phototroph, are obtained by incubating exponential cultures in the dark for 12 to 16 h. A temporal and sequential order of macromolecular synthesis is observed within the cell division cycle of a synchronously dividing culture in the lig...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.153.3.1315-1321.1983
更新日期:1983-03-01 00:00:00
abstract::Bdellovibrio bacteriovorus is an obligate predatory bacterium that invades and kills a broad range of Gram-negative prey cells, including human pathogens. Its potential therapeutic application has been the subject of increased research interest in recent years. However, an improved understanding of the fundamental mol...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00150-20
更新日期:2020-08-25 00:00:00
abstract::A mutant (pgi) of Coprinus macrorhizus deficient in phosphoglucose isomerase did not grow on fructose and grew poorly on glucose. The pgi mutation inhibited the formation of monokaryotic and dikaryotic fruiting bodies. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.144.1.432-434.1980
更新日期:1980-10-01 00:00:00
abstract::The soluble hydrogenase of Desulfovibrio vulgaris was purified and some of its properties are described. The molecular weight was determined for the enzyme by sedimentation equilibrium (45,400) and amino acid analysis (44,800). The hydrogenase appears to be a loosely coiled molecule or to have a high axial ratio, whic...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.1.249-258.1971
更新日期:1971-01-01 00:00:00
abstract::Deibel, R. H. (American Meat Institute Foundation, Chicago, Ill.), and J. H. Silliker. Food-poisoning potential of the enterococci. J. Bacteriol. 85:827-832. 1963.-A total of 23 enterococcus strains were fed to two and sometimes three human volunteers in an effort to elicit food-poisoning symptoms. Each culture was co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.85.4.827-832.1963
更新日期:1963-04-01 00:00:00
abstract::Morello, Josephine A. (Boston University School of Medicine, Boston, Mass.), Teresa A. DiGenio, and Edgar E. Baker. Significance of salmonellae isolated from apparently healthy mice. J. Bacteriol. 89:1460-1464. 1965.-Three species of Salmonella were isolated from three groups of apparently healthy laboratory mice. The...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.6.1460-1464.1965
更新日期:1965-06-01 00:00:00
abstract::Smith, Paul F. (University of Pennsylvania, Philadelphia) and George H. Rothblat. Comparison of pleuropneumonia-like and L-type organisms. J. Bacteriol. 83:500-506. 1962.-The content of total lipid and nonsaponifiable lipid depended upon the type of organism; the greatest amounts were found in pleuropneumonia-like org...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.3.500-506.1962
更新日期:1962-03-01 00:00:00
abstract::Transferable plasmids play an important role in the dissemination of clindamycin-erythromycin resistance in Bacteroides fragilis. We previously described the isolation and properties of pBFTM10, a 14.9-kb ClnR transfer factor from B. fragilis TMP10. We also reported the isolation of a transfer-deficient deletion deriv...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.23.7471-7480.1991
更新日期:1991-12-01 00:00:00
abstract::Analysis of a region involved in the conjugative transfer of the lactococcal conjugative element pRS01 has revealed a bacteria] group II intron. Splicing of this lactococcal intron (designated Ll.ltrB) in vivo resulted in the ligation of two exon messages (ltrBE1 and ltrBE2) which encoded a putative conjugative relaxa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.12.3531-3538.1996
更新日期:1996-06-01 00:00:00
abstract::The Aer protein in Escherichia coli is a membrane-bound, FAD-containing aerotaxis and energy sensor that putatively monitors the redox state of the electron transport system. Binding of FAD to Aer requires the N-terminal PAS domain and residues in the F1 region and C-terminal HAMP domain. The PAS domains of other PAS ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.20.6782-6791.2004
更新日期:2004-10-01 00:00:00
abstract::The luminescence (lux) operon (luxICDABEG) of the symbiotic bacterium Vibrio fischeri is regulated by the transcriptional activator LuxR and two acyl-homoserine lactone (acyl-HSL) autoinducers (the luxI-dependent 3-oxo-hexanoyl-HSL [3-oxo-C6-HSL] and the ainS-dependent octanoyl-HSL [C8-HSL]) in a population density-re...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.10.2811-2822.2000
更新日期:2000-05-01 00:00:00