Isolation and characterization of the Escherichia coli htrD gene, whose product is required for growth at high temperatures.

Abstract:

:Those genes in Escherichia coli defined by mutations which result in an inability to grow at high temperatures are designated htr, indicating a high temperature requirement. A new htr mutant of E. coli was isolated and characterized and is designated htrD. The htrD gene has been mapped to 19.3 min on the E. coli chromosome. Insertional inactivation of htrD with a mini-Tn10 element resulted in a pleiotropic phenotype characterized by a severe inhibition of growth at 42 degrees C and decreased survival at 50 degrees C in rich media. Furthermore, htrD cells were sensitive to H2O2. Growth rate analysis revealed that htrD cells grow very slowly in minimal media supplemented with amino acids. This inhibitory effect has been traced to the presence of cysteine in the growth medium. Further studies indicated that the rate of cysteine transport is higher in htrD cells relative to the wild type. All of these results, taken together, indicate that the htrD gene product may be required for proper regulation of intracellular cysteine levels and that an increased rate of cysteine transport greatly affects the growth characteristics of E. coli.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Delaney JM,Ang D,Georgopoulos C

doi

10.1128/jb.174.4.1240-1247.1992

keywords:

subject

Has Abstract

pub_date

1992-02-01 00:00:00

pages

1240-7

issue

4

eissn

0021-9193

issn

1098-5530

journal_volume

174

pub_type

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