Purification and properties of a hydrogenase from Desulfovibrio vulgaris.

abstract：:We have devised a coupled radiochemical assay for detecting phosphatidylglycerolphosphate (PGP) phosphatase activity in Escherichia coli colonies immobilized on filter paper. There appeared to be at least two enzymes capable of dephosphorylating PGP, as judged by the characterization of mutations in two genes designat...

journal_title：Journal of bacteriology

authors： Icho T,Raetz CR

更新日期：1983-02-01 00:00:00

abstract：:Acidithiobacillus caldus is an extremely acidophilic, moderately thermophilic, chemolithoautotrophic gammaproteobacterium that derives energy from the oxidation of sulfur and reduced inorganic sulfur compounds. Here we present the draft genome sequence of Acidithiobacillus caldus ATCC 51756 (the type strain of the spe...

journal_title：Journal of bacteriology

authors： Valdes J,Quatrini R,Hallberg K,Dopson M,Valenzuela PD,Holmes DS

更新日期：2009-09-01 00:00:00

abstract：:We have identified the transcription start site and regulatory region governing the expression of a sporulation gene in the spoIVC locus of Bacillus subtilis. Efficient expression and developmental regulation of this gene was controlled from a promoter region that extended no more than 110 base pairs upstream and no m...

journal_title：Journal of bacteriology

authors： Kunkel B,Sandman K,Panzer S,Youngman P,Losick R

更新日期：1988-08-01 00:00:00

abstract：:The N-terminal sequence of a protein, originally described as an adhesin of Helicobacter pylori, was used in an oligonucleotide-based screening procedure of an H. pylori plasmid library in Escherichia coli. Five independent plasmid clones were isolated, all mapping to the same chromosomal region and encoding the H. py...

journal_title：Journal of bacteriology

authors： Odenbreit S,Wieland B,Haas R

更新日期：1996-12-01 00:00:00

abstract：:The potassium permeability of Rickettsia prowazekii was characterized by chemical measurement of the intracellular sodium and potassium pools and isotopic flux measurements with 86Rb+ as a tracer. R. prowazekii, in contrast to Escherichia coli, did not maintain a high potassium-to-sodium ratio in their cytoplasm excep...

journal_title：Journal of bacteriology

authors： Winkler HH

更新日期：1984-01-01 00:00:00

abstract：:Microtubular and lamellate membrane structures were observed at the log phase of growth in the stable L-forms of Escherichia coli cultured in the absence of penicillin. ...

journal_title：Journal of bacteriology

authors： Eda T,Kanda Y,Kimura S

更新日期：1976-09-01 00:00:00

abstract：:In this study, we demonstrated the remarkable genome plasticity of lytic lactococcal phages that allows them to rapidly adapt to the dynamic dairy environment. The lytic double-stranded DNA phage ul36 was used to sequentially infect a wild-type strain of Lactococcus lactis and two isogenic derivatives with genes encod...

journal_title：Journal of bacteriology

authors： Labrie SJ,Moineau S

更新日期：2007-02-01 00:00:00

abstract：:The genes for biosynthesis of the branched-chain amino acids leucine, isoleucine, and valine in Lactococcus lactis subsp. lactis NCDO2118 were characterized by cloning, complementation in Escherichia coli and Bacillus subtilis, and nucleotide sequence analysis. Nine structural genes are clustered on a 12-kb DNA fragme...

journal_title：Journal of bacteriology

authors： Godon JJ,Chopin MC,Ehrlich SD

更新日期：1992-10-01 00:00:00

abstract：:The structure of sheathed flagella from Bdellovibrio bacteriovorus was investigated. The first three periods of these flagella were characterized by progressively smaller wavelengths and amplitudes in periods more distal to the cell. The damped appearance was due to a single nonrandom transition between two helical st...

journal_title：Journal of bacteriology

authors： Thomashow LS,Rittenberg SC

更新日期：1985-09-01 00:00:00

abstract：:MinD binds to phospholipid vesicles in the presence of ATP and is released by MinE, which stimulates the MinD ATPase. Membrane binding requires a short conserved C-terminal region, which has the potential to form an amphipathic helix. This finding has led to a model in which the binding of ATP regulates the formation ...

journal_title：Journal of bacteriology

authors： Zhou H,Lutkenhaus J

更新日期：2003-08-01 00:00:00

abstract：:Harper, M. K. (U.S. Department of Agriculture, Washington, D.C.), H. R. Curran, and M. J. Pallansch. Properties of electrodialyzed bacterial spores. J. Bacteriol. 88:1338-1340. 1964.-Washed spores of Bacillus cereus, B. megaterium, and B. stearothermophilis suspended in distilled water were electrodialyzed at a potent...

journal_title：Journal of bacteriology

authors： HARPER MK,CURRAN HR,PALLANSCH MJ

更新日期：1964-11-01 00:00:00

abstract：:Species of marine bacteria belonging to the genus Beneckea and strains of Photobacterium fischeri were negatively stained and examined by means of the electron microscope to determine the structure and arrangement of their flagella. All of the species of the genus Beneckea had single, polar, sheathed flagella when gro...

journal_title：Journal of bacteriology

authors： Allen RD,Baumann P

更新日期：1971-07-01 00:00:00

abstract：:The crystal structure of a small, basic DNA binding protein, Sso10b2, from the thermoacidophilic archaeon Sulfolobus solfataricus was determined by the Zn multiwavelength anomalous diffraction method and refined to 1.85 A resolution. The 89-amino-acid protein adopts a betaalphabetaalphabetabeta topology. The structure...

journal_title：Journal of bacteriology

authors： Chou CC,Lin TW,Chen CY,Wang AH

更新日期：2003-07-01 00:00:00

abstract：:A characteristic of N2-fixing cyanobacteria in symbiotic associations appears to be release of N2-derived NH4+. The specific activity of the primary ammonium-assimilating enzyme, glutamine synthetase (GS), was found to be three- to fourfold lower in Nostoc sp. strain 7801 grown in symbiotic association with the bryoph...

journal_title：Journal of bacteriology

authors： Joseph CM,Meeks JC

更新日期：1987-06-01 00:00:00

abstract：:Those genes in Escherichia coli defined by mutations which result in an inability to grow at high temperatures are designated htr, indicating a high temperature requirement. A new htr mutant of E. coli was isolated and characterized and is designated htrD. The htrD gene has been mapped to 19.3 min on the E. coli chrom...

journal_title：Journal of bacteriology

authors： Delaney JM,Ang D,Georgopoulos C

更新日期：1992-02-01 00:00:00

abstract：:The effect of L-methionine-DL-sulfoximine, an inhibitor of glutamine synthetase, on the formation of nitrate reductase in the wild-type strain of Neurospora in the presence of ammonium ions and of glutamine was studied. Under conditions in which glutamine synthetase was inactivated, it was found that only glutamine co...

journal_title：Journal of bacteriology

authors： Premakumar R,Sorger GJ,Gooden D

更新日期：1980-07-01 00:00:00

abstract：:Mu promoters active during the lytic cycle were located by isolating RNA at various times after induction of Mu prophages, radiolabeling it by capping in vitro, and hybridizing it to Mu DNA fragments on Southern blots. Signals were detected from four new promoters in addition to the previously characterized Pe (early)...

journal_title：Journal of bacteriology

authors： Stoddard SF,Howe MM

更新日期：1989-06-01 00:00:00

abstract：:The Klebsiella aerogenes hutUH operon is preceded by a promoter region, hut(P), that contains two divergent promoters (hutUp and Pc) which overlap and are alternately expressed. In the absence of the catabolite gene activator protein-cyclic AMP (CAP-cAMP) complex, Pc is predominantly expressed while hutUp is largely r...

journal_title：Journal of bacteriology

authors： Osuna R,Janes BK,Bender RA

更新日期：1994-09-01 00:00:00

abstract：:Type 3 fimbriae are adhesive organelles found in enterobacterial pathogens. The fimbriae promote biofilm formation on biotic and abiotic surfaces; however, the exact identity of the receptor for the type 3 fimbriae adhesin, MrkD, remains elusive. We analyzed naturally occurring structural and functional variabilities ...

journal_title：Journal of bacteriology

authors： Stahlhut SG,Chattopadhyay S,Kisiela DI,Hvidtfeldt K,Clegg S,Struve C,Sokurenko EV,Krogfelt KA

更新日期：2013-12-01 00:00:00

abstract：:The group A Streptococcus (GAS) sof gene encodes the serum opacity factor protein, which is capable of opacifying mammalian sera and binding at least two host proteins, fibronectin and fibrinogen. The sof gene exists in approximately 50% of clinical isolates, and there is a classical association of so-called nephritog...

journal_title：Journal of bacteriology

authors： Jeng A,Sakota V,Li Z,Datta V,Beall B,Nizet V

更新日期：2003-02-01 00:00:00

abstract：:A mutant of Escherichia coli lacking hydroxyethylthiazole kinase (EC 2.7.1.50) was produced by a further mutation of a temperature-sensitive, auxotrophic mutant for hydroxyethylthiazole. The parent cells possessed two distinct enzymes capable of phosphorylating hydroxyethylthiazole: one was hydroxyethylthiazole kinase...

journal_title：Journal of bacteriology

authors： Mizote T,Nakayama H

更新日期：1989-06-01 00:00:00

abstract：:Induction of nod genes in Rhizobium and Bradyrhizobium species is dependent on the presence of plant-produced flavonoids, the NodD protein, and the cis-acting nod box promoter sequence. Although the nodD (nodD1) gene in Rhizobium species is constitutively expressed, nodD1 expression in Bradyrhizobium japonicum is indu...

journal_title：Journal of bacteriology

authors： Wang SP,Stacey G

更新日期：1991-06-01 00:00:00

abstract：:Pseudomonas fluorescens strain KU-7 is a prototype microorganism that metabolizes 2-nitrobenzoate (2-NBA) via the formation of 3-hydroxyanthranilate (3-HAA), a known antioxidant and reductant. The initial two steps leading to the sequential formation of 2-hydroxy/aminobenzoate and 3-HAA are catalyzed by a NADPH-depend...

journal_title：Journal of bacteriology

authors： Iwaki H,Muraki T,Ishihara S,Hasegawa Y,Rankin KN,Sulea T,Boyd J,Lau PC

更新日期：2007-05-01 00:00:00

abstract：:We have identified a gene encoding a putative membrane protein homologous to the major facilitator superfamily, mapping upstream of the lacS gene in Sulfolobus solfataricus. Permeases from this family mediate secondary transport and are widely distributed among eubacteria and eukaryotes; the finding of an archaeal mem...

journal_title：Journal of bacteriology

authors： Prisco A,Moracci M,Rossi M,Ciaramella M

更新日期：1995-03-01 00:00:00

abstract：:Nagai, Susumu (National Women's University, Nara, Japan). Differential reduction of tellurite by growing colonies of normal yeast and respiration-deficient mutants. J. Bacteriol. 90:220-222. 1965.-A differential reduction of sodium tellurite was observed between normal and respiration-deficient mutant colonies of seve...

journal_title：Journal of bacteriology

authors： Nagai S

更新日期：1965-07-01 00:00:00

abstract：:Form I ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) of the Calvin-Benson-Bassham cycle may be divided into two broad phylogenetic groups, referred to as red-like and green-like, based on deduced large subunit amino acid sequences. Unlike the form I enzyme from the closely related organism Rhodobacter spha...

journal_title：Journal of bacteriology

authors： Horken KM,Tabita FR

更新日期：1999-07-01 00:00:00

abstract：:The region upstream of the Escherichia coli bgl operon is an insertion hot spot for several transposons. Elements as distantly related as Tn1, Tn5, and phage Mu home in on this location. To see what characteristics result in a high-affinity site for transposition, we compared in vivo and in vitro Mu transposition patt...

journal_title：Journal of bacteriology

authors： Manna D,Wang X,Higgins NP

更新日期：2001-06-01 00:00:00

abstract：:Catabolism of nonphenolic arenes is frequently initiated by dioxygenases, yielding single isomer products with two adjacent hydroxylated asymmetric centers. The next enzymic reaction dehydrogenates these cyclic cis-diols, with aromatization yielding catechols for ring cleavage. There are two stereochemical questions t...

journal_title：Journal of bacteriology

authors： Morawski B,Casy G,Illaszewicz C,Griengl H,Ribbons DW

更新日期：1997-06-01 00:00:00

abstract：:Strains carrying either the polA1 or resA1 mutation are deficient in DNA polymerase I, and the polA1 and resA1 mutations do not complement in merozygotes. The effect of these mutations in otherwise identical genetic backgrounds was studied: after ultraviolet irradiation both strains degrade their DNA more rapidly and ...

journal_title：Journal of bacteriology

authors： Glickman BW,van Sluis CA,van der Maas G,Rörsch A

更新日期：1973-06-01 00:00:00

abstract：:The regulatory properties of acetohydroxy acid synthetase (AHAS), the first enzyme in the biosynthetic pathway to valine and the second in the isoleucine pathway, were investigated in the fission yeast Schizosaccharomyces pombe. The enzyme was partially purified from crude extracts by protamine sulfate treatment, ammo...

journal_title：Journal of bacteriology

authors： McDonald RA,Satyanarayana T,Kaplan JG

更新日期：1973-04-01 00:00:00