Abstract:
:The structure of sheathed flagella from Bdellovibrio bacteriovorus was investigated. The first three periods of these flagella were characterized by progressively smaller wavelengths and amplitudes in periods more distal to the cell. The damped appearance was due to a single nonrandom transition between two helical structures within each filament. The intersection of the two helices, one of which was a threefold-reduced miniature of the other, occurred at a fixed distance along the filament and resulted in a shift in the flagellar axis. Flagella increased in length as the cells aged and assumed a constant miniature waveform at their distal ends. The core filament was the principal determinant of flagellar morphology. It was composed of 28,000- and 29,500-dalton polypeptides. The 28,000-dalton subunits were located in the cell-proximal segment of the filament, and the 29,500-dalton subunits were located in the more distal region. The heteromorphous appearance of bdellovibrio flagella arose from the sequential assembly of these subunits. The basal complex associated with core filaments was examined because of its potential involvement in sheath formation. Bdellovibrio basal organelles were generally similar to those of other gram-negative species, but appeared to lack a disk analogous to the outer membrane-associated L ring which is a normal component of gram-negative basal complexes.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Thomashow LS,Rittenberg SCdoi
10.1128/JB.163.3.1038-1046.1985subject
Has Abstractpub_date
1985-09-01 00:00:00pages
1038-46issue
3eissn
0021-9193issn
1098-5530journal_volume
163pub_type
杂志文章abstract::Helicobacter pylori is a human gastric pathogen infecting almost half of the world population. Herein, we present an updated version of the metabolic reconstruction of H. pylori strain 26695 based on the revised genome annotation and new experimental data. This reconstruction, iIT341 GSM/GPR, represents a detailed rev...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.16.5818-5830.2005
更新日期:2005-08-01 00:00:00
abstract::In gram-negative organisms, enzymes belonging to the low-molecular-weight protein tyrosine phosphatase (LMPTP) family are involved in the regulation of important physiological functions, including stress resistance and synthesis of the polysaccharide capsule. LMPTPs have been identified also in gram-positive bacteria,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.14.4945-4956.2005
更新日期:2005-07-01 00:00:00
abstract::Site-specific mutagenesis has identified amino acids involved in bR proton transport. Biophysical studies of the mutants have elucidated the roles of two membrane-embedded residues: Asp-85 serves as the acceptor for the proton from the isomerized retinylidene Schiff base, and Asp-96 participates in reprotonation of th...
journal_title:Journal of bacteriology
pub_type: 杂志文章,评审
doi:10.1128/jb.175.6.1555-1560.1993
更新日期:1993-03-01 00:00:00
abstract::Galactose-negative mutants of the group H Streptococcus strain Challis were obtained by treatment with nitrosoguanidine. Enzyme assays of extracts of these mutants revealed that 12 of the mutants were lacking one of the enzymes of the Leloir pathway. Thus, the Leloir pathway is the major means of galactose metabolism ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.109.2.512-519.1972
更新日期:1972-02-01 00:00:00
abstract::The reduction of bisulfite by Desulfovibrio vulgaris was investigated. Crude extracts reduced bisulfite to sulfide without the formation (detection) of any intermediates such as trithionate or thiosulfate. When the particulate fractions was removed from crude extracts by high-speed centrifugation, the soluble supernat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.136.3.916-923.1978
更新日期:1978-12-01 00:00:00
abstract::The expression of Escherichia coli purR, which encodes the pur regulon repressor protein, is autoregulated. Autoregulation at the level of transcription requires two operator sites, designated purRo1 and purRo2 (O1 and O2). Operator O1 is in the region of DNA between the transcription start site and the site for trans...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.10.5758-5766.1990
更新日期:1990-10-01 00:00:00
abstract::Inhibition of sterol uptake in Saccharomyces cerevisiae sterol auxotroph FY3 (alpha hem1 erg7 ura) by delta-aminolevulinic acid (ALA) is dependent on the ability of the organism to synthesize heme from ALA. Sterol-depleted cells not exposed to ALA or strain PFY3 cells, with a double heme mutation, exposed to ALA did n...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.8.3707-3711.1987
更新日期:1987-08-01 00:00:00
abstract::Expression of the Bacillus subtilis trpEDCFBA operon has been shown to be regulated by transcription attenuation in response to the availability of L-tryptophan. Regulation is mediated by the tryptophan-activated trp RNA-binding attenuation protein, TRAP, the product of mtrB. Formation of mutually exclusive RNA anti-t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.22.6362-6370.1995
更新日期:1995-11-01 00:00:00
abstract::A cloned 8.3-kilobase-pair DNA fragment carrying all the genes (iucABCD iutA) of the aerobactin iron transport system of plasmid pColV-K30 was subjected to in vitro mutagenesis to afford mutant genes iucA, iucC, and iucA iucC. Complementation analyses and identification of aerobactin precursors accumulated by Escheric...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.167.1.350-355.1986
更新日期:1986-07-01 00:00:00
abstract::Relatively high transfo mation frequencies were obtained with Micrococcus lysodeikticus cells, previou[ill]sly grown in defined broth, with a tris(hydroxymethyl)-aminomethane-glutamate b uffer containing Mg(++), Ca(++), Ba(++), or Sr(++). ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.98.3.1397-1399.1969
更新日期:1969-06-01 00:00:00
abstract::Arthrobacter sp. strain TAD20, a chitinolytic gram-positive organism, was isolated from the sea bottom along the Antarctic ice shell. Arthrobacter sp. strain TAD20 secretes two major chitinases, ChiA and ChiB (ArChiA and ArChiB), in response to chitin induction. A single chromosomal DNA fragment containing the genes c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.5.1773-1779.2001
更新日期:2001-03-01 00:00:00
abstract::The aerobic respiratory chain of Escherichia coli can function with either of two different membrane-bound NADH dehydrogenases (NDH-1 and NDH-2) and with either of two ubiquinol oxidases (bd-type and bo-type). The amounts of each of these enzymes present in the E. coli membrane depend on growth conditions in general a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.10.3020-3025.1993
更新日期:1993-05-01 00:00:00
abstract::Addition of urea to an uninduced culture of Saccharomyces at 22 C results in appearance of allophanate hydrolase activity after a lag of 12 min. We have previously demonstrated that both ribonucleic acid (RNA) and protein synthesis are needed for this induction to occur. To elucidate the time intervals occupied by kno...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.126.1.198-204.1976
更新日期:1976-04-01 00:00:00
abstract::The physiological changes in an F1-ATPase-defective mutant of Escherichia coli W1485 growing in a glucose-limited chemostat included a decreased growth yield (60%) and increased specific rates of both glucose consumption (168%) and respiration (171%). Flux analysis revealed that the mutant showed approximately twice a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00452-06
更新日期:2006-10-01 00:00:00
abstract::During development of the symbiotic soil bacterium Sinorhizobium meliloti into nitrogen-fixing bacteroids, DNA replication and cell division cease and the cells undergo profound metabolic and morphological changes. Regulatory genes controlling the early stages of this process have not been identified. As a first step ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.10.3204-3210.2001
更新日期:2001-05-01 00:00:00
abstract::Using mixed-species cultures, we have undertaken a study of interactions between two common spore-forming soil bacteria, Bacillus subtilis and Streptomyces coelicolor. Our experiments demonstrate that the development of aerial hyphae and spores by S. coelicolor is inhibited by surfactin, a lipopeptide surfactant produ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00162-06
更新日期:2006-07-01 00:00:00
abstract::We report a draft sequence of the genome of Gordonia neofelifaecis NRRL B-59395, a cholesterol-degrading actinomycete isolated from fresh feces of a clouded leopard (Neofelis nebulosa). As predicted, the reported genome contains several gene clusters for cholesterol degradation. This is the second available genome seq...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05531-11
更新日期:2011-09-01 00:00:00
abstract::DNA damage-inducible DNA replication in SOS-induced Escherichia coli cells, termed inducible stable DNA replication (iSDR), has previously been shown to require either the RecBCD or the RecE pathway of homologous recombination for initiation. Here, we demonstrate that recB recC sbcC quadruple mutant cells are capable ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.22.7113-7114.1994
更新日期:1994-11-01 00:00:00
abstract::A lipoteichoic acid (LTA) was extracted from Listeria monocytogenes (serotype 1) by phenol-water partition and isolated by gel-filtration chromatography. The LTA exhibited amphiphilic properties by changes in gel-filtration mobility in the presence of detergent buffers and after mild base hydrolysis. In a hemagglutina...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.156.2.809-817.1983
更新日期:1983-11-01 00:00:00
abstract::A marked difference was found to exist between the nicotinamide adenine dinucleotide (NAD) glycohydrolase activity of human strains of Mycobacterium tuberculosis as compared with bovine strains. Human strains had from 6- to 20-fold higher NAD glycohydrolase activity than bovine strains. This finding explains the accum...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.110.2.600-603.1972
更新日期:1972-05-01 00:00:00
abstract::Bacillus subtilis, which grows under aerobic conditions, employs fatty acid desaturase (Des) to fluidize its membrane when subjected to temperature downshift. Des requires molecular oxygen for its activity, and its expression is regulated by DesK-DesR, a two-component system. Transcription of des is induced by the tem...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00384-10
更新日期:2010-08-01 00:00:00
abstract::Two novel insertion sequences, IS1312 and IS1313, were found in pTiBo542, the Ti plasmid of Agrobacterium tumefaciens strains Bo542 and A281. Nucleotide sequencing and Southern hybridization revealed that IS1312 and IS1313 are homologous to Rhizobium meliloti ISRm1 and ISRm2, respectively. IS1312, ISRm1, and another A...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.9.2554-2559.1995
更新日期:1995-05-01 00:00:00
abstract::A mutant (designated mec(-)) of Escherichia coli F(+) 100 endo I(-)su(+) r(K) (-)m(K) (+) has been isolated which is defective in cytosine-specific deoxyribonucleic acid (DNA) methylase activity. The DNA of this mutant, as well as the DNA of phages lambda and fd propagated in it, is virtually devoid of 5-methyl-cytosi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.115.3.1103-1107.1973
更新日期:1973-09-01 00:00:00
abstract::Filter matings between E. coli K-12 strains carrying an F'::Tn5,Tn9 factor with H. influenzae Rd strains gave rise to kanamycin-chloramphenicol-resistant H. influenzae strains at a frequency of approximately 10(-6). Transfer of the F' factor to H. influenzae was verified by expression of unselected markers in H. influ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.5.1905-1910.1987
更新日期:1987-05-01 00:00:00
abstract::LamB-LacZ fusion proteins have classically been used in studies of the general secretion pathway of Escherichia coli. Here we describe how increasing signal sequence hydrophobicity routes LamB-LacZ Hyb42-1 to the signal recognition particle (SRP) pathway. Secretion of this hydrophobic fusion variant (H*LamB-LacZ) was ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.19.5697-5705.2003
更新日期:2003-10-01 00:00:00
abstract::Exoelectrogens are able to transfer electrons extracellularly, enabling them to respire on insoluble terminal electron acceptors. Extensively studied exoelectrogens, such as Geobacter sulfurreducens and Shewanella oneidensis, are Gram negative. More recently, it has been reported that Gram-positive bacteria, such as L...
journal_title:Journal of bacteriology
pub_type: 评论,杂志文章
doi:10.1128/JB.00029-20
更新日期:2020-03-11 00:00:00
abstract::Expression of the Escherichia coli leuV operon, which contains three tRNA(1)(Leu) genes, is regulated by several mechanisms including growth-rate-dependent control (GRDC) and stringent control (SC). Structural variants of the leuV promoter which differentially affect these regulatory responses have been identified, su...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.18.5771-5782.1999
更新日期:1999-09-01 00:00:00
abstract::Diaminobenzidine oxidation product occurred in peroxisomes and in the intracristate spaces of mitochondria. The reaction was inhibited only in peroxisomes when 3-amino-1,2,4-triazole was present, but cyanide and azide inhibited deposition in both kinds of organelles. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.104.1.581-584.1970
更新日期:1970-10-01 00:00:00
abstract::31P and 13C nuclear magnetic resonance (NMR) experiments were performed on suspensions of the phototrophic bacterium Chromatium vinosum incubated anaerobically in the dark. 31P NMR spectra revealed that during prolonged dark incubation high ATP levels are maintained. This phenomenon was independent of the presence of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.155.2.634-642.1983
更新日期:1983-08-01 00:00:00
abstract::In Escherichia coli K-12, expression of the lysU gene is regulated by the lrp gene product, as indicated by an increase in the level of lysyl-tRNA synthetase activity and LysU protein in an lrp mutant. Comparison of the patterns of protein expression visualized by two-dimensional gel electrophoresis indicated that Lys...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.9.2779-2784.1992
更新日期:1992-05-01 00:00:00