Differences in cold adaptation of Bacillus subtilis under anaerobic and aerobic conditions.

Abstract:

:Bacillus subtilis, which grows under aerobic conditions, employs fatty acid desaturase (Des) to fluidize its membrane when subjected to temperature downshift. Des requires molecular oxygen for its activity, and its expression is regulated by DesK-DesR, a two-component system. Transcription of des is induced by the temperature downshift and is decreased when membrane fluidity is restored. B. subtilis is also capable of anaerobic growth by nitrate or nitrite respiration. We studied the mechanism of cold adaptation in B. subtilis under anaerobic conditions that were predicted to inhibit Des activity. We found that in anaerobiosis, in contrast to aerobic growth, the induction of des expression after temperature downshift (from 37 degrees C to 25 degrees C) was not downregulated. However, the transfer from anaerobic to aerobic conditions rapidly restored the downregulation. Under both aerobic and anaerobic conditions, the induction of des expression was substantially reduced by the addition of external fluidizing oleic acid and was fully dependent on the DesK-DesR two-component regulatory system. Fatty acid analysis proved that there was no desaturation after des induction under anaerobic conditions despite the presence of high levels of the des protein product, which was shown by immunoblot analysis. The cold adaptation of B. subtilis in anaerobiosis is therefore mediated exclusively by the increased anteiso/iso ratio of branched-chain fatty acids and not by the temporarily increased level of unsaturated fatty acids that is typical under aerobic conditions. The degrees of membrane fluidization, as measured by diphenylhexatriene fluorescence anisotropy, were found to be similar under both aerobic and anaerobic conditions.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Beranová J,Mansilla MC,de Mendoza D,Elhottová D,Konopásek I

doi

10.1128/JB.00384-10

subject

Has Abstract

pub_date

2010-08-01 00:00:00

pages

4164-71

issue

16

eissn

0021-9193

issn

1098-5530

pii

JB.00384-10

journal_volume

192

pub_type

杂志文章
  • Role of lithium ions in proline transport in Escherichia coli.

    abstract::Mechanisms of Li+ stimulation of proline transport were studied in cells of Escherichia coli 7 and NR70, a mutant of strain 7 lacking adenosine triphosphatase (EC 3.6.1.3). An electrochemical potential difference of Li+ induced in an inward direction of energy-depleted cells caused a transient uptake of proline depend...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.139.2.560-564.1979

    authors: Kayama-Gonda Y,Kawasaki T

    更新日期:1979-08-01 00:00:00

  • Intermediate location in the assembly of the matrix protein or porin into the outer membrane of Escherichia coli.

    abstract::Evidence from pulse-chase experiments indicates that the outer membrane matrix protein or porin of Escherichia coli B/r passes through a Sarkosyl-soluble membrane pool on the way to its eventual Sarkosyl-insoluble state in the outer membrane. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.143.3.1538-1541.1980

    authors: Boyd A,Holland IB

    更新日期:1980-09-01 00:00:00

  • Purification and characterization of two extremely thermostable enzymes, phosphate acetyltransferase and acetate kinase, from the hyperthermophilic eubacterium Thermotoga maritima.

    abstract::Phosphate acetyltransferase (PTA) and acetate kinase (AK) of the hyperthermophilic eubacterium Thermotoga maritima have been purified 1,500- and 250-fold, respectively, to apparent homogeneity. PTA had an apparent molecular mass of 170 kDa and was composed of one subunit with a molecular mass of 34 kDa, suggesting a h...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.6.1861-1867.1999

    authors: Bock AK,Glasemacher J,Schmidt R,Schönheit P

    更新日期:1999-03-01 00:00:00

  • Genetic Basis Underlying the Hyperhemolytic Phenotype of Streptococcus agalactiae Strain CNCTC10/84.

    abstract::Streptococcus agalactiae (group B streptococcus [GBS]) is a major cause of infections in newborns, pregnant women, and immunocompromised patients. GBS strain CNCTC10/84 is a clinical isolate that has high virulence in animal models of infection and has been used extensively to study GBS pathogenesis. Two unusual featu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00504-20

    authors: Zhu L,Beres SB,Yerramilli P,Pruitt L,Cantu CC,Olsen RJ,Musser JM

    更新日期:2020-11-04 00:00:00

  • Characterization of hydrogenase II from the hyperthermophilic archaeon Pyrococcus furiosus and assessment of its role in sulfur reduction.

    abstract::The fermentative hyperthermophile Pyrococcus furiosus contains an NADPH-utilizing, heterotetrameric (alphabetagammadelta), cytoplasmic hydrogenase (hydrogenase I) that catalyzes both H(2) production and the reduction of elemental sulfur to H(2)S. Herein is described the purification of a second enzyme of this type, hy...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.7.1864-1871.2000

    authors: Ma K,Weiss R,Adams MW

    更新日期:2000-04-01 00:00:00

  • Genetic requirements for potassium ion-dependent colony spreading in Bacillus subtilis.

    abstract::Undomesticated strains of Bacillus subtilis exhibit extensive colony spreading on certain soft agarose media: first the formation of dendritic clusters of cells, followed by spreading (pellicle-like) growth to cover the entire surface. These phases of colonization are dependent on the level of potassium ion (K(+)) but...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.24.8462-8469.2005

    authors: Kinsinger RF,Kearns DB,Hale M,Fall R

    更新日期:2005-12-01 00:00:00

  • Reproduction and morphogenesis of Rickettsiella chironomi, an unusual intracellular procaryotic parasite of midge larvae.

    abstract::The reproduction and morphogenesis of Rickettsiella chironomi, an unusual procaryotic parasite of midge larvae, was studied with electron microscopy. The morphogenic cycle was similar to that found in chlamydia and consisted of four major cell types: (i) medium-sized spherical initial bodies (ca. 1 micrometers in diam...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.143.2.995-1002.1980

    authors: Federici BA

    更新日期:1980-08-01 00:00:00

  • Plasmid and transposon transfer to Thiobacillus ferrooxidans.

    abstract::The broad-host-range IncP plasmids RP4, R68.45, RP1::Tn501, and pUB307 were transferred to acidophilic, obligately chemolithotrophic Thiobacillus ferrooxidans from Escherichia coli by conjugation. A genetic marker of kanamycin resistance was expressed in T. ferrooxidans. Plasmid RP4 was transferred back to E. coli fro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.10.2892-2897.1994

    authors: Peng JB,Yan WM,Bao XZ

    更新日期:1994-05-01 00:00:00

  • Characterization of the Pseudomonas aeruginosa transcriptional response to phenylalanine and tyrosine.

    abstract::Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen often associated with chronic infections in the lungs of individuals with the heritable disease cystic fibrosis (CF). Previous work from our laboratory demonstrated that aromatic amino acids within CF lung secretions (sputum) not only serve as carbon and...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00112-10

    authors: Palmer GC,Palmer KL,Jorth PA,Whiteley M

    更新日期:2010-06-01 00:00:00

  • Isolation and characterization of a second exe operon required for extracellular protein secretion in Aeromonas hydrophila.

    abstract::Strain C5.84 is a Tn5-751 insertion mutant of Aeromonas hydrophila which is unable to secrete extracellular proteins, instead accumulating them in the periplasm (B. Jiang and S.P. Howard, J. Bacteriol. 173:1241-1249, 1991). A 3.5-kb BglII fragment which complements this mutation was isolated from the chromosome of the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.22.6819-6826.1994

    authors: Jahagirdar R,Howard SP

    更新日期:1994-11-01 00:00:00

  • FepA with globular domain deletions lacks activity.

    abstract::TonB-gated transporters have beta-barrels containing an amino-terminal globular domain that occludes the interior of the barrel. Mutations in the globular domain prevent transport of ligands across the outer membrane. Surprisingly, FepA with deletions of the globular domain (amino acids 3 to 150 and 17 to 150) was pre...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.19.5508-5512.2002

    authors: Vakharia HL,Postle K

    更新日期:2002-10-01 00:00:00

  • INTRACELLULAR BEHAVIOR OF HISTOPLASMA CAPSULATUM.

    abstract::Howard, Dexter H. (University of California, Los Angeles). Intracellular behavior of Histoplasma capsulatum. J. Bacteriol. 87:33-38. 1964.-The tinctorial characteristics and intracellular generation time of Histoplasma capsulatum within mouse histiocytes maintained in tissue culture were studied. The appearance of via...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.1.33-38.1964

    authors: HOWARD DH

    更新日期:1964-01-01 00:00:00

  • Occurrence of nickel in carbon monoxide dehydrogenase from Clostridium pasteurianum and Clostridium thermoaceticum.

    abstract::The carbon monoxide (CO) dehydrogenase activity band from Clostridium pasteurianum was shown to contain nickel by in situ activity staining of polyacrylamide gels. However, the majority of the nickel in cell extracts was found to electrophorese independently of CO dehydrogenase. Comparative analysis with Clostridium t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.149.2.561-566.1982

    authors: Drake HL

    更新日期:1982-02-01 00:00:00

  • Role of premature stop codons in bacterial evolution.

    abstract::When the stop codons TGA, TAA, and TAG are found in the second and third reading frames of a protein-encoding gene, they are considered premature stop codons (PSC). Deinococcus radiodurans disproportionately favored TGA more than the other two triplets as a PSC. The TGA triplet was also found more often in noncoding r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00682-08

    authors: Wong TY,Fernandes S,Sankhon N,Leong PP,Kuo J,Liu JK

    更新日期:2008-10-01 00:00:00

  • Enzymatic specificities and modes of action of the two catalytic domains of the XynC xylanase from Fibrobacter succinogenes S85.

    abstract::The xylanase XynC of Fibrobacter succinogenes S85 was recently shown to contain three distinct domains, A, B, and C (F. W. Paradis, H. Zhu, P. J. Krell, J. P. Phillips, and C. W. Forsberg, J. Bacteriol. 175:7666-7672, 1993). Domains A and B each bear an active site capable of hydrolyzing xylan, while domain C has no e...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.13.3885-3894.1994

    authors: Zhu H,Paradis FW,Krell PJ,Phillips JP,Forsberg CW

    更新日期:1994-07-01 00:00:00

  • A global investigation of the Bacillus subtilis iron-sparing response identifies major changes in metabolism.

    abstract::The Bacillus subtilis ferric uptake regulator (Fur) protein is the major sensor of cellular iron status. When iron is limiting for growth, derepression of the Fur regulon increases the cellular capacity for iron uptake and mobilizes an iron-sparing response mediated in large part by a small noncoding RNA named FsrA. F...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05990-11

    authors: Smaldone GT,Revelles O,Gaballa A,Sauer U,Antelmann H,Helmann JD

    更新日期:2012-05-01 00:00:00

  • Cyclic Dinucleotide-Controlled Regulatory Pathways in Streptomyces Species.

    abstract::The cyclic dinucleotides cyclic 3′,5′-diguanylate (c-di-GMP) and cyclic 3′,5′-diadenylate (c-di-AMP) have emerged as key components of bacterial signal transduction networks. These closely related second messengers follow the classical general principles of nucleotide signaling by integrating diverse signals into regu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章,评审

    doi:10.1128/JB.00423-15

    authors: Tschowri N

    更新日期:2016-01-01 00:00:00

  • Crystal structure and site-directed mutagenesis analyses of haloalkane dehalogenase LinB from Sphingobium sp. strain MI1205.

    abstract::The enzymes LinB(UT) and LinB(MI) (LinB from Sphingobium japonicum UT26 and Sphingobium sp. MI1205, respectively) catalyze the hydrolytic dechlorination of β-hexachlorocyclohexane (β-HCH) and yield different products, 2,3,4,5,6-pentachlorocyclohexanol (PCHL) and 2,3,5,6-tetrachlorocyclohexane-1,4-diol (TCDL), respecti...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02020-12

    authors: Okai M,Ohtsuka J,Imai LF,Mase T,Moriuchi R,Tsuda M,Nagata K,Nagata Y,Tanokura M

    更新日期:2013-06-01 00:00:00

  • Factors regulating competence in transformation of streptococci.

    abstract::Pakula, Roman (University of Toronto, Toronto, Ontario, Canada). Factors regulating competence in transformation of streptococci. J. Bacteriol. 90:1320-1324. 1965.-The highly transformable group H Streptococcus strain Challis produced an exocellular competence-provoking enzyme capable of converting to the state of com...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.90.5.1320-1324.1965

    authors: Pakula R

    更新日期:1965-11-01 00:00:00

  • Segmental message stabilization as a mechanism for differential expression from the Zymomonas mobilis gap operon.

    abstract::In Zymomonas mobilis, three- to fourfold more glyceraldehyde-3-phosphate dehydrogenase protein than phosphoglycerate kinase is needed for glycolysis because of differences in catalytic efficiency. Consistent with this requirement, higher levels of glyceraldehyde-3-phosphate dehydrogenase were observed with two-dimensi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.1.245-254.1991

    authors: Eddy CK,Keshav KF,An H,Utt EA,Mejia JP,Ingram LO

    更新日期:1991-01-01 00:00:00

  • Transcriptional regulation of the heat shock regulatory gene rpoH in Escherichia coli: involvement of a novel catabolite-sensitive promoter.

    abstract::A catabolite-sensitive promoter was found to be involved in transcription of the heat shock regulatory gene rpoH encoding the sigma 32 protein. Expression of lacZ from the operon fusion, rpoHp-lacZ, was partially inhibited by glucose added to the broth medium. Dissection of the rpoH promoter region allowed us to local...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.5.2710-2715.1990

    authors: Nagai H,Yano R,Erickson JW,Yura T

    更新日期:1990-05-01 00:00:00

  • Identification and Characterization of the Major Porin of Desulfovibrio vulgaris Hildenborough.

    abstract::Due in large part to their ability to facilitate the diffusion of a diverse range of solutes across the outer membrane (OM) of Gram-negative bacteria, the porins represent one of the most prominent and important bacterial membrane protein superfamilies. Notably, for the Gram-negative bacterium Desulfovibrio vulgaris H...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00286-17

    authors: Zeng L,Wooton E,Stahl DA,Walian PJ

    更新日期:2017-10-31 00:00:00

  • Electron microscopy of single-stranded structures in the DNA of competent Haemophilus influenzae cells.

    abstract::Chromosomal DNAs from exponential-phase and competent cells of Haemophilus influenzae were examined by electron microscopy to determine whether the chromosome undergoes structural changes during competence development. Single-stranded gaps and single-stranded tails formed in chromosomal DNA during competence developme...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.2.565-571.1987

    authors: McCarthy D,Kupfer DM

    更新日期:1987-02-01 00:00:00

  • Serology of the soluble antigens of Clostridium perfringens types A-F by agar-gel diffusion.

    abstract::Ellner, Paul D. (University of Vermont, Burlington) and Carolyn D. Bohan. Serology of the soluble antigens of Clostridium perfringens types A-F by agar-gel diffusion. J. Bacteriol. 83:284-296. 1962.-A serological study by agar-gel diffusion of the soluble antigens of 39 strains of the Clostridium perfringens group has...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.2.284-296.1962

    authors: ELLNER PD,BOHAN CD

    更新日期:1962-02-01 00:00:00

  • Defect in the formation of 70S ribosomes caused by lack of ribosomal protein L34 can be suppressed by magnesium.

    abstract::To elucidate the biological functions of the ribosomal protein L34, which is encoded by the rpmH gene, the rpmH deletion mutant of Bacillus subtilis and two suppressor mutants were characterized. Although the ΔrpmH mutant exhibited a severe slow-growth phenotype, additional mutations in the yhdP or mgtE gene restored ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01896-14

    authors: Akanuma G,Kobayashi A,Suzuki S,Kawamura F,Shiwa Y,Watanabe S,Yoshikawa H,Hanai R,Ishizuka M

    更新日期:2014-11-01 00:00:00

  • INFECTIOUS BOVINE RHINOTRACHEITIS VIRUS REPLICATION, CYTOPATHOLOGY, AND PLAQUE FORMATION IN THE PRESENCE AND ABSENCE OF NUCLEIC ACID ANALOGUES.

    abstract::Stevens, Jack G. (University of Washington, Seattle), and Neal B. Groman. Infectious bovine rhinotracheitis virus replication, cytopathology, and plaque formation in the presence and absence of nucleic acid analogues. J. Bacteriol. 87:446-453. 1964.-Cytopathology induced by infectious bovine rhinotracheitis (IBR) viru...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.2.446-453.1964

    authors: STEVENS JG,GROMAN NB

    更新日期:1964-02-01 00:00:00

  • New aerobactin-mediated iron uptake system in a septicemia-causing strain of Enterobacter cloacae.

    abstract::Unlike the great majority of the aerobactin-producing enteric bacteria documented in the literature, Enterobacter cloacae EK33, isolated from a case of human neonatal meningitis, did not show any homology at the DNA level with the prototype aerobactin system encoded by the ColV-K30 plasmid. However, both the nuclear m...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.12.5539-5544.1988

    authors: Crosa LM,Wolf MK,Actis LA,Sanders-Loehr J,Crosa JH

    更新日期:1988-12-01 00:00:00

  • N-Acyl-L-homoserine lactone autoinducers control production of an extracellular lipopeptide biosurfactant required for swarming motility of Serratia liquefaciens MG1.

    abstract::A nonswarming Serratia liquefaciens mutant deficient in serrawettin W2 production was constructed by transposon mutagenesis. Sequence homology indicated that insertion had occurred in gene swrA, which encodes a putative peptide synthetase. Expression of swrA is controlled by quorum sensing. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:

    authors: Lindum PW,Anthoni U,Christophersen C,Eberl L,Molin S,Givskov M

    更新日期:1998-12-01 00:00:00

  • Characterization of an A-factor-responsive repressor for amfR essential for onset of aerial mycelium formation in Streptomyces griseus.

    abstract::A-factor (2-isocapryloyl-3R-hydroxymethyl-gamma-butyrolactone) is essential for the initiation of aerial mycelium formation in Streptomyces griseus. amfR is one of the genes which, when cloned on a low-copy-number plasmid, suppresses the aerial mycelium-negative phenotype of an A-factor-deficient mutant of S. griseus....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.19.5085-5093.1998

    authors: Ueda K,Hsheh CW,Tosaki T,Shinkawa H,Beppu T,Horinouchi S

    更新日期:1998-10-01 00:00:00

  • Expression of hurP, a gene encoding a prospective site 2 protease, is essential for heme-dependent induction of bhuR in Bordetella bronchiseptica.

    abstract::Expression of the hurIR bhuRSTUV heme utilization locus in Bordetella bronchiseptica is coordinately controlled by the global iron-dependent regulator Fur and the extracytoplasmic function sigma factor HurI. Activation of HurI requires transduction of a heme-dependent signal via HurI, HurR, and BhuR, a three-component...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00629-07

    authors: King-Lyons ND,Smith KF,Connell TD

    更新日期:2007-09-01 00:00:00