Characterization of the Pseudomonas aeruginosa transcriptional response to phenylalanine and tyrosine.

Abstract:

:Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen often associated with chronic infections in the lungs of individuals with the heritable disease cystic fibrosis (CF). Previous work from our laboratory demonstrated that aromatic amino acids within CF lung secretions (sputum) not only serve as carbon and energy sources but also enhance synthesis of the cell signaling molecule Pseudomonas quinolone signal (PQS). The present study investigates the role of the aromatic amino acid-responsive regulator PhhR in mediating these phenotypes. Transcriptome analysis revealed that PhhR controls four putative transcriptional units (phhA, hpd, hmgA, and dhcA) involved in aromatic amino acid catabolism; however, genes involved in PQS biosynthesis were unaffected. The phhA, hpd, hmgA, and dhcA promoters were mapped by primer extension, and purified His(6)-PhhR was shown to bind the phhA, hpd, and dhcA promoters in vitro by use of electrophoretic mobility shift assays. Our work characterizes a transcriptional regulator of catabolic genes induced during P. aeruginosa growth in CF sputum.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Palmer GC,Palmer KL,Jorth PA,Whiteley M

doi

10.1128/JB.00112-10

subject

Has Abstract

pub_date

2010-06-01 00:00:00

pages

2722-8

issue

11

eissn

0021-9193

issn

1098-5530

pii

JB.00112-10

journal_volume

192

pub_type

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