Transfer and incorporation of genes controlling beta-D-galactosidase synthesis from Hfr and F' donors of Escherichia coli.

Abstract:

:Ganesan, Ann K. (Syntex Institute of Molecular Biology, Palo Alto, Calif.), and Boris Rotman. Transfer and incorporation of genes controlling beta-d-galactosidase synthesis from Hfr and F' donors of Escherichia coli. J. Bacteriol. 92:1378-1382. 1966.-Comparisons were made between Hfr(1) and F(13) donors with respect to the frequency of transfer and incorporation of genes controlling beta-d-galactosidase synthesis. The Hfr(1) donor transfers these genes as part of the chromosome, and the F(13) donor transfers them by F-duction. The criterion used for gene transfer was the acquisition by recipient cells of the ability to synthesize the enzyme, beta-d-galactosidase, measured by fluorogenic assays at the single-cell level. The criterion for incorporation was the formation of lac(+) recombinant colonies. It was found that the two types of donor showed the same frequency of gene transfer, but the probability of incorporation was 10-fold higher in F(13) matings than in Hfr(1) matings. In the former, between 46 and 97% of the merozygotes produced recombinant colonies; in the latter, 2 to 6% did so.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Ganesan AK,Rotman B

doi

10.1128/JB.92.5.1378-1382.1966

subject

Has Abstract

pub_date

1966-11-01 00:00:00

pages

1378-82

issue

5

eissn

0021-9193

issn

1098-5530

journal_volume

92

pub_type

杂志文章
  • EFFECT OF TEMPERATURE ON THE RATE OF GERMINATION IN BACILLUS CEREUS.

    abstract::Knaysi, Georges (Cornell University, Ithaca, N.Y.). Effect of temperature on the rate of germination in Bacillus cereus. J. Bacteriol. 87:619-622. 1964.-By use of the change of the spores from bright to dark, when observed in dark contrast with a phase microscope, as a criterion for the incipience (first stage) of ger...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.3.619-622.1964

    authors: KNAYSI G

    更新日期:1964-03-01 00:00:00

  • Structural study of binding of flagellin by Toll-like receptor 5.

    abstract::In order to predict the binding regions within the complex formed by Toll-like receptor 5 (TLR-5) and flagellin, a complementary hydropathy between the two proteins was sought. A region common to the flagellins of Salmonella enterica serovar Typhimurium, Pseudomonas aeruginosa, and Listeria monocytogenes was shown to ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.14.4243-4247.2003

    authors: Jacchieri SG,Torquato R,Brentani RR

    更新日期:2003-07-01 00:00:00

  • Perturbation of nifT expression in Klebsiella pneumoniae has limited effect on nitrogen fixation.

    abstract::In the nitrogenase system of Klebsiella pneumoniae, nifT is located between nifDK, the structural genes for dinitrogenase, and nifY, whose product is involved in nitrogenase maturation. It is, therefore, a reasonable hypothesis that the NifT protein might also have a role in the maturation of nitrogenase. However, the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.10.2975-2977.1996

    authors: Simon HM,Homer MJ,Roberts GP

    更新日期:1996-05-01 00:00:00

  • Effects of pancreatin on the growth of reovirus.

    abstract::Wallis, Craig (Baylor University College of Medicine, Houston, Tex.), Joseph L. Melnick, and Fred Rapp. Effects of pancreatin on the growth of reovirus. J. Bacteriol. 92:155-160. 1966.-The influence of pancreatin and other proteolytic enzymes on the growth, plaque formation, and antigenicity of reovirus was studied. S...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.92.1.155-160.1966

    authors: Wallis C,Melnick JL,Rapp F

    更新日期:1966-07-01 00:00:00

  • Rhs elements of Escherichia coli K-12: complex composites of shared and unique components that have different evolutionary histories.

    abstract::The complete sequences of the RhsB and RhsC elements of Escherichia coli K-12 have been determined. These sequence data reveal a new repeated sequence, called H-rpt (Hinc repeat), which is distinct from the Rhs core repetition that is found in all five Rhs elements. H-rpt is found in RhsB, RhsC, and RhsE. Characteriza...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.10.2799-2808.1993

    authors: Zhao S,Sandt CH,Feulner G,Vlazny DA,Gray JA,Hill CW

    更新日期:1993-05-01 00:00:00

  • Oligomerization of the bacteriophage lambda S protein in the inner membrane of Escherichia coli.

    abstract::Western blot (immunoblot) analysis of cell extracts from induced bacteriophage lambda lysogens probed with S-protein-specific antibody (raised against an S--beta-galactosidase fusion protein) demonstrated that the bacteriophage lambda S protein begins to appear 10 min after phage induction and is localized to the inne...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.2.912-921.1990

    authors: Zagotta MT,Wilson DB

    更新日期:1990-02-01 00:00:00

  • Anaerobic degradation of the benzene nucleus by a facultatively anaerobic microorganism.

    abstract::A bacterium was isolated by elective culture with p-hydroxybenzoate as substrate and nitrate as electron acceptor. It grew either aerobically or anaerobically, by nitrate respiration, on a range of aromatic compounds. The organism was identified as a pseudomonad and was given the trivial name Pseudomonas PN-1. Benzoat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.102.2.430-437.1970

    authors: Taylor BF,Campbell WL,Chinoy I

    更新日期:1970-05-01 00:00:00

  • A mer-lux transcriptional fusion for real-time examination of in vivo gene expression kinetics and promoter response to altered superhelicity.

    abstract::We constructed mercury resistance operon-luciferase (mer-lux) transcriptional fusion plasmids to evaluate in vivo gene expression rates of the mer structural gene promoter (PTPCAD) of transposon Tn21. In vivo gene expression kinetics corresponded well with those previously determined in vitro, yielding an apparent K0....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.24.8094-8101.1992

    authors: Condee CW,Summers AO

    更新日期:1992-12-01 00:00:00

  • Comprehensive transcriptome analysis of the periodontopathogenic bacterium Porphyromonas gingivalis W83.

    abstract::High-density tiling microarray and RNA sequencing technologies were used to analyze the transcriptome of the periodontopathogenic bacterium Porphyromonas gingivalis. The compiled P. gingivalis transcriptome profiles were based on total RNA samples isolated from three different laboratory culturing conditions, and the ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06385-11

    authors: Høvik H,Yu WH,Olsen I,Chen T

    更新日期:2012-01-01 00:00:00

  • Generic identification of L-forms by polyacrylamide gel electrophoretic comparison of extracts from parent strains and their derived L-forms.

    abstract::A polyacrylamide gel electrophoretic method was used for identification of L-forms in the genera Streptococcus, Staphylococcus, and Proteus, by comparing phenol-acetic acid-water extracts of homologous parent L-form pairs to one another and to other pairs. The method requires only milligram quantities of material for ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.1.71-77.1969

    authors: King JR,Theodore TS,Cole RM

    更新日期:1969-10-01 00:00:00

  • Cloning, sequencing, and enhanced expression of the dihydropteroate synthase gene of Escherichia coli MC4100.

    abstract::The Escherichia coli gene coding for dihydropteroate synthase (DHPS) has been cloned and sequenced. The protein has 282 amino acids and a compositional molecular mass of 30,314 daltons. Increased expression of the enzyme was realized by using a T7 expression system. The enzyme was purified and crystallized. A temperat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.18.5961-5970.1992

    authors: Dallas WS,Gowen JE,Ray PH,Cox MJ,Dev IK

    更新日期:1992-09-01 00:00:00

  • Cell wall modification resulting from in vitro induction of L-phase variants of Nocardia asteroides.

    abstract::The chemical composition of the cell walls of several L-form revertants derived from Nocardia asteroides 10905 was determined at different stages of growth. It was observed that each L-form revertant had a cell well that differed from that of the parental strain when grown under identical conditions. In some strains t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.148.2.600-609.1981

    authors: Beaman BL,Bourgeois AL,Moring SE

    更新日期:1981-11-01 00:00:00

  • The C-Terminal Zwitterionic Sequence of CotB1 Is Essential for Biosilicification of the Bacillus cereus Spore Coat.

    abstract:UNLABELLED:Silica is deposited in and around the spore coat layer of Bacillus cereus, and enhances the spore's acid resistance. Several peptides and proteins, including diatom silaffin and silacidin peptides, are involved in eukaryotic silica biomineralization (biosilicification). Homologous sequence search revealed a ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00447-15

    authors: Motomura K,Ikeda T,Matsuyama S,Abdelhamid MA,Tanaka T,Ishida T,Hirota R,Kuroda A

    更新日期:2015-10-26 00:00:00

  • Oxacillin-induced inhibition of protein and RNA synthesis in a tolerant Staphylococcus aureus isolate.

    abstract::A clinical isolate of Staphylococcus aureus was found to be tolerant (MBC much greater than MIC) to a number of beta-lactam antibiotics, including oxacillin. Biophotometric analysis showed that a number of concentrations of oxacillin were capable of stimulating rapid cellular lysis in this organism, but the extent of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.4.1831-1836.1988

    authors: Jablonski PE,Mychajlonka M

    更新日期:1988-04-01 00:00:00

  • Maleylacetate reductase of Pseudomonas sp. strain B13: specificity of substrate conversion and halide elimination.

    abstract::Maleylacetate reductase (EC 1.3.1.32) plays a major role in the degradation of chloroaromatic compounds by channelling maleylacetate and some chlorinated derivatives into the 3-oxoadipate pathway. Several substituted maleylacetates were prepared in situ by alkaline or enzymatic hydrolysis of dienelactones as the precu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.2.320-325.1995

    authors: Kaschabek SR,Reineke W

    更新日期:1995-01-01 00:00:00

  • Deficiency of the Rgg regulator promotes H2O2 resistance, AhpCF-mediated H2O2 decomposition, and virulence in Streptococcus pyogenes.

    abstract::Streptococcus pyogenes (group A streptococcus [GAS]), a catalase-negative gram-positive bacterium, is aerotolerant and survives H2O2 exposures that kill many catalase-positive bacteria. The molecular basis of the H2O2 resistance is poorly known. Here, we demonstrate that serotype M49 GAS lacking the Rgg regulator is m...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01843-07

    authors: Pulliainen AT,Hytönen J,Haataja S,Finne J

    更新日期:2008-05-01 00:00:00

  • A type II protein secretory pathway required for levansucrase secretion by Gluconacetobacter diazotrophicus.

    abstract::The endophytic diazotroph Gluconacetobacter diazotrophicus secretes a constitutively expressed levansucrase (LsdA, EC 2.4.1.10) to utilize plant sucrose. LsdA, unlike other extracellular levansucrases from gram-negative bacteria, is transported to the periplasm by a signal-peptide-dependent pathway. We identified an u...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.15.5031-5039.2004

    authors: Arrieta JG,Sotolongo M,Menéndez C,Alfonso D,Trujillo LE,Soto M,Ramírez R,Hernández L

    更新日期:2004-08-01 00:00:00

  • Patterns of protein production in Myxococcus xanthus during spore formation induced by glycerol, dimethyl sulfoxide, and phenethyl alcohol.

    abstract::Spore formation of Myxococcus xanthus can occur not only on agar plates during fruiting body formation, but also in a liquid culture by simply adding glycerol, dimethyl sulfoxide, or phenethyl alcohol to the culture. This chemically-induced spore formation occurs synchronously and much faster than that occurring durin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.144.3.1076-1082.1980

    authors: Komano T,Inouye S,Inouye M

    更新日期:1980-12-01 00:00:00

  • Nucleotide sequence of aceK, the gene encoding isocitrate dehydrogenase kinase/phosphatase.

    abstract::In Escherichia coli, the phosphorylation and dephosphorylation of isocitrate dehydrogenase (IDH) are catalyzed by a bifunctional protein kinase/phosphatase. We have determined the nucleotide sequence of aceK, the gene encoding IDH kinase/phosphatase. This gene consists of a single open reading frame of 1,734 base pair...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.6.2763-2769.1988

    authors: Klumpp DJ,Plank DW,Bowdin LJ,Stueland CS,Chung T,LaPorte DC

    更新日期:1988-06-01 00:00:00

  • Control of transcription of gal repressor and isorepressor genes in Escherichia coli.

    abstract::Two regulatory proteins, Gal repressor and isorepressor, control the expression of the gal and mgl operons in Escherichia coli. The transcription start sites for galR and galS, the genes for the repressor and isorepressor, were determined by primer extension of in vivo transcripts. Study of the promoter-lacZ gene fusi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.1.251-258.1993

    authors: Weickert MJ,Adhya S

    更新日期:1993-01-01 00:00:00

  • Purification and properties of mutant and wild-type diaphorases from Diplococcus pneumoniae.

    abstract::Optochin-resistant mutant and wild-type diaphorases were purified approximately 300-fold by a combination of batch adsorption and column chromatography with diethylaminoethyl cellulose, and were characterized with regard to their pH optima, sensitivity to optochin inhibition and heat inactivation, Michaelis constants ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.96.4.1029-1036.1968

    authors: Kreger AS,Olsen RH

    更新日期:1968-10-01 00:00:00

  • Transcriptional organization of the phycocyanin subunit gene clusters of the cyanobacterium Anacystis nidulans UTEX 625.

    abstract::The phycocyanin subunit gene cluster is duplicated on the chromosome of the cyanobacterium Anacystis nidulans UTEX 625. The two gene clusters cpcB1A1 (left) and cpcB2A2 (right) are separated by about 2,500 base pairs, and in each cluster the beta-subunit gene is located upstream from the alpha-subunit gene. Filter hyb...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.7.2961-2970.1988

    authors: Kalla SR,Lind LK,Lidholm J,Gustafsson P

    更新日期:1988-07-01 00:00:00

  • Chemotaxis arrays in Vibrio species and their intracellular positioning by the ParC/ParP system.

    abstract::Most motile bacteria are able to bias their movement towards more favorable environments or to escape from obnoxious substances by a process called chemotaxis. Chemotaxis depends on a chemosensory system that is able to sense specific environmental signals and generate a behavioral response. Typically, the signal is t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00793-17

    authors: Ringgaard S,Yang W,Alvarado A,Schirner K,Briegel A

    更新日期:2018-03-12 00:00:00

  • Nucleotide sequence of the tcmII-tcmIV region of the tetracenomycin C biosynthetic gene cluster of Streptomyces glaucescens and evidence that the tcmN gene encodes a multifunctional cyclase-dehydratase-O-methyl transferase.

    abstract::Mutations in the tcmII-tcmIV region of the Streptomyces glaucescens chromosome block the C-3 and C-8 O-methylations of the polyketide antibiotic tetracenomycin C (Tcm C). The nucleotide sequence of this region reveals the presence of two genes, tcmN and tcmO, whose deduced protein products display similarity to the hy...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.6.1810-1820.1992

    authors: Summers RG,Wendt-Pienkowski E,Motamedi H,Hutchinson CR

    更新日期:1992-03-01 00:00:00

  • Repair of x-ray-induced deoxyribonucleic acid single-strand breaks in xth mutants of Escherichia coli.

    abstract::An exonuclease III-deficient strain of Escherichia coli K-12, BW2001 (xthA11), was unable to perform rapid repair of X-ray-induced deoxyribonucleic acid single-strand breaks and appeared to have a defect in the priming of the 3'-termini necessary for initiation of repair synthesis at the breaks. This defect cannot be ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.141.3.1424-1427.1980

    authors: Seeberg E,Steinum AL

    更新日期:1980-03-01 00:00:00

  • Chromosomal mapping of Bacillus thuringiensis by transduction.

    abstract::Three groups of linked markers were mapped in Bacillus thuringiensis 4042B by using two-, three-, and four-factor crosses mediated by the temperate bacteriophages TP-13 and TP-18. The order of markers was (trp-11, trp-2)-(leu-1, leu-2)-his-1-(lys-1, lys-2)-cys-1 in the first group; met-1-(argCl, argOl)-met-2-(pyr-1, p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.157.3.746-750.1984

    authors: Barsomian GD,Robillard NJ,Thorne CB

    更新日期:1984-03-01 00:00:00

  • Molecular characterization of the Lactococcus lactis LlaKR2I restriction-modification system and effect of an IS982 element positioned between the restriction and modification genes.

    abstract::The nucleotide sequence of the plasmid-encoded LlaKR2I restriction-modification (R-M) system of Lactococcus lactis subsp. lactis biovar diacetylactis KR2 was determined. This R-M system comprises divergently transcribed endonuclease (llaKR2IR) and methyltransferase (llaKR2IM) genes; located in the intergenic region is...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.22.5844-5854.1998

    authors: Twomey DP,McKay LL,O'Sullivan DJ

    更新日期:1998-11-01 00:00:00

  • Mutants suppressing novobiocin hypersensitivity of a mukB null mutation.

    abstract::The mukB gene is essential for the partitioning of sister chromosomes in Escherichia coli. A mukB null mutant is hypersensitive to the DNA gyrase inhibitor novobiocin. In this work, we isolated mutants suppressing the novobiocin hypersensitivity of the mukB null mutation. All suppressor mutations are localized in or n...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.13.3690-3695.2003

    authors: Adachi S,Hiraga S

    更新日期:2003-07-01 00:00:00

  • Complete genome sequence of Lactobacillus plantarum JDM1.

    abstract::Lactobacillus plantarum is a lactic acid bacterium (LAB) species commonly used as a probiotic. We have sequenced the genome of Lactobacillus plantarum JDM1, which is a Chinese commercial LAB with several probiotic functions, using a GS 20 system. We recommend that each commercial probiotic strain should undergo comple...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00587-09

    authors: Zhang ZY,Liu C,Zhu YZ,Zhong Y,Zhu YQ,Zheng HJ,Zhao GP,Wang SY,Guo XK

    更新日期:2009-08-01 00:00:00

  • Cloning and sequencing of the sacA gene: characterization of a sucrase from Zymomonas mobilis.

    abstract::The Zymomonas mobilis gene (sacA) encoding a protein with sucrase activity has been cloned in Escherichia coli and its nucleotide sequence has been determined. Potential ribosome-binding site and promoter sequences were identified in the region upstream of the gene which were homologous to E. coli and Z. mobilis conse...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.12.6727-6735.1990

    authors: Gunasekaran P,Karunakaran T,Cami B,Mukundan AG,Preziosi L,Baratti J

    更新日期:1990-12-01 00:00:00