Nucleotide sequence of aceK, the gene encoding isocitrate dehydrogenase kinase/phosphatase.

Abstract:

:In Escherichia coli, the phosphorylation and dephosphorylation of isocitrate dehydrogenase (IDH) are catalyzed by a bifunctional protein kinase/phosphatase. We have determined the nucleotide sequence of aceK, the gene encoding IDH kinase/phosphatase. This gene consists of a single open reading frame of 1,734 base pairs preceded by a Shine-Dalgarno ribosome-binding site. Examination of the deduced amino acid sequence of IDH kinase/phosphatase revealed sequences which are similar to the consensus sequence for ATP-binding sites. This protein did not, however, exhibit the extensive sequence homologies which are typical of other protein kinases. Multiple copies of the REP family of repetitive extragenic elements were found within the intergenic region between aceA (encoding isocitrate lyase) and aceK. These elements have the potential for combining to form an exceptionally stable stem-loop structure (delta G = -54 kcal/mol [ca. -226 kJ/mol]) in the mRNA. This structure, which masks the ribosome-binding site and start codon for aceK, may contribute to the downshift in expression observed between aceA and aceK. Another potential stem-loop structure (delta G = -29 kcal/mol [ca. 121 kJ/mol]), unrelated to the REP sequences, was found within aceK.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Klumpp DJ,Plank DW,Bowdin LJ,Stueland CS,Chung T,LaPorte DC

doi

10.1128/jb.170.6.2763-2769.1988

subject

Has Abstract

pub_date

1988-06-01 00:00:00

pages

2763-9

issue

6

eissn

0021-9193

issn

1098-5530

journal_volume

170

pub_type

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