Abstract:
:The mukB gene is essential for the partitioning of sister chromosomes in Escherichia coli. A mukB null mutant is hypersensitive to the DNA gyrase inhibitor novobiocin. In this work, we isolated mutants suppressing the novobiocin hypersensitivity of the mukB null mutation. All suppressor mutations are localized in or near the gyrB gene, and the four tested clones have an amino acid substitution in the DNA gyrase beta subunit. We found that in the mukB mutant, the process of sister chromosome segregation is strikingly hypersensitive to novobiocin; however, the effect of novobiocin on growth, which was measured by culture turbidity, is the same as that of the wild-type strain.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Adachi S,Hiraga Sdoi
10.1128/jb.185.13.3690-3695.2003keywords:
subject
Has Abstractpub_date
2003-07-01 00:00:00pages
3690-5issue
13eissn
0021-9193issn
1098-5530journal_volume
185pub_type
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更新日期:2012-02-01 00:00:00
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更新日期:1972-03-01 00:00:00
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更新日期:2004-04-01 00:00:00
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更新日期:1990-10-01 00:00:00
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更新日期:1981-02-01 00:00:00
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更新日期:2007-08-01 00:00:00
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更新日期:1993-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:2008-10-01 00:00:00
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更新日期:1991-04-01 00:00:00
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更新日期:1997-01-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1994-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.9.2517-2524.1994
更新日期:1994-05-01 00:00:00