Expression of the Bacillus subtilis acsA gene: position and sequence context affect cre-mediated carbon catabolite repression.

Abstract:

:In Bacillus subtilis, carbon catabolite repression (CCR) of many genes is mediated at cis-acting carbon repression elements (cre) by the catabolite repressor protein CcpA. Mutations in transcription-repair coupling factor (mfd) partially relieve CCR at cre sites located downstream of transcriptional start sites by abolishing the Mfd-mediated displacement of RNA polymerase stalled at cre sites which act as transcriptional roadblocks. Although the acsA cre is centered 44.5 bp downstream of the acsA transcriptional start site, CCR of acsA expression is not affected by an mfd mutation. When the acsA cre is centered 161.5 bp downstream of the transcriptional start site for the unregulated tms promoter, CCR is partially relieved by the mfd mutation. Since CCR mediated at an acsA cre centered 44.5 bp downstream of the tms start site is not affected by the mfd mutation, the inability of Mfd to modulate CCR of acsA expression most likely results from the location of the acsA cre. Higher levels of CCR were found to occur at cre sites flanked by A+T-rich sequences than at cre sites bordered by G and C nucleotides. This suggests that nucleotides adjacent to the proposed 14-bp cre consensus sequence participate in the formation of the CcpA catabolite repression complex at cre sites. Examination of CCR of acsA expression revealed that this regulation required the Crh and seryl-phosphorylated form of the HPr proteins but not glucose kinase.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Zalieckas JM,Wray LV Jr,Fisher SH

doi

10.1128/JB.180.24.6649-6654.1998

subject

Has Abstract

pub_date

1998-12-01 00:00:00

pages

6649-54

issue

24

eissn

0021-9193

issn

1098-5530

journal_volume

180

pub_type

杂志文章
  • Suppression of engulfment defects in bacillus subtilis by elevated expression of the motility regulon.

    abstract::During Bacillus subtilis sporulation, the transient engulfment defect of spoIIB strains is enhanced by spoVG null mutations and suppressed by spoVS null mutations. These mutations have opposite effects on expression of the motility regulon, as the spoVG mutation reduces and the spoVS mutation increases sigmaD-directed...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.3.1159-1164.2006

    authors: Perez AR,Abanes-De Mello A,Pogliano K

    更新日期:2006-02-01 00:00:00

  • Adaptation of Salmonella typhimurium mutants containing uncoupled enzyme IIGlc to glucose-limited conditions.

    abstract::Uncoupled enzyme IIGlc of the phosphoenolpyruvate (PEP):glucose phosphotransferase system (PTS) in Salmonella typhimurium is able to catalyze glucose transport in the absence of PEP-dependent phosphorylation. As a result of the ptsG mutation, the apparent Km of the system for glucose transport is increased about 1,000...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.9.4783-4789.1990

    authors: Ruijter GJ,Postma PW,van Dam K

    更新日期:1990-09-01 00:00:00

  • Genome sequence of the bacterium Streptomyces davawensis JCM 4913 and heterologous production of the unique antibiotic roseoflavin.

    abstract::Streptomyces davawensis JCM 4913 synthesizes the antibiotic roseoflavin, a structural riboflavin (vitamin B(2)) analog. Here, we report the 9,466,619-bp linear chromosome of S. davawensis JCM 4913 and a 89,331-bp linear plasmid. The sequence has an average G+C content of 70.58% and contains six rRNA operons (16S-23S-5...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01592-12

    authors: Jankowitsch F,Schwarz J,Rückert C,Gust B,Szczepanowski R,Blom J,Pelzer S,Kalinowski J,Mack M

    更新日期:2012-12-01 00:00:00

  • PHAGE ISOLATED FROM LYSOGENIC BACILLUS ANTHRACIS.

    abstract::Buck, C. A. (Kansas State University, Manhattan), R. L. Anacker, F. S. Newman, and A. Eisenstark. Phage isolated from lysogenic Bacillus anthracis. J. Bacteriol. 85:1423-1430. 1963.-Bacillus anthracis cultures (a total of 311) were obtained from several laboratories throughout the world; 139 of these were screened for...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.85.6.1423-1430.1963

    authors: BUCK CA,ANACKER RL,NEWMAN FS,EISENSTARK A

    更新日期:1963-06-01 00:00:00

  • Bradyrhizobium japonicum glnB, a putative nitrogen-regulatory gene, is regulated by NtrC at tandem promoters.

    abstract::The glnB gene from Bradyrhizobium japonicum, the endosymbiont of soybeans (Glycine max), was isolated and sequenced, and its expression was examined under various culture conditions and in soybean nodules. The B. japonicum glnB gene encodes a 12,237-dalton polypeptide that is highly homologous to the glnB gene product...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.10.5638-5645.1989

    authors: Martin GB,Thomashow MF,Chelm BK

    更新日期:1989-10-01 00:00:00

  • Substrate specificity of naphthalene dioxygenase: effect of specific amino acids at the active site of the enzyme.

    abstract::The three-component naphthalene dioxygenase (NDO) enzyme system carries out the first step in the aerobic degradation of naphthalene by Pseudomonas sp. strain NCIB 9816-4. The three-dimensional structure of NDO revealed that several of the amino acids at the active site of the oxygenase are hydrophobic, which is consi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.6.1641-1649.2000

    authors: Parales RE,Lee K,Resnick SM,Jiang H,Lessner DJ,Gibson DT

    更新日期:2000-03-01 00:00:00

  • A second global regulator gene (arcB) mediating repression of enzymes in aerobic pathways of Escherichia coli.

    abstract::In Escherichia coli anaerobic growth lowers the basal or induced levels of numerous enzymes associated with aerobic metabolism. Mutations in arcA (dye) at min 0 relieve this pleiotropic anaerobic repression and render the cell sensitive to the redox dye toluidine blue. In this study we identified a second pleiotropic ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.2.868-873.1989

    authors: Iuchi S,Cameron DC,Lin EC

    更新日期:1989-02-01 00:00:00

  • Influence of SV40 Genome on the Replication of an Adenovirus-SV40 "Hybrid" Population.

    abstract::Feldman, L. A. (Baylor University College of Medicine, Houston, Tex.), J. L. Melnick, and F. Rapp. Influence of SV40 genome on the replication of an adenovirus-SV40 "hybrid" population. J. Bacteriol. 90:778-782. 1965.-Replication of a type 7 adenovirus-SV40 hybrid population in primary African green monkey kidney cell...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.90.3.778-782.1965

    authors: Feldman LA,Melnick JL,Rapp F

    更新日期:1965-09-01 00:00:00

  • Ultrastructure of the cell wall and cytoplasmic membrane of gram-negative bacteria with different fixation techniques.

    abstract::The ultrastructure of the cytoplasmic membrane and cell wall of two strains of Escherichia coli, Proteus morganii, P. vulgaris, Acinetobacter anitratum, Moraxella lacunata, Erwinia amylovora, Acinetobacter sp., and of a plant pathogen, unclassified gram-negative, fixed by the Ryter-Kellenberger procedure, was found to...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.113.2.953-962.1973

    authors: Silva MT,Sousa JC

    更新日期:1973-02-01 00:00:00

  • Role of lithium ions in proline transport in Escherichia coli.

    abstract::Mechanisms of Li+ stimulation of proline transport were studied in cells of Escherichia coli 7 and NR70, a mutant of strain 7 lacking adenosine triphosphatase (EC 3.6.1.3). An electrochemical potential difference of Li+ induced in an inward direction of energy-depleted cells caused a transient uptake of proline depend...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.139.2.560-564.1979

    authors: Kayama-Gonda Y,Kawasaki T

    更新日期:1979-08-01 00:00:00

  • Whole-genome shotgun sequencing of Lactobacillus rhamnosus MTCC 5462, a strain with probiotic potential.

    abstract::Lactobacillus rhamnosus MTCC 5462 was isolated from infant gastrointestinal flora. The strain exhibited an ability to reduce cholesterol and stimulate immunity. The strain has exhibited positive results in alleviating gastrointestinal discomfort and good potential as a probiotic. We sequenced the whole genome of the s...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06644-11

    authors: Prajapati JB,Khedkar CD,Chitra J,Suja S,Mishra V,Sreeja V,Patel RK,Ahir VB,Bhatt VD,Sajnani MR,Jakhesara SJ,Koringa PG,Joshi CG

    更新日期:2012-03-01 00:00:00

  • Molecular cloning of structural and immunity genes for megacins A-216 and A-19213 in Bacillus megaterium.

    abstract::A host-vector system was developed for molecular cloning in Bacillus megaterium and used to clone the structural and immunity genes for megacins A-216 and A-19213. Recombinant clones that expressed immunity only or both immunity to and production of each megacin were obtained. Restriction mapping of native megacinogen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.160.3.854-859.1984

    authors: Von Tersch MA,Carlton BC

    更新日期:1984-12-01 00:00:00

  • Acceleration of starvation- and glycerol-induced myxospore formation by prior heat shock in Myxococcus xanthus.

    abstract::The effect of heat shock on Myxococcus xanthus was investigated during both glycerol- and starvation-induced development. Cells heat shocked at 40 degrees C for 1 h prior to a development-inducing signal displayed an accelerated rate of myxospore formation at 30 degrees C. Additionally, M. xanthus cells heat shocked p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.11.5200-5207.1988

    authors: Killeen KP,Nelson DR

    更新日期:1988-11-01 00:00:00

  • Complete genome sequence of Carnobacterium sp. 17-4.

    abstract::Members of the carnobacteria have been extensively studied as probiotic cultures in aquacultures and protective cultures in seafood, diary, and meat. We report on the finished genome sequence of Carnobacterium sp. 17-4, which has been isolated from permanently cold seawater. The genetic information reveals a new circu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05113-11

    authors: Voget S,Klippel B,Daniel R,Antranikian G

    更新日期:2011-07-01 00:00:00

  • (S)-3-hydroxy-3-methylglutaryl coenzyme A reductase, a product of the mva operon of Pseudomonas mevalonii, is regulated at the transcriptional level.

    abstract::We have cloned and sequenced a 505-base-pair (bp) segment of DNA situated upstream of mvaA, the structural gene for (S)-3-hydroxy-3-methylglutaryl coenzyme A reductase (EC 1.1.1.88) of Pseudomonas mevalonii. The DNA segment that we characterized includes the promoter region for the mva operon. Nuclease S1 mapping and ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.10.5567-5571.1989

    authors: Wang YL,Beach MJ,Rodwell VW

    更新日期:1989-10-01 00:00:00

  • Homology between Escherichia coli plasmids ColE1 and p15A.

    abstract::The location and extent of the homology between plasmids ColE1 and p15A were determined by analysis of heteroduplexes formed between them as well as with a related plasmid, pBR322, and by hybridization of radioactive deoxyribonucleic acids to restriction fragments of p15A and ColE1. The homology between the plasmids c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.145.3.1305-1309.1981

    authors: Bird RE

    更新日期:1981-03-01 00:00:00

  • Cellular characterization of the primosome and rep helicase in processing and restoration of replication following arrest by UV-induced DNA damage in Escherichia coli.

    abstract::Following arrest by UV-induced DNA damage, replication is restored through a sequence of steps that involve partial resection of the nascent DNA by RecJ and RecQ, branch migration and processing of the fork DNA surrounding the lesion by RecA and RecF-O-R, and resumption of DNA synthesis once the blocking lesion has be...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00290-12

    authors: Courcelle CT,Landstrom AJ,Anderson B,Courcelle J

    更新日期:2012-08-01 00:00:00

  • Identical, independent, and opposing roles of ppGpp and DksA in Escherichia coli.

    abstract::The recent discovery that the protein DksA acts as a coregulator of genes controlled by ppGpp led us to investigate the similarities and differences between the relaxed phenotype of a ppGpp-deficient mutant and the phenotype of a strain lacking DksA. We demonstrate that the absence of DksA and ppGpp has similar effect...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00330-07

    authors: Magnusson LU,Gummesson B,Joksimović P,Farewell A,Nyström T

    更新日期:2007-07-01 00:00:00

  • Localization of low-molecular-weight basic proteins in Bacillus megaterium spores by cross-linking with ultraviolet light.

    abstract::Two low-molecular-weight basic proteins, termed A and B proteins, comprise about 15% of the protein of dormant spores of Bacillus megaterium. Irradiation of intact dormant spores with ultraviolet light results in covalent cross-linking of the A and B proteins to other spore macromolecules. The cross-linked A and B pro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.139.2.486-494.1979

    authors: Setlow B,Setlow P

    更新日期:1979-08-01 00:00:00

  • Evidence for specific inhibition of translocation of aminoacyl-transfer ribonucleic acid by the pretreatment of ribosomes of Bacillus subtilis with p-chloromercuribenzoate.

    abstract::The biological activities of Bacillus subtilis ribosomes pretreated with 0.5, 1, or 2 mmp-chloromercuribenzoate (PCMB) were examined. The ribosomes treated with 1 or 2 mm PCMB lost their capacity to synthesize polyphenylalanine as well as the capacity to bind phenylalanyl-transfer ribonucleic acid. On the other hand, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.112.1.188-194.1972

    authors: Ranu RS,Kaji A

    更新日期:1972-10-01 00:00:00

  • Deficiency of the Rgg regulator promotes H2O2 resistance, AhpCF-mediated H2O2 decomposition, and virulence in Streptococcus pyogenes.

    abstract::Streptococcus pyogenes (group A streptococcus [GAS]), a catalase-negative gram-positive bacterium, is aerotolerant and survives H2O2 exposures that kill many catalase-positive bacteria. The molecular basis of the H2O2 resistance is poorly known. Here, we demonstrate that serotype M49 GAS lacking the Rgg regulator is m...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01843-07

    authors: Pulliainen AT,Hytönen J,Haataja S,Finne J

    更新日期:2008-05-01 00:00:00

  • Identification and functional characterization of arylamine N-acetyltransferases in eubacteria: evidence for highly selective acetylation of 5-aminosalicylic acid.

    abstract::Arylamine N-acetyltransferase activity has been described in various bacterial species. Bacterial N-acetyltransferases, including those from bacteria of the gut flora, may be involved in the metabolism of xenobiotics, thereby exerting physiopathological effects. We characterized these enzymes further by steady-state k...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.11.3417-3427.2001

    authors: Deloménie C,Fouix S,Longuemaux S,Brahimi N,Bizet C,Picard B,Denamur E,Dupret JM

    更新日期:2001-06-01 00:00:00

  • In vivo observation of cell division of anaerobic hyperthermophiles by using a high-intensity dark-field microscope.

    abstract::To study growth and cell division of anaerobic hyperthermophilic archaea in vivo, a cultivation technique using glass capillaries was developed. At temperatures of 90 to 98 degrees C, at least 10 successive cell divisions of Pyrodictium abyssi TAG 11 were documented. Cells divide by binary fission. Visualized under a ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.16.5114-5118.1999

    authors: Horn C,Paulmann B,Kerlen G,Junker N,Huber H

    更新日期:1999-08-01 00:00:00

  • Complementation and characterization of chemotaxis mutants of Bacillus subtilis.

    abstract::A set of chemotaxis mutants of Bacillus subtilis was complemented by using SP beta c2 transducing bacteriophage either containing cloned segments of DNA or derived from abnormal excision of SP beta c2 dl2::Tn917 inserted into the chemotaxis region. Representative mutants were characterized in capillary assays for chem...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.164.2.802-810.1985

    authors: Ordal GW,Parker HM,Kirby JR

    更新日期:1985-11-01 00:00:00

  • Aspartate aminotransferase activity is required for aspartate catabolism and symbiotic nitrogen fixation in Rhizobium meliloti.

    abstract::A mutant of Rhizobium meliloti, 4R3, which is unable to grow on aspartate has been isolated. The defect is specific to aspartate utilization, since 4R3 is not an auxotroph and grows as well as its parent strain on other carbon and nitrogen sources. The defect was correlated with an inability to fix nitrogen within nod...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.9.2879-2887.1991

    authors: Rastogi VK,Watson RJ

    更新日期:1991-05-01 00:00:00

  • Threonine degradation by Serratia marcescens.

    abstract::The wild strain of Serratia marcescens rapidly degraded threonine and formed aminoacetone in a medium containing glucose and urea. Extracts of this strain showed high threonine dehydrogenase and "biosynthetic" threonine deaminase activities, but no threonine aldolase activity. Threonine dehydrogenase-deficient strain ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.135.2.318-323.1978

    authors: Komatsubara S,Murata K,Kisumi M,Chibata I

    更新日期:1978-08-01 00:00:00

  • Differential regulation of synthesis of multiple forms of specific endoglucanases by Trichoderma reesei QM9414.

    abstract::A method consisting of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and subsequent detection of endoglucanases by blotting with a polyclonal antibody against endoglucanase I was used to investigate the effect of induction and carbon catabolite derepression on the synthesis of multiple forms of endoglucana...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.8.3689-3693.1988

    authors: Messner R,Gruber F,Kubicek CP

    更新日期:1988-08-01 00:00:00

  • Sociality in Escherichia coli: Enterochelin Is a Private Good at Low Cell Density and Can Be Shared at High Cell Density.

    abstract:UNLABELLED:Many bacteria produce secreted iron chelators called siderophores, which can be shared among cells with specific siderophore uptake systems regardless of whether the cell produces siderophores. Sharing secreted products allows freeloading, where individuals use resources without bearing the cost of productio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02596-14

    authors: Scholz RL,Greenberg EP

    更新日期:2015-07-01 00:00:00

  • The Bacillus subtilis GntR family repressor YtrA responds to cell wall antibiotics.

    abstract::The transglycosylation step of cell wall synthesis is a prime antibiotic target because it is essential and specific to bacteria. Two antibiotics, ramoplanin and moenomycin, target this step by binding to the substrate lipid II and the transglycosylase enzyme, respectively. Here, we compare the ramoplanin and moenomyc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05862-11

    authors: Salzberg LI,Luo Y,Hachmann AB,Mascher T,Helmann JD

    更新日期:2011-10-01 00:00:00

  • Mismatch correction in pneumococcal transformation: donor length and hex-dependent marker efficiency.

    abstract::A hypothesis that preferential rejection of donor markers by the hex system of pneumococcus is due to lethal double-strand breaks has been examined in terms of its implications for the extent of the excision required. Experiments reported here were directed at asking whether hex-dependent marker efficiency depends on ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.125.1.125-135.1976

    authors: Guild WR,Shoemaker NB

    更新日期:1976-01-01 00:00:00