Whole-genome shotgun sequencing of Lactobacillus rhamnosus MTCC 5462, a strain with probiotic potential.

Abstract:

:Lactobacillus rhamnosus MTCC 5462 was isolated from infant gastrointestinal flora. The strain exhibited an ability to reduce cholesterol and stimulate immunity. The strain has exhibited positive results in alleviating gastrointestinal discomfort and good potential as a probiotic. We sequenced the whole genome of the strain and compared it to the published genome sequence of Lactobacillus rhamnosus GG (ATCC 53103).

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Prajapati JB,Khedkar CD,Chitra J,Suja S,Mishra V,Sreeja V,Patel RK,Ahir VB,Bhatt VD,Sajnani MR,Jakhesara SJ,Koringa PG,Joshi CG

doi

10.1128/JB.06644-11

subject

Has Abstract

pub_date

2012-03-01 00:00:00

pages

1264-5

issue

5

eissn

0021-9193

issn

1098-5530

pii

194/5/1264

journal_volume

194

pub_type

杂志文章
  • Catechol 1,2-dioxygenase from Acinetobacter calcoaceticus: purification and properties.

    abstract::Procedures for the purification of catechol 1,2-dioxygenase from extracts of Acinetobacter calcoaceticus strain ADP-96 are described. The purified enzyme was homogeneous as judged by ultracentrifugation and acrylamide gel electrophoresis. The enzyme contained 2 g-atoms of iron per mol of protein. The enzyme had a broa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.127.1.536-544.1976

    authors: Patel RN,Hou CT,Felix A,Lillard MO

    更新日期:1976-07-01 00:00:00

  • The final step of peptidoglycan subunit assembly in Escherichia coli occurs in the cytoplasm.

    abstract::The murG gene of Escherichia coli encodes the N-acetylglucosaminyltransferase responsible for the final step in the formation of the lipid-linked disaccharide-pentapeptide subunit of peptidoglycan. Using trypsin to probe maxicell spheroplasts, we show that this enzyme is peripherally associated with the inner face of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.6.1841-1843.1993

    authors: Bupp K,van Heijenoort J

    更新日期:1993-03-01 00:00:00

  • Function of alpha-D-glucosyl monophosphorylpolyprenol in biosynthesis of cell wall teichoic acids in Bacillus coagulans.

    abstract::D-[alpha-14C]]glucosyl phosphorylpolyprenol ([ 14C]Glc-P-prenol) was formed from UDP-D-[14C]glucose in each of the membrane systems obtained from Bacillus coagulans AHU 1631 and AHU 1634 and two Bacillus megaterium strains. Membranes of these B. coagulans strains, which possess beta-D-glucosyl branches on the repeatin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.5.2835-2841.1989

    authors: Shimada A,Tamatukuri J,Ito E

    更新日期:1989-05-01 00:00:00

  • Effect of temperature on Pseudomonas fluorescens chemotaxis.

    abstract::The effects of temperature and attractants on chemotaxis in psychrotrophic Pseudomonas fluorescens were examined using the Adler capillary assay technique. Several organic acids, amino acids, and uronic acids were shown to be attractants, whereas glucose and its oxidation products, gluconate and 2-ketogluconate, elici...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.143.1.338-342.1980

    authors: Lynch WH

    更新日期:1980-07-01 00:00:00

  • In vivo 31P and 13C nuclear magnetic resonance studies of acetate metabolism in Chromatium vinosum.

    abstract::31P and 13C nuclear magnetic resonance (NMR) experiments were performed on suspensions of the phototrophic bacterium Chromatium vinosum incubated anaerobically in the dark. 31P NMR spectra revealed that during prolonged dark incubation high ATP levels are maintained. This phenomenon was independent of the presence of ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.155.2.634-642.1983

    authors: Nicolay K,van Gemerden H,Hellingwerf KJ,Konings WN,Kaptein R

    更新日期:1983-08-01 00:00:00

  • A new cell surface proteinase: sequencing and analysis of the prtB gene from Lactobacillus delbruekii subsp. bulgaricus.

    abstract::Investigation of the chromosomal region downstream of the lacZ gene from Lactobacillus delbrueckii subsp. bulgaricus revealed the presence of a gene (prtB) encoding a proteinase of 1,946 residues with a predicted molecular mass of 212 kDa. The deduced amino acid sequence showed that PrtB proteinase displays significan...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.11.3059-3065.1996

    authors: Gilbert C,Atlan D,Blanc B,Portailer R,Germond JE,Lapierre L,Mollet B

    更新日期:1996-06-01 00:00:00

  • Identification, cloning, and characterization of the Ima operon, whose gene products are unique to Listeria monocytogenes.

    abstract::The lmaA gene of Listeria monocytogenes encodes a protein capable of inducing delayed-type hypersensitivity reactions in L. monocytogenes-immune mice (S. Göhmann, M. Leimeister-Wachter, E. Schiltz, W. Goebel, and T. Chakraborty, M. Microbiol. 4:1091-1099, 1990). Here we show that it is the last gene of the lma operon,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.8.2707-2716.1997

    authors: Schäferkordt S,Chakraborty T

    更新日期:1997-04-01 00:00:00

  • Chemical studies on cellular components of Bordetella pertussis. I. Purification and properties of agglutinogen.

    abstract::Onoue, Kaoru, (Kyushu University, Fukuoka, Japan), Masayasu Kitagawa, and Yuichi Yamamura. Chemical studies on cellular components of Bordetella pertussis. I. Purification and properties of agglutinogen. J. Bacteriol. 82:648-656. 1961.-A method is described for the preparation of the agglutinogen of Hemophilus pertuss...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.82.5.648-656.1961

    authors: ONOUE K,KITAGAWA M,YAMAMURA Y

    更新日期:1961-11-01 00:00:00

  • Shuttle mutagenesis of Neisseria gonorrhoeae: pilin null mutations lower DNA transformation competence.

    abstract::The method of shuttle mutagenesis has been extended to Neisseria gonorrhoeae. We have constructed a defective mini-Tn3 derivative that encodes chloramphenicol resistance in both N. gonorrhoeae and Escherichia coli and selected for mutations in the chloramphenicol resistance gene that express higher levels of antibioti...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.1.40-46.1990

    authors: Seifert HS,Ajioka RS,Paruchuri D,Heffron F,So M

    更新日期:1990-01-01 00:00:00

  • Positive selection for loss-of-function tat mutations identifies critical residues required for TatA activity.

    abstract::The Tat system, found in the cytoplasmic membrane of many bacteria, is a general export pathway for folded proteins. Here we describe the development of a method, based on the transport of chloramphenicol acetyltransferase, that allows positive selection of mutants defective in Tat function. We have demonstrated the u...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.8.2920-2925.2005

    authors: Hicks MG,Lee PA,Georgiou G,Berks BC,Palmer T

    更新日期:2005-04-01 00:00:00

  • Dynamics of FtsZ assembly during sporulation in Streptomyces coelicolor A3(2).

    abstract::FtsZ, the bacterial tubulin homologue, is the main player in at least two distinct processes of cell division during the development of Streptomyces coelicolor A3(2). It forms cytokinetic rings and is required for the formation of both the widely spaced hyphal cross walls in the substrate mycelium and the specialized ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.9.3227-3237.2005

    authors: Grantcharova N,Lustig U,Flärdh K

    更新日期:2005-05-01 00:00:00

  • Cell-cycle-specific oscillation in the composition of chromatophore membrane in Rhodospirillum rubrum.

    abstract::Synchrony in phototrophic cultures of Rhodospirillum rubrum was induced by stationary-phase cycling or by alterations in light intensity. Intracytoplasmic chromatophore membranes were prepared by differential centrifugation. Analysis of the composition of chromatophores obtained from cells at different times indicated...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.166.3.818-823.1986

    authors: Myers CR,Collins ML

    更新日期:1986-06-01 00:00:00

  • Molecular cloning of genes involved with expression of A-band lipopolysaccharide, an antigenically conserved form, in Pseudomonas aeruginosa.

    abstract::Most strains of Pseudomonas aeruginosa can express two chemically and immunologically distinct types of lipopolysaccharide (LPS), an antigenically conserved form called A band and the serotype-specific form called B band. To study the molecular controls regulating expression of the A-band LPS antigen, we have cloned t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.18.5624-5630.1991

    authors: Lightfoot J,Lam JS

    更新日期:1991-09-01 00:00:00

  • Biogenesis of the fraction 1 capsule and analysis of the ultrastructure of Yersinia pestis.

    abstract::Analysis of a Yersinia pestis Delta caf1A mutant demonstrated that the Caf1A usher is required for the assembly and secretion of the fraction 1 capsule. The capsule assembled into thin fibrils and denser aggregates on the bacterial surface. Pilus-like fibers were also detected on the surface of Y. pestis. The capsule ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01840-07

    authors: Runco LM,Myrczek S,Bliska JB,Thanassi DG

    更新日期:2008-05-01 00:00:00

  • Global gene expression analysis of the heat shock response in the phytopathogen Xylella fastidiosa.

    abstract::Xylella fastidiosa is a phytopathogenic bacterium that is responsible for diseases in many economically important crops. Although different strains have been studied, little is known about X. fastidiosa stress responses. One of the better characterized stress responses in bacteria is the heat shock response, which ind...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00182-06

    authors: Koide T,Vêncio RZ,Gomes SL

    更新日期:2006-08-01 00:00:00

  • Importance of anaerobic superoxide dismutase synthesis in facilitating outgrowth of Escherichia coli upon entry into an aerobic habitat.

    abstract::The manganese-containing isozyme of superoxide dismutase (MnSOD) is synthesized by Escherichia coli only during aerobiosis, in accordance with the fact that superoxide can be formed only in aerobic environments. In contrast, E. coli continues to synthesize the iron-containing isozyme (FeSOD) even in the absence of oxy...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.24.7653-7658.1994

    authors: Kargalioglu Y,Imlay JA

    更新日期:1994-12-01 00:00:00

  • A Ti plasmid-encoded enzyme required for degradation of mannopine is functionally homologous to the T-region-encoded enzyme required for synthesis of this opine in crown gall tumors.

    abstract::The mocC gene encoded by the octopine/mannityl opine-type Ti plasmid pTi15955 is related at the nucleotide sequence level to mas1' encoded by the T region of this plasmid. While Mas1 is required for the synthesis of mannopine (MOP) by crown gall tumor cells, MocC is essential for the utilization of MOP by Agrobacteriu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.11.3285-3292.1996

    authors: Kim KS,Chilton WS,Farrand SK

    更新日期:1996-06-01 00:00:00

  • Genetics of sulfate transport by Salmonella typhimurium.

    abstract::Sixty-four mutants were isolated from the LT-2 wild-type strain of Salmonella typhimurium by selecting for chromate resistance. The majority of lesions were shown to lie in the cysA gene. (i) The mutants cannot take up sulfate, a finding which verifies the role of cysA in sulfate transport. In addition, 52 sulfate-tra...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.105.3.1053-1062.1971

    authors: Ota N,Galsworthy PR,Pardee AB

    更新日期:1971-03-01 00:00:00

  • Effects of oxygen and heme on the development of a microbial respiratory system.

    abstract::The effect of adding hemin to anaerobically grown cells of a strain of Staphylococcus epidermidis, which was heme-deficient due to anaerobic growth, has been examined. Cells grown anaerobically in media containing hemin exhibited a marked increase in several oxidative activities as compared with cells grown anaerobica...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.93.1.278-285.1967

    authors: Jacobs NJ,Maclosky ER,Conti SF

    更新日期:1967-01-01 00:00:00

  • Genetic analysis of the tetA(C) gene on plasmid pBR322.

    abstract::The TetA(C) protein, encoded by the tetA(C) gene of plasmid pBR322, is a member of a family of membrane-bound proteins that mediate energy-dependent efflux of tetracycline from the bacterial cell. The tetA(C) gene was mutagenized with hydroxylamine, and missense mutations causing the loss of tetracycline resistance we...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.24.7926-7933.1992

    authors: McNicholas P,Chopra I,Rothstein DM

    更新日期:1992-12-01 00:00:00

  • Molecular cloning and sequencing of the gene encoding the fimbrial subunit protein of Bacteroides gingivalis.

    abstract::The gene encoding the fimbrial subunit protein of Bacteroides gingivalis 381, fimbrilin, has been cloned and sequenced. The gene was present as a single copy on the bacterial chromosome, and the codon usage in the gene conformed closely to that expected for an abundant protein. The predicted size of the mature protein...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.4.1658-1665.1988

    authors: Dickinson DP,Kubiniec MA,Yoshimura F,Genco RJ

    更新日期:1988-04-01 00:00:00

  • Vfr Directly Activates exsA Transcription To Regulate Expression of the Pseudomonas aeruginosa Type III Secretion System.

    abstract:UNLABELLED:The Pseudomonas aeruginosa cyclic AMP (cAMP)-Vfr system (CVS) is a global regulator of virulence gene expression. Regulatory targets include type IV pili, secreted proteases, and the type III secretion system (T3SS). The mechanism by which CVS regulates T3SS gene expression remains undefined. Single-cell exp...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00049-16

    authors: Marsden AE,Intile PJ,Schulmeyer KH,Simmons-Patterson ER,Urbanowski ML,Wolfgang MC,Yahr TL

    更新日期:2016-04-14 00:00:00

  • Localization of the Escherichia coli rnt gene encoding RNase T by using a combination of physical and genetic mapping.

    abstract::The rnt gene encoding RNase T was cloned on a 13-kilobase BamHI fragment. Restriction analysis of the fragment and comparison of it with the Escherichia coli restriction map localized rnt to kilobase coordinates 1733 to 1746, corresponding to about 36 min on the genetic map. The map location was confirmed by cotransdu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.10.5736-5737.1989

    authors: Case LM,Chen XN,Deutscher MP

    更新日期:1989-10-01 00:00:00

  • Use of temperature-sensitive mutants to study gene expression during sporulation in Bacillus subtilis.

    abstract::Two temperature-sensitive sporulation mutants have been characterized. One mutant, which is blocked at stage II, has a short temperature-sensitive period that occurs at about the time when the spore septum is formed. Cells can escape the sporulation block, if incubated for a short period at the permissive temperature,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.126.2.928-936.1976

    authors: Young M

    更新日期:1976-05-01 00:00:00

  • Two-dimensional restriction analysis of the Bacillus subtilis genome: gene purification and ribosomal ribonucleic acid gene organization.

    abstract::With two-dimensional restriction enzyme analysis we have been able to cleave the Bacillus subtilis genome and resolve the resulting deoxyribonucleic acid (DNA) segments into discrete bands on agarose gels. A general procedure for gene purification has been developed by coupling multidimensional restriction analysis wi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.129.1.492-500.1977

    authors: Potter SS,Bott KF,Newbold JE

    更新日期:1977-01-01 00:00:00

  • Phenotypic switching of Thiobacillus ferrooxidans.

    abstract::Two solid medium formulations, designated 100:10 and 10:10, were developed for the growth of Thiobacillus ferrooxidans. The new media contain a mixture of both ferrous iron and thiosulfate as available energy sources, permitting the detection of colony morphology variants that arise spontaneously in a wild-type popula...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.9.3915-3923.1988

    authors: Schrader JA,Holmes DS

    更新日期:1988-09-01 00:00:00

  • Unraveling the function of the Rhodospirillum rubrum activator of polyhydroxybutyrate (PHB) degradation: the activator is a PHB-granule-bound protein (phasin).

    abstract::Efficient hydrolysis of native poly(3-hydroxybutyrate) (nPHB) granules in vitro by soluble PHB depolymerase of Rhodospirillum rubrum requires pretreatment of nPHB with an activator compound present in R. rubrum cells (J. M. Merrick and M. Doudoroff, J. Bacteriol. 88:60-71, 1964). Edman sequencing of the purified activ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.8.2466-2475.2004

    authors: Handrick R,Reinhardt S,Schultheiss D,Reichart T,Schüler D,Jendrossek V,Jendrossek D

    更新日期:2004-04-01 00:00:00

  • Biosynthesis of phospholipids in Bacillus megaterium.

    abstract::Information on the biosynthesis of phospholipids in bacteria has been derived principally from the study of Escherichia coli and other gram-negative organisms. We have now carried out a detailed study of the pathways of phospholipid biosynthesis in the gram-positive organism Bacillus megarterium KM in relation to inve...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.140.3.996-1007.1979

    authors: Langley KE,Yaffe MP,Kennedy EP

    更新日期:1979-12-01 00:00:00

  • Transport of glutamine by Streptococcus bovis and conversion of glutamine to pyroglutamic acid and ammonia.

    abstract::Streptococcus bovis JB1 cells energized with glucose transported glutamine at a rate of 7 nmol/mg of protein per min at a pH of 5.0 to 7.5; sodium had little effect on the transport rate. Because valinomycin-treated cells loaded with K and diluted into Na (pH 6.5) to create an artificial delta psi took up little gluta...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.6.2981-2985.1989

    authors: Chen GJ,Russell JB

    更新日期:1989-06-01 00:00:00

  • Functional genomics enables identification of genes of the arginine transaminase pathway in Pseudomonas aeruginosa.

    abstract::Arginine utilization in Pseudomonas aeruginosa with multiple catabolic pathways represents one of the best examples of the metabolic versatility of this organism. To identify genes involved in arginine catabolism, we have employed DNA microarrays to analyze the transcriptional profiles of this organism in response to ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00261-07

    authors: Yang Z,Lu CD

    更新日期:2007-06-01 00:00:00