Evidence for specific inhibition of translocation of aminoacyl-transfer ribonucleic acid by the pretreatment of ribosomes of Bacillus subtilis with p-chloromercuribenzoate.

abstract：:Nonpiliated, phage phi 6-resistant mutants of Pseudomonas syringae pv. phaseolicola were generated by Tn5 transposon mutagenesis. A P. syringae pv. phaseolicola LR700 cosmid library was screened with Tn5-containing EcoRI fragments cloned from nonpiliated mutants. The cosmid clone pVK253 complemented the nonpiliated mu...

journal_title：Journal of bacteriology

authors： Roine E,Nunn DN,Paulin L,Romantschuk M

更新日期：1996-01-01 00:00:00

abstract：:Expression of the Vibrio vulnificus metalloprotease gene, vvp, was turned up rapidly when bacterial growth reached the late log phase. A similar pattern of expression has been found in the metalloprotease gene of Vibrio cholerae, and this has been shown to be regulated by a Vibrio harveyi LuxR-like transcriptional act...

journal_title：Journal of bacteriology

authors： Shao CP,Hor LI

更新日期：2001-02-01 00:00:00

abstract：UNLABELLED:The gene encoding a nonoxidative decarboxylase capable of catalyzing the transformation of 2-hydroxy-1-naphthoic acid (2H1NA) to 2-naphthol was identified, recombinantly expressed, and purified to homogeneity. The putative gene sequence of the decarboxylase (hndA) encodes a 316-amino-acid protein (HndA) with...

journal_title：Journal of bacteriology

authors： Pal Chowdhury P,Basu S,Dutta A,Dutta TK

更新日期：2016-05-27 00:00:00

abstract：:A bacterium was isolated by elective culture with p-hydroxybenzoate as substrate and nitrate as electron acceptor. It grew either aerobically or anaerobically, by nitrate respiration, on a range of aromatic compounds. The organism was identified as a pseudomonad and was given the trivial name Pseudomonas PN-1. Benzoat...

journal_title：Journal of bacteriology

authors： Taylor BF,Campbell WL,Chinoy I

更新日期：1970-05-01 00:00:00

abstract：:After the Bacillus subtilis nucleoid was dissected with restriction endonucleases, a specific DNA sequence from the purA region was isolated in a particulate form that probably originated from the cell membrane. Precise definition of the binding region within this sequence was achieved by a novel procedure based on a ...

journal_title：Journal of bacteriology

authors： Sargent MG,Bennett MF

更新日期：1986-04-01 00:00:00

abstract：:Piliated, competent gonococci are known to preferentially take up homologous transforming DNA into the cell. We examined the mechanism for DNA uptake with pFA10, a hybrid 11.5-kilobase (kb) penicillin-resistant (Pcr) plasmid composed of heterologous DNA from a 7.2-kb Pcr plasmid and homologous DNA from a 4.2-kb gonoco...

journal_title：Journal of bacteriology

authors： Graves JF,Biswas GD,Sparling PF

更新日期：1982-12-01 00:00:00

abstract：:Systematic inactivation of pathways involved in DNA alkylation damage repair demonstrated that inactivation of the ada, ogt, tag, uvrA, and mfd genes is required to detect a Salmonella enterica virulence decrease. Furthermore, the fitness of S. enterica, defective in these genes, is lowered only when the bacterium is ...

journal_title：Journal of bacteriology

authors： Alvarez G,Campoy S,Spricigo DA,Teixidó L,Cortés P,Barbé J

更新日期：2010-04-01 00:00:00

abstract：:Desulfovibrio vulgaris Hildenborough, a sulfate-reducing bacterium classified as an obligate anaerobe, swam to a preferred oxygen concentration of 0.02 to 0.04% (0.24 to 0.48 microM), a level which also supported growth. Oxygen concentrations of 0.08% and higher arrested growth. We propose that in zones of transition ...

journal_title：Journal of bacteriology

authors： Johnson MS,Zhulin IB,Gapuzan ME,Taylor BL

更新日期：1997-09-01 00:00:00

abstract：:DNA damage and perturbations in DNA replication can induce global transcriptional responses that can help organisms repair the damage and survive. RecA is known to mediate transcriptional responses to DNA damage in several bacterial species by inactivating the repressor LexA and phage repressors. To gain insight into ...

journal_title：Journal of bacteriology

authors： Goranov AI,Kuester-Schoeck E,Wang JD,Grossman AD

更新日期：2006-08-01 00:00:00

abstract：:2-Carboxybenzaldehyde dehydrogenase from the phenanthrene-degrading bacterium Nocardioides sp. strain KP7 was purified and characterized. The purified enzyme had a molecular mass of 53 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 205 kDa by gel filtration chromatography. Thus, the homotetramer ...

journal_title：Journal of bacteriology

authors： Iwabuchi T,Harayama S

更新日期：1997-10-01 00:00:00

abstract：:Supplementation of the growth medium with high concentrations of NaCl, KCl, or sucrose caused a drastic change in the ratio of the two peptidoglycan-associated major outer membrane proteins of Escherichia coli K-12 in that the amounts of proteins b and c present in cell envelope preparations decreased and increased, r...

journal_title：Journal of bacteriology

authors： Alphen WV,Lugtenberg B

更新日期：1977-08-01 00:00:00

abstract：:Borrelia burgdorferi, the Lyme disease agent, and other members of the spirochetal genus Borrelia have double-stranded linear plasmids in addition to supercoiled circular plasmids. The copy number relative to the chromosome was determined for 49- and 16-kb linear plasmids and a 27-kb circular plasmid of the type strai...

journal_title：Journal of bacteriology

authors： Hinnebusch J,Barbour AG

更新日期：1992-08-01 00:00:00

abstract：:gldA, the structural gene for the NAD(+)-dependent glycerol dehydrogenase, was mapped at 89.2 min on the Escherichia coli linkage map, cotransducible with, but not adjacent to, the glpFKX operon encoding the proteins for the uptake and phosphorylation of glycerol. gldA was cloned, and its position on the physical map ...

journal_title：Journal of bacteriology

authors： Truniger V,Boos W

更新日期：1994-03-01 00:00:00

abstract：:The crystal structure of Escherichia coli PdxY, the protein product of the pdxY gene, has been determined to a 2.2-A resolution. PdxY is a member of the ribokinase superfamily of enzymes and has sequence homology with pyridoxal kinases that phosphorylate pyridoxal at the C-5' hydroxyl. The protein is a homodimer with ...

journal_title：Journal of bacteriology

authors： Safo MK,Musayev FN,Hunt S,di Salvo ML,Scarsdale N,Schirch V

更新日期：2004-12-01 00:00:00

abstract：:The levels of reduced and oxidized nicotinamide adenine dinucleotides were determined in Xanthobacter flavus during a transition from heterotrophic to autotrophic growth. Excess reducing equivalents are rapidly dissipated following induction of the Calvin cycle, indicating that the Calvin cycle serves as a sink for ex...

journal_title：Journal of bacteriology

authors： van Keulen G,Dijkhuizen L,Meijer WG

更新日期：2000-08-01 00:00:00

abstract：:The stator of the sodium-driven flagellar motor of Vibrio alginolyticus is a membrane protein complex composed of four PomA and two PomB subunits. PomB has a peptidoglycan-binding motif in the C-terminal region. In this study, four kinds of PomB deletions in the C terminus were constructed. None of the deletion protei...

journal_title：Journal of bacteriology

authors： Yakushi T,Hattori N,Homma M

更新日期：2005-01-01 00:00:00

abstract：:Cell suspensions of Bacteroides fragilis were allowed to ferment glucose and lactate labeled with (14)C in different positions. The fermentation products, propionate and acetate, were isolated, and the distribution of radioactivity was determined. An analysis of key enzymes of possible pathways was also made. The resu...

journal_title：Journal of bacteriology

authors： Macy JM,Ljungdahl LG,Gottschalk G

更新日期：1978-04-01 00:00:00

abstract：:PhoB is the response regulator of the Pho regulon. It is composed of two distinct domains, an N-terminal receiver domain and a C-terminal output domain that binds DNA and interacts with sigma(70) to activate transcription of the Pho regulon. Phosphorylation of the receiver domain is required for activation of the prot...

journal_title：Journal of bacteriology

authors： Ellison DW,McCleary WR

更新日期：2000-12-01 00:00:00

abstract：:The YycFG two-component system is the only signal transduction system in Bacillus subtilis known to be essential for cell viability. This system is highly conserved in low-G+C gram-positive bacteria, regulating important processes such as cell wall homeostasis, cell membrane integrity, and cell division. Four other ge...

journal_title：Journal of bacteriology

authors： Szurmant H,Mohan MA,Imus PM,Hoch JA

更新日期：2007-04-01 00:00:00

abstract：:The occurrence of the novel regulatory nucleotide bis(3',5')-cyclic diguanylic acid (c-di-GMP) and its relation to cellulose biogenesis in the plant pathogen Agrobacterium tumefaciens was studied. c-di-GMP was detected in acid extracts of 32P-labeled cells grown in various media, and an enzyme responsible for its form...

journal_title：Journal of bacteriology

authors： Amikam D,Benziman M

更新日期：1989-12-01 00:00:00

abstract：:Circulating endotoxin was specifically precipitated from plasma samples withdrawn from three different animal species subsequent to parenteral injection of the toxin. Lipoprotein-positive staining and esterase activity were demonstrated on the precipitation lines formed in immunodiffusion, thus establishing the in viv...

journal_title：Journal of bacteriology

authors： Skarnes RC

更新日期：1968-06-01 00:00:00

abstract：:The mukB gene is essential for the partitioning of sister chromosomes in Escherichia coli. A mukB null mutant is hypersensitive to the DNA gyrase inhibitor novobiocin. In this work, we isolated mutants suppressing the novobiocin hypersensitivity of the mukB null mutation. All suppressor mutations are localized in or n...

journal_title：Journal of bacteriology

authors： Adachi S,Hiraga S

更新日期：2003-07-01 00:00:00

abstract：:Further studies on the expression of the two aspartokinase activities in Bacillus bovis are presented. Aspartokinase I (previously shown to be inhibited and repressed by lysine) was found to be repressed by diaminopimelate in the wild-type strain. However, in a mutant unable to convert diaminopimelate to lysine, starv...

journal_title：Journal of bacteriology

authors： Hitchcock MJ,Hodgson B,Linforth JL

更新日期：1980-05-01 00:00:00

abstract：:A collection of 169 strains, including 91 obtained from cases of gastroenteritis and 41 from localized tissue infections and infections of the eye and ear, was submitted to an extensive nutritional, physiological, and morphological characterization. The nutritional and physiological data obtained from these strains, a...

journal_title：Journal of bacteriology

authors： Baumann P,Baumann L,Reichelt JL

更新日期：1973-03-01 00:00:00

abstract：:Sites of transposon Tn7 insertion in the Escherichia coli chromosome were examined, and two distinct classes of target sites differing in nucleotide sequence were identified. The target site choice was found to be determined by Tn7-encoded transposition genes. ...

journal_title：Journal of bacteriology

authors： Kubo KM,Craig NL

更新日期：1990-05-01 00:00:00

abstract：:We report a search for small RNAs (sRNAs) in the low-GC, gram-positive human pathogen Streptococcus pneumoniae. Based on bioinformatic analyses by Livny et al. (J. Livny, A. Brencic, S. Lory, and M. K. Waldor, Nucleic Acids Res. 34:3484-3493, 2006), we tested 40 candidates by Northern blotting and confirmed the expres...

journal_title：Journal of bacteriology

authors： Tsui HC,Mukherjee D,Ray VA,Sham LT,Feig AL,Winkler ME

更新日期：2010-01-01 00:00:00

abstract：:Campylobacter jejuni produces both lipooligosaccharide (LOS) and a higher-molecular-weight polysaccharide that is believed to form a capsule. The role of these surface polysaccharides in C. jejuni-mediated enteric disease is unclear; however, epitopes associated with the LOS are linked to the development of neurologic...

journal_title：Journal of bacteriology

authors： Oldfield NJ,Moran AP,Millar LA,Prendergast MM,Ketley JM

更新日期：2002-04-01 00:00:00

abstract：:Several mycobacterial strains, such as Mycobacterium flavescens, Mycobacterium gastri, Mycobacterium neoaurum, Mycobacterium parafortuitum, Mycobacterium peregrinum, Mycobacterium phlei, Mycobacterium smegmatis, Mycobacterium tuberculosis, and Mycobacterium vaccae, were found to grow on carbon monoxide (CO) as the sol...

journal_title：Journal of bacteriology

authors： Park SW,Hwang EH,Park H,Kim JA,Heo J,Lee KH,Song T,Kim E,Ro YT,Kim SW,Kim YM

更新日期：2003-01-01 00:00:00

abstract：:The transferred DNA (T-DNA) is transported from Agrobacterium tumefaciens to the nucleus and is stably integrated into the genome of many plant species. It has been proposed that the VirD2 protein, tightly attached to the T-DNA, pilots the T-DNA into the plant cell nucleus and that it is involved in integration. Using...

journal_title：Journal of bacteriology

authors： Koukolíková-Nicola Z,Raineri D,Stephens K,Ramos C,Tinland B,Nester EW,Hohn B

更新日期：1993-02-01 00:00:00

abstract：:Four mutants specifically deficient in the activity of isocitrate lyase were independently isolated in the alkane yeast Saccharomycopsis lipolytica. Genetic analysis by means of protoplast fusion and mitotic haploidization revealed that the mutations were recessive and non-complementary at a single genetic locus, icl....

journal_title：Journal of bacteriology

authors： Matsuoka M,Himeno T,Aiba S

更新日期：1984-03-01 00:00:00