Biochemical and genetic characterization of 2-carboxybenzaldehyde dehydrogenase, an enzyme involved in phenanthrene degradation by Nocardioides sp. strain KP7.

abstract：:The gene responsible for the uncommon glutamate dehydrogenase (GDH) activity of Lactococcus lactis was identified and characterized. It encodes a GDH of family I that is mainly active in glutamate biosynthesis, is carried by a large plasmid, and is included, with functional cadmium resistance genes, in a remnant Tn3-l...

journal_title：Journal of bacteriology

authors： Tanous C,Chambellon E,Sepulchre AM,Yvon M

更新日期：2005-07-01 00:00:00

abstract：:Previous determination of the nucleotide sequence of the iol region of the Bacillus subtilis genome allowed us to predict the structure of the iol operon for myo-inositol catabolism, consisting of 10 iol genes (iolA to iouJ); iolG corresponds to idh, encoding myo-inositol 2-dehydrogenase (Idh). Primer extension analys...

journal_title：Journal of bacteriology

authors： Yoshida KI,Aoyama D,Ishio I,Shibayama T,Fujita Y

更新日期：1997-07-01 00:00:00

abstract：:The nucleotide sequence of a region located downstream of the nifB gene, both in the cyanobacterium Anabaena sp. strain PCC 7120 and in Rhizobium meliloti, has been determined. This region contains a gene (fdxN) whose predicted polypeptide product strongly resembles typical bacterial ferredoxins. Cyanobacteria have no...

journal_title：Journal of bacteriology

authors： Mulligan ME,Buikema WJ,Haselkorn R

更新日期：1988-09-01 00:00:00

abstract：:Wzc(CPS) is a tyrosine autokinase essential for the assembly of a high-molecular-weight (HMW) group 1 capsular polysaccharide (CPS) in Escherichia coli. Homologues of Wzc participate in the formation of CPS and exopolysaccharides in a variety of gram-positive and gram-negative bacteria. Phosphorylation of tyrosine res...

journal_title：Journal of bacteriology

authors： Paiment A,Hocking J,Whitfield C

更新日期：2002-12-01 00:00:00

abstract：:The first step in the transfer of the Bacteroides conjugative transposon CTnDOT is excision of the integrated element from the chromosome to form a circular transfer intermediate. Excision occurs only after the bacteria are exposed to tetracycline. Previously, four excision genes were identified. One was the integrase...

journal_title：Journal of bacteriology

authors： Moon K,Shoemaker NB,Gardner JF,Salyers AA

更新日期：2005-08-01 00:00:00

abstract：:Rhodospirillum rubrum was shown to grow fermentatively on fructose with N2 as a nitrogen source. The nitrogenase activity of these cells was regulated by the NH4+ switch-off/switch-on mechanism in a manner identical to that for photosynthetically grown cells. In vitro, the inactive nitrogenase Fe protein from fermenti...

journal_title：Journal of bacteriology

authors： Schultz JE,Gotto JW,Weaver PF,Yoch DC

更新日期：1985-06-01 00:00:00

abstract：:A mutator mutation of Salmonella typhimurium, observed in a strain resistant to P22 and rifampin, affects mutation rates of bacterial, R factor, and prophage P22 genes. ...

journal_title：Journal of bacteriology

authors： Guterman SK,Wright A

更新日期：1974-08-01 00:00:00

abstract：:The multifunctional 6-methylsalicylic acid synthase gene from Penicillium patulum was engineered for regulated expression in Streptomyces coelicolor. Production of significant amounts of 6-methylsalicylic acid by the recombinant strain was proven by nuclear magnetic resonance spectroscopy. These results suggest that i...

journal_title：Journal of bacteriology

authors： Bedford DJ,Schweizer E,Hopwood DA,Khosla C

更新日期：1995-08-01 00:00:00

abstract：:Bile acids are surface-active steroid compounds with toxic effects for bacteria. Recently, the isolation and characterization of a bacterium, Pseudomonas sp. strain Chol1, growing with bile acids as the carbon and energy source was reported. In this study, initial reactions of the aerobic degradation pathway for the b...

journal_title：Journal of bacteriology

authors： Birkenmaier A,Holert J,Erdbrink H,Moeller HM,Friemel A,Schoenenberger R,Suter MJ,Klebensberger J,Philipp B

更新日期：2007-10-01 00:00:00

abstract：:Here we show that the type III secretion gatekeeper protein SepL resembles an aberrant effector protein in binding to a class 1 type III secretion chaperone (Orf12, here renamed CesL). We also show that short N-terminal fragments (≤70 amino acids) from SepL are capable of targeting fusion proteins for secretion and tr...

journal_title：Journal of bacteriology

authors： Younis R,Bingle LE,Rollauer S,Munera D,Busby SJ,Johnson S,Deane JE,Lea SM,Frankel G,Pallen MJ

更新日期：2010-11-01 00:00:00

abstract：:We isolated five new temperature-sensitive alleles of the essential cell division gene ftsZ in Escherichia coli, using P1-mediated, localized mutagenesis. The five resulting single amino acid changes (Gly109-->Ser109 for ftsZ6460, Ala129-->Thr129 for ftsZ972, Val157-->Met157 for ftsZ2066, Pro203-->Leu203 for ftsZ9124,...

journal_title：Journal of bacteriology

authors： Addinall SG,Small E,Whitaker D,Sturrock S,Donachie WD,Khattar MM

更新日期：2005-01-01 00:00:00

abstract：:The general stress response mediated by the sigma factor RpoS is important for survival of bacteria in adverse environments. A mutant unable to produce RpoS was constructed using the diazotrophic bacterium Azotobacter vinelandii strain UW. Under nondesiccating, solid-medium growth conditions the wild type was culturab...

journal_title：Journal of bacteriology

authors： Sandercock JR,Page WJ

更新日期：2008-02-01 00:00:00

abstract：:Listeria monocytogenes has caused numerous human outbreaks. Here we report draft genomes of L. monocytogenes J1816 and J1-220, which belong to epidemic clones II and IV, respectively. Whole-genome sequence analysis of these strains provides a tool for studying the short-term evolution of these epidemic clones. ...

journal_title：Journal of bacteriology

authors： Chen Y,Strain EA,Allard M,Brown EW

更新日期：2011-07-01 00:00:00

abstract：:Martin, Alice J. (Nassau County Department of Health, Hempstead, N.Y.), and Margaret O'Brien. Detection of enteropathogenic Escherichia coli in fecal cultures by use of a modified fluorescent-antibody technique. J. Bacteriol. 89:570-573. 1965.-The application of the fluorescent-antibody (FA) technique to cultures of f...

journal_title：Journal of bacteriology

authors： MARTIN AJ,O'BRIEN M

更新日期：1965-03-01 00:00:00

abstract：:Lactobacillus rhamnosus MTCC 5462 was isolated from infant gastrointestinal flora. The strain exhibited an ability to reduce cholesterol and stimulate immunity. The strain has exhibited positive results in alleviating gastrointestinal discomfort and good potential as a probiotic. We sequenced the whole genome of the s...

journal_title：Journal of bacteriology

authors： Prajapati JB,Khedkar CD,Chitra J,Suja S,Mishra V,Sreeja V,Patel RK,Ahir VB,Bhatt VD,Sajnani MR,Jakhesara SJ,Koringa PG,Joshi CG

更新日期：2012-03-01 00:00:00

abstract：:The essential function of penicillin-binding protein 2 (PBP2) in methicillin-susceptible Staphylococcus aureus RN4220 was clearly established by placing the pbp2 gene under control of the inducible P(spac) promoter; the resulting bacteria were unable to grow in the absence of inducer. In contrast, the deficit in PBP2 ...

journal_title：Journal of bacteriology

authors： Pinho MG,Filipe SR,de Lencastre H,Tomasz A

更新日期：2001-11-01 00:00:00

abstract：:Studies with methionineless strains of Escherichia coli WWU and Bacillus megaterium KM have shown that selenomethionine only partially satisfies their methionine requirement. ...

journal_title：Journal of bacteriology

authors： Wu M,Wachsman JT

更新日期：1970-12-01 00:00:00

abstract：:The acetogenic bacterium Clostridium thermoautotrophicum, grown on methanol, glucose, or CO2-H2, contained high levels of corrinoids, formate dehydrogenase, tetrahydrofolate enzymes, carbon monoxide dehydrogenase, and hydrogenase. Cell-free extracts catalyzed pyruvate-dependent formation of acetate from methyltetrahyd...

journal_title：Journal of bacteriology

authors： Clark JE,Ragsdale SW,Ljungdahl LG,Wiegel J

更新日期：1982-07-01 00:00:00

abstract：:Chromatin immunoprecipitation and microarray (ChIP-chip) analysis showed that the nitric oxide (NO)-sensitive repressor NsrR from Escherichia coli binds in vivo to the promoters of the tynA and feaB genes. These genes encode the first two enzymes of a pathway that is required for the catabolism of phenylethylamine (PE...

journal_title：Journal of bacteriology

authors： Rankin LD,Bodenmiller DM,Partridge JD,Nishino SF,Spain JC,Spiro S

更新日期：2008-09-01 00:00:00

abstract：:Deoxycytosine methylase (Dcm) enzyme activity causes mutagenesis in vitro either directly by enzyme-induced deamination of cytosine to uracil in the absence of the methyl donor, S-adenosylmethionine (SAM), or indirectly through spontaneous deamination of [5-methyl]cytosine to thymine. Using a Lac reversion assay, we i...

journal_title：Journal of bacteriology

authors： Macintyre G,Atwood CV,Cupples CG

更新日期：2001-02-01 00:00:00

abstract：:Amber mutants of bacteriophage P1 were used to identify functions involved in late regulation of the P1 lytic growth cycle. A single function has been genetically identified to be involved in activation of the phage-specific late promoter sequence Ps. In vivo, P1 gene 10 amber mutants fail to trans activate a lacZ ope...

journal_title：Journal of bacteriology

authors： Lehnherr H,Guidolin A,Arber W

更新日期：1991-10-01 00:00:00

abstract：:The Bacillus subtilis sacY and sacT genes encode antiterminator proteins, similar to the Escherichia coli bglG gene product and required for transcription of sucrose metabolism genes. A Tn10 insertion into bglP (formerly sytA) has been previously identified as restoring sucrose utilization to a strain with deletions o...

journal_title：Journal of bacteriology

authors： Le Coq D,Lindner C,Krüger S,Steinmetz M,Stülke J

更新日期：1995-03-01 00:00:00

abstract：:Manganese is accumulated in Bacillus subtilis by a highly specific active transport system. This trace element "pump" is insensitive to added magnesium or calcium and preferentially accumulates manganese in the presence of cobalt, iron, and copper. Manganese uptake in B. subtilis is inhibited by cyanide, azide, pentac...

journal_title：Journal of bacteriology

authors： Eisenstadt E,Fisher S,Der CL,Silver S

更新日期：1973-03-01 00:00:00

abstract：:Strains of Escherichia coli K12 have been prepared that carry the ruv mutation in combination with lon or uvrA mutant alleles. The ruv minus uvrA minus double mutant is more sensitive to ultraviolet light than the urvA minus single mutant, whereas the strain with ruv and ion mutations shows the same sensitivity to ult...

journal_title：Journal of bacteriology

authors： Iyehara H,Otsuji N

更新日期：1975-02-01 00:00:00

abstract：:When allyl alcohol was used as a suicide substrate, spontaneous mutants and UV light- and nitrous acid-generated mutants of Methylobacterium organophilum XX were selected which grew on methylamine but not on methanol. There was no detectable methanol dehydrogenase (MDH) activity in crude extracts of these mutants, yet...

journal_title：Journal of bacteriology

authors： Machlin SM,Tam PE,Bastien CA,Hanson RS

更新日期：1988-01-01 00:00:00

abstract：:Some strains of Streptomyces produce sialidases. Two sialidases were purified over 1,000-fold from a culture filtrate of two Streptomyces species. They had the same properties in molecular weight, behavior to ions and other reagents, and substrate specificity. They showed very small differences in kinetic properties, ...

journal_title：Journal of bacteriology

authors： Kunimoto S,Aoyagi T,Takeuchi T,Umezawa H

更新日期：1974-08-01 00:00:00

abstract：:Relatedness in species of Erwinia was assessed by determining the extent of reassociation in heterologous deoxyribonucleic acid preparations. Thermal elution chromatography on hydroxyapatite was used to separate reassociated nucleotide sequences from nonreassociated sequences and to determine the thermal stability of ...

journal_title：Journal of bacteriology

authors： Brenner DJ,Fanning GR,Steigerwalt AG

更新日期：1972-04-01 00:00:00

abstract：:The region of the Rhizobium leguminosarum biovar viciae Sym plasmid pRL1JI, responsible for the production and secretion of a previously described 50-kilodalton protein (R. A. de Maagd, C. A. Wijffelman, E. Pees, and B. J. J. Lugtenberg, J. Bacteriol. 170:4424-4427, 1988), was cloned and its nucleotide sequence was de...

journal_title：Journal of bacteriology

authors： de Maagd RA,Wijfjes AH,Spaink HP,Ruiz-Sainz JE,Wijffelman CA,Okker RJ,Lugtenberg BJ

更新日期：1989-12-01 00:00:00

abstract：:A ubiquinone-deficient mutant, carrying mutations in two genes affecting ubiquinone biosynthesis, has been used, in comparison with a normal strain, to determine the sequence of some of the components of the electron transport chain of Escherichia coli. The amounts of cytochromes reduced during aerobic steady-state co...

journal_title：Journal of bacteriology

authors： Downie JA,Cox GB

更新日期：1978-02-01 00:00:00

abstract：UNLABELLED:Mycobacterium tuberculosis possesses a thick and highly hydrophobic cell wall principally composed of a mycolyl-arabinogalactan-peptidoglycan complex, which is critical for survival and virulence. DprE1 is a well-characterized component of decaprenyl-phospho-ribose epimerase, which produces decaprenyl-phosph...

journal_title：Journal of bacteriology

authors： Kolly GS,Mukherjee R,Kilacsková E,Abriata LA,Raccaud M,Blaško J,Sala C,Dal Peraro M,Mikušová K,Cole ST

更新日期：2015-12-01 00:00:00