Abstract:
:Chromatin immunoprecipitation and microarray (ChIP-chip) analysis showed that the nitric oxide (NO)-sensitive repressor NsrR from Escherichia coli binds in vivo to the promoters of the tynA and feaB genes. These genes encode the first two enzymes of a pathway that is required for the catabolism of phenylethylamine (PEA) and its hydroxylated derivatives tyramine and dopamine. Deletion of nsrR caused small increases in the activities of the tynA and feaB promoters in cultures grown on PEA. Overexpression of nsrR severely retarded growth on PEA and caused a marked repression of the tynA and feaB promoters. Both the growth defect and the promoter repression were reversed in the presence of a source of NO. These results are consistent with NsrR mediating repression of the tynA and feaB genes by binding (in an NO-sensitive fashion) to the sites identified by ChIP-chip. E. coli was shown to use 3-nitrotyramine as a nitrogen source for growth, conditions which partially induce the tynA and feaB promoters. Mutation of tynA (but not feaB) prevented growth on 3-nitrotyramine. Growth yields, mutant phenotypes, and analyses of culture supernatants suggested that 3-nitrotyramine is oxidized to 4-hydroxy-3-nitrophenylacetate, with growth occurring at the expense of the amino group of 3-nitrotyramine. Accordingly, enzyme assays showed that 3-nitrotyramine and its oxidation product (4-hydroxy-3-nitrophenylacetaldehyde) could be oxidized by the enzymes encoded by tynA and feaB, respectively. The results suggest that an additional physiological role of the PEA catabolic pathway is to metabolize nitroaromatic compounds that may accumulate in cells exposed to NO.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Rankin LD,Bodenmiller DM,Partridge JD,Nishino SF,Spain JC,Spiro Sdoi
10.1128/JB.00508-08subject
Has Abstractpub_date
2008-09-01 00:00:00pages
6170-7issue
18eissn
0021-9193issn
1098-5530pii
JB.00508-08journal_volume
190pub_type
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doi:10.1128/JB.97.3.1328-1333.1969
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pub_type: 杂志文章
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doi:10.1128/jb.182.2.272-277.2000
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doi:10.1128/jb.177.22.6362-6370.1995
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doi:10.1128/JB.108.3.1366-1379.1971
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更新日期:1990-01-01 00:00:00
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更新日期:1982-06-01 00:00:00
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更新日期:1994-11-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/JB.127.1.595-609.1976
更新日期:1976-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1991-01-01 00:00:00