The unphosphorylated receiver domain of PhoB silences the activity of its output domain.

Abstract:

:PhoB is the response regulator of the Pho regulon. It is composed of two distinct domains, an N-terminal receiver domain and a C-terminal output domain that binds DNA and interacts with sigma(70) to activate transcription of the Pho regulon. Phosphorylation of the receiver domain is required for activation of the protein. The mechanism of activation by phosphorylation has not yet been determined. To better understand the function of the receiver domain in controlling the activity of the output domain, a direct comparison was made between unphosphorylated PhoB and its solitary DNA-binding domain (PhoB(DBD)) for DNA binding and transcriptional activation. Using fluorescence anisotropy, it was found that PhoB(DBD) bound to the pho box with an affinity seven times greater than that of unphosphorylated PhoB. It was also found that PhoB(DBD) was better able to activate transcription than the full-length, unmodified protein. We conclude that the unphosphorylated receiver domain of PhoB silences the activity of its output domain. These results suggest that upon phosphorylation of the receiver domain of PhoB, the inhibition placed upon the output domain is relieved by a conformational change that alters interactions between the unphosphorylated receiver domain and the output domain.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Ellison DW,McCleary WR

doi

10.1128/jb.182.23.6592-6597.2000

keywords:

subject

Has Abstract

pub_date

2000-12-01 00:00:00

pages

6592-7

issue

23

eissn

0021-9193

issn

1098-5530

journal_volume

182

pub_type

杂志文章
  • SULFATE REQUIREMENT FOR IRON OXIDATION BY THIOBACILLUS FERROOXIDANS.

    abstract::Lazaroff, Norman (British Columbia Research Council, Vancouver, B.C., Canada). Sulfate requirement for iron oxidation by Thiobacillus ferrooxidans. J. Bacteriol. 85:78-83. 1963.-The growth of Thiobacillus ferrooxidans is initially inhibited in media containing ferrous chloride in place of ferrous sulfate. This inhibit...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.85.1.78-83.1963

    authors: Lazaroff N

    更新日期:1963-01-01 00:00:00

  • Activation of the lac genes of Tn951 by insertion sequences from Pseudomonas cepacia.

    abstract::We have identified three transposable gene-activating elements from Pseudomonas cepacia on the basis of their abilities to increase expression of the lac genes of the broad-host-range plasmid pGC91.14 (pRP1::Tn951). When introduced into auxotrophic derivatives of P. cepacia 249 (ATCC 17616), this plasmid failed to con...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.4.1719-1724.1990

    authors: Wood MS,Lory C,Lessie TG

    更新日期:1990-04-01 00:00:00

  • Phase variation of Ag43 is independent of the oxidation state of OxyR.

    abstract::OxyR is a DNA binding protein that differentially regulates a cell's response to hydrogen peroxide-mediated oxidative stress. We previously reported that the reduced form of OxyR is sufficient for repression of transcription of agn43 from unmethylated template DNA, which is essential for deoxyadenosine methylase (Dam)...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.7.2203-2209.2003

    authors: Wallecha A,Correnti J,Munster V,van der Woude M

    更新日期:2003-04-01 00:00:00

  • Beta-glucoside (bgl) operon of Escherichia coli K-12: nucleotide sequence, genetic organization, and possible evolutionary relationship to regulatory components of two Bacillus subtilis genes.

    abstract::Wild-type Escherichia coli cells are unable to grow on beta-glucosides. Spontaneous mutants arise, however, which are able to utilize certain aromatic beta-glucosides such as salicin or arbutin as carbon sources, revealing the presence of a cryptic operon called bgl. Mutations activating the operon map within (or clos...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.6.2579-2590.1987

    authors: Schnetz K,Toloczyki C,Rak B

    更新日期:1987-06-01 00:00:00

  • Increased resistance to chloramphenicol in Rickettsia prowazekii with a note on failure to demonstrate genetic interaction among.

    abstract::Weiss, Emilio (Naval Medical Research Institute, Bethesda, Md.) and Harry R. Dressler. Increased resistance to chloramphenicol in Rickettsia prowazekii with a note on failure to demonstrate genetic interaction among strains. J. Bacteriol. 83:409-414. 1962.-A further effort to produce genetically labeled Rickettsia pro...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.2.409-414.1962

    authors: WEISS E,DRESSLER HR

    更新日期:1962-02-01 00:00:00

  • Evidence for distinct kynureninase and hydroxykynureninase activities in Neurospora crassa.

    abstract::Previous studies have indicated that a single enzyme, "kynureninase," catalyzes the reactions of l-kynurenine to anthranilate and l-3-hydroxykynurenine to 3-hydroxyanthranilate in Neurospora crassa and in other organisms. The present report describes separate enzymes which catalyze these reactions in N. crassa. The fi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.108.2.902-909.1971

    authors: Gaertner FH,Cole KW,Welch GR

    更新日期:1971-11-01 00:00:00

  • Determination of genome size, macrorestriction pattern polymorphism, and nonpigmentation-specific deletion in Yersinia pestis by pulsed-field gel electrophoresis.

    abstract::Of 16 restriction endonucleases known to hydrolyze rare 6- or 8-base recognition sequences that were tested, only SpeI, NotI, AscI, and SfiI generated fragments of chromosomal DNA from Yersinia pestis, the causative agent of bubonic plague, of sufficient length to permit physical analysis by pulsed-field gel electroph...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.7.2078-2086.1992

    authors: Lucier TS,Brubaker RR

    更新日期:1992-04-01 00:00:00

  • Complete genome sequence of multidrug-resistant Acinetobacter baumannii strain 1656-2, which forms sturdy biofilm.

    abstract::Acinetobacter baumannii is a Gram-negative bacterium causing nosocomial infections worldwide. To gain quick insight into the molecular basis of biofilm formation in A. baumannii, we determined the complete genome sequence of A. baumannii strain 1656-2, which forms sturdy biofilm and is resistant to multiple drugs. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06109-11

    authors: Park JY,Kim S,Kim SM,Cha SH,Lim SK,Kim J

    更新日期:2011-11-01 00:00:00

  • "Position effect" on dominance in the D-serine deaminase system of Escherichia coli K-12.

    abstract::A completely stable F32 merodiploid has been isolated, which has the dsd genotype dsdA(+)dsdCx3 /F -dsdA(+)dsdC(+). The stabilization apparently does not result from an alteration in the F, or from its integration in the chromosome, but rather from a chromosomal aberration. The stable strain can exist in two forms, wh...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.94.6.1989-1993.1967

    authors: McFall E

    更新日期:1967-12-01 00:00:00

  • Functions involved in bacteriophage P2-induced host cell lysis and identification of a new tail gene.

    abstract::Successful completion of the bacteriophage P2 lytic cycle requires phage-induced lysis of its Escherichia coli host, a process that is poorly understood. Genetic analysis of lysis-deficient mutants defined a single locus, gene K, which lies within the largest late transcription unit of P2 and maps between head gene L ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.16.4974-4984.1994

    authors: Ziermann R,Bartlett B,Calendar R,Christie GE

    更新日期:1994-08-01 00:00:00

  • The Hfq-dependent small noncoding RNA NrrF directly mediates Fur-dependent positive regulation of succinate dehydrogenase in Neisseria meningitidis.

    abstract::Previous microarray studies have suggested that an indirect mechanism of Fur regulation may be present in meningococcus at the posttranscriptional level through a small regulatory RNA (sRNA) system analogous to that of Escherichia coli and Pseudomonas aeruginosa. Recently, a Fur-regulated sRNA, NrrF, was identified th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00849-08

    authors: Metruccio MM,Fantappiè L,Serruto D,Muzzi A,Roncarati D,Donati C,Scarlato V,Delany I

    更新日期:2009-02-01 00:00:00

  • A defect in the acetyl coenzyme Aacetate pathway poisons recombinational repair-deficient mutants of Escherichia coli.

    abstract::Recombinational repair-dependent mutants identify ways to avoid chromosomal lesions. Starting with a recBC(Ts) strain of Escherichia coli, we looked for mutants unable to grow at 42 degrees C in conditions that inactivate the RecBCD(Ts) enzyme. We isolated insertions in ackA and pta, which comprise a two-gene operon r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.4.1266-1275.2005

    authors: Shi IY,Stansbury J,Kuzminov A

    更新日期:2005-02-01 00:00:00

  • The initiation codon affects ribosome binding and translational efficiency in Escherichia coli of cI mRNA with or without the 5' untranslated leader.

    abstract::Translational efficiency of an AUG, CUG, GUG, or UUG initiation codon was measured for the naturally leaderless cI mRNA from bacteriophage lambda. In a cI-lacZ translational fusion, only AUG supported a high level of expression; GUG supported a low level of expression, while UUG and CUG expression was barely above bac...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.4.1277-1283.2001

    authors: O'Donnell SM,Janssen GR

    更新日期:2001-02-01 00:00:00

  • Escherichia coli produces linoleic acid during late stationary phase.

    abstract::Escherichia coli produces linoleic acid in the late stationary phase. This was the case whether the cultures were grown aerobically or anaerobically on a supplemented glucose-salts medium. The linoleic acid was detected by thin-layer chromatography and was measured as the methyl ester by gas chromatography. The linole...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.17.5324-5328.1993

    authors: Rabinowitch HD,Sklan D,Chace DH,Stevens RD,Fridovich I

    更新日期:1993-09-01 00:00:00

  • tRNA Modification Profiles and Codon-Decoding Strategies in Methanocaldococcus jannaschii.

    abstract::tRNAs play a critical role in mRNA decoding, and posttranscriptional modifications within tRNAs drive decoding efficiency and accuracy. The types and positions of tRNA modifications in model bacteria have been extensively studied, and tRNA modifications in a few eukaryotic organisms have also been characterized and lo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00690-18

    authors: Yu N,Jora M,Solivio B,Thakur P,Acevedo-Rocha CG,Randau L,de Crécy-Lagard V,Addepalli B,Limbach PA

    更新日期:2019-04-09 00:00:00

  • Complete genomic sequence of the lytic bacteriophage phiYeO3-12 of Yersinia enterocolitica serotype O:3.

    abstract::phiYeO3-12 is a T3-related lytic bacteriophage of Yersinia enterocolitica serotype O:3. The nucleotide sequence of the 39,600-bp linear double-stranded DNA (dsDNA) genome was determined. The phage genome has direct terminal repeats of 232 bp, a GC content of 50.6%, and 54 putative genes, which are all transcribed from...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.6.1928-1937.2001

    authors: Pajunen MI,Kiljunen SJ,Söderholm ME,Skurnik M

    更新日期:2001-03-01 00:00:00

  • Escherichia coli-Salmonella typhimurium hybrid nusA genes: identification of a short motif required for action of the lambda N transcription antitermination protein.

    abstract::The Escherichia coli nusA gene, nusAEc, encodes an essential protein that influences transcription elongation. Derivatives of E. coli in which the Salmonella typhimurium nusA gene, nusASt, has replaced nusAEc are viable. Thus, NusASt can substitute for NusAEc in supporting essential bacterial activities. However, hybr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.5.1394-1404.1994

    authors: Craven MG,Granston AE,Schauer AT,Zheng C,Gray TA,Friedman DI

    更新日期:1994-03-01 00:00:00

  • Whole-genome sequence of Listeria welshimeri reveals common steps in genome reduction with Listeria innocua as compared to Listeria monocytogenes.

    abstract::We present the complete genome sequence of Listeria welshimeri, a nonpathogenic member of the genus Listeria. Listeria welshimeri harbors a circular chromosome of 2,814,130 bp with 2,780 open reading frames. Comparative genomic analysis of chromosomal regions between L. welshimeri, Listeria innocua, and Listeria monoc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00758-06

    authors: Hain T,Steinweg C,Kuenne CT,Billion A,Ghai R,Chatterjee SS,Domann E,Kärst U,Goesmann A,Bekel T,Bartels D,Kaiser O,Meyer F,Pühler A,Weisshaar B,Wehland J,Liang C,Dandekar T,Lampidis R,Kreft J,Goebel W,Chakraborty

    更新日期:2006-11-01 00:00:00

  • Role of plant root exudate and Sym plasmid-localized nodulation genes in the synthesis by Rhizobium leguminosarum of Tsr factor, which causes thick and short roots on common vetch.

    abstract::In a previous paper it was shown that cocultivation of Rhizobium leguminosarum with the plant Vicia sativa subsp. nigra on solid medium causes a changed mode of growth of the plant roots, resulting in thick and short roots (Tsr). The Sym plasmid present in the bacterium appeared to be essential for causing Tsr (A. A. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.165.2.517-522.1986

    authors: Van Brussel AA,Zaat SA,Cremers HC,Wijffelman CA,Pees E,Tak T,Lugtenberg BJ

    更新日期:1986-02-01 00:00:00

  • Major role of NAD-dependent lactate dehydrogenases in aerobic lactate utilization in Lactobacillus plantarum during early stationary phase.

    abstract::NAD-independent lactate dehydrogenases are commonly thought to be responsible for lactate utilization during the stationary phase of aerobic growth in Lactobacillus plantarum. To substantiate this view, we constructed single and double knockout mutants for the corresponding genes, loxD and loxL. Lactate-to-acetate con...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.19.6661-6666.2004

    authors: Goffin P,Lorquet F,Kleerebezem M,Hols P

    更新日期:2004-10-01 00:00:00

  • Different roles of DosS and DosT in the hypoxic adaptation of Mycobacteria.

    abstract::The DosS (DevS) and DosT histidine kinases form a two-component system together with the DosR (DevR) response regulator in Mycobacterium tuberculosis. DosS and DosT, which have high sequence similarity to each other over the length of their amino acid sequences, contain two GAF domains (GAF-A and GAF-B) in their N-ter...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00550-10

    authors: Kim MJ,Park KJ,Ko IJ,Kim YM,Oh JI

    更新日期:2010-10-01 00:00:00

  • METHICILLIN-INDUCED LYSOZYME-SENSITIVE FORMS OF STAPHYLOCOCCI.

    abstract::Aldrich, K. M. (The University of Kansas, Lawrence), and C. P. Sword. Methicillin-induced lysozyme-sensitive forms of staphylococci. J. Bacteriol. 87:690-695. 1964.-Staphylococcus aureus and S. epidermidis grown in the presence of sublethal amounts of methicillin were converted to enlarged spheres within 2 to 4 hr, as...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.3.690-695.1964

    authors: ALDRICH KM,SWORD CP

    更新日期:1964-03-01 00:00:00

  • The leucine-responsive regulatory protein, Lrp, modulates microcin J25 intrinsic resistance in Escherichia coli by regulating expression of the YojI microcin exporter.

    abstract::Many Escherichia coli K-12 strains display an intrinsic resistance to the peptide antibiotic microcin J25. In this study, we present results showing that the leucine-responsive regulatory protein, Lrp, is involved in this phenotype by acting as a positive regulator of YojI, a chromosomally encoded efflux pump which ex...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01074-08

    authors: Socías SB,Vincent PA,Salomón RA

    更新日期:2009-02-01 00:00:00

  • Physical and functional analyses of the syrA and syrB genes involved in syringomycin production by Pseudomonas syringae pv. syringae.

    abstract::The syrA and syrB genes involved in syringomycin production in Pseudomonas syringae pv. syringae B301D were identified from an EcoRI-pLAFR3 cosmid library and then physically and functionally analyzed in relation to plant pathogenicity. Homologous recombination of the genes required for syringomycin production from co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.12.5680-5688.1988

    authors: Xu GW,Gross DC

    更新日期:1988-12-01 00:00:00

  • A complex network regulates expression of eps and other virulence genes of Pseudomonas solanacearum.

    abstract::We have discovered an unusual and complex regulatory network used by the phytopathogen Pseudomonas solanacearum to control transcription of eps, which encodes for production of its primary virulence factor, the exopolysaccharide EPS I. The major modules of this network were shown to be three separate signal transducti...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.5.1259-1267.1995

    authors: Huang J,Carney BF,Denny TP,Weissinger AK,Schell MA

    更新日期:1995-03-01 00:00:00

  • Identification of a secreted cholesterol-dependent cytolysin (mitilysin) from Streptococcus mitis.

    abstract::We have detected a cholesterol-dependent cytolysin, which we have named mitilysin, in a small number of Streptococcus mitis isolates. We have sequenced the mitilysin gene from seven isolates of S. mitis. Comparisons with the pneumococcal pneumolysin gene show 15 amino acid substitutions. S. mitis appear to release mit...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01092-06

    authors: Jefferies J,Nieminen L,Kirkham LA,Johnston C,Smith A,Mitchell TJ

    更新日期:2007-01-01 00:00:00

  • Overproduction of Escherichia coli integration host factor, a protein with nonidentical subunits.

    abstract::Integration host factor (IHF) is a small, basic protein that is needed for efficient recombination of bacteriophage lambda, as well as for other host and viral functions. We have constructed strains in which the two subunits of IHF, encoded by the himA and hip genes of Escherichia coli, are expressed under the control...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.9.4124-4127.1987

    authors: Nash HA,Robertson CA,Flamm E,Weisberg RA,Miller HI

    更新日期:1987-09-01 00:00:00

  • Modulation of anaerobic energy metabolism of Bacillus subtilis by arfM (ywiD).

    abstract::Bacillus subtilis grows under anaerobic conditions utilizing nitrate ammonification and various fermentative processes. The two-component regulatory system ResDE and the redox regulator Fnr are the currently known parts of the regulatory system for anaerobic adaptation. Mutation of the open reading frame ywiD located ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.23.6815-6821.2001

    authors: Marino M,Ramos HC,Hoffmann T,Glaser P,Jahn D

    更新日期:2001-12-01 00:00:00

  • cobB function is required for catabolism of propionate in Salmonella typhimurium LT2: evidence for existence of a substitute function for CobB within the 1,2-propanediol utilization (pdu) operon.

    abstract::The cobB function of Salmonella typhimurium LT2 was defined in vivo as an alternative activity for the nicotinic acid mononucleotide:5,6-dimethylbenzimidazole phosphoribosyltransferase enzyme (CobT), which is involved in the assembly of the nucleotide loop of cobalamin in this bacterium (J. R. Trzebiatowski, G. A. O'T...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.23.7016-7019.1996

    authors: Tsang AW,Escalante-Semerena JC

    更新日期:1996-12-01 00:00:00

  • Effect of pH on the proportions of polar lipids, in chemostat cultures of Bacillus subtilis.

    abstract::Significant changes in the relative proportions of the individual polar lipids of two strains of Bacillus subtilis were observed when the pH of their chemostat cultures was varied. In phosphate- and magnesium-limited cultures of B. subtilis var. niger NCIB 8058. lysylphosphatidylglycerol was present in higher proporti...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.120.3.999-1003.1974

    authors: Minnikin DE,Abdolrahimzadeh H

    更新日期:1974-12-01 00:00:00