Mismatch correction in pneumococcal transformation: donor length and hex-dependent marker efficiency.

abstract：:A number of polyacrylamide gel systems and solubilization procedures were studied to define the number and nature of "major" polypeptide bands in the outer membrane of Pseudomonas aeruginosa. It was shown that five of the eight major outer membrane proteins were "heat modifiable" in that their mobility on sodium dodec...

journal_title：Journal of bacteriology

authors： Hancock RE,Carey AM

更新日期：1979-12-01 00:00:00

abstract：:A deoxyribonucleic acid-directed in vitro system for the synthesis of Escherichia coli carbamoylphosphate synthase has been developed, and its properties have been studied. The system uses the deoxyribonucleic acid of a lambda phage carrying the car genes (lambdadcarAB) as template and mediates the synthesis of both s...

journal_title：Journal of bacteriology

authors： Lissens W,Cunin R,Kelker N,Glansdorff N,Piérard A

更新日期：1980-01-01 00:00:00

abstract：:Korormicin is an antibiotic produced by some pseudoalteromonads which selectively kills Gram-negative bacteria that express the Na+-pumping NADH:quinone oxidoreductase (Na+-NQR.) We show that although korormicin is an inhibitor of Na+-NQR, the antibiotic action is not a direct result of inhibiting enzyme activity. Ins...

journal_title：Journal of bacteriology

authors： Maynard A,Butler NL,Ito T,da Silva AJ,Murai M,Chen T,Koffas MAG,Miyoshi H,Barquera B

更新日期：2019-05-08 00:00:00

abstract：:Supercoiled double-stranded DNA molecules (plasmids) were isolated from plants infected with three laboratory strains of western aster yellows mycoplasma-like organism (AY-MLO) by using cesium chloride-ethidium bromide density gradients. Southern blot analysis, using plasmids from the severe strain of AY-MLO (SAY-MLO)...

journal_title：Journal of bacteriology

authors： Kuske CR,Kirkpatrick BC

更新日期：1990-03-01 00:00:00

abstract：:We have found, using a newly developed genetic method, a protein (named Cnu, for oriC-binding nucleoid-associated) that binds to a specific 26-base-pair sequence (named cnb) in the origin of replication of Escherichia coli, oriC. Cnu is composed of 71 amino acids (8.4 kDa) and shows extensive amino acid identity to a ...

journal_title：Journal of bacteriology

authors： Kim MS,Bae SH,Yun SH,Lee HJ,Ji SC,Lee JH,Srivastava P,Lee SH,Chae H,Lee Y,Choi BS,Chattoraj DK,Lim HM

更新日期：2005-10-01 00:00:00

abstract：:Despite the fact that phosphoenolpyruvate carboxylase (PEPC) activity has been measured and in some cases even purified from some Archaea, the gene responsible for this activity has not been elucidated. Using sensitive sequence comparison methods, we detected a highly conserved, uncharacterized archaeal gene family th...

journal_title：Journal of bacteriology

authors： Ettema TJ,Makarova KS,Jellema GL,Gierman HJ,Koonin EV,Huynen MA,de Vos WM,van der Oost J

更新日期：2004-11-01 00:00:00

abstract：:Bacterial chemotaxis is based on modulation of the probability to switch the direction of flagellar rotation. Responses to many stimuli are transduced by a two-component system via reversible phosphorylation of CheY, a small cytoplasmic protein that directly interacts with the switch complex at the flagellar motor. We...

journal_title：Journal of bacteriology

authors： Montrone M,Oesterhelt D,Marwan W

更新日期：1996-12-01 00:00:00

abstract：:Clostridium difficile, a proteolytic Gram-positive anaerobe, has emerged as a significant nosocomial pathogen. Stickland fermentation reactions are thought to be important for growth of C. difficile and appear to influence toxin production. In Stickland reactions, pairs of amino acids donate and accept electrons, gene...

journal_title：Journal of bacteriology

authors： Bouillaut L,Self WT,Sonenshein AL

更新日期：2013-02-01 00:00:00

abstract：:A mutation in a new gene, molR, prevented the synthesis in Escherichia coli of molybdoenzymes, including the two formate dehydrogenase isoenzymes, nitrate reductase and trimethylamine-N-oxide reductase. This phenotype was suppressed by supplementing the media with molybdate. Thus, the molR mutant was phenotypically si...

journal_title：Journal of bacteriology

authors： Lee JH,Wendt JC,Shanmugam KT

更新日期：1990-04-01 00:00:00

abstract：:In Escherichia coli, rotation of the flagellar motor has been shown to depend upon electrostatic interactions between charged residues of the stator protein MotA and the rotor protein FliG. These charged residues are conserved in the Na+-driven polar flagellum of Vibrio alginolyticus, but mutational studies in V. algi...

journal_title：Journal of bacteriology

authors： Yakushi T,Yang J,Fukuoka H,Homma M,Blair DF

更新日期：2006-02-01 00:00:00

abstract：:During depletion of 4.5S RNA, cells of Escherichia coli displayed a heat shock response that was simultaneous with the first detectable effect on ribosome function and before major effects on cell growth. Either 4.5S RNA is involved directly in regulating the heat shock response, or this particular impairment of prote...

journal_title：Journal of bacteriology

authors： Bourgaize DB,Phillips TA,VanBogelen RA,Jones PG,Neidhardt FC,Fournier MJ

更新日期：1990-02-01 00:00:00

abstract：:Orthologs of RecG and RuvABC are highly conserved among prokaryotes; in Escherichia coli, they participate in independent pathways that branch migrate Holliday junctions during recombinational DNA repair. RecG also has been shown to directly convert stalled replication forks into Holliday junctions. The bacterium Heli...

journal_title：Journal of bacteriology

authors： Kang J,Blaser MJ

更新日期：2008-06-01 00:00:00

abstract：:The activity of the toluene-responsive sigma(54) Pu promoter of the pWW0 TOL plasmid of Pseudomonas putida is down-regulated in vivo during exponential growth in rich medium and also by the presence of glucose in the culture. Although the Pu promoter already performs poorly during log growth in minimal medium when ame...

journal_title：Journal of bacteriology

authors： Cases I,de Lorenzo V

更新日期：2000-02-01 00:00:00

abstract：:The location and extent of the homology between plasmids ColE1 and p15A were determined by analysis of heteroduplexes formed between them as well as with a related plasmid, pBR322, and by hybridization of radioactive deoxyribonucleic acids to restriction fragments of p15A and ColE1. The homology between the plasmids c...

journal_title：Journal of bacteriology

authors： Bird RE

更新日期：1981-03-01 00:00:00

abstract：:The endophytic diazotroph Gluconacetobacter diazotrophicus secretes a constitutively expressed levansucrase (LsdA, EC 2.4.1.10) to utilize plant sucrose. LsdA, unlike other extracellular levansucrases from gram-negative bacteria, is transported to the periplasm by a signal-peptide-dependent pathway. We identified an u...

journal_title：Journal of bacteriology

authors： Arrieta JG,Sotolongo M,Menéndez C,Alfonso D,Trujillo LE,Soto M,Ramírez R,Hernández L

更新日期：2004-08-01 00:00:00

abstract：:Protein K is an outer membrane protein found in pathogenic encapsulated strains of Escherichia coli. We present evidence here that protein K is structurally and functionally related to the E. coli K-12 porin proteins (OmpF, OmpC, and PhoE). Protein K was found to cross-react with antibody to OmpF protein and to share ...

journal_title：Journal of bacteriology

authors： Sutcliffe J,Blumenthal R,Walter A,Foulds J

更新日期：1983-11-01 00:00:00

abstract：:In Pseudomonas putida DOT-T1E multidrug efflux pumps of the resistance-nodulation-division family make a major contribution to solvent resistance. Two pumps have been identified: TtgABC, expressed constitutively, and TtgDEF, induced by aromatic hydrocarbons. A double mutant lacking both efflux pumps was able to surviv...

journal_title：Journal of bacteriology

authors： Rojas A,Duque E,Mosqueda G,Golden G,Hurtado A,Ramos JL,Segura A

更新日期：2001-07-01 00:00:00

abstract：:The Brucella abortus virB locus contains 12 open reading frames, termed virB1 through virB12, which encode a type IV secretion system. Polar mutations in the virB locus markedly reduce the ability of B. abortus to survive in cultured macrophages or to persist in organs of mice. While a nonpolar deletion of the virB2 g...

journal_title：Journal of bacteriology

authors： den Hartigh AB,Rolán HG,de Jong MF,Tsolis RM

更新日期：2008-07-01 00:00:00

abstract：:Open reading frame orf192, which is located immediately upstream of the aerobic repressor gene crtJ, was genetically and biochemically demonstrated to code for a second aerobic repressor (AerR) of photosynthesis gene expression in Rhodobacter capsulatus. Promoter-mapping studies indicate that crtJ has its own promoter...

journal_title：Journal of bacteriology

authors： Dong C,Elsen S,Swem LR,Bauer CE

更新日期：2002-05-01 00:00:00

abstract：:The spirochete Borrelia hermsii, a relapsing fever agent, evades the host's immune response through multiphasic antigenic variation. Antigen switching results from sequential expression of genes for serotype-specific outer membrane proteins known as variable major proteins (Vmp's); of the 25 serotypes that have been i...

journal_title：Journal of bacteriology

authors： Kitten T,Barrera AV,Barbour AG

更新日期：1993-05-01 00:00:00

abstract：:Yersinia pestis, which causes bubonic and pneumonic plague, forms pigmented red colonies on Congo red (CR) dye agar. The hmsHFRS genes required for CR binding (Crb(+)) are genetically linked to virulence-associated genes encoding a siderophore uptake system. These genes are contained in a 102-kb chromosomal pgm locus ...

journal_title：Journal of bacteriology

authors： Hare JM,McDonough KA

更新日期：1999-08-01 00:00:00

abstract：:We have used a plasmid-based transcriptional reporter system to examine the transcriptional effects of 33 single point mutations in the box A region (TATA-like sequence) of the Haloferax volcanii tRNA(Lys) promoter. The most pronounced effects on transcriptional efficiency were found when the nucleotides corresponding...

journal_title：Journal of bacteriology

authors： Palmer JR,Daniels CJ

更新日期：1995-04-01 00:00:00

abstract：:Treatment of Escherichia coli with p-hydroxybenzoic acid (pHBA) resulted in upregulation of yhcP, encoding a protein of the putative efflux protein family. Also upregulated were the adjacent genes yhcQ, encoding a protein of the membrane fusion protein family, and yhcR, encoding a small protein without a known or sugg...

journal_title：Journal of bacteriology

authors： Van Dyk TK,Templeton LJ,Cantera KA,Sharpe PL,Sariaslani FS

更新日期：2004-11-01 00:00:00

abstract：:Changes in the cell wall of a species of Flavobacterium can be quantitated by measuring vancomycin adsorption. Treatment of the cells with ethylenediaminetetraacetic acid or tris(hydroxymethyl)aminomethane (or both) increased adsorption of the antibiotic. ...

journal_title：Journal of bacteriology

authors： Haslam DF,Best GK,Durham NN

更新日期：1970-08-01 00:00:00

abstract：:Lockart, Royce Z., Jr. (The University of Texas, Austin) and Barbara Horn. Interaction of an interferon with L cells. J. Bacteriol. 85:996-1002. 1963.-Data were presented on the effect of time of exposure and concentration of an interferon in provoking viral inhibition in L cells. Populations of L cells which made red...

journal_title：Journal of bacteriology

authors： LOCKART RZ Jr,HORN B

更新日期：1963-05-01 00:00:00

abstract：:Several characteristics of Mycobacterium tuberculosis (e.g., conserved genome and low growth rate) have severely restricted the study of the microorganism. The discovery of IS6110 raised hopes of overcoming these obstacles. However, our knowledge of this IS element is relatively limited; even its two basic characteris...

journal_title：Journal of bacteriology

authors： Fang Z,Kenna DT,Doig C,Smittipat DN,Palittapongarnpim P,Watt B,Forbes KJ

更新日期：2001-09-01 00:00:00

abstract：:Valentine, R. C. (University of Illinois, Urbana), H. Drucker, and R. S. Wolfe. Glyoxylate fermentation by Streptococcus allantoicus. J. Bacteriol. 87:241-246. 1964.-Extracts of Streptococcus allantoicus were found to degrade glyoxylate, yielding tartronic semialdehyde and CO(2). Tartronic semialdehyde was prepared ch...

journal_title：Journal of bacteriology

authors： VALENTINE RC,DRUCKER H,WOLFE RS

更新日期：1964-02-01 00:00:00

abstract：:The structural gene for the F sex factor outer membrane surface exclusion protein ( traT ) was cloned onto a high-copy-number plasmid where it is expressed from the phage lambda promoter pL. Conditional control over expression was provided by a temperature-sensitive lambda cI repressor. Induction of pL produced large ...

journal_title：Journal of bacteriology

authors： Minkley EG Jr

更新日期：1984-05-01 00:00:00

abstract：:Bacillus brevis HPD31 contains a surface (S)-layer protein, termed the HWP, which forms a hexagonal array in the cell wall. The 5' region of the HWP gene was isolated from a DNA library constructed in bacteriophage vector EMBL3 from a partial BamHI digest of the chromosomal DNA. The 3' region contained in a 2.7-kiloba...

journal_title：Journal of bacteriology

authors： Ebisu S,Tsuboi A,Takagi H,Naruse Y,Yamagata H,Tsukagoshi N,Udaka S

更新日期：1990-03-01 00:00:00

abstract：:The high-potential 4Fe-4S* center ofAzotobacter vinelandii ferredoxin I has been titrated potentiometrically by a reductive procedure. The absorbance decrease at 510 nm accompanying the reduction of the high-potential center titrated with an Em of 320 mV (n = 1). The low-potential 4Fe-4S* center was titrated by using ...

journal_title：Journal of bacteriology

authors： Yoch DC,Carithers RP

更新日期：1978-11-01 00:00:00