Escherichia coli outer membrane protein K is a porin.

Abstract:

:Protein K is an outer membrane protein found in pathogenic encapsulated strains of Escherichia coli. We present evidence here that protein K is structurally and functionally related to the E. coli K-12 porin proteins (OmpF, OmpC, and PhoE). Protein K was found to cross-react with antibody to OmpF protein and to share 8 out of 17 peptides in common with the OmpF protein. Strains that are OmpC porin- and OmpF porin- and contain protein K as their major outer membrane protein have increased rates of uptake of nutrients and a faster growth rate relative to the parental porin- strain. The protein K-containing strains are at least 1,000-fold more sensitive to colicins E2 and E3 than is the porin -deficient strain. These data suggest that protein K is a functional porin in E. coli. The porin function of protein K was also demonstrated in vitro, using black lipid membranes. Protein K increased the conductance in these membranes in discrete, uniform steps characteristic of channels with a size of about 2 nS.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Sutcliffe J,Blumenthal R,Walter A,Foulds J

doi

10.1128/JB.156.2.867-872.1983

subject

Has Abstract

pub_date

1983-11-01 00:00:00

pages

867-72

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

156

pub_type

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