Facilitated diffusion of monosaccharides in Saccharomyces cerevisiae: experimental investigation of kinetic parameters without the assumptions of symmetry.

abstract：:The lactose-H+ symport protein (LacS) of Streptococcus thermophilus has a carboxyl-terminal regulatory domain (IIALacS) that is homologous to a family of proteins and protein domains of the phosphoenolpyruvate:carbohydrate phosphotransferase system (PTS) in various organisms, of which IIAGlc of Escherichia coli is the...

journal_title：Journal of bacteriology

authors： Gunnewijk MG,Postma PW,Poolman B

更新日期：1999-01-01 00:00:00

abstract：:Glycosylation is a common modulation of protein function in eukaryotes and is biologically important. However, in bacteria protein glycosylation is rare, and relatively few bacterial glycoproteins are known. In Escherichia coli only two glycoproteins have been described to date. Here we introduce a novel member to thi...

journal_title：Journal of bacteriology

authors： Sherlock O,Dobrindt U,Jensen JB,Munk Vejborg R,Klemm P

更新日期：2006-03-01 00:00:00

abstract：:A series of mutations of the P1 plasmid prophage that lead to increased copy number was isolated and analyzed. The copy number of the mutants was elevated at least five- to eightfold relative to wild-type P1, as determined by single-cell resistance to antibiotics, activity of enzymes, content of superhelical DNA, and ...

journal_title：Journal of bacteriology

authors： Scott JR,Kropf MM,Padolsky L,Goodspeed JK,Davis R,Vapnek D

更新日期：1982-06-01 00:00:00

abstract：:The terminal step of triacylglycerol (TAG) formation in the yeast Saccharomyces cerevisiae is catalyzed by the enzyme acyl-CoA:diacylglycerol acyltransferase (DAGAT). In this study we demonstrate that the gene product of YOR245c, Dga1p, catalyzes a major yeast DAGAT activity which is localized to lipid particles. Enzy...

journal_title：Journal of bacteriology

authors： Sorger D,Daum G

更新日期：2002-01-01 00:00:00

abstract：:Mutants defective in uptake or utilization of a given metabolite can readily be obtained from facultative auxotrophs (for that metabolite) by penicillin enrichment under nonpermissive conditions in the presence of a low level of the required metabolite. ...

journal_title：Journal of bacteriology

authors： Berg CM,Rossi JJ,Coleman JV,Conlon M

更新日期：1975-03-01 00:00:00

abstract：:Streptococcus pseudopneumoniae is a member of the viridans group streptococci (VGS) whose pathogenic significance is unclear. We announce the complete genome sequence of S. pseudopneumoniae IS7493. The genome sequence will assist in the characterization of this new organism and facilitate the development of accurate d...

journal_title：Journal of bacteriology

authors： Shahinas D,Tamber GS,Arya G,Wong A,Lau R,Jamieson F,Ma JH,Alexander DC,Low DE,Pillai DR

更新日期：2011-11-01 00:00:00

abstract：UNLABELLED:Streptococcus pneumoniae is able to integrate exogenous DNA into its genome by natural genetic transformation. Transient accumulation of high levels of the only S. pneumoniae alternative σ factor is insufficient for development of full competence without expression of a second competence-specific protein, Co...

journal_title：Journal of bacteriology

authors： Tovpeko Y,Bai J,Morrison DA

更新日期：2016-08-11 00:00:00

abstract：:The organization and transcriptional control of chromosomal cat genes (required for dissimilation of catechol by the beta-ketoadipate pathway) in the Pseudomonas putida biotype strain (ATCC 12633) are reported. Nucleotide sequence reveals that catR is separated by 135 bp from the divergently transcribed catBC,A; catC ...

journal_title：Journal of bacteriology

authors： Houghton JE,Brown TM,Appel AJ,Hughes EJ,Ornston LN

更新日期：1995-01-01 00:00:00

abstract：:Site-directed mutagenesis has previously been used to construct Escherichia coli dnaK mutants encoding proteins that are altered at the site of in vitro phosphorylation (J. S. McCarty and G. C. Walker, Proc. Natl. Acad. Sci. USA 88:9513-9517, 1991). These mutants are unable to autophosphorylate and are severely defect...

journal_title：Journal of bacteriology

authors： McCarty JS,Walker GC

更新日期：1994-02-01 00:00:00

abstract：:Vibrio vulnificus is an estuarine bacterium and potent opportunistic human pathogen. It enters the food chain by asymptomatically colonizing a variety of marine organisms, most notably oysters. Expression of the brp-encoded extracellular polysaccharide, which enhances cell-surface adherence, is regulated by cyclic di-...

journal_title：Journal of bacteriology

authors： Chodur DM,Guo L,Pu M,Bruger E,Fernandez N,Waters C,Rowe-Magnus DA

更新日期：2017-09-05 00:00:00

abstract：:Sixteen species of enterobacteria have been screened for mutagenic DNA repair activity. In Escherichia coli, mutagenic DNA repair is encoded by the umuDC operon. Synthesis of UmuD and UmuC proteins is induced as part of the SOS response to DNA damage, and after induction, the UmuD protein undergoes an autocatalytic cl...

journal_title：Journal of bacteriology

authors： Sedgwick SG,Ho C,Woodgate R

更新日期：1991-09-01 00:00:00

abstract：:lac operon fusions to DNA damage-inducible (din) loci were generated in Salmonella typhimurium LT2. Many of these din fusions were efficiently repressed by cloned Escherichia coli LexA, while others were not; all required RecA for induction. Several din fusions exhibited strong inducibility and will be useful in devel...

journal_title：Journal of bacteriology

authors： Smith CM,Arany Z,Orrego C,Eisenstadt E

更新日期：1991-06-01 00:00:00

abstract：:AppppA and the DnaK protein have both been hypothesized to function in regulating the heat shock response of Escherichia coli. The proposals are that AppppA serves as a signal (alarmone) to turn on the heat shock response, whereas the DnaK protein is necessary to turn off the heat shock response. A simple model would ...

journal_title：Journal of bacteriology

authors： Bochner BR,Zylicz M,Georgopoulos C

更新日期：1986-11-01 00:00:00

abstract：:A specific gamma-aminobutyrate (GABA) transport system in Escherichia coli K-12 cells with a K(m) of 12 muM and a V(max) of 278 nmol/ml of intracellular water per min is described. Membrane vesicles contained d-lactate-dependent activity of the system. Mutants defective in GABA transport were isolated; they lost the a...

journal_title：Journal of bacteriology

authors： Kahane S,Levitz R,Halpern YS

更新日期：1978-08-01 00:00:00

abstract：:A class II restriction endonuclease which recognizes the same nucleotide sequence as EndoR.HaeIII has been found in four of seven isolates of Neisseria gonorrhoeae tested. ...

journal_title：Journal of bacteriology

authors： Clanton DJ,Woodward JM,Miller RV

更新日期：1978-07-01 00:00:00

abstract：:Early log-phase cells of Mycoplasma gallisepticum A5969 were synchronized by holding in Eagle minimal essential medium (MEM) for 2 h. When transferred out of MEM into tryptose medium, the cells exhibited synchronous growth. Deoxyribonucleic acid (DNA) synthesis proceeded continuously during this growth but stopped dur...

journal_title：Journal of bacteriology

authors： Quinlan DC,Maniloff J

更新日期：1973-07-01 00:00:00

abstract：:Jones, Lois M. (University of Wisconsin, Madison), C. R. McDuff, and J. B. Wilson. Phenotypic alterations in the colonial morphology of Brucella abortus due to a bacteriophage carrier state. J. Bacteriol. 83:860-866. 1962.-In the course of examining a number of Brucella cultures with a brucellaphage, it was observed t...

journal_title：Journal of bacteriology

authors： JONES LM,McDUFF CR,WILSON JB

更新日期：1962-04-01 00:00:00

abstract：:Despite their remarkable genetic homology, members of the Mycobacterium tuberculosis complex express very different phenotypes, most notably in their spectra of clinical presentation. For example, M. tuberculosis is regarded as pathogenic to humans, whereas members having deleted RD1, such as Mycobacterium microti and...

journal_title：Journal of bacteriology

authors： Mostowy S,Cousins D,Behr MA

更新日期：2004-01-01 00:00:00

abstract：:A method for the transformation of Bacteroides fragilis with plasmid DNA was developed by using the clindamycin resistance plasmid pBFTM10 as the source of transforming DNA. The method was technically simple to perform and resulted in an average of 4.2 X 10(3) transformants per microgram of pBFTM10 added. A method for...

journal_title：Journal of bacteriology

authors： Smith CJ

更新日期：1985-10-01 00:00:00

abstract：:The sequence (2,700 bp) between the aldH and pspF genes of Escherichia coli was determined. The pspF gene encodes a sigma54 transcriptional activator of the phage shock protein (psp) operon (pspA to pspE). Downstream of the pspF transcribed region are two open reading frames (ORFs), ordL and goaG, convergently oriente...

journal_title：Journal of bacteriology

authors： Jovanovic G,Model P

更新日期：1997-05-01 00:00:00

abstract：:The RNA polymerase (RNAP) binding protein A (RbpA) contributes to the formation of stable RNAP-promoter open complexes (RPo) and is essential for viability in mycobacteria. Four domains have been identified in the RbpA protein, i.e., an N-terminal tail (NTT) that interacts with RNAP β' and σ subunits, a core domain (C...

journal_title：Journal of bacteriology

authors： Prusa J,Jensen D,Santiago-Collazo G,Pope SS,Garner AL,Miller JJ,Ruiz Manzano A,Galburt EA,Stallings CL

更新日期：2018-06-11 00:00:00

abstract：:We report here direct evidence that mutations in the par locus affect protein-DNA interactions in vivo at the replication origin of plasmid pSC101. Concomitant with par-mediated plasmid stabilization, two sites in the origin region show an altered methylation pattern as detected by in vivo footprinting with dimethyl s...

journal_title：Journal of bacteriology

authors： Ingmer H,Cohen SN

更新日期：1993-09-01 00:00:00

abstract：:pBL2 was identified genetically but not physically in Streptomyces lividans after its mating with S. bambergiensis. During conjugation, pBL2 was transferred at high frequency to S. lividans and S. coelicolor. pBL2.1 DNA isolated from S. coelicolor exconjugants as a circular plasmid was shown to derive from the genome ...

journal_title：Journal of bacteriology

authors： Zotchev SB,Schrempf H,Hutchinson CR

更新日期：1995-08-01 00:00:00

abstract：:The Rhizobium leguminosarum biovar viciae genome contains several genes predicted to determine surface polysaccharides. Mutants predicted to affect the initial steps of polysaccharide synthesis were identified and characterized. In addition to the known cellulose (cel) and acidic exopolysaccharide (EPS) (pss) genes, w...

journal_title：Journal of bacteriology

authors： Williams A,Wilkinson A,Krehenbrink M,Russo DM,Zorreguieta A,Downie JA

更新日期：2008-07-01 00:00:00

abstract：:The rpsD gene, encoding ribosomal protein S4, was isolated from Bacillus subtilis by hybridization with oligonucleotide probes derived from the S4 amino-terminal protein sequence. Sequence analysis of the cloned DNA indicated that rpsD is likely to be monocistronic, in contrast to Escherichia coli rpsD, which is locat...

journal_title：Journal of bacteriology

authors： Grundy FJ,Henkin TM

更新日期：1990-11-01 00:00:00

abstract：:mRNA levels from the bop (bacterio-opsin), brp (bacterio-opsin-related protein), and bat (bacterio-opsin activator) genes in wild-type Halobacterium halobium and two bacteriorhodopsin-overproducing mutants (ET1001 and II-7) were quantitated under conditions in which oxygen levels were steadily depleted and then cultur...

journal_title：Journal of bacteriology

authors： Shand RF,Betlach MC

更新日期：1994-03-01 00:00:00

abstract：:A map location of the gluconate-6-phosphate dehydrogenase (gnd) marker was estimated in Escherichia coli C at approximately 46 min by P1 transduction. The gnd locus appears to lie between the co-transducible histidine and prophage P2 location I markers. ...

journal_title：Journal of bacteriology

authors： Robbins RS,Kelly B

更新日期：1973-11-01 00:00:00

abstract：:Acidic hot springs are colonized by a diversity of hyperthermophilic organisms requiring extremes of temperature and pH for growth. To clarify how carbohydrates are consumed in such locations, the structural gene (malA) encoding the major soluble alpha-glucosidase (maltase) and flanking sequences from Sulfolobus solfa...

journal_title：Journal of bacteriology

authors： Rolfsmeier M,Haseltine C,Bini E,Clark A,Blum P

更新日期：1998-03-01 00:00:00

abstract：:Two isoenzymes of malate dehydrogenase (MDH) were demonstrated in plasmodia of Physarum polycephalum by polyacrylamide-gel electrophoresis. The more "cathodal" form was uniquely associated with mitochondria (M-MDH) and the other form was found in the soluble cytoplasm (S-MDH). The isoenzymes were separated by acetone ...

journal_title：Journal of bacteriology

authors： Teague WM,Henney HR Jr

更新日期：1973-11-01 00:00:00

abstract：:Bulk membrane fragments were prepared from cells of Bacillus cereus ATCC 4342 harvested at different stages of growth and sporulation and examined for enzymes involved in electron transport functions. The presence of succinate: DCPIP oxidoreductase (EC 1.3.99.1), succinate: cytochrome c oxidoreductase (EC 1.3.2.1), NA...

journal_title：Journal of bacteriology

authors： Lang DR,Felix J,Lundgren DG

更新日期：1972-06-01 00:00:00