The pSC101 par locus alters protein-DNA interactions in vivo at the plasmid replication origin.

Abstract:

:We report here direct evidence that mutations in the par locus affect protein-DNA interactions in vivo at the replication origin of plasmid pSC101. Concomitant with par-mediated plasmid stabilization, two sites in the origin region show an altered methylation pattern as detected by in vivo footprinting with dimethyl sulfate. One site is located near an integration host factor-binding sequence adjacent to the first of three direct repeats known to be involved in the initiation of pSC101 replication; the second site is within the third direct repeat.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Ingmer H,Cohen SN

doi

10.1128/jb.175.18.6046-6048.1993

subject

Has Abstract

pub_date

1993-09-01 00:00:00

pages

6046-8

issue

18

eissn

0021-9193

issn

1098-5530

journal_volume

175

pub_type

杂志文章
  • A region in the Bacillus subtilis transcription factor Spo0A that is important for spoIIG promoter activation.

    abstract::Spo0A is a DNA binding protein in Bacillus subtilis required for the activation of spoIIG and other promoters at the onset of endospore formation. Activation of some of these promoters may involve interaction of Spo0A and the sigmaA subunit of RNA polymerase. Previous studies identified two single-amino-acid substitut...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.14.3578-3583.1998

    authors: Buckner CM,Schyns G,Moran CP Jr

    更新日期:1998-07-01 00:00:00

  • Identification of a DtxR-regulated operon that is essential for siderophore-dependent iron uptake in Corynebacterium diphtheriae.

    abstract::The diphtheria toxin repressor (DtxR) uses Fe(2+) as a corepressor and inhibits transcription from iron-regulated promoters (IRPs) in Corynebacterium diphtheriae. A new IRP, designated IRP6, was cloned from C. diphtheriae by a SELEX-like procedure. DtxR bound to IRP6 in vitro only in the presence of appropriate divale...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.17.4846-4856.2002

    authors: Qian Y,Lee JH,Holmes RK

    更新日期:2002-09-01 00:00:00

  • Inhibition of thiamine transport by chloroethylthiamine in Escherichia coli.

    abstract::Chloroethylthiamine was found to inhibit an entrapment of thiamine as thiamine monophosphate by blocking thiamine monophosphokinase in the cytoplasm after thiamine was taken up by the cells of Escherichia coli. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.112.3.1438-1440.1972

    authors: Iwashima A,Nose Y

    更新日期:1972-12-01 00:00:00

  • Effect of nitrate on the autotrophic metabolism of the acetogens Clostridium thermoautotrophicum and Clostridium thermoaceticum.

    abstract::Although nitrate stimulated the capacity of Clostridium thermoautotrophicum and Clostridium thermoaceticum to oxidize (utilize) substrates under heterotrophic conditions, it inhibited autotrophic H2-CO2-dependent growth. Under basal medium conditions, nitrate was also inhibitory to the use of one-carbon substrates (i....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.15.4597-4603.1996

    authors: Fröstl JM,Seifritz C,Drake HL

    更新日期:1996-08-01 00:00:00

  • Analysis of plasmid deletional instability in Bacillus subtilis.

    abstract::Using a model system, we have studied deletion formation in Bacillus subtilis. When the staphylococcal plasmids pSA2100 (7.1 kilobases) and pUB110 (4.5 kilobases) were ligated to one another at their unique XbaI sites and transformed into either rec+ or recE4 strains of B. subtilis, an intramolecular recombination eve...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.162.3.1014-1023.1985

    authors: Hahn J,Dubnau D

    更新日期:1985-06-01 00:00:00

  • CfaD-dependent expression of a novel extracytoplasmic protein from enterotoxigenic Escherichia coli.

    abstract::H10407 is a strain of enterotoxigenic Escherichia coli (ETEC) that utilizes CFA/I pili to adhere to surfaces of the small intestine, where it elaborates toxins that cause profuse watery diarrhea in humans. Expression of the CFA/I pilus is positively regulated at the level of transcription by CfaD, a member of the AraC...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00131-07

    authors: Pilonieta MC,Bodero MD,Munson GP

    更新日期:2007-07-01 00:00:00

  • In vivo DNA binding of bacteriophage GA-1 protein p6.

    abstract::Bacteriophage GA-1 infects Bacillus sp. strain G1R and has a linear double-stranded DNA genome with a terminal protein covalently linked to its 5' ends. GA-1 protein p6 is very abundant in infected cells and binds DNA with no sequence specificity. We show here that it binds in vivo to the whole viral genome, as detect...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01047-07

    authors: Alcorlo M,Salas M,Hermoso JM

    更新日期:2007-11-01 00:00:00

  • Micro-complement fixation in Klebsiella classification.

    abstract::The alkaline phosphatases of 29 strains of bacteria assigned by various authors to the genera Aerobacter, Klebsiella and Enterobacter were compared by the micro-complement fixation technique. On the basis of phosphatase resemblance, we recommend that all strains hitherto assigned to Aerobacter aerogenes and Enterobact...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.110.3.803-808.1972

    authors: Steffen DL,Cocks GT,Wilson AC

    更新日期:1972-06-01 00:00:00

  • Cloning, sequencing, and expression of the gene encoding cyclic 2, 3-diphosphoglycerate synthetase, the key enzyme of cyclic 2, 3-diphosphoglycerate metabolism in Methanothermus fervidus.

    abstract::Cyclic 2,3-diphosphoglycerate synthetase (cDPGS) catalyzes the synthesis of cyclic 2,3-diphosphoglycerate (cDPG) by formation of an intramolecular phosphoanhydride bond in 2,3-diphosphoglycerate. cDPG is known to be accumulated to high intracellular concentrations (>300 mM) as a putative thermoadapter in some hyperthe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.22.5997-6004.1998

    authors: Matussek K,Moritz P,Brunner N,Eckerskorn C,Hensel R

    更新日期:1998-11-01 00:00:00

  • ompT encodes the Escherichia coli outer membrane protease that cleaves T7 RNA polymerase during purification.

    abstract::Bacteriophage T7 RNA polymerase is stable in Escherichia coli but very susceptible to cleavage by at least one endoprotease after cell lysis. The major source of this endoprotease activity was found to be localized to the outer membrane of the cell. A rapid whole-cell assay was developed to screen different strains fo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.3.1245-1253.1988

    authors: Grodberg J,Dunn JJ

    更新日期:1988-03-01 00:00:00

  • Biosynthesis of uridine diphosphate N-acetylmannosaminuronic acid in rff mutants of Salmonella tryphimurium.

    abstract::In Salmonella typhimurium, three groups of genes located in rfb, rfe, and rff clusters are known to be involved in the biosynthesis of the enterobacterial common antigen. We found that enzymatic synthesis of uridine diphosphate N-acetylmannosaminouric acid, the activated form of a constituent sugar of the common antig...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.136.1.227-233.1978

    authors: Lew HC,Nikaido H,Mäkelä PH

    更新日期:1978-10-01 00:00:00

  • Subunit interactions and protein stability in the cyanobacterial light-harvesting proteins.

    abstract::Strain 4R is a phycocyanin-minus mutant of the unicellular cyanobacterium Synechocystis sp. strain 6803. Although it lacks the light-harvesting protein phycocyanin, 4R has normal levels of phycocyanin (cpc) transcripts. Sequence analysis of the cpcB gene encoding the phycocyanin beta subunit shows an insertion mutatio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.23.6798-6803.1995

    authors: Plank T,Toole C,Anderson LK

    更新日期:1995-12-01 00:00:00

  • Identification of YidC residues that define interactions with the Sec Apparatus.

    abstract::In bacteria, a subset of membrane proteins insert into the membrane via the Sec apparatus with the assistance of the widely conserved essential membrane protein insertase YidC. After threading into the SecYEG translocon, transmembrane segments of nascent proteins are thought to exit the translocon via a lateral gate i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01095-13

    authors: Li Z,Boyd D,Reindl M,Goldberg MB

    更新日期:2014-01-01 00:00:00

  • Physical properties of L-asparaginase from Serratia marcescens.

    abstract::Purified L-asparaginase from Serratia marcescens had an apparent-weight average molecular weight of 171,000 to 180,000 as determined by electrophoresis on polyacrylamide gels and by sedimentation equilibrium at low speed in an analytical ultracentrifuge. A subunit molecular weight of 31,500 +/- 1,500 was estimated for...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.125.2.719-727.1976

    authors: Stern ML,Phillips AW,Gottlieb AJ

    更新日期:1976-02-01 00:00:00

  • Regulation of ergosterol biosynthesis and sterol uptake in a sterol-auxotrophic yeast.

    abstract::Inhibition of sterol uptake in Saccharomyces cerevisiae sterol auxotroph FY3 (alpha hem1 erg7 ura) by delta-aminolevulinic acid (ALA) is dependent on the ability of the organism to synthesize heme from ALA. Sterol-depleted cells not exposed to ALA or strain PFY3 cells, with a double heme mutation, exposed to ALA did n...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.8.3707-3711.1987

    authors: Lorenz RT,Parks LW

    更新日期:1987-08-01 00:00:00

  • Dosage of the smallest chromosome affects both the yeast-hyphal transition and the white-opaque transition of Candida albicans WO-1.

    abstract::The WO-1 strain of Candida albicans is capable of alternating between two highly distinct yeast cell types termed white and opaque (E. H. A. Rikkerrink, B. B. Magee, and P. T. Magee, J. Bacteriol. 170:895-899, 1988; B. Slutsky, M. Staebell, J. Anderson, L. Risen, M. Pfaller, and D. R. Soll, J. Bacteriol. 169:189-197, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.23.7436-7442.1991

    authors: McEachern MJ,Hicks JB

    更新日期:1991-12-01 00:00:00

  • Mode of action of yeast toxins: energy requirement for Saccharomyces cerevisiae killer toxin.

    abstract::The role of the energy status of the yeast cell in the sensitivity of cultures to two yeast toxins was examined by using 12K release from cells as a measure of toxin action. The Saccharomyces cerevisiae killer toxin bound to sensitive cells in the presence of drugs that interfered with the generation or use of energy,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.129.2.668-677.1977

    authors: Skipper N,Bussey H

    更新日期:1977-02-01 00:00:00

  • NosR, a membrane-bound regulatory component necessary for expression of nitrous oxide reductase in denitrifying Pseudomonas stutzeri.

    abstract::The regulatory element NosR was identified within the nos region of the denitrification gene cluster of Pseudomonas stutzeri ZoBell (ATCC 14405) and characterized. It is essential for expression of the N2O reductase encoded by nosZ immediately downstream of nosR. The nosR region was initially identified by Tn5 mutagen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.16.5332-5339.1992

    authors: Cuypers H,Viebrock-Sambale A,Zumft WG

    更新日期:1992-08-01 00:00:00

  • Deoxyribonucleate binding and transformation in Rhizobium jpaonicum.

    abstract::Rhizobium japonicum, capable of binding high-molecular-weight donor (32)P-labeled deoxyribonucleic acid (DNA) during late log phase in a competence medium, was transformed for streptomycin resistance with a frequency of transformation ranging between 0.02 and 0.08%. Eight to 10% of the homologous native (32)P-labeled ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.111.2.356-360.1972

    authors: Raina JL,Modi VV

    更新日期:1972-08-01 00:00:00

  • Factors affecting growth and nitrogen fixation of Spirillum lipoferum.

    abstract::Spirillum lipoferum grows vigorously on malate, succinate, lactate, or pyruvate, moderately on galactose or acetate, and poorly on glucose or citrate. It reduces 15N2. Acetylene reduction rates decrease rapidly when the pH of the culture rises above 7.8. The organism is highly aerobic and had doubling times as low as ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.127.3.1248-1254.1976

    authors: Okon Y,Albrecht SL,Burris RH

    更新日期:1976-09-01 00:00:00

  • Control of mucoidy in Pseudomonas aeruginosa: transcriptional regulation of algR and identification of the second regulatory gene, algQ.

    abstract::A new alginate regulatory gene, algQ, was identified in a chromosomal region which, when tandemly amplified, induces mucoidy in Pseudomonas aeruginosa. The algQ gene was found closely linked to the previously identified algR gene. Both algQ and algR were required for transcription of the key alginate biosynthetic gene...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.7.3680-3688.1989

    authors: Deretic V,Konyecsni WM

    更新日期:1989-07-01 00:00:00

  • Direction of deoxyribonucleic acid transfer and replication in a derivative of plasmid R100-1.

    abstract::The site of integration and the molecular orientation of a prophage Mu integrated within the resistance transfer factor component of plasmid R100-1 have been determined on the physical map of the plasmid. This allowed us (i) to determine the direction of deoxyribonucleic acid transfer from oriT during conjugation and ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.140.3.1105-1108.1979

    authors: Legrand P,Bouche JP,Louarn JM

    更新日期:1979-12-01 00:00:00

  • Taxonomy of marine bacteria: Beneckea parahaemolytica and Beneckea alginolytica.

    abstract::A collection of 169 strains, including 91 obtained from cases of gastroenteritis and 41 from localized tissue infections and infections of the eye and ear, was submitted to an extensive nutritional, physiological, and morphological characterization. The nutritional and physiological data obtained from these strains, a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.113.3.1144-1155.1973

    authors: Baumann P,Baumann L,Reichelt JL

    更新日期:1973-03-01 00:00:00

  • Cells of Escherichia coli contain a protein-tyrosine kinase, Wzc, and a phosphotyrosine-protein phosphatase, Wzb.

    abstract::Two proteins of Escherichia coli, termed Wzc and Wzb, were analyzed for their capacity to participate in the reversible phosphorylation of proteins on tyrosine. First, Wzc was overproduced from its specific gene and purified to homogeneity by affinity chromatography. Upon incubation in the presence of radioactive ATP,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.11.3472-3477.1999

    authors: Vincent C,Doublet P,Grangeasse C,Vaganay E,Cozzone AJ,Duclos B

    更新日期:1999-06-01 00:00:00

  • Delayed expression of in vivo restriction activity following conjugal transfer of Escherichia coli hsdK (restriction-modification) genes.

    abstract::Following conjugal transfer of the hsdK genes (hsdRK, hsdMK, and hsdSK) of Escherichia coli K-12, restriction activity was first detected only after approximately 15 generations, whereas modification activity was observed immediately. This sequential expression explains the establishment of hsdK genes in a nonmodified...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.15.4905-4906.1993

    authors: Prakash-Cheng A,Ryu J

    更新日期:1993-08-01 00:00:00

  • Molecular nature of two nonconjugative plasmids carrying drug resistance genes.

    abstract::Two nonconjugative R-plasmids, N-SuSm and N-Tc, have been characterized. Both were of relatively small size (5 x 10(6) to 6 x 10(6) daltons) and present in multiple copies within their respective bacterial hosts. N-SuSm possessed a guanine plus cytosine content of 55%, whereas N-Tc was 49% guanine plus cytosine. Altho...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.117.2.619-630.1974

    authors: Guerry P,van Embden J,Falkow S

    更新日期:1974-02-01 00:00:00

  • The plant pathogen Pantoea ananatis produces N-acylhomoserine lactone and causes center rot disease of onion by quorum sensing.

    abstract::A number of gram-negative bacteria have a quorum-sensing system and produce N-acyl-l-homoserine lactone (AHL) that they use them as a quorum-sensing signal molecule. Pantoea ananatis is reported as a common colonist of wheat heads at ripening and causes center rot of onion. In this study, we demonstrated that P. anana...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01054-07

    authors: Morohoshi T,Nakamura Y,Yamazaki G,Ishida A,Kato N,Ikeda T

    更新日期:2007-11-01 00:00:00

  • Sequence of b cytochromes relative to ubiquinone in the electron transport chain of Escherichia coli.

    abstract::A ubiquinone-deficient mutant, carrying mutations in two genes affecting ubiquinone biosynthesis, has been used, in comparison with a normal strain, to determine the sequence of some of the components of the electron transport chain of Escherichia coli. The amounts of cytochromes reduced during aerobic steady-state co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.133.2.477-484.1978

    authors: Downie JA,Cox GB

    更新日期:1978-02-01 00:00:00

  • Genetic evidence for the action of oxathiin and thiazole derivatives on the succinic dehydrogenase system of Ustilago maydis mitochondria.

    abstract::The inhibitory effect of fungitoxic derivatives of 1,4-oxathiin on substrate oxidation by the basidiomycete Ustilago maydis is diminished by a single-gene mutation (oxr). The difference between mutant and wild type is approximately the same on the basis of inhibition of either growth and operation of the tricarboxylic...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.110.3.809-817.1972

    authors: Georgopoulos SG,Alexandri E,Chrysayi M

    更新日期:1972-06-01 00:00:00

  • Genetic analysis of the chitinase system of Serratia marcescens 2170.

    abstract::To carry out a genetic analysis of the degradation and utilization of chitin by Serratia marcescens 2170, various Tn5 insertion mutants with characteristic defects in chitinase production were isolated and partially characterized. Prior to the isolation of the mutants, proteins secreted into culture medium in the pres...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.22.7111-7117.1997

    authors: Watanabe T,Kimura K,Sumiya T,Nikaidou N,Suzuki K,Suzuki M,Taiyoji M,Ferrer S,Regue M

    更新日期:1997-11-01 00:00:00