Molecular nature of two nonconjugative plasmids carrying drug resistance genes.

Abstract:

:Two nonconjugative R-plasmids, N-SuSm and N-Tc, have been characterized. Both were of relatively small size (5 x 10(6) to 6 x 10(6) daltons) and present in multiple copies within their respective bacterial hosts. N-SuSm possessed a guanine plus cytosine content of 55%, whereas N-Tc was 49% guanine plus cytosine. Although these plasmids were inherently nontransmissible they could be mobilized by a large variety of transfer agents including Ent, Hly, and K88. The fi(-) transfer factors tested were far more likely (about 200x) to mobilize these nonconjugative plasmids than were the fi(+) transfer factors tested. Although the mobilization phenomenon was not found to be associated with a detectable level of direct stable recombinational union between N-SuSm or N-Tc with a transfer factor, we were able to demonstrate a low level of recombination between these replicons and a transfer factor by P1-mediated transduction. The isolation of recombinants between transfer factors and nonconjugative plasmids presumably represents one means by which unitary molecular types of R-plasmids arise and by which existing R-plasmids may acquire new resistance determinants.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Guerry P,van Embden J,Falkow S

doi

10.1128/JB.117.2.619-630.1974

subject

Has Abstract

pub_date

1974-02-01 00:00:00

pages

619-30

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

117

pub_type

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